Fighting Virus and Parasites with Fish Cytotoxic Cells

Cell-mediated cytotoxic (CMC) activity is the main cellular immunological response to kill tumor cells, virus-infected cells and parasites (Groscurth, 1989). In mammalian species this is carried out by several leucocyte populations depending on the non-specific/innate and specific/adaptive immune response. Among the last ones, the CMC activity is carried out by cytotoxic T lymphocytes (CTLs), expressing the co-receptor CD8, after repeated antigen contact and restricted to major histocompatibility complex (MHC) I. Among the innate cytotoxic cells, acting without previous neither sensitization nor MHC I restriction, the most important are the natural killer (NK) cells, which consist on large granular lymphocytes (markers: CD16/56+CD8-). However, other cell types such as the lymphokine-activated killer cells (LAK), adherent lymphokine-activated killer cells (ALAK), antibody-dependent cytotoxic cells (ADCC), macrophages, neutrophils and acidophils are also responsible for innate CMC activity (Groscurth, 1989). This CMC activity has been described in all the vertebrate animals with substantial differences. In the case of fish, the first vertebrate group showing both innate and adaptive immune system, they are not an exception. However, deeper studies are needed to clearly understand the appearance and evolution of the fish cytotoxic cells and their activity from an evolutionary point of view. Furthermore, the great potential of aquaculture industry and lack of commercial antiviral and antiparasitic vaccines for fish make necessary to increase the knowledge on the CMC activity of fish.

• Publication year

  2012

• Venue

  Unknown venue

• Publication date

  2012-04-11

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.5772/28122
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Viral hemorrhagic septicaemia virus (VHSV) up-regulates the cytotoxic activity and the perforin/granzyme pathway in the rainbow trout RTS11 cell line.

  2011cited by this paper
• Perforin-dependent cytotoxic mechanism in killing by CD8 positive T cells in ginbuna crucian carp, Carassius auratus langsdorfii.

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• Cytotoxic CD8α+ leucocytes have heterogeneous features in antigen recognition and class I MHC restriction in grouper.

  2011cited by this paper
• Cytotoxic T cells in teleost fish.

  2011cited by this paper
• Evaluating antigen-specific cytotoxicity of CD8+ T cells in fish by granzyme B-like activity.

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• The expression of CD8α discriminates distinct T cell subsets in teleost fish.

  2011influential reference
• Immune response of gilthead seabream (Sparus aurata) following experimental infection with Aeromonas hydrophila.

  2011cited by this paper
• Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus.

  2010cited by this paper
• Expression profiles of genes associated with immune response and oxidative stress in Atlantic cod, Gadus morhua head kidney leukocytes modulated by live and heat-inactivated intestinal bacteria.

  2010cited by this paper
• Effect of probiotics enriched diet on Paralichthys olivaceus infected with lymphocystis disease virus (LCDV).

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• Identification and characterization of common carp (Cyprinus carpio L.) granzyme A/K, a cytotoxic cell granule-associated serine protease.

  2010cited by this paper
• An active DNA vaccine against infectious pancreatic necrosis virus (IPNV) with a different mode of action than fish rhabdovirus DNA vaccines.

  2010cited by this paper
• Induction of virus-specific cell-mediated cytotoxic responses of isogeneic ginbuna crucian carp, after oral immunization with inactivated virus.

  2010cited by this paper
• Transcription of immune genes upon challenge with viral hemorrhagic septicemia virus (VHSV) in DNA vaccinated rainbow trout (Oncorhynchus mykiss).

  2009cited by this paper
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  2009cited by this paper
• Effects of dietary vitamin D3 administration on innate immune parameters of seabream (Sparus aurata L.).

  2009cited by this paper
• Alloantigen-specific killing is mediated by CD8-positive T cells in fish.

  2009cited by this paper
• Cloning and characterization of the trout perforin.

  2009cited by this paper
• In vitro generation of viral-antigen dependent cytotoxic T-cells from ginbuna crucian carp, Carassius auratus langsdorfii.

  2009cited by this paper
• Monospecies and multispecies probiotic formulations produce different systemic and local immunostimulatory effects in the gilthead seabream (Sparus aurata L.).

  2008cited by this paper
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  2008cited by this paper
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  2008cited by this paper
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• Effects of the organochlorines p,p'-DDE and lindane on gilthead seabream leucocyte immune parameters and gene expression.

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  2008cited by this paper
• In vivo actions of melatonin on the innate immune parameters in the teleost fish gilthead seabream

  2008cited by this paper
• Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus.

  2008influential reference
• NK-like and oxidative burst activities are the main early cellular innate immune responses activated after virus inoculation in reservoir fish.

  2008cited by this paper
• Effects of inulin on gilthead seabream (Sparus aurata L.) innate immune parameters.

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• Oral delivery of live yeast Debaryomyces hansenii modulates the main innate immune parameters and the expression of immune-relevant genes in the gilthead seabream (Sparus aurata L.).

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• Transcription of signal-3 cytokines, IL-12 and IFN alpha beta, coincides with the timing of CD8 alpha beta up-regulation during viral infection of common carp (Cyprinus carpio L).

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• Unmethylated CpG motifs mimicking bacterial DNA triggers the local and systemic innate immune parameters and expression of immune-relevant genes in gilthead seabream.

  2008cited by this paper
• Early local and systemic innate immune responses in the teleost gilthead seabream after intraperitoneal injection of whole yeast cells.

  2007cited by this paper
• Cell-mediated cytotoxicity in rainbow trout, Oncorhynchus mykiss, infected with viral haemorrhagic septicaemia virus.

  2007influential reference
• Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8beta and CD4-like genes.

  2007cited by this paper
• Genomic organization and expression of CD8α and CD8β genes in fugu Takifugu rubripes

  2007cited by this paper
• Channel catfish, Ictalurus punctatus, CD4-like molecules.

  2007cited by this paper
• Genomic organization and expression of CD8alpha and CD8beta genes in fugu Takifugu rubripes.

  2007cited by this paper
• Modulation of juvenile brook trout (Salvelinus fontinalis) cellular immune system after Aeromonas salmonicida challenge.

  2006cited by this paper
• Quantitative expression (Walbaum) of immunological factors in rainbow trout, Oncorhynchus mykiss (Walbaum), after infection with either Flavobacterium psychrophilum, Aeromonas salmonicida, or infectious haematopoietic necrosis virus.

  2006cited by this paper
• The interferon system of teleost fish.

  2006cited by this paper
• Effect of heat-inactivated fish and non-fish derived probiotics on the innate immune parameters of a teleost fish (Sparus aurata L.).

  2006cited by this paper
• Granzyme-like sequences in bony fish shed light on the emergence of hematopoietic serine proteases during vertebrate evolution.

  2006cited by this paper
• Gilthead seabream (Sparus aurata L.) innate immune response after dietary administration of heat-inactivated potential probiotics.

  2006cited by this paper
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• Cytotoxic activities of fish leucocytes.

  2006influential reference
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  2006cited by this paper
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• Expression profiles of TCRβ and CD8α mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp

  2006cited by this paper
• Cloning and expression analysis of the co-receptor CD8α in sea bream (Sparus aurata L.)

  2006cited by this paper
• Characterisation of salmon and trout CD8alpha and CD8beta.

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• Epithelio-reticular cell thymoma in carp, Cyprinus carpio L: an ultrastructural study.

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• Adaptive cell-mediated cytotoxicity against allogeneic targets by CD8-positive lymphocytes of rainbow trout (Oncorhynchus mykiss).

  2003cited by this paper
• Role of specific cell-mediated cytotoxicity in protecting fish from viral infections.

  2002influential reference
• Channel catfish cytotoxic cells: a mini-review.

  2002influential reference
• Oral administration of yeast, Saccharomyces cerevisiae, enhances the cellular innate immune response of gilthead seabream (Sparus aurata L.).

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• Natural cytotoxic activity in seabream (Sparus aurata L.) and its modulation by vitamin C.

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• Mechanisms of nonspecific cytotoxic cell regulation of apoptosis: cytokine-like activity of Fas ligand.

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• Cytotoxic T cell function in fish.

  2002influential reference
• Novel immune‐type receptor genes

  2001cited by this paper
• Immunomodulatory effects of dietary intake of chitin on gilthead seabream (Sparus aurata L.) innate immune system.

  2001cited by this paper
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  2001influential reference
• Nonspecific cytotoxic cells and innate immunity: regulation by programmed cell death.

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  2001influential reference
• Streptococcus iniae inhibition of apoptosis of nonspecific cytotoxic cells: a mechanism of activation of innate immunity in teleosts.

  2001cited by this paper

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