Structures of glycosylphosphatidylinositol membrane anchors from Saccharomyces cerevisiae.

Metabolic labeling studies suggest that Saccharomyces cerevisiae contains many glycoproteins that are anchored in the lipid bilayer by glycosylphosphatidylinositol membrane anchors. Membrane anchors were purified from a crude yeast membrane protein fraction and analyzed by two-dimensional 1H-1H NMR, fast atom bombardment-mass spectrometry, compositional and methylation linkage analyses, as well as chemical and enzymatic modifications. The yeast glycosylphosphatidylinositol anchors consist of the following structures: ethanolamine-PO4-6(R-2)Man alpha 1-2Man alpha 1-6Man alpha 1-4Glc-NH2 alpha 1-6myo-inositol-1-PO4-lipid, where R is mainly Man alpha 1- (80%) with some Man alpha 1-2Man alpha 1- (15%) and Man alpha 1-3Man alpha 1- (5%). The core region of the yeast anchors (ethanolamine-PO4-6Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcNH2 alpha 1-6myo-inositol-1-PO4) is identical to the conserved core region found in glycosylphosphatidylinositol anchors from protozoa and mammals. The lipid moieties of the total yeast glycosylphosphatidylinositol anchors are mainly ceramides, consisting mostly of C18:0 phytosphingosine and C26:0 fatty acid. However, the lipid moiety of the glycosylphosphatidylinositol anchor of the purified ggp125 protein is a lyso- or diacylglycerol, containing C26:0 fatty acids. This suggests that yeast adds different lipid components to the glycosylphosphatidylinositol anchors of different proteins.

• Publication year

  1993

• Venue

  Journal of Biological Chemistry

• Publication date

  1993-12-15

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(19)74324-5PMID 8253761
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Glycan components in the glycoinositol phospholipid anchor of human erythrocyte acetylcholinesterase. Novel fragments produced by trifluoroacetic acid.

  1992cited by this paper
• Glycosylinositol phospholipid anchors of the scrapie and cellular prion proteins contain sialic acid.

  1992cited by this paper
• Site of palmitoylation of a phospholipase C-resistant glycosylphosphatidylinositol membrane anchor.

  1992cited by this paper
• Two different types of lipid moieties are present in glycophosphoinositol‐anchored membrane proteins of Saccharomyces cerevisiae.

  1992cited by this paper
• Purification, biosynthesis and cellular localization of a major 125-kDa glycophosphatidylinositol-anchored membrane glycoprotein of Saccharomyces cerevisiae.

  1991cited by this paper
• Glycophospholipid membrane anchor attachment. Molecular analysis of the cleavage/attachment site.

  1991cited by this paper
• Isolation and deduced amino acid sequence of the gene encoding gp115, a yeast glycophospholipid-anchored protein containing a serine-rich region.

  1991cited by this paper
• Developmental variation of glycosylphosphatidylinositol membrane anchors in Trypanosoma brucei. Identification of a candidate biosynthetic precursor of the glycosylphosphatidylinositol anchor of the major procyclic stage surface glycoprotein.

  1991cited by this paper
• Structure, biosynthesis, and function of glycosylphosphatidylinositols.

  1990cited by this paper
• Fatty acid remodeling: a novel reaction sequence in the biosynthesis of trypanosome glycosyl phosphatidylinositol membrane anchors.

  1990cited by this paper
• Glycolipid anchoring of plasma membrane proteins.

  1990cited by this paper
• Myoinositol gets incorporated into numerous membrane glycoproteins of Saccharomyces cerevisiae; incorporation is dependent on phosphomannomutase (sec53).

  1990cited by this paper
• Structure of the glycosyl-phosphatidylinositol membrane anchor of the Leishmania major promastigote surface protease.

  1990cited by this paper
• Molecular species analysis of the glycosylphosphatidylinositol anchor of Torpedo marmorata acetylcholinesterase.

  1990cited by this paper
• Structural basis for variations in the sensitivity of human decay accelerating factor to phosphatidylinositol-specific phospholipase C cleavage.

  1990cited by this paper
• Selectivity of the cleavage/attachment site of phosphatidylinositol-glycan-anchored membrane proteins determined by site-specific mutagenesis at Asp-484 of placental alkaline phosphatase.

  1990cited by this paper
• The cell cycle modulated glycoprotein GP115 is one of the major yeast proteins containing glycosylphosphatidylinositol.

  1990cited by this paper
• Biosynthesis of glycosyl phosphatidylinositol membrane anchors.

  1990cited by this paper
• Structures of the glycoinositolphospholipids from Leishmania major. A family of novel galactofuranose-containing glycolipids.

  1990cited by this paper
• Dolichol phosphate mannose synthase is required in vivo for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and N glycosylation of protein in Saccharomyces cerevisiae

  1990cited by this paper
• The glycosyl-phosphatidylinositol anchor of membrane proteins.

  1989cited by this paper
• The contact site A glycoprotein of Dictyostelium discoideum carries a phospholipid anchor of a novel type.

  1989cited by this paper
• A family of glycoinositol phospholipids from Leishmania major. Isolation, characterization, and antigenicity.

  1989cited by this paper
• A human membrane-associated folate binding protein is anchored by a glycosyl-phosphatidylinositol tail.

  1989cited by this paper
• A major 125‐kd membrane glycoprotein of Saccharomyces cerevisiae is attached to the lipid bilayer through an inositol‐containing phospholipid.

  1988cited by this paper
• Glycosyl-phosphatidylinositol moiety that anchors Trypanosoma brucei variant surface glycoprotein to the membrane.

  1988cited by this paper
• Complete structure of the glycosyl phosphatidylinositol membrane anchor of rat brain Thy-1 glycoprotein

  1988cited by this paper
• Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structures.

  1988cited by this paper
• Lipid analysis of the glycoinositol phospholipid membrane anchor of human erythrocyte acetylcholinesterase. Palmitoylation of inositol results in resistance to phosphatidylinositol-specific phospholipase C.

  1988cited by this paper
• Differences in the glycolipid membrane anchors of bovine and human erythrocyte acetylcholinesterases.

  1987cited by this paper
• F.A.B.-mass spectrometry of carbohydrates.

  1987cited by this paper
• The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases

  1986cited by this paper
• Hydrazinolysis-N-reacetylation of glycopeptides and glycoproteins. Model studies using 2-acetamido-1-N-(L-aspart-4-oyl)-2-deoxy-beta-D-glucopyranosy lamine.

  1985cited by this paper
• Trypanosoma brucei variant surface glycoprotein has a sn-1,2-dimyristyl glycerol membrane anchor at its COOH terminus.

  1985cited by this paper
• Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein.

  1985cited by this paper
• Analysis of oligosaccharides by gel filtration.

  1982cited by this paper
• Hydrazinolysis of asparagine-linked sugar chains to produce free oligosaccharides.

  1982cited by this paper
• Phase separation of integral membrane proteins in Triton X-114 solution.

  1981cited by this paper
• Inositol phosphorylceramide, a novel substance and the chief member of a major group of yeast sphingolipids containing a single inositol phosphate.

  1974cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• Characterization of temperature-sensitive alleles of the septation initiation network protein Mob1 in Schizosaccharomyces pombe

  2025cites this paper
• Generation and characterization of temperature-sensitive alleles encoding GPI anchored proteins Psu1 and Dfg502 in Schizosaccharomyces pombe

  2024cites this paper
• Identification of the glycosylphosphatidylinositol-specific phospholipase A2 (GPI-PLA2) that mediates GPI fatty acid remodeling in Trypanosoma brucei

  2023cites this paper
• Identification of the glycosylphosphatidylinositol-specific phospholipase A2 (GPI-PLA2) of GPI fatty acid remodelling in Trypanosoma brucei

  2023cites this paper
• Emerging connections between GPI-anchored proteins and their extracellular carriers in colorectal cancer

  2023cites this paper
• Lipids | Glycosylphosphatidylinositol Anchors

  2021cites this paper
• Diseases Associated With GPI Anchors

  2021cites this paper
• Mammalian GPI-anchor modifications and the enzymes involved.

  2020cites this paper
• Post-translational lipidation in extracellular vesicles: chemical mechanisms, biological functions and applications

  2020cites this paper
• Galactomannan Produced by Aspergillus fumigatus: An Update on the Structure, Biosynthesis and Biological Functions of an Emblematic Fungal Biomarker

  2020cites this paper
• Glycobiology of Yeast: Applications to Glycoprotein Expression and Remodeling

  2020cites this paper
• Protozoan parasites glycosylphosphatidylinositol anchors: structures, functions and trends for drug discovery.

  2019cites this paper
• ANA LUÍSA MALACO MOROTTI Synthesis of GPI anchor analogues to support the discovery of new molecular targets of Trypanosoma cruzi Síntese de análogos de âncora de GPI: uma contribuição para a descoberta de novos alvos moleculares de Trypanosoma cruzi

  2019cites this paper
• Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus

  2018cites this paper
• Generating anchors only to lose them: The unusual story of glycosylphosphatidylinositol anchor biosynthesis and remodeling in yeast and fungi

  2018cites this paper
• Sphingolipids from the human fungal pathogen Aspergillus fumigatus.

  2017cites this paper
• Mannoside recognition and degradation by bacteria

  2017cites this paper
• PER1, GUP1 and CWH43 of methylotrophic yeast Ogataea minuta are involved in cell wall integrity

  2017cites this paper
• Biosynthesis of GPI-anchored proteins: special emphasis on GPI lipid remodeling

  2016cites this paper
• Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts

  2016cites this paper
• SYNTHESIS OF GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORS

  2016cites this paper
• Aspergillus fumigatus Cap59-like protein A is involved in α1,3-mannosylation of GPI-anchors.

  2015cites this paper
• Expression And Functional Analysis Of Lipids And Glycolipids From The Mammal-Dwelling Stages Of Trypanosoma Cruzi

  2015cites this paper
• Cdc1 removes the ethanolamine phosphate of the first mannose of GPI anchors and thereby facilitates the integration of GPI proteins into the yeast cell wall

  2014cites this paper
• Complementation of Essential Yeast GPI Mannosyltransferase Mutations Suggests a Novel Specificity for Certain Trypanosoma and Plasmodium PigB Proteins

  2014cites this paper
• Glycosylphosphatidylinositols: Occurrence, Synthesis, and Properties

  2013cites this paper
• Membrane topology of yeast alkaline ceramidase YPC1.

  2013cites this paper
• Determination and physiological roles of the glycosylphosphatidylinositol lipid remodelling pathway in yeast

  2013influential citation
• New Long Chain Bases in Lipophosphonoglycan of Acanthamoeba Castellanii

  2013cites this paper
• Glycosylphosphatidylinositol: Protein Transamidase

  2013cites this paper
• Structural and biochemical characterisation of enzymes involved in mannan biosynthesis

  2013cites this paper
• Development And Optimization Of The First High Throughput In Vitro Fret Assay To Characterize The Saccharomyces Cerevisiae Gpi-T

  2013cites this paper
• Chemical biology of glycosylphosphatidylinositol anchors.

  2012cites this paper
• Biosynthesis of the Fungal Cell Wall Polysaccharide Galactomannan Requires Intraluminal GDP-mannose*

  2012cites this paper
• Chemische Biologie der Glycosylphosphatidylinosit‐Anker

  2012cites this paper
• Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

  2012influential citation
• Biosynthesis of the Fungal Cell Wall Polysaccharide Galactomannan Requires Intraluminal GDP-Mannose

  2012cites this paper
• A survey of Ley's reactivity tuning in oligosaccharide synthesis.

  2012cites this paper
• Some biomolecules and a partially O-acetylated exo-galactomannan containing β-Galf units from pathogenic Exophiala jeanselmei, having a pronounced immunogenic response.

  2011cites this paper
• Structural remodeling, trafficking and functions of glycosylphosphatidylinositol-anchored proteins.

  2011cites this paper
• Synthetic glycosylphosphatidylinositol (GPI) anchors: how these complex molecules have been made.

  2011cites this paper
• The Sphingolipid Biosynthetic Pathway Is a Potential Target for Chemotherapy against Chagas Disease

  2011cites this paper
• Regulation of phospholipid synthesis in the yeast Saccharomyces cerevisiae.

  2011cites this paper
• REGULATION OF YEAST CHOLINE KINASE BY ZINC DEPLETION AND THE YEAST PHOSPHATIDATE PHOSPHATASE BY ZINC DEPLETION AND GROWTH

  2010cites this paper
• Synthesis of a GPI anchor module suitable for protein post-translational modification.

  2010cites this paper
• Chemical synthesis, modification and mimicry of the GPI anchor

  2010cites this paper
• Synthesis of truncated analogues for studying the process of glycosyl phosphatidylinositol modification.

  2010cites this paper
• Lipidation of Peptides and Proteins

  2010cites this paper
• Multiple Functions as Lipase, Steryl Ester Hydrolase, Phospholipase, and Acyltransferase of Tgl4p from the Yeast Saccharomyces cerevisiae*

  2010cites this paper
• Structural remodeling of GPI anchors during biosynthesis and after attachment to proteins

  2010influential citation
• Yeast Cells Lacking All Known Ceramide Synthases Continue to Make Complex Sphingolipids and to Incorporate Ceramides into Glycosylphosphatidylinositol (GPI) Anchors*

  2010cites this paper
• Application of electrospray mass spectrometry to the structural determination of glycosylphosphatidylinositol membrane anchors

  2010cites this paper
• Chapter 13 Transport of GPI‐Anchored Proteins: Connections to Sphingolipid and Sterol Transport

  2009cites this paper
• Chapter 1 Overview of GPI Biosynthesis

  2009cites this paper
• Incorporation of ceramides into yeast GPI anchored proteins can be monitored in vitro

  2009influential citation
• Lipid remodeling of GPI-anchored proteins and its function.

  2008cites this paper
• Characterizing the interactions between GPI-anchored alkaline phosphatases and membrane domains by AFM

  2008cites this paper
• The Role of Rsp5 Ubiquitin Ligase in Regulation of Diverse Processes in Yeast Cells

  2008cites this paper
• Incorporation of Ceramides into Saccharomyces cerevisiae Glycosylphosphatidylinositol-Anchored Proteins Can Be Monitored In Vitro

  2008cites this paper
• The role of Rsp5 ubiquitin ligase in regulation of diverse processes in yeast cells.

  2008cites this paper
• Hemoglobinuria paroxística nocturna

  2008cites this paper
• [Paroxysmal nocturnal hemoglobinuria].

  2008cites this paper
• GPIs on a chip.

  2008cites this paper
• Regulation of the Saccharomyces cerevisiae CKI1-encoded Choline Kinase by Zinc Depletion*

  2008cites this paper
• Chemical Synthesis of Glycosylphosphatidylinositol (GPI) Anchors and GPI-Linked Structures

  2008cites this paper
• 4.21 – Diseases Associated with GPI Anchors

  2007cites this paper
• Multiple functions of inositolphosphorylceramides in the formation and intracellular transport of glycosylphosphatidylinositol-anchored proteins in yeast.

  2007cites this paper
• Thematic review series: Lipid Posttranslational Modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids Published, JLR Papers in Press, March 14, 2007.

  2007cites this paper
• Regulation of phospholipid synthesis in Saccharomyces cerevisiae by zinc depletion.

  2007cites this paper
• Biosynthesis and function of GPI proteins in the yeast Saccharomyces cerevisiae.

  2007influential citation
• CWH43 is required for the introduction of ceramides into GPI anchors in Saccharomyces cerevisiae

  2007cites this paper
• Yeast sphingolipids do not need to contain very long chain fatty acids.

  2007cites this paper
• Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid moiety of GPI anchors to ceramides.

  2007influential citation
• The Gup1 homologue of Trypanosoma brucei is a GPI glycosylphosphatidylinositol remodelase

  2007cites this paper
• GUP1 of Saccharomyces cerevisiae encodes an O-acyltransferase involved in remodeling of the GPI anchor.

  2006cites this paper
• Cell Wall Assembly in Saccharomyces cerevisiae

  2006cites this paper
• Regulation of the Saccharomyces cerevisiae EKI1-encoded Ethanolamine Kinase by Zinc Depletion*

  2006cites this paper
• Recent developments in the molecular, biochemical and functional characterization of GPI8 and the GPI-anchoring mechanism [Review]

  2006cites this paper
• Glycosyltransferases and their products: cryptococcal variations on fungal themes.

  2006cites this paper
• Ethanolaminephosphate Side Chain Added to Glycosylphosphatidylinositol (GPI) Anchor by Mcd4p Is Required for Ceramide Remodeling and Forward Transport of GPI Proteins from Endoplasmic Reticulum to Golgi*

  2006cites this paper
• PER1 is required for GPI-phospholipase A2 activity and involved in lipid remodeling of GPI-anchored proteins.

  2006cites this paper
• Isolation and characterization of glycosylphosphatidylinositol-anchored peptides by hydrophilic interaction chromatography and MALDI tandem mass spectrometry.

  2006cites this paper
• An efficient synthesis of D-ribo- and L-lyxo-phytosphingosine from D-tartaric acid

  2005cites this paper
• Glycosylphosphatidylinositol-anchored Fungal Polysaccharide in Aspergillus fumigatus*

  2005cites this paper
• Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly

  2005cites this paper
• Human Smp3p Adds a Fourth Mannose to Yeast and Human Glycosylphosphatidylinositol Precursors in Vivo*

  2004cites this paper
• Regulation of Phospholipid Synthesis in Saccharomyces cerevisiae by Zinc*

  2004cites this paper
• Sorting GPI-anchored proteins

  2004cites this paper
• Synthesis of two oligosaccharides, the GPI anchor glycans from S. cerevesiae and A. fumigatus.

  2004cites this paper
• In vitro biosynthesis of glycosylphosphatidylinositol in Aspergillus fumigatus.

  2004cites this paper
• Expression of Transglutaminase Substrate Activity on Candida albicans Germ Tubes through a Coiled, Disulfide-bonded N-terminal Domain of Hwp1 Requires C-terminal Glycosylphosphatidylinositol Modification*

  2004cites this paper
• Synthesis of l-lyxo-phytosphingosine and its 1-phosphonate analogue using a threitol acetal synthon.

  2004cites this paper
• Glycosylphosphatidylinositol (GPI) Proteins of Saccharomyces cerevisiae Contain Ethanolamine Phosphate Groups on the α1,4-linked Mannose of the GPI Anchor*

  2004cites this paper
• Deficiencies in the essential Smp3 mannosyltransferase block glycosylphosphatidylinositol assembly and lead to defects in growth and cell wall biogenesis in Candida albicans.

  2004influential citation
• Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi.

  2004cites this paper
• Formation and Remodeling of Inositolphosphoceramide during Differentiation of Trypanosoma cruzi from Trypomastigote to Amastigote

  2003cites this paper
• Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins.

  2003influential citation
• Genes for Glycosylphosphatidylinositol Toxin Biosynthesis in Plasmodium falciparum

  2002cites this paper
• Cloning and Expression of an Endo-1,6-β-D-glucanase Gene (neg1) from Neurospora crassa

  2002cites this paper
• Fate of Mammalian Golgi Sialyltransferases in Yeast

  2002cites this paper