The Emerging Importance of IgG Fab Glycosylation in Immunity

No abstract is available for this paper.

• Publication year

  2016

• Venue

  Journal of Immunology

• Publication date

  2016-02-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.1502136PMID 26851295
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• DC-SIGN-expressing macrophages trigger activation of mannosylated IgM B-cell receptor in follicular lymphoma.

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• Analysis and Functional Consequences of Increased Fab-Sialylation of Intravenous Immunoglobulin (IVIG) after Lectin Fractionation

  2012cited by this paper
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  2009cited by this paper
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  2009cited by this paper
• Increasing the sialylation of therapeutic glycoproteins: the potential of the sialic acid biosynthetic pathway.

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• Remarkable selective glycosylation of the immunoglobulin variable region in follicular lymphoma.

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  2007cited by this paper
• Protective mechanisms of IVIG.

  2007cited by this paper
• Structural characterization of N-linked oligosaccharides on monoclonal antibody cetuximab by the combination of orthogonal matrix-assisted laser desorption/ionization hybrid quadrupole-quadrupole time-of-flight tandem mass spectrometry and sequential enzymatic digestion.

  2007cited by this paper
• Antibody therapeutics:

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• The impact of glycosylation on the biological function and structure of human immunoglobulins.

  2007cited by this paper
• Contrasting glycosylation profiles between Fab and Fc of a human IgG protein studied by electrospray ionization mass spectrometry.

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• Differential glycosylation of polyclonal IgG, IgG-Fc and IgG-Fab isolated from the sera of patients with ANCA-associated systemic vasculitis.

  2006cited by this paper
• The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins.

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• Anti-Inflammatory Activity of Immunoglobulin G Resulting from Fc Sialylation

  2006cited by this paper
• Variable region heavy chain glycosylation determines the anticoagulant activity of a factor VIII antibody

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• Interleukin-6 and dexamethasone modulate in vitro asymmetric antibody synthesis and UDP-Glc glycoprotein glycosyltransferase activity.

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• Asymmetric antibodies (AAb) in the female reproductive tract.

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  2000cited by this paper
• Position effects of variable region carbohydrate on the affinity and in vivo behavior of an anti-(1-->6) dextran antibody.

  1999influential reference
• Lectin analysis of human immunoglobulin G N-glycan sialylation

  1999cited by this paper
• Variable domain-linked oligosaccharides of a human monoclonal IgG: structure and influence on antigen binding.

  1999cited by this paper
• The variable domain glycosylation in a monoclonal antibody specific to GnRH modulates antigen binding.

  1997cited by this paper
• Site-specific glycosylation of human immunoglobulin G is altered in four rheumatoid arthritis patients.

  1996cited by this paper
• Glycosylation of the variable region of immunoglobulin G--site specific maturation of the sugar chains.

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• IgG asymmetric molecules with antipaternal activity isolated from sera and placenta of pregnant human.

  1991influential reference
• Antibody variable region glycosylation: position effects on antigen binding and carbohydrate structure.

  1991cited by this paper
• Asymmetrically glycosylated IgG isolated from non-immune human sera.

  1989cited by this paper
• Nonprecipitating asymmetric antibodies.

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  1986cited by this paper
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  1985cited by this paper

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