A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY

A disadvantage of the lead strains used in electron microscopy is the amount of time required for their preparation. One of the more stable and reliable stains, commonly called the lead citrate stain or Reynolds' stain (Reynolds, 1963), is made by mixing lead nitrate and sodium citrate in distilled water, allowing time for lead citrate to form, then adding sodium hydroxide to raise the pH of the solution to 12. This communication reports the use of a commercially available lead citratO to eliminate the

• Publication year

  1965

• Venue

  Journal of Cell Biology

• Publication date

  1965-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.25.2.407PMID 14287192PMCID 2106628
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY

  1963cited by this paper

• Age- and Light-Dependent Changes in the Zebrafish Olfactory Epithelium

  2026cites this paper
• Neurodegeneration

  2025cites this paper
• Lysosome-dependent nutrient scavenging underlies stress adaptation during epithelial-to-mesenchymal transition

  2025cites this paper
• Exploring Antimycobacterial Potential: Safety Evaluation and Active Compound Isolation from Gymnopilus junonius

  2025cites this paper
• DNA damage response regulator ATR licenses PINK1-mediated mitophagy

  2025cites this paper
• Mitochondria dysfunction and increased expression of WNT5A and BNIP3 in tenocytes obtained from patients with tendinopathy

  2025cites this paper
• Optimization of Human STARD3 Cholesterol Transporter Expression for Enhancing Bovine P450scc-Mediated Steroid Biotransformation in E. coli.

  2025cites this paper
• Ectomycorrhizas in Lycopodiopsida: their first registry and arbuscular mycorrhiza in Phlegmariurus saururus (Huperziaceae).

  2025cites this paper
• Neurons, Muscles, and Venom: Identifying Drivers of Cephalopod Predation

  2025cites this paper
• ESCRT disruption provides evidence against trans-synaptic signaling via extracellular vesicles

  2024cites this paper
• Prominin-1 null Xenopus laevis develop subretinal drusenoid-like deposits, cone-rod dystrophy, and RPE atrophy

  2024cites this paper
• The Role of Gastric Lipase and Pepsin in Lipid Digestion of a Powder Infant Formula Using a Simulated Neonatal Gastric System

  2024cites this paper
• Transcriptome profiling of the posterior salivary glands of the cuttlefish Sepia officinalis from the Portuguese West coast

  2024cites this paper
• Purification of time-resolved insulin granules reveals proteomic and lipidomic changes during granule aging.

  2024cites this paper
• Cadmium and zinc accumulation and tolerance in two Egyptian cultivars (S53 and V120) of Helianthus annuus L. as potential phytoremediator

  2024cites this paper
• A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina

  2024cites this paper
• Extensive monolayer formation depends on a subpopulation of transplanted human iPSC-derived RPE cells

  2024cites this paper
• prominin-1-null Xenopus laevis develop subretinal drusenoid-like deposits, cone-rod dystrophy and RPE atrophy

  2024cites this paper
• Human iPSC 4R tauopathy model uncovers modifiers of tau propagation.

  2024cites this paper
• Updating the Knowledge on the Secretory Machinery of Hops (Humulus lupulus L., Cannabaceae)

  2024cites this paper
• Compartmentalization and synergy of osteoblasts drive bone formation in the regenerating fin

  2024cites this paper
• ESCRT disruption provides evidence against transsynaptic signaling functions for extracellular vesicles

  2024cites this paper
• Inceptor binds to and directs insulin towards lysosomal degradation in β cells

  2024cites this paper
• Automated quantification of photoreceptor outer segments in developing and degenerating retinas on microscopy images across scales

  2024cites this paper
• RNA binding protein SYNCRIP maintains proteostasis and self-renewal of hematopoietic stem and progenitor cells

  2023cites this paper
• Human iPSC 4R tauopathy model uncovers modifiers of tau propagation

  2023influential citation
• Transplantation of porcine adrenal spheroids for the treatment of adrenal insufficiency

  2023cites this paper
• Terebra steering in chalcidoid wasps

  2023cites this paper
• Compartmentalization and synergy of osteoblasts drive bone formation in the regenerating fin

  2023cites this paper
• Sustained CD28 costimulation is required for self-renewal and differentiation of TCF-1+ PD-1+ CD8 T cells

  2023cites this paper
• ESCRT disruption provides evidence against signaling functions for synaptic exosomes

  2023cites this paper
• Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility

  2023cites this paper
• Prolonged Activity Deprivation Causes Pre- and Postsynaptic Compensatory Plasticity at Neocortical Excitatory Synapses

  2023cites this paper
• Deletion of Tbc1d4/As160 abrogates cardiac glucose uptake and increases myocardial damage after ischemia/reperfusion

  2023cites this paper
• Insulin regulates human pancreatic endocrine cell differentiation in vitro

  2023cites this paper
• A vertebral skeletal stem cell lineage driving metastasis

  2023cites this paper
• Revisiting the formation of midgut epithelium in Zygentoma (Insecta) from a developmental study of the firebrat Thermobia domestica (Packard, 1873) (Lepismatidae).

  2023cites this paper
• The ultrastructure of spermatozoa of two species of Aegla (A. parana and A. quilombola) (Crustacea, Decapoda) endemic to Brazil.

  2023cites this paper
• Adaptative Strategies in Gymnocalycium Species (Cactaceae) and the Presence of Ectomycorrhizae Associated with Survival in Arid Environments

  2023cites this paper
• Altered expression of vesicular glutamate transporter-2 and cleaved caspase-3 in the locus coeruleus of nerve-injured rats

  2022cites this paper
• Aphelidium parallelum, sp. nov., a new aphelid parasitic on selenastracean green algae

  2022cites this paper
• Phenotype and Distribution of Immature Neurons in the Human Cerebral Cortex Layer II

  2022cites this paper
• Artificial Digestion of Polydisperse Copper Oxide Nanoparticles: Investigation of Effects on the Human In Vitro Intestinal Co-Culture Model Caco-2/HT29-MTX

  2022cites this paper
• Cyphellostereum ushimanum sp. nov. (Hygrophoraceae, Agaricales) described from Amami-Oshima Island (Kagoshima Prefecture, Ryukyu Islands), Japan, with ultrastructural observations of its Rhizonema photobiont filaments penetrated longitudinally by a central haustorium

  2022cites this paper
• SS-31 as a Mitochondrial Protectant in the Treatment of Tendinopathy

  2022cites this paper
• Evaluation of SS-31 as a Potential Strategy for Tendinopathy Treatment: An In Vitro Model

  2022cites this paper
• A Promising Use of Trimethyl Chitosan for Removing Microcystis aeruginosa in Water Treatment Processes

  2022cites this paper
• Polyphosphate accumulation and cell-surface properties by autochthonous bacteria from Argentinian Patagonia.

  2022cites this paper
• Cysteine induces mitochondrial reductive stress in glioblastoma through hydrogen peroxide production

  2021cites this paper
• Soy Protein Pressed Gels: Gelation Mechanism Affects the In Vitro Proteolysis and Bioaccessibility of Added Phenolic Acids

  2021cites this paper
• Metal-Pseudomonas veronii 2E Interactions as Strategies for Innovative Process Developments in Environmental Biotechnology

  2021cites this paper
• Chemical Treatments for Insect Cell Differentiation: The Effects of 20-Hydroxyecdysone and Veratridine on Cultured Spodoptera frugiperda (Sf21) Insect Cell Ultrastructure

  2021cites this paper
• Plasma Membrane-Localized SEM1 Protein Mediates Sugar Movement to Sink Rice Tissues.

  2021cites this paper
• Structural traits of leaf epidermis correspond to metal tolerance in Rumex acetosella populations growing on metal-contaminated soils

  2021cites this paper
• Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor niche

  2021cites this paper
• Revisiting staining of biological samples for electron microscopy: perspectives for recent research.

  2021cites this paper
• Systematic Transmission Electron Microscopy-Based Identification and 3D Reconstruction of Cellular Degradation Machinery

  2021cites this paper
• Amelioration potential of β-pinene on Cr(VI)-induced toxicity on morphology, physiology and ultrastructure of maize

  2021cites this paper
• Bacterial microcompartments for isethionate desulfonation in the taurine-degrading human-gut bacterium Bilophila wadsworthia

  2021cites this paper
• TITLE: Systematic Transmission Electron Microscopy-Based Identification of Cellular Degradation Machinery AUTHORS AND AFFILIATIONS:

  2021cites this paper
• Opposing functions for retromer and Rab11 in extracellular vesicle traffic at presynaptic terminals

  2021cites this paper
• A workflow to investigate the impacts of weathered multi-walled carbon nanotubes to the mud snail Lymnaea stagnalis

  2021cites this paper
• Tbx1, a 22q11.2-encoded gene, is a link between alterations in fimbria myelination and cognitive speed in mice

  2021cites this paper
• Mouse Retinal Organoid Growth and Maintenance in Longer-Term Culture

  2021cites this paper
• Mutations in EPHB4 cause human venous valve aplasia

  2021cites this paper
• Liver description in three neotropical anuran species: from anatomy to ultrastructure

  2021cites this paper
• β2-adrenergic receptor regulates ER-mitochondria contacts

  2021cites this paper
• Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from the Marine Annelid Eulalia sp. onto Ovarian Cancer Cells

  2021cites this paper
• The Structure of the Spinal Cord Ependymal Region in Adult Humans Is a Distinctive Trait among Mammals

  2021cites this paper
• ULTRASTRUCTURAL CHANGES IN ORIZA SATIVA, Echinochloa colonum AND Echinochloa crus-galli TREATED WITH FENOXAPROP-P-ETHYL AND PENOXSULAM HERBICIDES.

  2020cites this paper
• Morphology, Ultrastructure, and Molecular Phylogeny of Aphelidium collabens sp. nov. (Aphelida), a Parasitoid of a Green Alga Coccomyxa sp.

  2020cites this paper
• CITED4 Protects Against Adverse Remodeling in Response to Physiological and Pathological Stress

  2020cites this paper
• Identification of select G-protein coupled receptors as regulators of the ER-mitochondria contacts by drug screening

  2020cites this paper
• Purification of age-distinct insulin secretory granules through antigen restriction

  2020cites this paper
• Fluorine and white clover: Assessing fluorine's impact on Rhizobium leguminosarum.

  2020cites this paper
• Cooperative treatment effectiveness of ATR and HSP90 inhibition in Ewing’s sarcoma cells

  2020cites this paper
• Acquired demyelination but not genetic developmental defects in myelination leads to brain tissue stiffness changes

  2020cites this paper
• Electron Microscopy for the Characterization of Soft Tissue Mineralization

  2020cites this paper
• Mitochondrial dynamics in yeast with repressed adenine nucleotide translocator AAC2.

  2020cites this paper
• Fatty acids profiles and estimation of the biodiesel quality parameters from Rhodotorula spp. from Antarctica

  2020cites this paper
• Development of Embryo Suspensors for Five Genera of Crassulaceae with Special Emphasis on Plasmodesmata Distribution and Ultrastructure

  2020cites this paper
• Rap1-GTPases control mTORC1 activity by coordinating lysosome organization with amino acid availability

  2020cites this paper
• Assessment of Mitochondrial Dysfunction in a Murine Model of Supraspinatus Tendinopathy.

  2020cites this paper
• Egg structure of five antarctoperlarian stoneflies (Insecta: Plecoptera, Antarctoperlaria).

  2020cites this paper
• Analysis of the Most Common Software and Hardware Vulnerabilities in Microprocessor Systems

  2020cites this paper
• Structure and function of the stylets of hematophagous Triatominae (Hemiptera: Reduviidae), with special reference to Dipetalogaster maxima.

  2020cites this paper
• Analysis of Integrated Circuits of Electronic Systems by Using Physical Control Methods

  2020cites this paper
• G protein-coupled receptor signaling regulates ER-mitochondria contacts

  2020cites this paper
• All-optical correlative micro-spectroscopies in the investigation of stromal collagen morpho-mechanics

  2020cites this paper
• Bryoclavula phycophila gen. et sp. nov. belonging to a novel lichenized lineage in Cantharellales (Basidiomycota)

  2020cites this paper
• Correlative light and electron microscopy of wall formation in Eimeria nieschulzi

  2020cites this paper
• Retinal Degeneration: Methods and Protocols

  2019cites this paper
• Taxonomic revision of the genus Zygorhizidium: Zygorhizidiales and Zygophlyctidales ord. nov. (Chytridiomycetes, Chytridiomycota)

  2019cites this paper
• The Ultrastructure of Chrysaora quinquecirrha Strobilae

  2019cites this paper
• Molecular and structural insights into skin collagen reveals several factors that influence its architecture.

  2019cites this paper
• Chemical Fixation

  2019cites this paper
• Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell.

  2019cites this paper
• Remodeling of ER–plasma membrane contact sites but not STIM1 phosphorylation inhibits Ca2+ influx in mitosis

  2019cites this paper
• Characterization of a GDP-Fucose Transporter and a Fucosyltransferase Involved in the Fucosylation of Glycoproteins in the Diatom Phaeodactylum tricornutum

  2019cites this paper
• Opposing functions for retromer and Rab11 in extracellular vesicle cargo traffic at presynaptic terminals

  2019cites this paper