Enhanced Solubilization of Class B Radical S-Adenosylmethionine Methylases by Improved Cobalamin Uptake in Escherichia coli.

No abstract is available for this paper.

• Publication year

  2018

• Venue

  Biochemistry

• Publication date

  2018-01-03

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1021/acs.biochem.7b01205PMID 29298049PMCID PMC5941297
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Efficient methylation of C2 in l-tryptophan by the cobalamin-dependent radical S-adenosylmethionine methylase TsrM requires an unmodified N1 amine

  2017cited by this paper
• Biosynthesis of Branched Alkoxy Groups: Iterative Methyl Group Alkylation by a Cobalamin-Dependent Radical SAM Enzyme.

  2017cited by this paper
• Methylcobalamin-Dependent Radical SAM C-Methyltransferase Fom3 Recognizes Cytidylyl-2-hydroxyethylphosphonate and Catalyzes the Nonstereoselective C-Methylation in Fosfomycin Biosynthesis.

  2017cited by this paper
• NosN, a Radical S-Adenosylmethionine Methylase, Catalyzes Both C1 Transfer and Formation of the Ester Linkage of the Side-Ring System during the Biosynthesis of Nosiheptide.

  2017cited by this paper
• A B12-dependent radical SAM enzyme involved in Oxetanocin-A biosynthesis

  2017cited by this paper
• TsrM as a Model for Purifying and Characterizing Cobalamin-Dependent Radical S-Adenosylmethionine Methylases.

  2017cited by this paper
• Vitamin B12 in the spotlight again.

  2017cited by this paper
• The Catalytic Mechanism of the Class C Radical S-Adenosylmethionine Methyltransferase NosN.

  2017cited by this paper
• The B12-Radical SAM Enzyme PoyC Catalyzes Valine Cβ-Methylation during Polytheonamide Biosynthesis

  2016cited by this paper
• Radical new paradigm for heme degradation in Escherichia coli O157:H7

  2016cited by this paper
• Polytheonamide biosynthesis showcasing the metabolic potential of sponge-associated uncultivated 'Entotheonella' bacteria.

  2016cited by this paper
• Maturation of nitrogenase cofactor-the role of a class E radical SAM methyltransferase NifB.

  2016cited by this paper
• Spectroscopic and Electrochemical Characterization of the Iron-Sulfur and Cobalamin Cofactors of TsrM, an Unusual Radical S-Adenosylmethionine Methylase.

  2016cited by this paper
• Crystallographic capture of a radical S-adenosylmethionine enzyme in the act of modifying tRNA

  2016cited by this paper
• Biochemical and Spectroscopic Studies of Epoxyqueuosine Reductase: A Novel Iron-Sulfur Cluster- and Cobalamin-Containing Protein Involved in the Biosynthesis of Queuosine.

  2015cited by this paper
• Consecutive radical S-adenosylmethionine methylations form the ethyl side chain in thienamycin biosynthesis

  2015cited by this paper
• Mechanistic Diversity of Radical S-Adenosylmethionine (SAM)-dependent Methylation*

  2014cited by this paper
• Characterization of a Cross-Linked Protein–Nucleic Acid Substrate Radical in the Reaction Catalyzed by RlmN

  2014cited by this paper
• Initial characterization of Fom3 from Streptomyces wedmorensis: The methyltransferase in fosfomycin biosynthesis.

  2014cited by this paper
• Structure of AMP-PNP–bound BtuCD and mechanism of ATP-powered vitamin B12 transport by BtuCD–F

  2014cited by this paper
• Author Manuscript

  2013cited by this paper
• The Structure–Function Linkage Database

  2013cited by this paper
• A Substrate Radical Intermediate in Catalysis by the Antibiotic Resistance Protein Cfr

  2013cited by this paper
• Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

  2012cited by this paper
• Metagenome Mining Reveals Polytheonamides as Posttranslationally Modified Ribosomal Peptides

  2012cited by this paper
• RlmN and AtsB as models for the overproduction and characterization of radical SAM proteins.

  2012cited by this paper
• Thiostrepton tryptophan methyltransferase expands the chemistry of radical SAM enzymes.

  2012cited by this paper
• The Escherichia coli RlmN methyltransferase is a dual-specificity enzyme that modifies both rRNA and tRNA and controls translational accuracy.

  2012cited by this paper
• A Radically Different Mechanism for S-Adenosylmethionine–Dependent Methyltransferases

  2011cited by this paper
• Radical-Mediated Enzymatic Methylation: A Tale of Two SAMS

  2011cited by this paper
• In vitro phosphinate methylation by PhpK from Kitasatospora phosalacinea.

  2011cited by this paper
• Effect of Chemical Chaperones in Improving the Solubility of Recombinant Proteins in Escherichia coli

  2011cited by this paper
• RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA.

  2010cited by this paper
• Escherichia coli allows efficient modular incorporation of newly isolated quinomycin biosynthetic enzyme into echinomycin biosynthetic pathway for rational design and synthesis of potent antibiotic unnatural natural product.

  2009cited by this paper
• Unusual chemistry in the biosynthesis of the antibiotic chondrochlorens.

  2009cited by this paper
• Thiostrepton biosynthesis: prototype for a new family of bacteriocins.

  2009cited by this paper
• Gene inactivation study of gntE reveals its role in the first step of pseudotrisaccharide modifications in gentamicin biosynthesis.

  2008cited by this paper
• Chlorophyll biosynthesis in bacteria: the origins of structural and functional diversity.

  2007cited by this paper
• Biosynthesis of clorobiocin: investigation of the transfer and methylation of the pyrrolyl-2-carboxyl moiety

  2007cited by this paper
• Cloning of the Pactamycin Biosynthetic Gene Cluster and Characterization of a Crucial Glycosyltransferase Prior to a Unique Cyclopentane Ring Formation

  2007cited by this paper
• A streamlined metabolic pathway for the biosynthesis of moenomycin A.

  2007cited by this paper
• New insight into the mechanism of methyl transfer during the biosynthesis of fosfomycin.

  2007cited by this paper
• Heterologous production of fosfomycin and identification of the minimal biosynthetic gene cluster.

  2006cited by this paper
• Outer Membrane Active Transport: Structure of the BtuB:TonB Complex

  2006cited by this paper
• Protein production by auto-induction in high density shaking cultures.

  2005cited by this paper
• Measurement of energetics of conformational change in cobalamin-dependent methionine synthase.

  2004cited by this paper
• The biosynthetic gene cluster for the beta-lactam carbapenem thienamycin in Streptomyces cattleya.

  2003cited by this paper
• The E. coli BtuCD Structure: A Framework for ABC Transporter Architecture and Mechanism

  2002cited by this paper
• Identification of the Periplasmic Cobalamin-Binding Protein BtuF of Escherichia coli

  2002cited by this paper
• Adenosylcobalamin inhibits ribosome binding to btuB RNA.

  2000cited by this paper
• Molecular characterization and analysis of the biosynthetic gene cluster for the antitumor antibiotic mitomycin C from Streptomyces lavendulae NRRL 2564.

  1999cited by this paper
• Initiation of translation in prokaryotes and eukaryotes.

  1999cited by this paper
• Cobalamin (coenzyme B12): synthesis and biological significance.

  1996cited by this paper
• Sequence of a P-methyltransferase-encoding gene isolated from a bialaphos-producing Streptomyces hygroscopicus.

  1995cited by this paper
• Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant.

  1995cited by this paper
• Nucleotide sequence of fortimicin KL1 methyltransferase gene isolated from Micromonospora olivasterospora, and comparison of its deduced amino acid sequence with those of methyltransferases involved in the biosynthesis of bialaphos and fosfomycin.

  1995cited by this paper
• Cloning and nucleotide sequence of fosfomycin biosynthetic genes of Streptomyces wedmorensis

  1995cited by this paper
• Self cloning in Micromonospora olivasterospora of fms genes for fortimicin A (astromicin) biosynthesis

  1992cited by this paper
• Studies on the biosynthesis of fosfomycin. 4. The biosynthetic origin of the methyl group of fosfomycin.

  1992cited by this paper
• Scanning model for translational reinitiation in eubacteria.

  1990cited by this paper
• Point mutations in a conserved region (TonB box) of Escherichia coli outer membrane protein BtuB affect vitamin B12 transport

  1989cited by this paper
• Altered cobalamin metabolism in Escherichia coli btuR mutants affects btuB gene regulation

  1989cited by this paper
• Vitamin B12 transport inEscherichia coli K12 does not require thebtuE gene of thebtuCED operon

  1989cited by this paper
• Identification of the btuCED polypeptides and evidence for their role in vitamin B12 transport in Escherichia coli

  1986cited by this paper
• Reduced activity of TonB-dependent functions in strains of Escherichia coli

  1986cited by this paper
• Transport of vitamin B12 in Escherichia coli: cloning of the btuCD region

  1985cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• The mechanism of action of ethanolamine ammonia-lyase, a B-12-dependent enzyme. The participation of paramagnetic species in the catalytic deamination of 2-aminopropanol.

  1974cited by this paper
• Isolation of a protein containing tightly bound 5-methoxybenzimidazolylcobamide (factor 3m) from Clostridium thermoaceticum.

  1973cited by this paper
• Experiments in molecular genetics

  1972cited by this paper
• Regulation of the terminal reactions in methionine biosynthesis by vitamin B 12 and methionine.

  1972cited by this paper
• MUTANTS OF ESCHERICHIA COLI REQUIRING METHIONINE OR VITAMIN B12

  1950cited by this paper

• Structural basis for iterative methylation by a cobalamin-dependent radical S-adenosylmethionine enzyme in cystobactamids biosynthesis

  2026cites this paper
• Novel chemistry and structural perspectives in vitamin B12-dependent radical SAM enzymes.

  2026cites this paper
• Biosynthese der Biphenomycine, einer Familie potenter Antibiotika

  2025cites this paper
• Biosynthesis of the Biphenomycin Family of Potent Antibiotics

  2025cites this paper
• Dps binds and protects DNA in starved Escherichia coli with minimal effect on chromosome accessibility, dynamics and organisation

  2025cites this paper
• Engineering biology and chemical approaches to the construction of vitamin B12 analogues and antivitamins B12 as probes and therapeutic agents.

  2025cites this paper
• Biosynthesis of Biphenomycin-like Macrocyclic Peptides by Formation and Cross-Linking of Ortho-Tyrosines

  2025cites this paper
• Radical Fluoromethylation Enabled by Cobalamin-Dependent Radical SAM Enzymes

  2025cites this paper
• Peptide surfactants with post-translational C-methylations that promote bacterial development

  2025cites this paper
• Improved methyl supply for 5-methyltetrahydrofolate production in E. coli through a novel C1 transfer pathway

  2025cites this paper
• Biosynthesis of Macrocyclic Peptides by Formation and Crosslinking of ortho-Tyrosines

  2025cites this paper
• Engineering archaeal membrane‐spanning lipid GDGT biosynthesis in bacteria: Implications for early life membrane transformations

  2025influential citation
• Structural Basis for Iterative Methylation by a Cobalamin-dependent Radical S-Adenosylmethionine Enzyme in Cystobactamids Biosynthesis

  2025cites this paper
• Chemical richness and diversity of uncultivated ‘Entotheonella’ symbionts in marine sponges

  2025cites this paper
• The cobalamin-binding domain of cobalamin-dependent radical S-adenosylmethionine enzymes: Familiarity in unfamiliar places.

  2025cites this paper
• Structural Evidence for DUF512 as a Radical S-Adenosylmethionine Cobalamin-Binding Domain

  2024cites this paper
• From mec cassette to rdhA: a key Dehalobacter genomic neighborhood in a chloroform and dichloromethane–transforming microbial consortium

  2024cites this paper
• Cobalamin-dependent radical S-adenosyl-L-methionine protein functions with a partner to successively methylate tricyclic indole alkaloid for chuangxinmycin maturation and derivatization

  2024cites this paper
• S-adenosyl-L-methionine is the unexpected methyl donor for the methylation of mercury by the membrane-associated HgcAB complex

  2024cites this paper
• MecE, MecB, and MecC proteins orchestrate methyl group transfer during dichloromethane fermentation

  2024cites this paper
• Adenosyl derivatives as a potential inhibitors of NS3 protease of Japanese encephalitis virus (JEV): In silico molecular insight into therapeutic discovery

  2024cites this paper
• Deciphering reductive dehalogenase specificity through targeted mutagenesis of chloroalkane reductases

  2024cites this paper
• Contrasting Kinetics of Highly Similar Chloroalkane Reductive Dehalogenases

  2024cites this paper
• Accessing and exploring the unusual chemistry by radical SAM-RiPP enzymes.

  2024cites this paper
• Harnessing iron‑sulfur enzymes for synthetic biology.

  2024cites this paper
• Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli

  2024influential citation
• The Rhodium Analogue of Coenzyme B12 as an Anti-Photoregulatory Ligand Inhibiting Bacterial CarH Photoreceptors.

  2024cites this paper
• Widespread Peptide Surfactants with Post-translational C-methylations Promote Bacterial Development

  2024cites this paper
• Sustainable B12-Dependent Dehalogenation of Organohalides in E. coli.

  2024cites this paper
• Changing Fates of the Substrate Radicals Generated in the Active Sites of the B12-Dependent Radical SAM Enzymes OxsB and AlsB.

  2023cites this paper
• Efficient NADPH-dependent dehalogenation afforded by a self-sufficient reductive dehalogenase

  2023cites this paper
• Docking and MM study of non-structural protein (NS5) of Japanese Encephalitis Virus (JEV) with some derivatives of adenosyl

  2023cites this paper
• B12-dependent radical SAM enzymes: Ever expanding structural and mechanistic diversity.

  2023cites this paper
• Biosynthesis of macrocyclic peptides with C-terminal β-amino-α-keto acid groups by three different metalloenzymes

  2023cites this paper
• The new epoch of structural insights into radical SAM enzymology.

  2023cites this paper
• Characterization of the cobalamin-dependent radical S-adenosyl-l-methionine enzyme C-methyltransferase Fom3 in fosfomycin biosynthesis.

  2022cites this paper
• Functional Diversity of HemN-like Proteins

  2022cites this paper
• Cobalamin-Dependent Radical S-Adenosylmethionine Enzymes: Capitalizing on Old Motifs for New Functions

  2022cites this paper
• Structural characterization of cobalamin-dependent radical S-adenosylmethionine methylases.

  2022cites this paper
• Structure of a B12-dependent radical SAM enzyme in carbapenem biosynthesis

  2022cites this paper
• Studies of GenK and OxsB, two B12-dependent radical SAM enzymes involved in natural product biosynthesis.

  2022cites this paper
• Purification and structural elucidation of a cobalamin-dependent radical SAM enzyme.

  2022cites this paper
• Radical SAM Enzymes Involved in Tetrapyrrole Biosynthesis and Insertion

  2022cites this paper
• Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host

  2022cites this paper
• Exploring the biosynthetic capacity of TsrM, a B12-dependent Radical SAM Enzyme Catalyzing Non-radical Reactions.

  2022cites this paper
• The Atypical Cobalamin-Dependent S-Adenosyl-l-Methionine Nonradical Methylase TsrM and Its Radical Counterparts

  2022cites this paper
• A Cobalamin‐Dependent Radical SAM Enzyme Catalyzes the Unique Cα‐Methylation of Glutamine in Methyl‐Coenzyme M Reductase

  2022cites this paper
• Purification and characterization of sequential cobalamin-dependent radical SAM methylases ThnK and TokK in carbapenem β-lactam antibiotic biosynthesis.

  2022cites this paper
• Biosynthesis of 3-thia-α-amino acids on a carrier peptide

  2022cites this paper
• Discovery, structure and mechanism of a tetraether lipid synthase

  2022cites this paper
• ThnL, a B12-dependent radical S-adenosylmethionine enzyme, catalyzes thioether bond formation in carbapenem biosynthesis

  2022cites this paper
• Twenty Years of Radical SAM! The Genesis of the Superfamily

  2022cites this paper
• Overall Retention of Methyl Stereochemistry during B12-Dependent Radical SAM Methyl Transfer in Fosfomycin Biosynthesis

  2021cites this paper
• Resolving phylogenetic and biochemical barriers to functional expression of heterologous iron-sulphur cluster enzymes

  2021cites this paper
• Biosynthesis of Oxetanocin-A Includes a B12-Dependent Radical SAM Enzyme That Can Catalyze both Oxidative Ring Contraction and the Demethylation of SAM.

  2021cites this paper
• Heterologous Expression of Active Dehalobacter Respiratory Reductive Dehalogenases in Escherichia coli

  2021influential citation
• Evolution of Methods for the Study of Cobalamin-Dependent Radical SAM Enzymes

  2021cites this paper
• Towards a Synthetic Biology Toolset for Metallocluster Enzymes in Biosynthetic Pathways: What We Know and What We Need

  2021cites this paper
• Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications

  2021cites this paper
• Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia coli

  2021cites this paper
• Structural basis for carbapenam C-alkylation by TokK, a B12-dependent radical SAM enzyme

  2021cites this paper
• A roadmap for metagenomic enzyme discovery

  2021cites this paper
• Supplemental

  2020cites this paper
• Structure determination of the HgcAB complex using metagenome sequence data: insights into microbial mercury methylation

  2020cites this paper
• Reductive Dehalogenases

  2020cites this paper
• Structural basis for non-radical catalysis by TsrM, a radical-SAM methylase

  2020cites this paper
• A Radical Exploration of the Cobalamin-Dependent Radical SAM Enzyme CysS

  2020cites this paper
• Heterologous expression of cobalamin dependent class-III enzymes

  2020cites this paper
• Structure determination of the HgcAB complex using metagenome sequence data: insights into microbial mercury methylation

  2020cites this paper
• Lifetimes of the Aglycone Substrates of Specifier Proteins, the Autonomous Iron Enzymes that Dictate the Products of the Glucosinolate-Myrosinase Defense System in Brassica Plants.

  2020cites this paper
• C-Methylation of S-adenosyl-L-Methionine Occurs Prior to Cyclopropanation in the Biosynthesis of 1-Amino-2-Methylcyclopropanecarboxylic Acid (Norcoronamic Acid) in a Bacterium

  2020cites this paper
• Mechanistic studies on CysS - a Vitamin B12-dependent radical SAM methyltransferase involved in the biosynthesis of the t-butyl group of cystobactamid.

  2020cites this paper
• Radical S-Adenosylmethionine Methylases

  2020cites this paper
• The Chemistry and Structural Enzymology of RiPP-Modifying Radical SAM Metalloenzymes

  2020cites this paper
• Structure and Biosynthesis of Proteusin RiPP Natural Products

  2020cites this paper
• Methanogenesis marker protein 10 (Mmp10) from Methanosarcina acetivorans is a radical S-adenosylmethionine methylase that unexpectedly requires cobalamin

  2019cites this paper
• Methylations in complex carbapenem biosynthesis are catalyzed by a single cobalamin-dependent radical S-adenosylmethionine enzyme.

  2019cites this paper
• Genome mining- and synthetic biology-enabled production of hypermodified peptides

  2019cites this paper
• Understanding the role of electron donors in the reaction catalyzed by Tsrm, a cobalamin-dependent radical S-adenosylmethionine methylase

  2019cites this paper
• Producing high-value chemicals in Escherichia coli through synthetic biology and metabolic Engineering

  2019cites this paper
• Stereospecific Radical-Mediated B12-Dependent Methyl Transfer by the Fosfomycin Biosynthesis Enzyme Fom3.

  2018cites this paper
• Stereochemical and Mechanistic Investigation of the Reaction Catalyzed by Fom3 from Streptomyces fradiae, a Cobalamin-Dependent Radical S-Adenosylmethionine Methylase.

  2018cites this paper
• A Rich Man, Poor Man Story of S-Adenosylmethionine and Cobalamin Revisited.

  2018cites this paper
• Aerobic Enzymes and Their Radical SAM Enzyme Counterparts in Tetrapyrrole Pathways.

  2018cites this paper
• Iterative Methylations Resulting in the Biosynthesis of the t-Butyl Group Catalyzed by a B12-Dependent Radical SAM Enzyme in Cystobactamid Biosynthesis.

  2018cites this paper
• Cobalamin-dependent radical S-adenosyl-l-methionine enzymes in natural product biosynthesis.

  2018cites this paper