IDENTIFICATION OF A MAJOR BASIC PROTEIN IN GUINEA PIG EOSINOPHIL GRANULES

Elucidation of the functions of the eosinophil might be accomplished by analysis of the granule constituents. We have purified eosinophils (93% or greater) from the peritoneal cavity of the guinea pig and have investigated a variety of methods to disrupt cells and liberate intact granules. Lysis in 0.34 M sucrose gave the best yield of granules and these had the characteristic morphology of eosinophil granules when examined by electron microscopy. Granules were solubilized by a variety of treatments and the solutions analyzed by polyacrylamide electrophoresis at pH 3 in 6 M urea. Comparison of the electrophoretic patterns of solubilized eosinophil and neutrophil granules revealed a difference: a major portion (53±3%; x ±1 SE) of the protein from the eosinophil granule migrated as a single component. This major band protein has a molecular weight between 6,000 and 12,000 daltons and a pI of 10 or greater. Analysis of eosinophil granule constituents on Sephadex G-50 revealed two main peaks; peak 1 possessed peroxidase activity and peak 2 contained the major band protein. These studies indicate that eosinophil granules contain a cationic protein of low molecular weight which lacks peroxidase activity and which accounts for greater than 50% of granule protein.

• Publication year

  1973

• Venue

  Journal of Experimental Medicine

• Publication date

  1973-06-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.137.6.1459PMID 4350835PMCID 2139342
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Guinea pig heterophil and eosinophil peroxidase.

  1972cited by this paper
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  1971cited by this paper
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  1969cited by this paper
• The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

  1969cited by this paper
• Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels.

  1969cited by this paper
• High resolution acrylamide gel electrophoresis of histones.

  1969cited by this paper
• ISOLATION AND CHARACTERIZATION OF PERMEABILITY FACTORS FROM RABBIT NEUTROPHILS

  1968cited by this paper
• ARGININE-RICH PROTEINS OF POLYMORPHONUCLEAR LEUKOCYTE LYSOSOMES

  1968cited by this paper
• MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES

  1968cited by this paper
• Cationic Proteins of Polymorphonuclear Leukocyte Lysosomes II. Composition, Properties, and Mechanism of Antibacterial Action

  1966cited by this paper
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  1966cited by this paper
• MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES

  1966cited by this paper
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  1966cited by this paper
• Neutrophil and Eosinophil Leucocytes

  1965cited by this paper
• PRODUCTION OF INFLAMMATORY CHANGES IN THE MICROCIRCULATION BY CATIONIC PROTEINS EXTRACTED FROM LYSOSOMES

  1964cited by this paper
• ISOLATION OF GRANULES FROM EOSINOPHIL LEUCOCYTES AND STUDY OF THEIR ENZYME CONTENT

  1963influential reference
• Disk Electrophoresis of Basic Proteins and Peptides on Polyacrylamide Gels

  1962cited by this paper
• THE ISOLATION AND PROPERTIES OF THE SPECIFIC CYTOPLASMIC GRANULES OF RABBIT POLYMORPHONUCLEAR LEUCOCYTES

  1960cited by this paper
• Studies in experimental eosinophilia. I. Repeated quantitation of peritoneal eosinophilia in guinea pigs by a method of peritoneal lavage.

  1960cited by this paper
• An electron microscopic study of red bone marrow.

  1956cited by this paper
• The properties of the isolated granules from blood eosinophiles.

  1953cited by this paper
• On the presence of an antihistaminicum in isolated eosinophilic granulocytes.

  1952cited by this paper

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• Eosinophils.

  2014cites this paper
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  2014cites this paper
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  2012cites this paper
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• Dissection of the posterior inferior cerebellar artery in the hypereosinophilic syndrome

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• [Role of eosinophils in allergic inflammation].

  2006cites this paper
• [The role of eosinophils in allergic inflammation].

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• Effects of xanthine derivatives on phosphatidylcholine secretion in rat type II pneumocytes in the presence of activated eosinophils.

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• [Peripheral blood eosinophils and serum level of eosinophil cationic protein in asthmatics].

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• Eosinophils in allergic diseases: immunopharmacological regulation.

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