Multiplexed aberration measurement for deep tissue imaging in vivo

We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein–labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.

• Publication year

  2014

• Venue

  Nature Methods

• Publication date

  2014-07-25

• Fields of study

  Medicine, Physics, Engineering

• Identifiers
  DOI 10.1038/nmeth.3068PMID 25128976PMCID 4180771
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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