Is the mammalian cell plasma membrane a barrier to oxygen transport?

Oxygen transport in the Chinese hamster ovary (CHO) plasma membrane has been studied by observing the collision of molecular oxygen with nitroxide radical spin labels placed in the lipid bilayer portion of the membrane at various distances from the membrane surface using the long-pulse saturation-recovery electron spin resonance (ESR) technique. The collision rate was estimated for 5-, 12-, and 16-doxylstearic acids from spin-lattice relaxation times (T1) measured in the presence and absence of molecular oxygen. Profiles of the local oxygen transport parameters across the membrane were obtained showing that the oxygen diffusion-concentration product is lower than in water for all locations at 37 degrees C. From oxygen transport parameter profiles, the membrane oxygen permeability coefficients were estimated according to the procedure developed earlier by Subczynski et al. (Subczynski, W. K., J. S. Hyde, and A. Kusumi. 1989. Proceedings of the National Academy of Sciences, USA. 86:4474-4478). At 37 degrees C, the oxygen permeability coefficient for the plasma membrane was found to be 42 cm/s, about two times lower than for a water layer of the same thickness as the membrane. The oxygen concentration difference across the CHO plasma membrane at physiological conditions is in the nanomolar range. It is concluded that oxygen permeation across the cell plasma membrane cannot be a rate-limiting step for cellular respiration. Correlations of the form PM = cKs between membrane permeabilities PM of small nonelectrolyte solutes of mol wt less than 50, including oxygen, and their partition coefficients K into hexadecane and olive oil are reported. Hexadecane: c = 26 cm/s, s = 0.95; olive oil: c = 23 cm/s, s = 1.56. These values of c and s differ from those reported in the literature for solutes of 50 less than mol wt less than 300 (Walter, A., and J. Gutknecht. 1986. Journal of Membrane Biology. 90:207-217). It is concluded that oxygen permeability through membranes can be reliably predicted from measurement of partition coefficients.

• Publication year

  1992

• Venue

  The Journal of General Physiology

• Publication date

  1992-07-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1085/JGP.100.1.69PMID 1324973PMCID 2229127
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• For small nonelectrolyte solutes below 50 Da, membrane permeability follows correlations with partition coefficients into hexadecane and olive oil, with distinct fitted c and s values for each solvent.

  Confidence 0.95

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• The oxygen permeability coefficient of the CHO plasma membrane at 37 °C is about 42 cm/s, roughly twofold lower than that of a water layer of the same thickness, implying that membrane oxygen permeation is not rate-limiting for cellular respiration.

  Confidence 0.99

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• The oxygen diffusion-concentration product is lower than in water at all measured locations in the CHO plasma membrane at 37 °C.

  Confidence 0.97

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• Long-pulse saturation-recovery ESR on 5-, 12-, and 16-doxylstearic acids yielded depth-dependent local oxygen transport parameters across the CHO plasma membrane.

  Confidence 0.98

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review

• 5-, 12-, and 16-doxylstearic acids

  spin label, chemical compound

  Membrane spin-label probes positioned at different depths in the lipid bilayer by the doxyl group location.

  Aliases: 5-DSA, 12-DSA, 16-DSA

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• cho plasma membrane

  biological membrane

  The plasma membrane of Chinese hamster ovary cells examined for oxygen transport properties.

  Aliases: Chinese hamster ovary plasma membrane

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• hexadecane

  solvent

  A hydrocarbon solvent used as one of the partitioning phases in the permeability correlation.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• local oxygen transport parameters

  parameter

  Depth-dependent transport quantities derived from relaxation measurements to characterize oxygen behavior in the membrane.

  Aliases: oxygen transport parameters

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• long-pulse saturation-recovery esr

  measurement technique

  An electron spin resonance method used here to measure spin-lattice relaxation times after pulse saturation.

  Aliases: saturation-recovery ESR

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• membrane permeability coefficient

  coefficient

  The permeability of a solute across a membrane, denoted PM in the abstract.

  Aliases: PM

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• olive oil

  solvent

  A lipid-rich solvent used as another partitioning phase in the permeability correlation.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• oxygen diffusion-concentration product

  parameter

  A combined transport parameter representing the product of oxygen diffusivity and local concentration in the membrane.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• oxygen permeability coefficient

  coefficient

  A coefficient used to quantify oxygen passage across the membrane.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• partition coefficient

  parameter

  A measure of how a solute distributes between two phases, used here for hexadecane and olive oil.

  Aliases: K

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• small nonelectrolyte solutes

  solute class

  Neutral low-molecular-weight solutes, here restricted to compounds below about 50 Da in the permeability analysis.

  Aliases: small nonelectrolytes, solutes <50 Da

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• water layer

  reference medium

  A thickness-matched aqueous reference layer used for comparing membrane oxygen permeability.

  Aliases: aqueous layer

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review

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