Identification of the membrane receptor of a class XIV myosin in Toxoplasma gondii

Apicomplexan parasites exhibit a unique form of substrate-dependent motility, gliding motility, which is essential during their invasion of host cells and during their spread between host cells. This process is dependent on actin filaments and myosin that are both located between the plasma membrane and two underlying membranes of the inner membrane complex. We have identified a protein complex in the apicomplexan parasite Toxoplasma gondii that contains the class XIV myosin required for gliding motility, TgMyoA, its associated light chain, TgMLC1, and two novel proteins, TgGAP45 and TgGAP50. We have localized this complex to the inner membrane complex of Toxoplasma, where it is anchored in the membrane by TgGAP50, an integral membrane glycoprotein. Assembly of the protein complex is spatially controlled and occurs in two stages. These results provide the first molecular description of an integral membrane protein as a specific receptor for a myosin motor, and further our understanding of the motile apparatus underlying gliding motility in apicomplexan parasites.

• Publication year

  2004

• Venue

  Journal of Cell Biology

• Publication date

  2004-05-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/jcb.200311137PMID 15123738PMCID 2172186
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• PlasmoDB: The Plasmodium Genome Resource

  2005cited by this paper
• Actin filament polymerization regulates gliding motility by apicomplexan parasites.

  2003cited by this paper
• Identification of an organelle-specific myosin V receptor

  2003cited by this paper
• PlasmoDB: the Plasmodium genome resource. A database integrating experimental and computational data

  2003cited by this paper
• Aldolase forms a bridge between cell surface adhesins and the actin cytoskeleton in apicomplexan parasites.

  2003influential reference
• Myosin A tail domain interacting protein (MTIP) localizes to the inner membrane complex of Plasmodium sporozoites

  2003influential reference
• Daughter cell assembly in the protozoan parasite Toxoplasma gondii.

  2002cited by this paper
• Proteolytic Processing of TgIMC1 during Maturation of the Membrane Skeleton of Toxoplasma gondii *

  2002influential reference
• Rab27a is an essential component of melanosome receptor for myosin Va.

  2002cited by this paper
• Toxoplasma gondii myosin A and its light chain: a fast, single‐headed, plus‐end‐directed motor

  2002cited by this paper
• Identification of an organelle receptor for myosin-Va

  2002cited by this paper
• Clostridium septicum Alpha-Toxin Is Active against the Parasitic Protozoan Toxoplasma gondii and Targets Members of the SAG Family of Glycosylphosphatidylinositol-Anchored Surface Proteins

  2002influential reference
• ‘The glideosome’: a dynamic complex powering gliding motion and host cell invasion by Toxoplasma gondii

  2002influential reference
• Role of Toxoplasma gondii Myosin A in Powering Parasite Gliding and Host Cell Invasion

  2002cited by this paper
• Characterization of the subpellicular network, a filamentous membrane skeletal component in the parasite Toxoplasma gondii.

  2001influential reference
• Modulation of myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii.

  2001cited by this paper
• Independence and Cooperativity in Rearrangements of a Potassium Channel Voltage Sensor Revealed by Single Subunit Fluorescence

  2000cited by this paper
• A dibasic motif in the tail of a class XIV apicomplexan myosin is an essential determinant of plasma membrane localization.

  2000influential reference
• Structure, function, and regulation of tartrate-resistant acid phosphatase.

  2000cited by this paper
• Identification of mammalian-like purple acid phosphatases in a wide range of plants.

  2000cited by this paper
• Time-lapse video microscopy of gliding motility in Toxoplasma gondii reveals a novel, biphasic mechanism of cell locomotion.

  1999cited by this paper
• Conservation of a Gliding Motility and Cell Invasion Machinery in Apicomplexan Parasites

  1999cited by this paper
• Gene knock-outs and allelic replacements in Toxoplasma gondii: HXGPRT as a selectable marker for hit-and-run mutagenesis.

  1998cited by this paper
• Actin-dependent motility in Cryptosporidium parvum sporozoites.

  1998cited by this paper
• Actomyosin motor in the merozoite of the malaria parasite, Plasmodium falciparum: implications for red cell invasion.

  1998cited by this paper
• Subpellicular microtubules associate with an intramembranous particle lattice in the protozoan parasite Toxoplasma gondii.

  1997cited by this paper
• Participation of myosin in gliding motility and host cell invasion by Toxoplasma gondii

  1997influential reference
• Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite.

  1996cited by this paper
• Domain structure of a mammalian myosin I beta.

  1994cited by this paper
• Homologous recombination and gene replacement at the dihydrofolate reductase-thymidylate synthase locus in Toxoplasma gondii.

  1994cited by this paper
• Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker.

  1993cited by this paper
• Localization and specificity of the phospholipid and actin binding sites on the tail of Acanthamoeba myosin IC

  1992cited by this paper
• Cytoskeleton of Toxoplasma gondii.

  1987cited by this paper
• An actomyosin motor

  1982influential reference
• Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation

  1979cited by this paper

• A Spinster-like Transporter at the Inner Membrane Complex is critical for Toxoplasma gondii cytokinesis, motility and invasion

  2026cites this paper
• Functions of Posttranslational Modifications in Toxoplasma gondii and Their Potential as Drug Targets

  2025cites this paper
• Structure and molecular composition of Toxoplasma gondii pellicle.

  2025cites this paper
• Plasma membrane recycling drives reservoir formation during Toxoplasma gondii intracellular replication

  2025cites this paper
• From nucleation to capping: The lifecycle of an actin filament in Toxoplasma gondii gliding.

  2025cites this paper
• Regulation of the developmental programs in Toxoplasma by a novel SNF2L-containing chromatin remodeling complex

  2025cites this paper
• Quantitative proteomic analysis of the Toxoplasma gondii cytoskeleton and bioinformatic identification of highly antigenic proteins.

  2025cites this paper
• Toxoplasma gondii endodyogeny: how to make perfect daughters.

  2025cites this paper
• Expansion of in vitro Toxoplasma gondii cysts using enzymatically enhanced ultrastructure expansion microscopy

  2024cites this paper
• An inner membrane complex protein IMC1g in Plasmodium berghei is involved in asexual stage schizogony and parasite transmission

  2024cites this paper
• Cytokinetic abscission in Toxoplasma gondii is governed by protein phosphatase 2A and the daughter cell scaffold complex

  2024cites this paper
• Enzymatically enhanced ultrastructure expansion microscopy unlocks expansion of in vitro Toxoplasma gondii cysts

  2024cites this paper
• A novel Plasmodium berghei protein S14 is essential for sporozoite gliding motility and infectivity.

  2024cites this paper
• Emergent actin flows explain distinct modes of gliding motility

  2024influential citation
• BCC0 collaborates with IMC32 and IMC43 to form the Toxoplasma gondii essential daughter bud assembly complex

  2024cites this paper
• Identification of novel biomarkers for anti-Toxoplasma gondii IgM detection and the potential application in rapid diagnostic fluorescent tests

  2024cites this paper
• AP2XII-1 is a negative regulator of merogony and presexual commitment in Toxoplasma gondii

  2023cites this paper
• Plasmodium falciparum GAP40 Plays an Essential Role in Merozoite Invasion and Gametocytogenesis

  2023cites this paper
• The cortical microtubules of Toxoplasma gondii underlie the helicity of parasite movement

  2023cites this paper
• Becker Digital

  2023cites this paper
• Fostering innovation to solve the biomechanics of microbe–host interactions: Focus on the adhesive forces underlying Apicomplexa parasite biology

  2023cites this paper
• Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii

  2023cites this paper
• A novel glideosome-associated protein S14 coordinates sporozoite gliding motility and infectivity in mosquito and mammalian hosts

  2023cites this paper
• The cortical microtubules of Toxoplasma contribute to the helicity, but not the speed or

  2023cites this paper
• Identification of Novel, Potent, and Selective Compounds against Malaria Using Glideosomal-Associated Protein 50 as a Drug Target

  2023cites this paper
• De novo mapping of the apicomplexan Ca2+-responsive proteome

  2022cites this paper
• Identification and characterization of GAP50 binders with the goal to identify novel antimalarials

  2022cites this paper
• Glycosylation Analysis of Feline Small Intestine Following Toxoplasma gondii Infection

  2022cites this paper
• Temporal and thermal profiling of the Toxoplasma proteome implicates parasite Protein Phosphatase 1 in the regulation of Ca2+-responsive pathways

  2022cites this paper
• Identification of a novel AP2 transcription factor in zygotes with an essential role in Plasmodium ookinete development

  2022cites this paper
• Disruption of Toxoplasma gondii-Induced Host Cell DNA Replication Is Dependent on Contact Inhibition and Host Cell Type

  2022cites this paper
• Marine gregarine genomes reveal the breadth of apicomplexan diversity with a partially conserved glideosome machinery

  2022cites this paper
• An apical protein, Pcr2, is required for persistent movement by the human parasite Toxoplasma gondii

  2022cites this paper
• Dominique Soldati-Favre: Bringing Toxoplasma gondii to the Molecular World

  2022cites this paper
• Marine gregarine genomes reveal the breadth of apicomplexan diversity and provide new insights on gliding motility

  2021cites this paper
• Cellular Mechanisms of Raphid Diatom Gliding

  2021cites this paper
• Identification and characterisation of splicing regulators in Toxoplasma gondii

  2021cites this paper
• Glideosomal GAP50 binders that inhibit invasion and restrict malaria parasite in vitro and in vivo

  2021cites this paper
• The Dynamic Roles of the Inner Membrane Complex in the Multiple Stages of the Malaria Parasite

  2021cites this paper
• Transcriptional modification of host cells harboring Toxoplasma gondii bradyzoites prevents IFN gamma-mediated cell death.

  2021cites this paper
• The Basal Complex Protein PfMORN1 Is Not Required for Asexual Replication of Plasmodium falciparum

  2021cites this paper
• Aminoglycerophospholipid flipping and P4-ATPases in Toxoplasma gondii

  2021influential citation
• A functionally divergent transcription elongation factor 1‐like protein in Toxoplasma gondii

  2021cites this paper
• Nanos gigantium humeris insidentes: old papers informing new research into Toxoplasma gondii

  2021cites this paper
• The Modular Circuitry of Apicomplexan Cell Division Plasticity

  2021cites this paper
• Identification of novel inner membrane complex and apical annuli proteins of the malaria parasite Plasmodium falciparum

  2021cites this paper
• TgZFP2 is a novel zinc finger protein involved in coordinating mitosis and budding in Toxoplasma

  2020cites this paper
• Blocking Palmitoylation of Toxoplasma gondii Myosin Light Chain 1 Disrupts Glideosome Composition but Has Little Impact on Parasite Motility

  2020cites this paper
• Estudio de la miosina B de Plasmodium falciparum y su posible papel en la invasión del parásito al glóbulo rojo

  2020cites this paper
• Fussing About Fission: Defining Variety Among Mainstream and Exotic Apicomplexan Cell Division Modes

  2020influential citation
• Calcium signaling through a transient receptor channel is important for Toxoplasma gondii growth

  2020cites this paper
• Toxoplasma secretory proteins and their roles in parasite cell cycle and infection

  2020cites this paper
• What a Difference 30 Years Makes! A Perspective on Changes in Research Methodologies Used to Study Toxoplasma gondii.

  2020cites this paper
• Proteomics and posttranslational protein modifications in Toxoplasma gondii

  2020cites this paper
• The Toxoplasma cytoskeleton: structures, proteins, and processes

  2020cites this paper
• Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion

  2019cites this paper
• Structural role of essential light chains in the apicomplexan glideosome

  2019cites this paper
• Alveolar proteins stabilize cortical microtubules in Toxoplasma gondii

  2019cites this paper
• Inner membrane complex 1l protein of Plasmodium falciparum links membrane lipids with cytoskeletal element 'actin' and its associated motor 'myosin'.

  2019cites this paper
• Phosphatidic acid governs natural egress in Toxoplasma gondii via a guanylate cyclase receptor platform

  2019cites this paper
• Caractérisation de TgZFP2, une nouvelle protéine à motif en doigt de zinc impliquée dans la coordination de la progression du cycle cellulaire du parasite apicomplexe Toxoplasma gondii

  2019cites this paper
• A highly dynamic F-actin network regulates transport and recycling of micronemes in Toxoplasma gondii vacuoles

  2019cites this paper
• Toxoplasma gondii bradyzoites induce transcriptional changes to host cells and prevent IFNγ-mediated cell death

  2019cites this paper
• Centrin2 from the human parasite Toxoplasma gondii is required for its invasion and intracellular replication

  2019influential citation
• An essential contractile ring protein controls cell division in Plasmodium falciparum

  2019cites this paper
• An apically located hybrid guanylate cyclase–ATPase is critical for the initiation of Ca2+ signaling and motility in Toxoplasma gondii

  2019cites this paper
• An unusual and vital protein with guanylate cyclase and P4-ATPase domains in a pathogenic protist

  2019influential citation
• An unusual and physiologically vital protein with guanylate cyclase and P-type ATPase like domain in a pathogenic protist

  2018influential citation
• TgCentrin2 is required for invasion and replication in the human parasite Toxoplasma gondii

  2018cites this paper
• Aspartyl Protease 5 Matures Dense Granule Proteins That Reside at the Host-Parasite Interface in Toxoplasma gondii

  2018cites this paper
• The Actinomyosin Motor Drives Malaria Parasite Red Blood Cell Invasion but Not Egress

  2018cites this paper
• Structural insights into a Plasmodium falciparum IMC1 protein using bioinformatics tools

  2018cites this paper
• Protein kinase A negatively regulates Ca2+ signalling in Toxoplasma gondii

  2018cites this paper
• Mitochondrial division in T. gondii: analysis of the Dynamin-related protein C and of its putative interactors

  2018influential citation
• Aspartyl Protease 5 matures virulence factors found at the host-parasite interface in Toxoplasma gondii

  2018influential citation
• Post‐translational modifications as key regulators of apicomplexan biology: insights from proteome‐wide studies

  2018cites this paper
• Identification and characterization of invasion-related proteins of the malaria parasite Plasmodium falciparum (Welch, 1892)

  2018cites this paper
• How does Toxoplama gondii invade host cells?

  2018cites this paper
• Etude fonctionnelle et structurale de protéines impliquées dans l'invasion des cellules hépatocytaires par les sporozoïtes de Plasmodium

  2017cites this paper
• Direct measurement of cortical force generation and polarization in a living parasite

  2017cites this paper
• Research advances in interactions related to Toxoplasma gondii microneme proteins.

  2017cites this paper
• Compositional and expression analyses of the glideosome during the Plasmodium life cycle reveal an additional myosin light chain required for maximum motility

  2017influential citation
• The Molecular Basis of Erythrocyte Invasion by Malaria Parasites.

  2017cites this paper
• Gliding motility powers invasion and egress in Apicomplexa

  2017cites this paper
• Structure-function studies of class I aldolases - exploring novel activities : mechanism, moonlighting, and inhibition

  2017cites this paper
• Disrupting assembly of the inner membrane complex blocks Plasmodium falciparum sexual stage development

  2017cites this paper
• The dynamic nature and functions of actin in Toxoplasma gondii

  2017cites this paper
• Localization and characterization of novel pellicle protein SPM3 in Toxoplasma gondii

  2017cites this paper
• Functions of myosin motors tailored for parasitism.

  2017cites this paper
• Crosstalk between PKA and PKG controls pH‐dependent host cell egress of Toxoplasma gondii

  2017cites this paper
• An essential malaria protein defines the architecture of blood-stage and transmission-stage parasites

  2016cites this paper
• A MORN1‐associated HAD phosphatase in the basal complex is essential for Toxoplasma gondii daughter budding

  2016cites this paper
• Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog

  2016cites this paper
• Reassessing the mechanics of parasite motility and host-cell invasion

  2016cites this paper
• An ensemble of specifically targeted proteins stabilizes cortical microtubules in the human parasite Toxoplasma gondii

  2016cites this paper
• Gliding Associated Proteins Play Essential Roles during the Formation of the Inner Membrane Complex of Toxoplasma gondii

  2016influential citation
• Interrogating the Plasmodium Sporozoite Surface: Identification of Surface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP by Mass Spectrometry-Based Proteomics

  2016influential citation
• Apicoplast fatty acid synthesis is essential for pellicle formation at the end of cytokinesis in Toxoplasma gondii

  2016influential citation
• Glideosome of Apicomplexans as a Drug Target

  2016cites this paper
• Gliding motility in apicomplexan parasites.

  2015cites this paper