Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Cytometry Part A

• Publication date

  2005-09-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1002/cyto.a.20167PMID 16080188
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Rapid detection of phosphotyrosine proteins by flow cytometric analysis in Bcr‐Abl–positive cells

  2004cited by this paper
• Bias Estimation in Method Comparison Studies

  2004cited by this paper
• Analysis of protein phosphorylation and cellular signaling events by flow cytometry: techniques and clinical applications.

  2004cited by this paper
• Single cell profiling of potentiated phospho-protein networks in cancer cells.

  2004cited by this paper
• Intracellular phospho‐protein staining techniques for flow cytometry: Monitoring single cell signaling events

  2003cited by this paper
• Immunoreactivity of Stat5 phosphorylated on tyrosine as a cell‐based measure of Bcr/Abl kinase activity

  2003cited by this paper
• Flow cytometric measurement of phosphorylated STAT5 in AML: lack of specific association with FLT3 internal tandem duplications.

  2003cited by this paper
• Statistical analysis and optimal classification of blood cell populations using Gaussian distributions

  2003cited by this paper
• Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry

  2002cited by this paper
• Setting performance goals and evaluating total analytical error for diagnostic assays.

  2002cited by this paper
• Magnetic relaxation switches capable of sensing molecular interactions

  2002cited by this paper
• Measurement of MAP kinase activation by flow cytometry using phospho-specific antibodies to MEK and ERK: potential for pharmacodynamic monitoring of signal transduction inhibitors.

  2001cited by this paper
• A unified procedure for conservative (morphology) and integral (DNA and immunophenotype) cell staining for flow cytometry.

  2001cited by this paper
• Comparative study of five commercial reagents for preparing normal and leukaemic lymphocytes for immunophenotypic analysis by flow cytometry.

  1999cited by this paper
• Rapid single-step method for flow cytometric detection of surface and intracellular antigens using whole blood.

  1996influential reference
• Analysis of intracellular proteins.

  1994cited by this paper
• Flow cytometric titration of retroviral expression vectors: comparison of methods for analysis of immunofluorescence histograms derived from cells expressing low antigen levels.

  1993cited by this paper
• Fixation of mammalian cells for flow cytometric evaluation of DNA content and nuclear immunofluorescence.

  1992cited by this paper
• Intracellular antigen staining: Quantitative immunofluorescence

  1991cited by this paper
• Statistical methods for assessing agreement between two methods of clinical measurement.

  1986influential reference
• Use and interpretation of common statistical tests in method-comparison studies.

  1973cited by this paper
• AKUFO AND IBARAPA.

  1965cited by this paper

• Multiplexed Immunophenotyping for Innate Activation Assessment Detects Single-Cell Responses to Immunomodulatory Nucleic Acid Impurities in Therapeutics

  2026cites this paper
• The effect on canine erythrocyte osmotic fragility under different temperature variations and storage times

  2025cites this paper
• Analytical Comparison of Three Commercial Lysing Reagents for Full‐Spectrum Flow Cytometric Immunophenotyping

  2025cites this paper
• Optimized protocol for intracellular labeling of red blood cells with anti-hemoglobin F for confocal microscopy analysis.

  2025cites this paper
• Flow cytometry of DNMT1 as a biomarker of hypomethylating therapies

  2024cites this paper
• Guidelines on Antibody Use for Physiology Research.

  2024cites this paper
• Photothermal Enhancement of Ion Current for Red Blood Cell Flow Cytometry.

  2024cites this paper
• Overcoming fixation and permeabilization challenges in flow cytometry by optical barcoding and multi-pass acquisition

  2024cites this paper
• Analysis of the multiparametric cell cycle data.

  2024cites this paper
• Optimization of Formaldehyde Fixative Concentration for Individual Blood Cells to Develop a Stabilized Blood Control for Automated Hematology Analyzers

  2023cites this paper
• How fixation affects the results of lymph node immunophenotyping by flow cytometry

  2023cites this paper
• Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

  2022cites this paper
• Crizotinib has Preclinical Efficacy in Philadelphia-negative Myeloproliferative Neoplasms.

  2022cites this paper
• Assessment of Host Immune Responses to Fungal Pathogens

  2022cites this paper
• A Comprehensive Atlas of Immunological Differences Between Humans, Mice, and Non-Human Primates

  2022cites this paper
• Comparative flow cytometry-based immunophenotyping analysis of peripheral blood leukocytes before and after fixation with paraformaldehyde.

  2022cites this paper
• The Radio-mitigative Effect of CpG-Oligodeoxynucleotides on Mice After Exposure to Carbon Ions Radiation.

  2021cites this paper
• Rac1/pSTAT3 expression: A pharmacodynamic marker panel as a first step toward optimization of thiopurine therapy in inflammatory bowel disease patients

  2021cites this paper
• A field method for cryopreservation of whole blood from a finger prick for later analysis with flow cytometry.

  2021cites this paper
• Comprehensive phenotyping of endothelial cells using flow cytometry 2: Human

  2020cites this paper
• Defective Bcl‐2 expression in memory B cells from common variable immunodeficiency patients

  2020cites this paper
• Application of microfluidics in cancer treatment

  2020cites this paper
• Circulating-tumor DNA from Two Types of Blood Collection Tubes for Monitoring Tumor Burden Validated with Esophageal Squamous Cell Carcinoma Patients

  2020cites this paper
• Evaluation of B‐cell intracellular signaling by monitoring the PI3K‐Akt axis in patients with common variable immunodeficiency and activated phosphoinositide 3‐kinase delta syndrome

  2020cites this paper
• Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

  2019cites this paper
• Impact of blood storage and sample handling on quality of high dimensional flow cytometric data in multicenter clinical research.

  2019cites this paper
• Variation of Immune Cell Responses in Humans Reveals Sex-Specific Coordinated Signaling Across Cell Types

  2019cites this paper
• Relevance of Antibody Validation for Flow Cytometry

  2019cites this paper
• Mass Cytometric Cell Cycle Analysis.

  2019cites this paper
• Gene Expression Analysis of the Perinatal Heart and the Identification of MiR-205 as a Regulator of Cardiomyocyte Maturation

  2019cites this paper
• Immunophenotyping of Circulating Endothelial Cells and Endothelial Microparticles.

  2019cites this paper
• A comprehensive atlas of immunological differences between humans, mice and non-human primates

  2019cites this paper
• Cell type-specific monoclonal antibody cross-reactivity screening in non-human primates and development of comparative immunophenotyping panels for CyTOF

  2019cites this paper
• Immunophenotyping by Mass Cytometry.

  2019cites this paper
• A multicolour flow cytometric assay for c-MYC protein in B-cell lymphoma

  2018cites this paper
• Development of immuno-flowFISH by imaging flow cytometry for the analysis of haematoligical maligancies

  2018cites this paper
• Cell Cycle Analysis by Mass Cytometry.

  2018cites this paper
• A rapid and efficient method for the isolation of postnatal murine cardiac myocyte and fibroblast cells.

  2018cites this paper
• Cellular endogenous NAD(P)H fluorescence as a label‐free method for the identification of erythrocytes and reticulocytes

  2018cites this paper
• Multiparameter Cell Cycle Analysis.

  2018cites this paper
• A phase 2 study of ofatumumab (Arzerra®) in combination with a pan-AKT inhibitor (afuresertib) in previously treated patients with chronic lymphocytic leukemia (CLL)*

  2018cites this paper
• A low dose lipid infusion is sufficient to induce insulin resistance and a pro-inflammatory response in human subjects

  2018cites this paper
• Rapid comparative immunophenotyping of human mesenchymal stromal cells by a modified fluorescent cell barcoding flow cytometric assay

  2018cites this paper
• Relative quantification of beta‐adrenergic receptor in peripheral blood cells using flow cytometry

  2018cites this paper
• Systematic characterization of basophil anergy

  2017cites this paper
• Guidelines for the use of flow cytometry and cell sorting in immunological studies.

  2017cites this paper
• Señalización intracelular y perfil epigenético de linfocitos B en la inmunodeficiencia común variable

  2017cites this paper
• ERK and ROCK functionally interact in a signaling network that is compensationally upregulated in Spinal Muscular Atrophy.

  2017cites this paper
• Serum Derived Transfer Factor Stimulates the Innate Immune System to Improve Survival Traits in High Risk Pathogen Scenarios

  2017influential citation
• Propagation of trimethylated H3K27 regulated by polycomb protein EED is required for embryogenesis, hematopoietic maintenance, and tumor suppression

  2016cites this paper
• Performance of a phosphoflow assay to determine phosphorylation of S6 ribosomal protein as a pharmacodynamic read out for mTOR inhibition.

  2016cites this paper
• Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats

  2016cites this paper
• Flow Cytometric Quantification of Peripheral Blood Cell β-Adrenergic Receptor Density and Urinary Endothelial Cell-Derived Microparticles in Pulmonary Arterial Hypertension

  2016cites this paper
• Computational imaging reveals mitochondrial morphology as a biomarker of cancer phenotype and drug response

  2016influential citation
• Immune Cell Profiling of IFN-λ Response Shows pDCs Express Highest Level of IFN-λR1 and Are Directly Responsive via the JAK-STAT Pathway

  2016cites this paper
• Differential antigen expression and aberrant signaling via PI3/AKT, MAP/ERK, JAK/STAT, and Wnt/β catenin pathways in Lin−/CD38−/CD34+ cells in acute myeloid leukemia

  2016cites this paper
• A phase I trial of the aurora kinase inhibitor, ENMD-2076, in patients with relapsed or refractory acute myeloid leukemia or chronic myelomonocytic leukemia

  2016cites this paper
• Vitamin D enhances reactive oxygen intermediates production in phagocytic cells in term and preterm infants

  2016cites this paper
• Therapeutic mechanisms of fibroblast cells in the skin conditions; trends in clinical applications- A Review

  2016cites this paper
• Type 1 Interferon signalling in human intestinal T cells

  2015cites this paper
• Clinical Study Investigating γ H 2 AX as a Biomarker of Radiosensitivity Using Flow Cytometry Methods

  2015cites this paper
• Whole Blood Measurement of Histone Modifications Linked to the Epigenetic Regulation of Gene Expression

  2015cites this paper
• Simultaneous assessment of NF-κB/p65 phosphorylation and nuclear localization using imaging flow cytometry.

  2015cites this paper
• Probiotic Effect of Lebanese Darfiyeh Cheese: A Randomized Case Control Prospective Study in the Elderly

  2015cites this paper
• Standing surface acoustic wave (SSAW)-based cell washing.

  2015cites this paper
• Targeting the PI3 Kinase-mTOR Signaling Pathway in AML

  2015cites this paper
• Genetic and Pharmacologic Evidence That mTOR Targeting Outweighs mTORC1 Inhibition as an Antimyeloma Strategy

  2014cites this paper
• Cancer Biology and Signal Transduction Genetic and Pharmacologic Evidence That mTOR Targeting Outweighs mTORC 1 Inhibition as an Antimyeloma Strategy

  2014cites this paper
• The study of epigenetic mechanisms based on the analysis of histone modification patterns by flow cytoametry

  2014cites this paper
• Modification of radiation‐induced DNA double strand break repair pathways by chemicals extracted from Podophyllum hexandrum: An in vitro study in human blood leukocytes

  2014cites this paper
• Analysis and inhibition of dysregulated pathways in a mouse model of the motoneuron disease Spinal Muscular Atrophy

  2014cites this paper
• Epigenetics by flow!

  2014cites this paper
• Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining

  2014cites this paper
• Analysis of cytokine induced phosphorylation of STAT3 in peripheral blood mononuclear cells by flow cytometric and western blot assays

  2014cites this paper
• Historical Roots of Flow Cytometry

  2014cites this paper
• Genetic and pharmacological evidence that mTOR targeting outweighs mTORC 1 inhibition as an antimyeloma strategy

  2013cites this paper
• Validation of cell‐based fluorescence assays: Practice guidelines from the ICSH and ICCS – part III – analytical issues

  2013cites this paper
• 15 – Flow Cytometry in Oncologic Diagnosis

  2013cites this paper
• Flow cytometry of neural cells.

  2013cites this paper
• Validation of Cell-based Fluorescence Assays: Practice Guidelines from the ICSH and ICCS - Part II - Preanalytical Issues

  2013cites this paper
• Multiparameter Flow Cytometry: Advances in High Resolution Analysis

  2013cites this paper
• Biological effects of trogocytosis on CD4+ T lymphocytes

  2013cites this paper
• Blood biomarkers in metal scrap workers accidentally exposed to ionizing radiation

  2013cites this paper
• Investigating γH2AX as a Biomarker of Radiosensitivity Using Flow Cytometry Methods

  2013cites this paper
• Improved quantification of HIV-1-infected CD4+ T cells using an optimised method of intracellular HIV-1 gag p24 antigen detection.

  2013cites this paper
• Evaluation of interferon-beta neutralizing antibodies in individual patients with multiple sclerosis based on phospho-specific flow cytometry

  2013cites this paper
• An integrin-targeted, pan-isoform, phosphoinositide-3 kinase inhibitor, SF1126, has activity against multiple myeloma in vivo

  2013cites this paper
• Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage

  2013cites this paper
• Dysregulated ERK signal pathway and immune profiles in Fragile X Syndrome

  2012cites this paper
• Validation of a multiparameter flow cytometry method for the determination of phosphorylated extracellular‐signal‐regulated kinase and DNA in circulating tumor cells

  2012cites this paper
• Assay validation of phosphorylated S6 ribosomal protein for a pharmacodynamic monitoring of mTOR‐inhibitors in peripheral human blood

  2012cites this paper
• Systems Biology Approaches Reveal a Specific Interferon-Inducible Signature in HTLV-1 Associated Myelopathy

  2012cites this paper
• Trogocytosis Results in Sustained Intracellular Signaling in CD4+ T Cells

  2012cites this paper
• CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients

  2012cites this paper
• Selective inhibition of extracellular signal-regulated kinases 1/2 blocks nerve growth factor to brain-derived neurotrophic factor signaling and suppresses the development of and reverses already established pain behavior in rats.

  2012cites this paper
• Human CD4+ lymphocytes for antigen quantification: Characterization using conventional flow cytometry and mass cytometry

  2012cites this paper
• Emerging applications of flow cytometry in solid tumor biology.

  2012cites this paper
• Translational biomarker in oncology early clinical development: decision case study for MEK inhibitors in healthy volunteer studies

  2012cites this paper
• Expression of activating transcription factor 2 in inflammatory macrophages in obese adipose tissue

  2012cites this paper
• Analysis of Signalling Network Consequent to FLT3 in AML Patients

  2011cites this paper