Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. hepatica antibodies coincided with an intense innate and adaptive cellular immune response, with infiltrating leukocytes and a marked eosinophilia (49%). However, while cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated, these were not statistically different at 18 days post-infection compared to uninfected animals indicating that the immune response is muted and not yet skewed to a Th2 type response that is associated with chronic disease. Proteomic analysis of the peritoneal fluid identified infection-related proteins, including several structural proteins derived from the liver extracellular matrix, connective tissue and epithelium, and proteins related to the immune system. Periostin and vascular cell adhesion protein 1 (VCAM-1), molecules that mediate leukocyte infiltration and are associated with inflammatory disorders involving marked eosinophilia (e.g. asthma), were particularly elevated in the peritoneum. Immuno-histochemical studies indicated that the source of periostin and VCAM-1 was the inflamed sheep liver tissue. This study has revealed previously unknown aspects of the immunology and pathogenesis associated with acute fascioliasis in the peritoneum and liver.

• Publication year

  2017

• Venue

  Scientific Reports

• Publication date

  2017-06-05

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/s41598-017-03094-0PMID 28584245PMCID 5459796
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Fasciola hepatica demonstrates high levels of genetic diversity, a lack of population structure and high gene flow: possible implications for drug resistance

  2017cited by this paper
• Principles and Methods of Immunohistochemistry.

  2017cited by this paper
• In Vivo and In Vitro Studies

  2017cited by this paper
• Time-Course Study of the Transcriptome of Peripheral Blood Mononuclear Cells (PBMCs) from Sheep Infected with Fasciola hepatica

  2016cited by this paper
• Transcriptomic Study on Ovine Immune Responses to Fasciola hepatica Infection

  2016cited by this paper
• Cross Talk Between Inflammation and Extracellular Matrix Following Myocardial Infarction

  2015cited by this paper
• Sputum periostin in patients with different severe asthma phenotypes

  2015cited by this paper
• Gene Expression Profile in the Liver of BALB/c Mice Infected with Fasciola hepatica

  2015cited by this paper
• Fasciola hepatica vaccine: We may not be there yet but we’re on the right road

  2015cited by this paper
• Periostin: its role in asthma and its potential as a diagnostic or therapeutic target

  2015cited by this paper
• Transcriptional analysis identifies key genes involved in metabolism, fibrosis/tissue repair and the immune response against Fasciola hepatica in sheep liver

  2015cited by this paper
• The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution

  2015cited by this paper
• Type VI Collagen: Its Biology and Value as a Biomarker of Hepatic Fibrosis

  2015cited by this paper
• Matricellular protein periostin contributes to hepatic inflammation and fibrosis.

  2015cited by this paper
• Sputum periostin in patients with different severe asthma phenotypes

  2015cited by this paper
• Responses of the spleen to intraamniotic lipopolysaccharide exposure in fetal sheep

  2015cited by this paper
• 2016 update of the PRIDE database and its related tools

  2015cited by this paper
• Liver fluke vaccines in ruminants: strategies, progress and future opportunities.

  2014cited by this paper
• Vascular cell adhesion molecule 1 expression by biliary epithelium promotes persistence of inflammation by inhibiting effector T‐cell apoptosis

  2014cited by this paper
• Eosinophils Are Important for Protection, Immunoregulation and Pathology during Infection with Nematode Microfilariae

  2014cited by this paper
• Periostin, a multifunctional matricellular protein in inflammatory and tumor microenvironments.

  2014cited by this paper
• The sheep genome illuminates biology of the rumen and lipid metabolism

  2014cited by this paper
• Periostin, a novel biomarker of TH2-driven asthma

  2014cited by this paper
• Clinical and biological markers of asthma control

  2014cited by this paper
• Factor VIII-Related Antigen Detects Phenotypic Change of Sinusoidal to Vascular Endothelium in Hepatic Fibrosis of Elderly Cadavers

  2014cited by this paper
• Intraamniotic Lipopolysaccharide Exposure Changes Cell Populations and Structure of the Ovine Fetal Thymus

  2013cited by this paper
• Periostin: A Matricellular Protein Involved in Peritoneal Injury During Peritoneal Dialysis

  2013cited by this paper
• Immunomodulatory molecules of Fasciola hepatica: candidates for both vaccine and immunotherapeutic development.

  2013cited by this paper
• Recent advances in the diagnosis, impact on production and prediction of Fasciola hepatica in cattle

  2013cited by this paper
• Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

  2013cited by this paper
• Periostin Responds to Mechanical Stress and Tension by Activating the MTOR Signaling Pathway

  2013cited by this paper
• α(M)β(2) integrin-mediated adhesion and motility of IL-5-stimulated eosinophils on periostin.

  2013cited by this paper
• Early and late peritoneal and hepatic changes in goats immunized with recombinant cathepsin L1 and infected with Fasciola hepatica.

  2013cited by this paper
• Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica.

  2013cited by this paper
• Impact of uremic environment on peritoneum: a proteomic view.

  2012cited by this paper
• Periostin promotes chronic allergic inflammation in response to Th2 cytokines.

  2012cited by this paper
• Principles and methods of immunohistochemistry.

  2011cited by this paper
• Periostin and osteopontin are overexpressed in chronically inflamed sinuses

  2011cited by this paper
• Liver fluke control in sheep.

  2011cited by this paper
• Periostin Regulates Goblet Cell Metaplasia in a Model of Allergic Airway Inflammation

  2011cited by this paper
• Periostin is a systemic biomarker of eosinophilic airway inflammation in asthmatic patients.

  2011cited by this paper
• In vivo studies of the early, peritoneal, cellular and free radical response in rats infected with Fasciola hepatica by flow cytometric analysis.

  2011cited by this paper
• Hyperendemic human fascioliasis in Andean valleys: an altitudinal transect analysis in children of Cajamarca province, Peru.

  2011cited by this paper
• IL-4 and TGF-β1 Counterbalance One Another while Regulating Mast Cell Homeostasis

  2010cited by this paper
• Heat-induced antigen retrieval for immunohistochemical reactions in routinely processed paraffin sections.

  2010cited by this paper
• Elucidating the transcriptome of Fasciola hepatica - a key to fundamental and biotechnological discoveries for a neglected parasite.

  2010cited by this paper
• Roles of epithelial cell-derived periostin in TGF-β activation, collagen production, and collagen gel elasticity in asthma

  2010cited by this paper
• Expression of the extracellular matrix protein periostin in liver tumours and bile duct carcinomas

  2010cited by this paper
• Improving animal and human health through understanding liver fluke immunology

  2010cited by this paper
• Bovine Genome Database: integrated tools for genome annotation and discovery

  2010cited by this paper
• Control of bovine fasciolosis in dairy cattle in Switzerland with emphasis on pasture management.

  2010cited by this paper
• Bovine Genome Database: supporting community annotation and analysis of the Bos taurus genome

  2010cited by this paper
• Survey of transcripts expressed by the invasive juvenile stage of the liver fluke Fasciola hepatica

  2010cited by this paper
• Coordinating innate and adaptive immunity in Fasciola hepatica infection: implications for control.

  2010cited by this paper
• Faculty Opinions recommendation of Roles of epithelial cell-derived periostin in TGF-beta activation, collagen production, and collagen gel elasticity in asthma.

  2010cited by this paper
• Integrative Proteomic Analysis of Serum and Peritoneal Fluids Helps Identify Proteins that Are Up-Regulated in Serum of Women with Ovarian Cancer

  2010cited by this paper
• Infection with a Helminth Parasite Attenuates Autoimmunity through TGF-β-Mediated Suppression of Th17 and Th1 Responses1

  2009cited by this paper
• Co-Infection of cattle with Fasciola hepatica and Mycobacterium bovis- immunological consequences.

  2009cited by this paper
• An Integrated Transcriptomics and Proteomics Analysis of the Secretome of the Helminth Pathogen Fasciola hepatica

  2009cited by this paper
• Proteomic analysis of peritoneal fluid in fertile and infertile women with endometriosis.

  2009cited by this paper
• Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control.

  2009cited by this paper
• IL‐10 and TGF‐β1 are associated with variations in fluke burdens following experimental fasciolosis in sheep

  2009cited by this paper
• Evaluation of the flukicide treatment policy for dairy cattle in Galicia (NW Spain).

  2008cited by this paper
• Eosinophils: Biological Properties and Role in Health and Disease

  2008cited by this paper
• Helminth 2‐Cys peroxiredoxin drives Th2 responses through a mechanism involving alternatively activated macrophages

  2008cited by this paper
• The role of the complement anaphylatoxins in the recruitment of eosinophils.

  2007cited by this paper
• Discovery and validation of serum biomarkers expressed over the first twelve weeks of Fasciola hepatica infection in sheep

  2007cited by this paper
• Comparative Proteomics of Excretory-Secretory Proteins Released by the Liver Fluke Fasciola hepatica in Sheep Host Bile and during in Vitro Culture ex Host*S

  2007cited by this paper
• Analysis of Eosinophil Turnover In Vivo Reveals Their Active Recruitment to and Prolonged Survival in the Peritoneal Cavity1

  2007cited by this paper
• Periostin induces proliferation of differentiated cardiomyocytes and promotes cardiac repair

  2007cited by this paper
• Peritoneal Lavage Cells of Indonesian Thin-Tail Sheep Mediate Antibody-Dependent Superoxide Radical Cytotoxicity In Vitro against Newly Excysted Juvenile Fasciola gigantica but Not Juvenile Fasciola hepatica

  2007cited by this paper
• Understanding triclabendazole resistance.

  2007cited by this paper
• Distribution and Origin of the Basement Membrane Component Perlecan in Rat Liver and Primary Hepatocyte Culture

  2007cited by this paper
• Proteomic analysis of peritoneal fluid in women with endometriosis.

  2007cited by this paper
• Haemonchus contortus (Nematoda: Trichostrongylidae) infection in lambs elicits an unequivocal Th2 immune response.

  2006cited by this paper
• Peritoneal fluid analysis in adult, nonpregnant Awassi sheep.

  2006cited by this paper
• Experimental Fasciola hepatica Infection Alters Responses to Tests Used for Diagnosis of Bovine Tuberculosis

  2006cited by this paper
• Eosinophil Activity in Schistosoma mansoni Infections In Vivo and In Vitro in Relation to Plasma Cytokine Profile Pre- and Posttreatment with Praziquantel

  2006cited by this paper
• Bile acids induce adhesion molecule expression in endothelial cells through activation of reactive oxygen species, NF-kappaB, and p38.

  2006cited by this paper
• Natural regulatory T cells and parasites: a common quest for host homeostasis

  2006cited by this paper
• Periostin: a novel component of subepithelial fibrosis of bronchial asthma downstream of IL-4 and IL-13 signals.

  2006cited by this paper
• Thioredoxin Peroxidase Secreted by Fasciola hepatica Induces the Alternative Activation of Macrophages

  2005cited by this paper
• In vivo imaging of tissue eosinophilia and eosinopoietic responses to schistosome worms and eggs.

  2005cited by this paper
• Epidemiology of fascioliasis in human endemic areas

  2005cited by this paper
• Fascioliasis and other plant-borne trematode zoonoses.

  2005cited by this paper
• Early and transient cytotoxic response of peritoneal cells from Fasciola hepatica-infected rats.

  2004cited by this paper
• IFN-gamma/STAT1 acts as a proinflammatory signal in T cell-mediated hepatitis via induction of multiple chemokines and adhesion molecules: a critical role of IRF-1.

  2004cited by this paper
• Cathepsin L1, the Major Protease Involved in Liver Fluke (Fasciola hepatica) Virulence

  2004cited by this paper
• A statistical model for identifying proteins by tandem mass spectrometry.

  2003cited by this paper
• Toll‐Like receptor 4 mediates inflammatory signaling by bacterial lipopolysaccharide in human hepatic stellate cells

  2003cited by this paper
• Pathological and immunohistochemical study of the liver and hepatic lymph nodes of sheep chronically reinfected with Fasciola hepatica, with or without triclabendazole treatment.

  2002cited by this paper
• Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search.

  2002cited by this paper
• Managing parasiticide resistance.

  2001cited by this paper
• A new mathematical model for relative quantification in real-time RT-PCR.

  2001cited by this paper
• Matrix as a modulator of hepatic fibrogenesis.

  2001cited by this paper
• Interleukin-18 binding protein: a novel modulator of the Th1 cytokine response.

  1999cited by this paper
• Fasciola hepatica Suppresses a Protective Th1 Response against Bordetella pertussis

  1999cited by this paper
• Development of vaccines against "Fasciola hepatica"

  1999cited by this paper
• Immune responses of cattle to experimental anti-Fasciola hepatica vaccines.

  1999cited by this paper
• Soluble Collagen VI Drives Serum-starved Fibroblasts through S Phase and Prevents Apoptosis via Down-regulation of Bax*

  1999cited by this paper

• Prevalence and risk factors of ovine and caprine fasciolosis in Ethiopia: a systematic review and meta-analysis

  2025cites this paper
• Appraisal of the Use of Proteomics Methodological Approaches and Technologies on Sheep and Goat Research and Clinical Work

  2025cites this paper
• Prevalence and risk factors of sheep and goats fasciolosis in Ethiopia: A systematic review and meta-analysis

  2025cites this paper
• Prevalence and associated factors of schistosomiasis among domestic ruminants in Ethiopia: a systematic review and meta-analysis

  2025cites this paper
• DIAGNOSTIC AND PROGNOSTIC ROLE OF PERIOSTIN IN RESPIRATORY SYSTEM DISEASE COMPLEX IN CALVES

  2024cites this paper
• Fasciola hepatica Soluble Antigen (FhAg)-Induced NETs Under Hypoxic Conditions Exert Cytotoxic Effects on Hepatic Cells In Vitro

  2024cites this paper
• Both host and parasite non-coding RNAs co-ordinate the regulation of macrophage gene expression to reduce pro-inflammatory immune responses and promote tissue repair pathways during infection with fasciola hepatica

  2024cites this paper
• An Update on the Pathogenesis of Fascioliasis: What Do We Know?

  2024cites this paper
• Fasciolosis: pathogenesis, host-parasite interactions, and implication in vaccine development

  2023cites this paper
• Fasciola hepatica soluble antigens (FhAg) induce ovine PMN innate immune reactions and NET formation in vitro and in vivo

  2023cites this paper
• Fasciolosis—An Increasing Challenge in the Sheep Industry

  2022cites this paper
• Exploring the role of macrophages in determining the pathogenesis of liver fluke infection

  2022cites this paper
• Host-parasite interactions mediated by cross-species microRNAs.

  2022cites this paper
• Two Distinct Superoxidase Dismutases (SOD) Secreted by the Helminth Parasite Fasciola hepatica Play Roles in Defence against Metabolic and Host Immune Cell-Derived Reactive Oxygen Species (ROS) during Growth and Development

  2022cites this paper
• The Host Peritoneal Cavity Harbors Prominent Memory Th2 and Early Recall Responses to an Intestinal Nematode

  2022cites this paper
• Timing of Transcriptomic Peripheral Blood Mononuclear Cell Responses of Sheep to Fasciola hepatica Infection Differs From Those of Cattle, Reflecting Different Disease Phenotypes

  2021cites this paper
• An Evaluation of the Fasciola hepatica miRnome Predicts a Targeted Regulation of Mammalian Innate Immune Responses

  2021cites this paper
• Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses

  2021cites this paper
• Autonomous Non Antioxidant Roles for Fasciola hepatica Secreted Thioredoxin-1 and Peroxiredoxin-1

  2021cites this paper
• Immunodiagnosis of cattle fascioliasis using a 27 kDa Fasciola gigantica antigen

  2021cites this paper
• Immune response in Santa Inês sheep during Fasciola hepatica infection

  2021cites this paper
• Pathogenicity and virulence of the liver flukes Fasciola hepatica and Fasciola Gigantica that cause the zoonosis Fasciolosis

  2021cites this paper
• The application of proteomics in the diagnosis and treatment of bronchial asthma.

  2020cites this paper
• Pathomorphological and Morphometric Studies of Bovine Liver Infected with Fascioles

  2020cites this paper
• Regulation of the Fasciola hepatica newly excysted juvenile cathepsin L3 (FhCL3) by its propeptide: a proposed ‘clamp-like’ mechanism of binding and inhibition

  2020cites this paper
• Insights into Fasciola hepatica Juveniles: Crossing the Fasciolosis Rubicon.

  2020cites this paper
• Calicophoron daubneyi—The Path Toward Understanding Its Pathogenicity and Host Interactions

  2020cites this paper
• Fasciola hepatica serine protease inhibitor family (serpins): Purposely crafted for regulating host proteases

  2020cites this paper
• Development of SYBR Green real-time PCR for diagnosis of fasciolosis in sheep.

  2020cites this paper
• Eosinophils and Macrophages within the Th2-Induced Granuloma: Balancing Killing and Healing in a Tight Space

  2019cites this paper
• Allele substitution and dominance effects of CD166/ALCAM gene polymorphisms for endoparasite resistance and test-day traits in a small cattle population using logistic regression analyses

  2019cites this paper
• The cathepsin-like cysteine peptidases of trematodes of the genus Fasciola.

  2019cites this paper
• Study of peritoneal macrophage immunophenotype in sheep experimentally infected with Fasciola hepatica.

  2018cites this paper
• Estudio del hígado y líquido peritoneal en fases tempranas de ovejas infectadas experimentalmente con fasciola hepatica

  2018cites this paper
• Fh15 Blocks the Lipopolysaccharide-Induced Cytokine Storm While Modulating Peritoneal Macrophage Migration and CD38 Expression within Spleen Macrophages in a Mouse Model of Septic Shock

  2018cites this paper
• Advances in Fasciola hepatica research using 'omics' technologies.

  2018influential citation
• Expression of free radicals by peritoneal cells of sheep during the early stages of Fasciola hepatica infection

  2018cites this paper
• Reasons to Be Nervous about Flukicide Discovery.

  2017cites this paper