Identification of neoblast- and regeneration-specific miRNAs in the planarian Schmidtea mediterranea

The remarkable regenerative capacity of the fresh water planarian Schmidtea mediterranea is useful for studying stem cell identity and function. Several novel miRNAs have recently been identified in this organism, but their roles in neoblasts and during regeneration are unknown. Here, the authors use next-generation sequencing to identify miRNAs that are enriched in neoblast subpopulations and in regenerating tissue at different time points during regeneration. The findings provide new insight into the identities of the miRNAs that support planarian regeneration and neoblast function.

• Publication year

  2013

• Venue

  RNA: A publication of the RNA Society

• Publication date

  2013-10-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1261/rna.038653.113PMID 23974438PMCID 3854530
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2013cited by this paper
• The cell biology of regeneration

  2012cited by this paper
• Argonaute-2 regulates the proliferation of adult stem cells in planarian

  2012cited by this paper
• Evolution and function of the extended miR-2 microRNA family

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• Defining the molecular profile of planarian pluripotent stem cells using a combinatorial RNA-seq, RNA interference and irradiation approach

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  2012cited by this paper
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  2011cited by this paper
• Constitutive gene expression and the specification of tissue identity in adult planarian biology.

  2011cited by this paper
• Planarian stem cells: a simple paradigm for regeneration.

  2011cited by this paper
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  2011cited by this paper
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• Transcriptome profiling and digital gene expression by deep-sequencing in normal/regenerative tissues of planarian Dugesia japonica.

  2011influential reference
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  2011cited by this paper
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• High-resolution profiling and discovery of planarian small RNAs

  2009influential reference
• edgeR: a Bioconductor package for differential expression analysis of digital gene expression data

  2009cited by this paper
• Formaldehyde‐based whole‐mount in situ hybridization method for planarians

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  2009influential reference
• Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea.

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  2008cited by this paper
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  2008cited by this paper
• Isolation of planarian X‐ray‐sensitive stem cells by fluorescence‐activated cell sorting

  2006cited by this paper
• MicroRNAs from the Planarian Schmidtea mediterranea: a model system for stem cell biology.

  2006cited by this paper
• Real-time quantification of microRNAs by stem–loop RT–PCR

  2005cited by this paper
• Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes.

  2005cited by this paper
• Open source clustering software.

  2004cited by this paper
• Intensified Cloning Efforts Have Revealed Numer

  2004cited by this paper
• Java Treeview - extensible visualization of microarray data

  2004cited by this paper
• A comparative.

  1950cited by this paper

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