Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose.

No abstract is available for this paper.

• Publication year

  1972

• Venue

  Biochemistry

• Publication date

  1972-06-06

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/BI00762A013PMID 5028497
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Effect of the microenvironment on the mode of action of immobilized enzymes.

  2006cited by this paper
• [31] Affinity chromatography

  1971cited by this paper
• Affinity chromatography.

  1971cited by this paper
• The purification of beta-galactosidase from Escherichia coli by affinity chromatography.

  1971cited by this paper
• Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

  1970cited by this paper
• Trypsin and Papain Covalently Coupled to Porous Glass: Preparation and Characterization

  1969cited by this paper
• Urease covalently coupled to porous glass.

  1969cited by this paper
• Alkaline Phosphatase insolubilized by Covalent Linkage to Porous Glass

  1969cited by this paper
• Sweden: Molecular Sieving and Adsorption

  1968cited by this paper
• A new convenient reagent for peptide syntheses.

  1968cited by this paper
• Selective enzyme purification by affinity chromatography.

  1968cited by this paper
• Chemical Coupling of Proteins to Agarose

  1967cited by this paper
• Water-insoluble derivatives of enzymes, antigens, and antibodies.

  1966cited by this paper
• A WATER-INSOLUBLE POLYANIONIC DERIVATIVE OF TRYPSIN. II. EFFECT OF THE POLYELECTROLYTE CARRIER ON THE KINETIC BEHAVIOR OF THE BOUND TRYPSIN.

  1964cited by this paper
• Notes- A Convenient Synthesis of Water-Soluble Carbodiimides.

  1961cited by this paper
• Automatic recording apparatus for use in the chromatography of amino acids.

  1958cited by this paper
• [3] Trypsinogen and trypsin

  1955cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper
• The specific esterase activity of trypsin.

  1948cited by this paper
• The Determination of Enzyme Dissociation Constants

  1934cited by this paper

• Addressing NHS Chemistry: Efficient Quenching of Excess TMT Reagent and Reversing TMT Overlabeling in Proteomic Samples by Methylamine

  2025cites this paper
• Studies of the amidation of porphyrin-NHS esters in dilute aqueous solution

  2025cites this paper
• A Kinetically Controlled Bioconjugation Method for the Synthesis of Radioimmunoconjugates and the Development of a Domain Mapping MS-Workflow for Its Characterization

  2024influential citation
• 3-Acyl-4-Pyranone as a Lysine Residue-Selective Bioconjugation Reagent for Peptide and Protein Modification.

  2024cites this paper
• Removal of NHS-labelling By-products in Proteomic Samples

  2024influential citation
• Ordered immobilization of serine proteases enabled by a linchpin directed modification platform.

  2024cites this paper
• Optimizing lipid nanoparticles for fetal gene delivery in vitro, ex vivo, and aided with machine learning.

  2024cites this paper
• Thermodynamic Overview of Bioconjugation Reactions Pertinent to Lysine and Cysteine Peptide and Protein Residues

  2023cites this paper
• A guide to functionalisation and bioconjugation strategies to surface-initiated polymer brushes

  2023cites this paper
• Integration of functional peptides into nucleic acid-based nanostructures.

  2023cites this paper
• An Automatic Immunoaffinity Pretreatment of Deoxynivalenol Coupled with UPLC-UV Analysis

  2022cites this paper
• Chemical modification of hyaluronic acid improves its supportive action on embryo implantation.

  2022cites this paper
• Fabrication Strategies Towards Hydrogels for Biomedical Application: Chemical and Mechanical Insights

  2022cites this paper
• A membrane cytoskeleton from Dictyostelium discoideum. II. A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F- Integral proteins mediate the binding of plasma membranes to F-actin affinity beads actin affinity beads

  2022cites this paper
• Recent advance in solid state nanopores modification and characterization.

  2022cites this paper
• Electrochemical and X-ray Photoelectron Spectroscopy Surface Characterization of Interchain-Driven Self-Assembled Monolayer (SAM) Reorganization

  2022cites this paper
• Efficient Amino-Sulfhydryl Stapling on Peptides and Proteins Using Bifunctional NHS-Activated Acrylamides.

  2021cites this paper
• Advances in Functionalized Photosensitive Polymeric Nanocarriers

  2021cites this paper
• pH Dependence of Succinimide-Ester-Based Protein Cross-Linking for Structural Mass Spectrometry Applications

  2021cites this paper
• Chondrogenesis of Mesenchymal Stem Cells through Local Release of TGF-β3 from Heparinized Collagen Biofabric

  2021cites this paper
• Azaphilones as Activation-Free Primary-Amine-Specific Bioconjugation Reagents for Peptides, Proteins and Lipids.

  2021cites this paper
• Comparison of two fluorescence quantitative immunochromatographic assays for the detection of amantadine in chicken muscle.

  2021cites this paper
• Organic building blocks at inorganic nanomaterial interfaces.

  2021cites this paper
• Fast-dissociating but highly specific antibodies are novel tools in biology, especially useful for multiplex super-resolution microscopy

  2021cites this paper
• Tris(hydroxymethyl)aminomethane Compatibility with N-Hydroxysuccinimide Ester Chemistry: Biotinylation of Peptides and Proteins in TRIS Buffer.

  2021cites this paper
• Direct Conjugation of Streptavidin to Encoded Hydrogel Microparticles for Multiplex Biomolecule Detection with Rapid Probe-Set Modification

  2020cites this paper
• Preparation, Characterization, and Radiolabeling of [68Ga]Ga-NODAGA-Pamidronic Acid: A Potential PET Bone Imaging Agent

  2020cites this paper
• Covalent Immobilization of L-Asparaginase and Optimization of Its Enzyme Reactor for Reducing Acrylamide Formation in a Heated Food Model System

  2020cites this paper
• N-Hydroxysulfosuccinimide Esters versus Thiomers: A Comparative Study Regarding Mucoadhesiveness.

  2019cites this paper
• Temperature-driven Metalloprotein-based Hybrid Hydrogels for Selective and Reversible Removal of Cadmium(II) from Water.

  2019cites this paper
• AJICAP: Affinity Peptide Mediated Regiodivergent Functionalization of Native Antibodies.

  2019cites this paper
• Covalently binding mucoadhesive polymers: N‐hydroxysuccinimide grafted polyacrylates

  2019cites this paper
• Immobilized Peptide Asparaginyl Ligases Enhance Stability and Facilitate Macrocyclization and Site-specific Ligation.

  2019cites this paper
• Linker-free antibody conjugation for sensitive hydrogel microparticle-based multiplex immunoassay.

  2019cites this paper
• Carbodiimide-mediated immobilization of acidic biomolecules on reversed-charge zwitterionic sensor chip surfaces

  2018cites this paper
• Voltammetric determination of N-hydroxysuccinimide at conductive diamond electrodes.

  2018cites this paper
• Entwicklung und Anwendungen eines SPR-Biosensor-Assays für Moenomycine und andere Glykosyltransferase-Inhibitoren

  2018cites this paper
• A linear epitope coupled to DsRed provides an affinity ligand for the capture of monoclonal antibodies.

  2018cites this paper
• Surfactant-free Colloidal Particles with Specific Binding Affinity

  2017cites this paper
• Chemically-defined camelid antibody bioconjugate for the magnetic resonance imaging of Alzheimer's disease

  2017cites this paper
• A comparative study of short chain and long chain mercapto acids used in biosensor fabrication: A VEGF-R1-based immunosensor as a model system

  2016cites this paper
• Development of Molecularly Imprinted Polymer‐based Microcantilever Sensor System

  2016cites this paper
• Covalent immobilization of antibodies for the preparation of immunoaffinity chromatographic supports

  2016cites this paper
• Systematic Investigation of EDC/sNHS-Mediated Bioconjugation Reactions for Carboxylated Peptide Substrates.

  2016cites this paper
• Targeted Modulation of Tropoelastin Structure and Assembly

  2016cites this paper
• Interaction of Graphene Oxide with Bacterial Cell Membranes: Insights from Force Spectroscopy

  2015cites this paper
• Quantification of N-hydroxysuccinimide and N-hydroxysulfosuccinimide by hydrophilic interaction chromatography (HILIC)

  2015cites this paper
• Studying Biomolecular Structures and Their Interaction Using Atomic Force Microscopy

  2015cites this paper
• Developments and recent advancements in the field of endogenous amino acid selective bond forming reactions for bioconjugation.

  2015cites this paper
• CARACTERIZAÇÃO DE SUBSTRATOS PLÁSTICOS

  2014cites this paper
• Development of new bioselective ligation reactions

  2014cites this paper
• Inhibition of metastatic tumor growth and metastasis via targeting metastatic breast cancer by chlorotoxin-modified liposomes.

  2014cites this paper
• TOF-SIMS surface analysis of L-Tryptophan self assembled monolayer

  2014cites this paper
• Molecular Modification of Protein A to Improve the Elution pH and Alkali Resistance in Affinity Chromatography

  2014cites this paper
• Optomechanische Untersuchungen an Biomolekülen mittels Rastersondenmikroskopie

  2014cites this paper
• Bionanoconjugation for proteomics applications - An overview.

  2014cites this paper
• Gas Phase Reactivity of Carboxylates with N-Hydroxysuccinimide Esters

  2014cites this paper
• COMPARATIVE DISC ELECTROPHORETIC PROTEIN ANALYSES AND SEROLOGICAL RELATIONSHIPS OF SELECTED SPECIES OF Meloidogyne AND SOME HOST PLANTS

  2014cites this paper
• Application of catalyst-free click reactions in attaching affinity molecules to tips of atomic force microscopy for detection of protein biomarkers.

  2013cites this paper
• Dye-protein interactions : protein staining and dye-IgY, dye-dextran-IgY complexes for antigen detection.

  2013cites this paper
• Nano-agglomerated capillary polymer monoliths for applications in micro-catalysis and separation science

  2013cites this paper
• Ni–NTA-COATED NANOWIRE MATERIALS FOR PROTEIN ENRICHMENT AND THE APPLICATION IN A MEDICAL DEVICE USED FOR BLOOD GLUCOSE DEGRADATION

  2013cites this paper
• A new isoluminol reagent for chemiluminescence labeling of proteins

  2013cites this paper
• Functionalization of quartz crystal microbalances with liposomes containing the N-hydroxysuccinimide ester of palmitic acid.

  2013cites this paper
• Comparison of activated chromatography resins for protein immobilization.

  2013cites this paper
• Functional and Structural Characterisation of Mutant Tropoelastin Constructs

  2013cites this paper
• Bioactive Self-Assembled Monolayer Gradients

  2012cites this paper
• Monodisperse, "highly" positively charged protein polymer drag-tags generated in an intein-mediated purification system used in free-solution electrophoretic separations of DNA.

  2012cites this paper
• Quantification of proteins on gold nanoparticles by combining MALDI-TOF MS and proteolysis

  2012cites this paper
• Systematic study of fluorescein-functionalized macrophotoinitiators for colorimetric bioassays.

  2012cites this paper
• Separation of Sensitized and Non-Sensitized RBCs: Sephadex-Based Cell-Affinity Adsorbents

  2012cites this paper
• Construction of ribosome display library based on lipocalin scaffold and screening anticalins with specificity for estradiol.

  2012cites this paper
• Protein isolation using affinity chromatography

  2012cites this paper
• General Approaches for Chemical Cross-Linking

  2011cites this paper
• Affinity chromatography.

  2011cites this paper
• Biologically modified hydrogels for chemical and biochemical analysis.

  2011cites this paper
• Multifunctional Cross-Linking Reagents

  2011cites this paper
• Activity and stability of immobilized carbonic anhydrase for promoting CO2 absorption into a carbonate solution for post-combustion CO2 capture.

  2011cites this paper
• Completely monodisperse, highly repetitive proteins for bioconjugate capillary electrophoresis: development and characterization.

  2011cites this paper
• Micro-CT enables microlocalisation and quantification of Her2-targeted gold nanoparticles within tumour regions.

  2011cites this paper
• Immobilization and characterization of benzoylformate decarboxylase from Pseudomonas putida on spherical silica carrier

  2011cites this paper
• Surface Sensitization Techniques and Recognition Receptors Immobilization on Biosensors and Microarrays

  2010influential citation
• Proteomic Analysis of Glutathione Transferases from Lucilia Cuprina

  2010cites this paper
• The Synthesis and Characteristics of a Novel Hydrogel Based on Linear Polyethylenimine

  2010cites this paper
• False labelling due to quenching failure of N‐hydroxy‐succinimide–ester‐coupled dyes

  2010cites this paper
• Role of arginine in chemical cross-linking with N-hydroxysuccinimide esters.

  2010cites this paper
• Multiphoton-absorption-induced-luminescence (MAIL) imaging of tumor-targeted gold nanoparticles.

  2010cites this paper
• Using "click" chemistry to prepare SAM substrates to study stem cell adhesion.

  2009cites this paper
• Comparative electrochemical behavior of glucose oxidase covalently immobilized on mono-, di- and tetra-carboxylic acid functional Au-thiol SAMs via anhydride-derivatization route

  2009cites this paper
• Polyolefin fibers with chemically fixed active ester for the solid phase synthesis of an amide derivative

  2009cites this paper
• Chemical cross-linking with NHS esters: a systematic study on amino acid reactivities.

  2009cites this paper
• Solid-phase active site inhibitors of proteinases.

  2009cites this paper
• Multivalent Binding of p53 to the STAGA Complex Mediates Coactivator Recruitment after UV Damage

  2008cites this paper
• Reactive landing of peptide ions on self-assembled monolayer surfaces: an alternative approach for covalent immobilization of peptides on surfaces.

  2008cites this paper
• Magnetic proteinase K reactor as a new tool for reproducible limited protein digestion.

  2008cites this paper
• Vinylogous ureas as a novel class of inhibitors of reverse transcriptase-associated ribonuclease H activity.

  2008cites this paper
• Isolation and purification of Clostridium perfringens enterotoxin by affinity chromatography.

  2008cites this paper
• Fluorescence Spectroscopy: Applications in Chemical Biology

  2008cites this paper
• 2.12 – How and Why to Apply the Latest Technology*

  2007cites this paper
• Purple membranes as microscaled nanopatterned biosubstrates for reversible attachment of biocomponents.

  2007cites this paper