Reactive Oxygen Intermediates Are Involved in the Induction of CD95 Ligand mRNA Expression by Cytostatic Drugs in Hepatoma Cells*

Oxidative stress has been associated with the induction of programmed cell death. The CD95 ligand/receptor system is a specific mediator of apoptosis. We have used the model of drug-induced apoptosis to assess whether the CD95 ligand mRNA is induced by reactive oxygen intermediates. Treatment of HepG2 hepatoma cells with bleomycin induced the production of reactive oxygen intermediates and, as an additional parameter of oxidative stress, resulted in glutathione (GSH) depletion. In parallel, CD95 ligand mRNA expression was induced. In a similar fashion CD95 ligand mRNA expression increased after treatment with H2O2. Additional treatment with the antioxidant and GSH precursor N-acetylcysteine resulted in partial restoration of intracellular GSH levels and in reduced induction of CD95 ligand mRNA. Induction of CD95 ligand mRNA by bleomycin was further reduced by combined treatment withN-acetylcysteine and deferoxamine. These data suggest a direct role of oxygen radicals in the induction of the CD95 ligand.

• Publication year

  1997

• Venue

  Journal of Biological Chemistry

• Publication date

  1997-11-07

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1074/jbc.272.45.28191PMID 9353266
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Apoptosis by death factor.

  1997cited by this paper
• The CD95 system and the death of a lymphocyte.

  1997cited by this paper
• Mitochondrial control of apoptosis.

  1997cited by this paper
• Drug-induced apoptosis in hepatoma cells is mediated by the CD95 (APO-1/Fas) receptor/ligand system and involves activation of wild-type p53.

  1997cited by this paper
• FLICE is activated by association with the CD95 death‐inducing signaling complex (DISC)

  1997cited by this paper
• Role of reactive oxygen intermediates in TCR-induced death of T cell blasts and hybridomas.

  1996cited by this paper
• Human ICE/CED-3 protease nomenclature.

  1996cited by this paper
• Die and let live: eliminating dangerous lymphocytes.

  1996cited by this paper
• A license to kill.

  1996cited by this paper
• Lymphocyte apoptosis induced by CD95 (APO–1/Fas) ligand–expressing tumor cells — A mechanism of immune evasion?

  1996cited by this paper
• Reactive oxygen species and programmed cell death.

  1996cited by this paper
• Prevention of Islet Allograft Rejection with Engineered Myoblasts Expressing FasL in Mice

  1996cited by this paper
• Involvement of the CD95 (APO–1/Fas) receptor/ligand system in drug–induced apoptosis in leukemia cells

  1996cited by this paper
• The Fas death factor

  1995cited by this paper
• Thiol-mediated redox regulation of apoptosis. Possible roles of cellular thiols other than glutathione in T cell apoptosis.

  1995cited by this paper
• Impairment of endothelium-dependent dilation in rabbit renal arteries by oxidized lipoprotein(a). Role of oxygen-derived radicals.

  1995cited by this paper
• Expression of the Fas ligand in cells of T cell lineage.

  1995cited by this paper
• Autocrine T-cell suicide mediated by APO-1/(Fas/CD95)

  1995cited by this paper
• Apoptosis. Death of a T cell.

  1995cited by this paper
• Involvement of the CD95 (APO-1/Fas) receptor and ligand in liver damage

  1995cited by this paper
• APO-1 (CD95)-dependent and -independent antigen receptor-induced apoptosis in human T and B cell lines.

  1995cited by this paper
• Death of a T cell

  1995cited by this paper
• Regulation of cell surface APO‐1/Fas (CD95) ligand expression by metalloproteases

  1995cited by this paper
• Cellular defenses against damage from reactive oxygen species.

  1994cited by this paper
• Human Fas ligand: gene structure, chromosomal location and species specificity.

  1994cited by this paper
• Vitamin Ε Reduces Bleomycin-Induced Lung Fibrosis in Mice: Biochemical and Morphological Studies

  1993cited by this paper
• Polymerase chain reaction-based deletion screening of bleomycin induced 6-thioguanine-resistant mutants in Chinese hamster ovary cells: the effects of an inhibitor and a mimic of superoxide dismutase.

  1993cited by this paper
• Effects of an inhibitor and a mimic of superoxide dismutase on bleomycin mutagenesis in Chinese hamster ovary cells.

  1992cited by this paper
• A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry.

  1991cited by this paper
• Low concentrations of acid-soluble thiol (cysteine) in the blood plasma of HIV-1-infected patients.

  1989cited by this paper

• Cyanidin-3-rutinoside(C3R) attenuates free fatty acid-induced hepatic lipid accumulation in HepG2 cells

  2026cites this paper
• Impact of targeting the platelet-activating factor and its receptor in cancer treatment

  2025cites this paper
• Silibinin ameliorates abrin induced hepatotoxicity by attenuating oxidative stress, inflammation and inhibiting Fas pathway.

  2022cites this paper
• Protective prospects of eco-friendly synthesized selenium nanoparticles using Moringa oleifera or Moringa oleifera leaf extract against melamine induced nephrotoxicity in male rats.

  2021cites this paper
• sFasL—The Key to a Riddle: Immune Responses in Aging Lung and Disease

  2021cites this paper
• Bioactive peptide of Cicer arietinum L. induces apoptosis in human endometrial cancer via DNA fragmentation and cell cycle arrest

  2021cites this paper
• Antiproliferative and Oxidative Damage Protection Activities of Endophytic Fungi Aspergillus fumigatus and Chaetomium globosum from Moringa oleifera Lam.

  2021cites this paper
• Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents

  2018cites this paper
• Autoschizis: A Mode of Cell Death of Cancer Cells Induced by a Prooxidant TreatmentIn VitroandIn Vivo

  2018cites this paper
• Gene expression analysis of heat-shock proteins and redox regulators reveals combinatorial prognostic markers in carcinomas of the gastrointestinal tract

  2018cites this paper
• Vitamin K and its analogs: Potential avenues for prostate cancer management

  2017cites this paper
• Protective Effect of a Polyherbal Aqueous Extract Comprised of Nigella sativa (Seeds), Hemidesmus indicus (Roots), and Smilax glabra (Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes

  2017cites this paper
• Bioprobes at a Glance

  2017cites this paper
• Antioxidant Therapeutic Strategies for Cardiovascular Conditions Associated with Oxidative Stress

  2017cites this paper
• Pseudomonas Quinolone Signal Induces Oxidative Stress and Inhibits Heme Oxygenase-1 Expression in Lung Epithelial Cells

  2017cites this paper
• Vitis vinifera peel polyphenols stabilized gold nanoparticles induce cytotoxicity and apoptotic cell death in A431 skin cancer cell lines

  2017cites this paper
• Aniline Induces Oxidative Stress and Apoptosis of Primary Cultured Hepatocytes

  2016cites this paper
• Estudio de la Microbiota Intestinal en pacientes con Síndrome Metabólico y Enfermedad Hepática Grasa No Alcohólica

  2016cites this paper
• Hydrogen peroxide detection in viable and apoptotic tumor cells under action of cisplatin and bleomycin

  2016cites this paper
• Dihydromyricetin ameliorates oleic acid-induced lipid accumulation in L02 and HepG2 cells by inhibiting lipogenesis and oxidative stress.

  2016cites this paper
• Effects of the dexmedetomidine, midazolam, butorphanol, and atropine combination on plasma oxidative status and cardiorespiratory parameters in raccoon dogs (Nyctereutes procyonoides)

  2016cites this paper
• Biosynthesized Vitis vinifera seed gold nanoparticles induce apoptotic cell death in A431 skin cancer cells

  2016cites this paper
• Dose-Dependent Mutation Rates Determine Optimum Erlotinib Dosing Strategies for EGFR Mutant Non-Small Cell Lung Cancer Patients

  2015cites this paper
• Hugan Qingzhi Exerts Anti-Inflammatory Effects in a Rat Model of Nonalcoholic Fatty Liver Disease

  2015cites this paper
• Mechanisms and intervention strategies for alcohol and HIV-antiretroviral therapy-induced liver injury.

  2015cites this paper
• Evaluation of the cytotoxicity, cell-cycle arrest, and apoptotic induction by Euphorbia hirta in MCF-7 breast cancer cells

  2015cites this paper
• Epigenetically Enhanced Photodynamic Therapy (ePDT) is Superior to Conventional Photodynamic Therapy for Inducing Apoptosis in Cutaneous T‐Cell Lymphoma

  2015cites this paper
• Redox cycling of Cu(II) by 6-mercaptopurine leads to ROS generation and DNA breakage: possible mechanism of anticancer activity

  2015cites this paper
• Zinc Induces Apoptosis of Human Melanoma Cells, Increasing Reactive Oxygen Species, p53 and FAS Ligand.

  2015cites this paper
• Epigenetically Enhanced Photodynamic Therapy (ePDT) is Superior to Conventional Photodynamic Therapy for Inducing Apoptosis in Cutaneous T‐Cell Lymphoma

  2015cites this paper
• Oxidative / Antioxidant Status of Different Muscles of Fresh Pork Meat

  2015cites this paper
• Liver Function Breath Tests for Differentiation of Steatohepatitis From Simple Fatty Liver in Patients With Nonalcoholic Fatty Liver Disease

  2014cites this paper
• Evolving Concepts in the Pathogenesis of NASH: Beyond Steatosis and Inflammation

  2014cites this paper
• Zinc Status Alters Growth and Oxidative Stress Responses in Rat Hepatoma Cells

  2014cites this paper
• Therapeutics , Targets , and Chemical Biology Chemotherapeutic Agents Subvert Tumor Immunity by Generating Agonists of Platelet-Activating Factor

  2014cites this paper
• Chemotherapeutic agents subvert tumor immunity by generating agonists of platelet-activating factor.

  2014influential citation
• MODULATORY EFFECTS OF ASCORBIC ACID ON NEUROBEHAVIOURAL AND PHYSIOLOGICAL RESPONSES INDUCED BY PROPOFOL ANAESTHESIA IN GOATS

  2014cites this paper
• Induction of Non-Alcoholic Fatty Liver Disease in Mice

  2014cites this paper
• NPRL-Z-1, as a New Topoisomerase II Poison, Induces Cell Apoptosis and ROS Generation in Human Renal Carcinoma Cells

  2014cites this paper
• CR 108 , a novel vitamin K 3 derivative induces apoptosis and breast tumor inhibition by reactive oxygen species and mitochondrial dysfunction

  2013cites this paper
• Apoptosis of Human Leukemia Cells in Hyperthermia-induced α Role of Tumor Necrosis Factor

  2013cites this paper
• Cap1p attenuates the apoptosis of Candida albicans

  2013cites this paper
• Apoptosis in Cancer: Mechanisms, Deregulation, and Therapeutic Targeting

  2013cites this paper
• Estudo da eficiência de produção de peróxido de hidrogênio por naftoquinonas em associação com ácido ascórbico: aplicações como agentes citotóxicos em linhagem de células normal e tumoral

  2013cites this paper
• Independently of CD95 (APO-1/Fas) Signaling Liposomal ET-18-OCH3 Induces Cytochrome c-Mediated Apoptosis

  2013cites this paper
• Cancer-Induced Oxidative Stress and Pain

  2013cites this paper
• Requires an Enhanced Intracellular Tax-Mediated Apoptosis in Activated T Cells Human T Cell Leukemia Virus-I (HTLV-I)

  2013cites this paper
• CR108, a novel vitamin K3 derivative induces apoptosis and breast tumor inhibition by reactive oxygen species and mitochondrial dysfunction.

  2013cites this paper
• GS-2, a pyrazolo[1,5-a]indole derivative with inhibitory activity of topoisomerases, exerts its potent cytotoxic activity by ROS generation.

  2013cites this paper
• Some Findings on Apoptosis in Hepatocytes

  2012cites this paper
• Tumor invasion and oxidative stress: biomarkers and therapeutic strategies.

  2012cites this paper
• Central role of mitochondria in drug-induced liver injury

  2012cites this paper
• Chemotherapy-Induced Oxidative Stress in Nontargeted Normal Tissues

  2012cites this paper
• Anticancer activity of the iron facilitator LS081

  2011cites this paper
• Cell death and nonalcoholic steatohepatitis: where is ballooning relevant?

  2011cites this paper
• Leptin: the prototypic adipocytokine and its role in NAFLD.

  2010cites this paper
• Cellular Stress Responses: Cell Survival and Cell Death

  2010cites this paper
• Characterization of the interaction between the adaptor protein Nck and the protein kinase PKR

  2010cites this paper
• Vitamin E succinate (VES) inhibits cell growth and induces apoptosis by mitochondrial-derived ROS in SGC-7901 cells.

  2010cites this paper
• A novel compound modified from tanshinone inhibits tumor growth in vivo via activation of the intrinsic apoptotic pathway.

  2010cites this paper
• Beta-catenin in liver: A matter of life and death

  2010cites this paper
• Lycopene and Chemotherapy Toxicity

  2010cites this paper
• Nitric oxide and redox regulation in the liver: Part I. General considerations and redox biology in hepatitis.

  2010cites this paper
• Hydrogen Peroxide:A review of a scientifically verifiable omnipresent ubiquitous essentiality of obligate, aerobic, carbon-based life forms

  2009cites this paper
• The meaning of the antioxidative system in different gynaecological illnesses – a literature review

  2009cites this paper
• Effect of vitamin C administration on neutrophil apoptosis in septic patients after abdominal surgery.

  2009cites this paper
• Cytotoxic, genotoxic and oxidative stress induced by 1,4-naphthoquinone in B16F1 melanoma tumor cells.

  2009cites this paper
• Oxidative Stress and Drug Resistance in Cancer

  2009cites this paper
• Cell apoptosis induced by delta-elemene in colorectal adenocarcinoma cells via a mitochondrial-mediated pathway.

  2009cites this paper
• Adipokines in Nonalcoholic Steatohepatitis: From Pathogenesis to Implications in Diagnosis and Therapy

  2009cites this paper
• Alveolar epithelial and endothelial cell apoptosis in emphysema: What we know and what we need to know

  2008cites this paper
• Nonalcoholic fatty liver disease: from clinical recognition to treatment

  2008cites this paper
• Cell death signal by glycine- and proline-rich plant glycoprotein is transferred from cytochrome c and nuclear factor kappa B to caspase 3 in Hep3B cells.

  2008cites this paper
• Influence of therapy on the antioxidant status in patients with melanoma

  2008cites this paper
• , Jide Wang , Death in Human Gastric Epithelial Cells Oxidative Stress and Programmed Cell Infection Induces Helicobacter pylori

  2007cites this paper
• NASH - Diagnostik und Therapie: eine Übersicht

  2007cites this paper
• Helicobacter pylori infection induces oxidative stress and programmed cell death 2 in human gastric epithelial cells

  2007cites this paper
• Helicobacter pylori Infection Induces Oxidative Stress and Programmed Cell Death in Human Gastric Epithelial Cells

  2007cites this paper
• Innate immune response to double‐stranded RNA in biliary epithelial cells is associated with the pathogenesis of biliary atresia

  2007cites this paper
• Effect of the spin-labelled 1-ethyl-1-nitrosourea on CCNU-induced oxidative liver injury.

  2007cites this paper
• Effects of tiletamine-zolazepam anaesthesia on plasma antioxidative status and some haematological parameters in sheep.

  2007cites this paper
• Collateral damage in cancer chemotherapy: oxidative stress in nontargeted tissues.

  2007cites this paper
• Breaking chemoresistance and radioresistance with [213Bi]anti-CD45 antibodies in leukemia cells.

  2007cites this paper
• Bioconjugation of Oligonucleotides for Treating Liver Fibrosis

  2007cites this paper
• Mitochondrial-derived ROS in edelfosine-induced apoptosis in yeasts and tumor cells

  2007cites this paper
• Mechanisms inactivating the gene for E-cadherin in sporadic gastric carcinomas.

  2006cites this paper
• Hydrogen Peroxide Upregulates TNF-Related Apoptosis-Inducing Ligand (TRAIL) Expression in Human Astroglial Cells, and Augments Apoptosis of T Cells

  2006cites this paper
• Intracellular Hydrogen Peroxide Production Is an Upstream Event in Apoptosis Induced by Down-Regulation of Casein Kinase 2 in Prostate Cancer Cells

  2006cites this paper
• Effect of delta-elemene on Hela cell lines by apoptosis induction.

  2006cites this paper
• The Effect of δ-Elemene on Hela Cell Lines by Apoptosis Induction

  2006cites this paper
• Dosage effects of resveratrol on ethanol-induced cell death in the human K562 cell line.

  2006cites this paper
• UNIVERSIDADE DE CAXIAS DO SUL PRÓ-REITORIA DE PÓS-GRADUAÇÃO E PESQUISA COORDENADORIA DE PÓS-GRADUAÇÃO

  2006cites this paper
• The Evolving Role of Leptin and Adiponectin in Chronic Liver Diseases

  2006cites this paper
• Inhibition of tumour metastasis by targeted delivery of antioxidant enzymes

  2006cites this paper
• Treatment of nonalcoholic fatty liver disease.

  2006cites this paper
• Apoptosis induced by glycoprotein (150-kDa) isolated from Solanum nigrum L. is not related to intracellular reactive oxygen species (ROS) in HCT-116 cells

  2006cites this paper
• Influence of antigen stimulation on the oxidative burst in inbred and outbreed rabbits

  2006cites this paper
• The In Vitro and In Vivo Antitumor Activity of Vitamin C: K3 Combinations Against Prostate Cancer

  2005cites this paper
• Synthesis, Antioxidant Properties and Antiproliferative Activities of Tetrameric Copper and Copper‐Zinc Metal Complexes of Catecholamine Schiff Base Ligand

  2005cites this paper
• Redox regulation of neutrophil apoptosis and the systemic inflammatory response syndrome.

  2005cites this paper