Meiotic chromosome behavior in spread preparations of yeast

Chromosome behavior in meiosis is well characterized from cytological and genetic descriptions but little is known of the underlying molecular mechanisms, largely because no one experimental system has been developed to support an integrated application of modern cytological, genetic, and molecular biological methods. To combine efficient analyses of meiotic chromosome structure and function in a single organism, we have extended to yeast methods for making spread preparations of nuclei. Features of yeast meiosis that parallel meiosis in large eukaryotes, such as bouquet formation and prophase chromosome condensation that occurs in concert with synaptonemal complex formation, are evident for the first time. The ability to analyze large numbers of nuclei at the light and electron microscopes in preparations amenable to a variety of cytological and immunocytological techniques will facilitate the description of meiosis at the molecular level in yeast.

• Publication year

  1988

• Venue

  Journal of Cell Biology

• Publication date

  1988-03-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.106.3.567PMID 2450094PMCID 2115091
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Meiotic cytology of Saccharomyces cerevisiae in protoplast lysates

  2004cited by this paper
• The synaptonemal complex and the spindle plaque during meiosis in yeast

  2004cited by this paper
• Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus)

  2004cited by this paper
• Synaptonemal complexes of normal and mutant yeast chromosomes (Saccharomyces cerevisiae)

  2004cited by this paper
• Synaptonemal complex antigen location and conservation

  1987cited by this paper
• Meiotic gene conversion and crossing over between dispersed homologous sequences occurs frequently in Saccharomyces cerevisiae.

  1987cited by this paper
• 8 – The Synaptonemal Complex and Meiosis: An Immunocytochemical Approach

  1987cited by this paper
• Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes.

  1986cited by this paper
• Genetic map of Saccharomyces cerevisiae, edition 9.

  1985cited by this paper
• Evolution of the dispersed SUC gene family of Saccharomyces by rearrangements of chromosome telomeres

  1985cited by this paper
• Genetic map of Saccharomyces cerevisiae, edition 9

  1985influential reference
• Synapsis, synaptic adjustment and DNA synthesis in mouse oocytes

  1984cited by this paper
• Meiotic karyotype of the yeast Saccharomyces cerevisiae.

  1984cited by this paper
• Morphological and temporal sequence of meiotic prophase development at puberty in the male mouse.

  1984cited by this paper
• Rearrangements of highly polymorphic regions near telomeres of Saccharomyces cerevisiae

  1984cited by this paper
• The synaptonemal complex in genetic segregation.

  1984cited by this paper
• Composition and role of the synaptonemal complex.

  1984cited by this paper
• Unusual DNA sequences associated with the ends of yeast chromosomes

  1984cited by this paper
• The new yeast genetics

  1983cited by this paper
• Organization of DNA sequences and replication origins at yeast telomeres.

  1983cited by this paper
• Yeast genetics : fundamental and applied aspects

  1983cited by this paper
• Analysis of the junction between ribosomal RNA genes and single-copy chromosomal sequences in the yeast Saccharomyces cerevisiae.

  1982cited by this paper
• Recombination in yeast ribosomal DNA

  1982cited by this paper
• Meiosis and Ascospore Development

  1981cited by this paper
• Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method

  1980cited by this paper
• Autonomously replicating sequences in Saccharomyces cerevisiae.

  1980cited by this paper
• Isolation of SPO12-1 and SPO13-1 from a natural variant of yeast that undergoes a single meiotic division.

  1980cited by this paper
• Morphogenesis of the synapton during yeast meiosis

  1979cited by this paper
• Chromatin behaviour during the mitotic cell cycle of Saccharomyces cerevisiae.

  1977cited by this paper
• Electron microscopic observations on the meiotic karyotype of diploid and tetraploid Saccharomyces cerevisiae.

  1975cited by this paper
• The synaptinemal complex.

  1972cited by this paper
• SPINDLES, SPINDLE PLAQUES, AND MEIOSIS IN THE YEAST SACCHAROMYCES CEREVISIAE (HANSEN)

  1971cited by this paper
• Synaptic structures in the nuclei of sporulating yeast, Saccharomyces cerevisiae (Hansen).

  1971cited by this paper
• The Cell in Development and Heredity

  1925influential reference
• The cell in development and heredity

  year unknowncited by this paper

• Nucleolar Cdc14 Splitting Reflects Recombination Context and Meiotic Chromosome Dynamics

  2025cites this paper
• Dynamic configurations of meiotic DNA-break hotspot determinant proteins.

  2022cites this paper
• Getting there: understanding the chromosomal recruitment of the AAA+ ATPase Pch2/TRIP13 during meiosis

  2021influential citation
• Defining meiotic recombination landscape in tomato

  2021cites this paper
• Dynamic configurations of meiotic hotspot determinants

  2020influential citation
• Disassembly of the synaptonemal complex in chicken oocytes analyzed by super-resolution microscopy

  2019cites this paper
• Meiotic chromosomes in motion: a perspective from Mus musculus and Caenorhabditis elegans

  2019cites this paper
• A ZIP1 Separation-of-Function Allele Reveals that Meiotic Centromere Pairing Drives Meiotic Segregation of Achiasmate Chromosomes in Budding Yeast

  2018cites this paper
• A ZIP1 separation-of-function allele reveals that centromere pairing drives meiotic segregation of achiasmate chromosomes in budding yeast

  2018cites this paper
• Spermatocyte Spreading during Meiotic Cell Preparation is a Two StepProcess

  2017cites this paper
• Yeast Chromosome Dynamics Revealed by Immuno FISH

  2017cites this paper
• Prophase I: Preparing Chromosomes for Segregation in the Developing Oocyte.

  2017cites this paper
• The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization

  2017cites this paper
• Meiotic Centromere Coupling and Pairing Function by Two Separate Mechanisms in Saccharomyces cerevisiae

  2016cites this paper
• Ipl1/Aurora-B is necessary for kinetochore restructuring in meiosis I in Saccharomyces cerevisiae

  2015cites this paper
• Surface Spreading and Immunostaining of Yeast Chromosomes.

  2015cites this paper
• The synaptonemal complex is assembled by a polySUMOylation-driven feedback mechanism in yeast

  2015cites this paper
• Microscopy techniques to examine DNA replication in fission yeast.

  2015cites this paper
• A selfish DNA element engages a meiosis-specific motor and telomeres for germ-line propagation

  2014cites this paper
• Unravelling the proteomic profile of rice meiocytes during early meiosis

  2014influential citation
• Microtubules move the nucleus to quiescence

  2014influential citation
• Responses to Low Double-Strand Break Levels in Budding Yeast Meiosis

  2013cites this paper
• Dual mechanisms prevent premature chromosome segregation during meiosis

  2013cites this paper
• SUMO Localizes to the Central Element of the Synaptonemal Complex and is Essential for Meiotic Chromosome Synapsis in Saccharomyces cerevisiae

  2013cites this paper
• Meiotic Crossover Control by Concerted Action of Rad51-Dmc1 in Homolog Template Bias and Robust Homeostatic Regulation

  2013cites this paper
• The Sum1/Ndt80 Transcriptional Switch and Commitment to Meiosis in Saccharomyces cerevisiae

  2012cites this paper
• all four products of a single meiosis erecta meiosis and meiotic recombination by resequencing Landsberg genome-wide variations before and after Arabidopsis Analysis of Material

  2012cites this paper
• Analysis of Arabidopsis genome-wide variations before and after meiosis and meiotic recombination by resequencing Landsberg erecta and all four products of a single meiosis.

  2012cites this paper
• Meiotic Chromosome Pairing Is Promoted by Telomere-Led Chromosome Movements Independent of Bouquet Formation

  2012cites this paper
• A Peek into the Complex Realm of Histone Phosphorylation

  2011cites this paper
• Temporal Characterization of Homology-Independent Centromere Coupling in Meiotic Prophase

  2010cites this paper
• Meiotic Cohesin Promotes Pairing of Nonhomologous Centromeres in Early Meiotic Prophase

  2010cites this paper
• FISH as a tool to investigate chromosome behavior in budding yeast.

  2010cites this paper
• FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast

  2009cites this paper
• Assaying chromosome pairing by FISH analysis of spread Saccharomyces cerevisiae nuclei.

  2009cites this paper
• Pds5 is required for homologue pairing and inhibits synapsis of sister chromatids during yeast meiosis

  2009cites this paper
• Chromosome spreading and immunofluorescence methods in Saccharomyes cerevisiae.

  2009cites this paper
• Microscopy techniques to examine DNA replication in fission yeast.

  2009cites this paper
• Fluorescence In Situ Hybridization (FISH) — Application Guide

  2009cites this paper
• The Synaptonemal Complex Protein Zip1 Promotes Bi-Orientation of Centromeres at Meiosis I

  2009cites this paper
• Yeast Pch2 promotes domainal axis organization, timely recombination progression, and arrest of defective recombinosomes during meiosis

  2008cites this paper
• Synaptonemal complex analysis in spermatocytes of diploid and triploid Chinese shrimp Fenneropenaeus chinensis.

  2008cites this paper
• Phosphorylation of the axial element protein Hop1 by Mec1/Tel1 ensures meiotic interhomolog recombination.

  2008cites this paper
• Prelude to a division.

  2008cites this paper
• Synaptic and Recombination Nodules in Mammals: Structural Continuity with Shifting Protein Composition

  2007cites this paper
• MPS3 mediates meiotic bouquet formation in Saccharomyces cerevisiae

  2007cites this paper
• A Novel Nonnull ZIP1 Allele Triggers Meiotic Arrest With Synapsed Chromosomes in Saccharomyces cerevisiae

  2007cites this paper
• Chiasma formation: chromatin/axis interplay and the role(s) of the synaptonemal complex

  2006cites this paper
• Mitotic chromosome structure and condensation.

  2006cites this paper
• Molecular aspects of meiotic chromosome synapsis and recombination.

  2005cites this paper
• H2B (Ser10) Phosphorylation is Induced during Apoptosis and Meiosis in S. cerevisiae

  2005cites this paper
• Budding yeast PDS5 plays an important role in meiosis and is required for sister chromatid cohesion

  2005cites this paper
• Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae

  2005cites this paper
• Recombination nodules in plants

  2005cites this paper
• Correlating chromatin states with silencing phenotypes in "Saccharomyces cerevisiae".

  2005cites this paper
• Differential association of SMC1α and SMC3 proteins with meiotic chromosomes in wild-type and SPO11-deficient male mice

  2004cites this paper
• A model for chromosome structure during the mitotic and meiotic cell cycles

  2004cites this paper
• Monitoring changes in the subcellular location of proteins in S. cerevisiae.

  2004cites this paper
• Conditional hyporecombination mutants of three REC genes of Saccharomyces cerevisiae

  2004cites this paper
• Tying synaptonemal complex initiation to the formation and programmed repair of DNA double-strand breaks.

  2004cites this paper
• Meiotic condensin is required for proper chromosome compaction, SC assembly, and resolution of recombination-dependent chromosome linkages

  2003cites this paper
• Depletion of H2A-H2B dimers in Saccharomyces cerevisiae triggers meiotic arrest by reducing IME1 expression and activating the BUB2-dependent branch of the spindle checkpoint.

  2003cites this paper
• Increased ploidy and KAR3 and SIR3 disruption alter the dynamics of meiotic chromosomes and telomeres

  2003cites this paper
• Chromosomes of the budding yeast Saccharomyces cerevisiae.

  2003cites this paper
• A Method for Preparation of Synaptonemal Complexes of Meiotic Chromosomes from Basidial Protoplasts of the White Button Mushroom Agaricus bisporus (Lange) Imbach.

  2003cites this paper
• FISH Technology

  2002cites this paper
• Homologous chromosome associations and nuclear order in meiotic and mitotically dividing cells of budding yeast.

  2002cites this paper
• A history of research on yeasts 4: cytology part II, 1950–1990

  2002cites this paper
• Zipl-induced Changes in Synaptonemal Complex Structure and l lycomplex Assembly

  2002cites this paper
• Physical and functional interactions among basic chromosome organizational features govern early steps of meiotic chiasma formation.

  2002cites this paper
• Analysis of germline mutations induced by chemicals

  2002cites this paper
• Yeast FISH: Delineation of Chromosomal Targets in Vegetative and Meiotic Yeast Cells

  2002cites this paper
• The s q l Mutant of Saccharomyces cereukiae Arrests in Pachytene and Is Deficient in Meiotic Recombination

  2002cites this paper
• Functions in a Saccharomyces cerhsiae Meiotic Pathway That Is Largely Independent of the W . 5 1 Pathway

  2002cites this paper
• Induced by tRNA Ochre Suppressors

  2002cites this paper
• Progression Into the First Meiotic Division Is Sensitive to Histone HN-HZB Dimer Concentration in Saccharomyces cer&ae

  2002cites this paper
• Materials and Methods Yeast Subcellular Fractionation : Preparation of Enriched Nuclei and Highly Enriched NPCs

  2002cites this paper
• Multiple roles of Spo11 in meiotic chromosome behavior

  2000cites this paper
• Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes.

  2000cites this paper
• Methods for immunoelectron microscopic and fine structural analysis of synaptonemal complexes and nodules in yeast

  2000cites this paper
• Meiotic chromosome behavior in Saccharomyces cerevisiae and (mostly) mammals.

  2000cites this paper
• Meiotic Telomere Protein Ndj1p Is Required for Meiosis-Specific Telomere Distribution, Bouquet Formation and Efficient Homologue Pairing

  2000cites this paper
• Bouquet formation in budding yeast: initiation of recombination is not required for meiotic telomere clustering.

  1999cites this paper
• Pch2 links chromatin silencing to meiotic checkpoint control.

  1999cites this paper
• Meiotic chromosomes: integrating structure and function.

  1999cites this paper
• Zip2, a meiosis-specific protein required for the initiation of chromosome synapsis.

  1998cites this paper
• Genetic and morphological approaches for the analysis of meiotic chromosomes in yeast.

  1998cites this paper
• Functions of DNA repair genes during meiosis.

  1998cites this paper
• The leptotene-zygotene transition of meiosis.

  1998cites this paper
• The meiosis-specific Hop2 protein of S. cerevisiae ensures synapsis between homologous chromosomes.

  1998cites this paper
• Caught in the act: deducing meiotic function from protein immunolocalization.

  1998cites this paper
• Telomere Functions in the Translocation Heterozygote, Rhoeo spathacea

  1998cites this paper
• Yeast Nuclei Display Prominent Centromere Clustering That Is Reduced in Nondividing Cells and in Meiotic Prophase

  1998cites this paper
• Identification and Characterization of Mlp1p and Mlp2p : Molecular Components of Filaments Localized at the Interface between the Nuclear Pore Complex and Nuclear Interior

  1998cites this paper
• Meiotic chromosome morphology and behavior in zip1 mutants of Saccharomyces cerevisiae.

  1998cites this paper
• 4 Functions of DNA Repair Genes during Meiosis

  1997cites this paper
• Telomeres Cluster De Novo before the Initiation of Synapsis: A Three-dimensional Spatial Analysis of Telomere Positions before and during Meiotic Prophase

  1997cites this paper
• The Yeast Motor Protein, Kar3p, Is Essential for Meiosis I

  1997cites this paper
• Tam1, a telomere-associated meiotic protein, functions in chromosome synapsis and crossover interference.

  1997cites this paper
• Progression into the first meiotic division is sensitive to histone H2A-H2B dimer concentration in Saccharomyces cerevisiae.

  1997cites this paper