Intraflagellar Transport: The Eyes Have It

Unlike most organelles, which are surrounded by cytoplasm, the flagellum protrudes from the cell surface extending tens or even hundreds of microns into the external medium. This elongated organelle must import all the macromolecules required for its assembly, maintenance, and function including >

• Publication year

  1999

• Venue

  Journal of Cell Biology

• Publication date

  1999-02-08

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.144.3.385PMID 9971734PMCID 2132910
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cytoplasmic dynein heavy chain 1b is required for flagellar assembly in Chlamydomonas.

  1999cited by this paper
• The DHC1b (DHC2) Isoform of Cytoplasmic Dynein Is Required for Flagellar Assembly

  1999cited by this paper
• Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein.

  1998influential reference
• Distinct Mutants of Retrograde Intraflagellar Transport (IFT) Share Similar Morphological and Molecular Defects

  1998cited by this paper
• A Dynein Light Chain Is Essential for the Retrograde Particle Movement of Intraflagellar Transport (IFT)

  1998cited by this paper
• Identification of the t Complex–encoded Cytoplasmic Dynein Light Chain Tctex1 in Inner Arm I1 Supports the Involvement of Flagellar Dyneins in Meiotic Drive

  1998cited by this paper
• Three flagellar motilities in Chlamydomonas unrelated to flagellar beating. Video supplement.

  1998cited by this paper
• Chlamydomonas Kinesin-II–dependent Intraflagellar Transport (IFT): IFT Particles Contain Proteins Required for Ciliary Assembly in Caenorhabditis elegans Sensory Neurons

  1998influential reference
• The role of preassembled cytoplasmic complexes in assembly of flagellar dynein subunits.

  1998cited by this paper
• Heterotrimeric Kinesin-II Is Required for the Assembly of Motile 9+2 Ciliary Axonemes on Sea Urchin Embryos

  1997cited by this paper
• mRNAs for Microtubule Proteins Are Specifically Colocalized during the Sequential Formation of Basal Body, Flagella, and Cytoskeletal Microtubules in the Differentiation of Naegleria gruberi

  1997cited by this paper
• Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella.

  1997cited by this paper
• Localization of kinesin superfamily proteins to the connecting cilium of fish photoreceptors.

  1996cited by this paper
• The kinesin-homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal bodies and centrioles.

  1996cited by this paper
• Inner dynein arms but not outer dynein arms require the activity of kinesin homologue protein KHP1(FLA10) to reach the distal part of flagella in Chlamydomonas

  1996cited by this paper
• A novel cytoplasmic dynein heavy chain: expression of DHC1b in mammalian ciliated epithelial cells.

  1996cited by this paper
• Kinesin-II, a membrane traffic motor in axons, axonemes, and spindles

  1996cited by this paper
• Brain Cytoplasmic and Flagellar Outer Arm Dyneins Share a Highly Conserved Mr 8,000 Light Chain*

  1996cited by this paper
• Flagellar assembly in two hundred and fifty easy-to-follow steps.

  1995cited by this paper
• The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane

  1995cited by this paper
• Exclusive expression of C. elegans osm-3 kinesin gene in chemosensory neurons open to the external environment.

  1995cited by this paper
• The Chlamydomonas FLA10 gene encodes a novel kinesin-homologous protein

  1994cited by this paper
• Localization of a kinesin-related protein to the central pair apparatus of the Chlamydomonas reinhardtii flagellum.

  1994cited by this paper
• A new kinesin-like protein (Klp1) localized to a single microtubule of the Chlamydomonas flagellum

  1994cited by this paper
• Kinesin-related proteins in eukaryotic flagella.

  1994cited by this paper
• 22S axonemal dynein is preassembled and functional prior to being transported to and attached on the axonemes.

  1994cited by this paper
• Phylogeny and expression of axonemal and cytoplasmic dynein genes in sea urchins.

  1994cited by this paper
• A motility in the eukaryotic flagellum unrelated to flagellar beating.

  1993influential reference
• C. elegans osm-3 gene mediating osmotic avoidance behaviour encodes a kinesin-like protein.

  1993cited by this paper
• Directed movements of ciliary and flagellar membrane components: A review

  1992cited by this paper
• Polarity of flagellar assembly in Chlamydomonas

  1992cited by this paper
• Genetic interactions at the FLA10 locus: suppressors and synthetic phenotypes that affect the cell cycle and flagellar function in Chlamydomonas reinhardtii.

  1991cited by this paper
• Cell body and flagellar agglutinins in Chlamydomonas reinhardtii: the cell body plasma membrane is a reservoir for agglutinins whose migration to the flagella is regulated by a functional barrier

  1990cited by this paper
• Mutant sensory cilia in the nematode Caenorhabditis elegans.

  1986cited by this paper
• Temperature-Sensitive, Assembly-Defective Flagella Mutants of CHLAMYDOMONAS REINHARDTII.

  1982cited by this paper
• Axonemal adenosine triphosphatases from flagella of Chlamydomonas reinhardtii. Purification of two dyneins.

  1979cited by this paper
• Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii

  1977cited by this paper
• A human syndrome caused by immotile cilia.

  1976cited by this paper
• BALD-2: a mutation affecting the formation of doublet and triplet sets of microtubules in Chlamydomonas reinhardtii

  1975cited by this paper
• Cells and tissues by light and electron microscopy = Microscopie photonique et électronique de tissus et de cellules

  1970cited by this paper
• FLAGELLAR MOTION AND FINE STRUCTURE OF THE FLAGELLAR APPARATUS IN CHLAMYDOMONAS

  1967cited by this paper
• FLAGELLAR REGENERATION IN PROTOZOAN FLAGELLATES

  1967cited by this paper

• Unique transcriptomes of sensory and non-sensory neurons: insights from Splicing Regulatory States

  2024cites this paper
• Cooption of regulatory modules for tektin paralogs during ciliary band formation in a marine annelid larva.

  2023cites this paper
• Experimental and molecular support for Cfap70 as a causative gene of ‘multiple morphological abnormalities of the flagella’ with male infertility

  2023cites this paper
• Recent advances in primary cilia in bone metabolism

  2023cites this paper
• Proteomic analysis of Giardia lamblia and Trichomonas vaginalis flagella reveal unique post‐translational modifications in tubulin that provide clues to regulation of their motilities

  2021cites this paper
• Biochemistry and physiology of zebrafish photoreceptors

  2021cites this paper
• Mouse Models of Inherited Retinal Degeneration with Photoreceptor Cell Loss

  2020cites this paper
• Physiological role of actin regulation in male fertility: Insight into actin capping proteins in spermatogenic cells

  2020cites this paper
• Rapid estimation of cytosolic ATP concentration from the ciliary beating frequency in the green alga Chlamydomonas reinhardtii

  2020cites this paper
• Length regulation of multiple 1 flagella that self-assemble from a 2 shared pool of components 3

  2019influential citation
• Defining the layers of a sensory cilium with STORM and cryoelectron nanoscopy

  2019cites this paper
• The photoreceptor cilium and its diseases.

  2019cites this paper
• Structural but Not Functional Alterations in Cones in the Absence of the Retinal Disease Protein Retinitis Pigmentosa 2 (RP2) in a Cone-Only Retina

  2019cites this paper
• IFT52 as a Novel Candidate for Ciliopathies Involving Retinal Degeneration.

  2018cites this paper
• Length regulation of multiple flagella that self-assemble from a shared pool of components

  2018cites this paper
• Intraflagellar transport: mechanisms of motor action, cooperation, and cargo delivery

  2017cites this paper
• Cilia - The sensory antennae in the eye.

  2017cites this paper
• Structural and Biochemical Characterization of Ciliary Targeting Complexes Reveals a Novel Dual Effector Bound Mechanism for the Rab11:GTP - FIP3 - Rabin8 Module

  2016cites this paper
• Studying Kinesin-2 regulation and coordination in vitro

  2016cites this paper
• MicroRNA-548a-5p promotes proliferation and inhibits apoptosis in hepatocellular carcinoma cells by targeting Tg737.

  2016cites this paper
• Polycystin-2 (PKD2), Eccentric (XNTA), and Meckelin (MKS3) in the Ciliated Model Organism Paramecium tetraurelia

  2015cites this paper
• Loss of retinitis pigmentosa 2 (RP2) protein affects cone photoreceptor sensory cilium elongation in mice

  2015cites this paper
• Unique among ciliopathies: primary ciliary dyskinesia, a motile cilia disorder

  2015cites this paper
• Three-dimensional architecture of murine rod cilium revealed by cryo-EM.

  2015cites this paper
• A marine phytoplankton (Prymnesium parvum) up-regulates ABC transporters and several other proteins to acclimatize with Fe-limitation.

  2014cites this paper
• Molecular complexes that direct rhodopsin transport to primary cilia.

  2014influential citation
• Cilia and ciliopathies: classic examples linking phenotype and genotype-an overview.

  2014cites this paper
• Rôle des microARN dans la différenciation de l'épithélium respiratoire humain : caractérisation de miR-449 comme acteur central de la multiciliogenèse conservé chez les vertébrés

  2013cites this paper
• Studies on recombination processes in two Chlamydomonas reinhardtii endogenous genes, NIT1 and ARG7.

  2013cites this paper
• Electron tomography of IFT particles.

  2013cites this paper
• Modeling the flexural rigidity of rod photoreceptors.

  2013cites this paper
• Generation and characterisation of monoclonal antibodies against cell cycle and cytokinesis regulators in Trypanosoma brucei

  2013cites this paper
• Stochastic mechano-chemical kinetics of molecular motors: A multidisciplinary enterprise from a physicist’s perspective

  2012cites this paper
• Kinesin-2 motors transport IFT-particles, dyneins and tubulin subunits to the tips of Caenorhabditis elegans sensory cilia: relevance to vision research?

  2012cites this paper
• Ciliary transition zone (TZ) proteins RPGR and CEP290: role in photoreceptor cilia and degenerative diseases

  2012cites this paper
• Structure and function of vertebrate cilia, towards a new taxonomy.

  2012cites this paper
• Regulation of microtubule dynamics by kinesins.

  2011cites this paper
• Accumulation of the Raf-1 Kinase Inhibitory Protein (Rkip) Is Associated with Cep290-mediated Photoreceptor Degeneration in Ciliopathies*

  2011cites this paper
• RPGR: Role in the photoreceptor cilium, human retinal disease, and gene therapy

  2011cites this paper
• Spotlight on childhood blindness.

  2011cites this paper
• Análisis genético molecular de la retinitis pigmetaria en dos familias que presentan diferentes modos de herencia mendeliana

  2011influential citation
• Delivery of Helper-dependent Adenoviral Vectors to the Subretinal Space of Mice

  2010cites this paper
• Multiprotein complexes of Retinitis Pigmentosa GTPase regulator (RPGR), a ciliary protein mutated in X-linked Retinitis Pigmentosa (XLRP).

  2010cites this paper
• The doublecortin gene family and disorders of neuronal structure.

  2010cites this paper
• Intraflagellar transport molecules in ciliary and nonciliary cells of the retina

  2010influential citation
• The retinitis pigmentosa protein RP2 interacts with polycystin 2 and regulates cilia-mediated vertebrate development.

  2010cites this paper
• Assay for in vitro budding of ciliary-targeted rhodopsin transport carriers.

  2009cites this paper
• RPGR-containing protein complexes in syndromic and non-syndromic retinal degeneration due to ciliary dysfunction

  2009cites this paper
• Photoreceptor IFT Complexes Containing Chaperones, Guanylyl Cyclase 1 and Rhodopsin

  2009cites this paper
• Cdc42- and IRSp53-dependent contractile filopodia tether presumptive lens and retina to coordinate epithelial invagination

  2009cites this paper
• Electron-tomographic analysis of intraflagellar transport particle trains in situ

  2009cites this paper
• Chapter 4 – Intraflagellar Transport

  2009cites this paper
• Intramanchette transport during primate spermiogenesis: expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis.

  2008cites this paper
• A ALYSIS OF PHOTORECEPTOR OUTER SEGME T MORPHOGE ESIS I ZEBRAFISH IFT57, IFT88 A D IFT172

  2008cites this paper
• Use of algae in the study of essential cell processes

  2008cites this paper
• Retinitis Pigmentosa GTPase Regulator (RPGR) protein isoforms in mammalian retina: insights into X-linked Retinitis Pigmentosa and associated ciliopathies.

  2008cites this paper
• Intraflagellar transport and the sensory outer segment of vertebrate photoreceptors

  2008cites this paper
• Intraflagellar transport motors in cilia: moving along the cell's antenna

  2008influential citation
• Complex genetic approaches to neurodegenerative diseases.

  2007cites this paper
• Insertional mutagenesis as a tool to study genes/functions in Chlamydomonas.

  2007cites this paper
• UNIVERSITA’ DEGLI STUDI DI TORINO

  2007cites this paper
• Complementation of the Chlamydomonas reinhardtii arg7-8 (arg2) point mutation by recombination with a truncated nonfunctional ARG7 gene.

  2007cites this paper
• Intraflagellar transport complex in Leishmania spp. In silico genome-wide screening and annotation of gene function.

  2007influential citation
• Untersuchungen zur physiologischen Rolle der CNGB1-Untereinheit im Riechprozess anhand einer CNGB1-defizienten Mauslinie

  2007cites this paper
• Disquinesia ciliar primaria. Reporte de caso

  2007cites this paper
• Proteomic analysis of rod photoreceptor outer segment and characterization of an aminophospholipid transporter-like ATPase

  2007cites this paper
• Untersuchungen zur physiologischen Rolle der CNGB1-Untereinheit im Riechprozess anhand einer CNGB1-defizienten Mauslinie

  2007cites this paper
• Cilia: tuning in to the cell's antenna.

  2006cites this paper
• Analysis of kinesin-2 function in photoreceptor cells using synchronous Cre-loxP knockout of Kif3a with RHO-Cre.

  2006cites this paper
• Kinesin-2 and photoreceptor cell death: requirement of motor subunits.

  2006cites this paper
• Genetic Analysis of the Cytoplasmic Dynein Subunit Families

  2006cites this paper
• Mutation of Polaris, an Intraflagellar Transport Protein, Shortens Neuronal Cilia

  2005cites this paper
• Mutations of Tubulin Glycylation Sites Reveal Cross-talk between the C Termini of α- and β-Tubulin and Affect the Ciliary Matrix in Tetrahymena*

  2005cites this paper
• The two cytoplasmic dynein-2 isoforms in Leishmania mexicana perform separate functions.

  2005cites this paper
• Mutations of tubulin glycylation sites reveal cross-talk between the C termini of alpha- and beta-tubulin and affect the ciliary matrix in Tetrahymena.

  2005cites this paper
• Cell Membrane Specializations as Revealed by the Freeze-Fracture Technique

  2005cites this paper
• Pathogenomics Analysis of Leishmania spp.: Flagellar Gene Families of Putative Virulence Factors

  2005cites this paper
• Actin‐Based Motility in the Net Slime Mould Labyrinthula: Evidence for the Role of Myosin in Gliding Movement

  2005cites this paper
• The Role of Dynein in Disease

  2004cites this paper
• Sequences required for the flagellar targeting of an integral membrane protein.

  2004cites this paper
• gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms.

  2004cites this paper
• Mechanosensory-defective, male-sterile unc mutants identify a novel basal body protein required for ciliogenesis in Drosophila

  2004cites this paper
• Genetics of photoreceptor development and function in zebrafish.

  2004cites this paper
• Intraflagellar transport genes are essential for differentiation and survival of vertebrate sensory neurons.

  2004cites this paper
• Follicle separation during Drosophila oogenesis requires the activity of the Kinesin II‐associated polypeptide Kap in germline cells

  2004influential citation
• Centrins, Potential Regulators of Transducin Translocation in Photoreceptor Cells

  2004cites this paper
• Polycystins and mechanosensation in renal and nodal cilia

  2004cites this paper
• Cell biology of normal and abnormal ciliogenesis in the ciliated epithelium.

  2004cites this paper
• Design and regulation of the AAA+ microtubule motor dynein.

  2004cites this paper
• Can heterologous gene expression shed (a torch) light on protein function?

  2004cites this paper
• Association of Pericentrin with the γ Tubulin Ring Complex: a Dissertation

  2004cites this paper
• LUÍS FERNANDO CARVALHO DE MENEZES Análise do padrão de expressão do produto de PKHD1, o gene mutado na doença renal policística autossômica recessiva

  2004cites this paper
• Photoreceptors and Intraflagellar Transport

  2004cites this paper
• Motors and Membrane Trafficking

  2004influential citation
• Hypoxia regulates assembly of cilia in suppressors of Tetrahymena lacking an IFT subunit gene

  2003cites this paper
• Drosophila KAP interacts with the kinesin II motor subunit KLP64D to assemble chordotonal sensory cilia, but not sperm tails.

  2003cites this paper
• Chlamydomonas DIP13 and human NA14: a new class of proteins associated with microtubule structures is involved in cell division

  2003cites this paper
• Photoreceptor intersegmental transport and retinal degeneration: a conserved pathway common to motile and sensory cilia.

  2003cites this paper
• Biology and Mechanism of Trypanosome Cell Motility

  2003cites this paper
• Characterization of Human Thioredoxin-like 2

  2003cites this paper