MicroRNA-7 Inhibits the Growth of Human Non-Small Cell Lung Cancer A549 Cells through Targeting BCL-2

MicroRNAs(miRNAs) are emerging as important regulators in tumorigenesis. Increasing evidences have indicated microRNA-7(miR-7) to be a potential tumor suppressor in several human cancers. However, only a limited number of target genes have been identified so far and its biological function in Non-Small Cell Lung Cancer (NSCLC) remains to be further elucidated. In the present study, we observed a reduction of miR-7 level in NSCLC cell lines. Overexpression of miR-7 not only suppressed NSCLC A549 cells proliferation, induced cell apoptosis and inhibited cell migration in vitro, but also reduced tumorigenicity in vivo. Bioinformatics predictions revealed a potential binding site of miR-7 on 3'UTR of BCL-2 and it was further confirmed by luciferase assay. Moreover, subsequent experiments showed that BCL-2 was downregulated by miR-7 at both transcriptional and translational levels. These results suggest that miR-7 regulates the expression of BCL-2 through direct 3'UTR interactions. Therefore, we postulate BCL-2 to be a novel target possibly involved in miR-7-mediated growth suppression and apoptosis of A549 cells. These findings may provide a basic rationale for the use of miR-7 in the treatment of NSCLC.

• Publication year

  2011

• Venue

  International Journal on Biological Sciences

• Publication date

  2011-07-04

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.7150/IJBS.7.805PMID 21750649PMCID 3133888
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Dysregulation of microRNA-34a expression causes drug-resistance to 5-FU in human colon cancer DLD-1 cells.

  2011cited by this paper
• miRNAs in human cancer

  2011cited by this paper
• MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell Lines

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• Modulation of Bcl-2-related protein expression in pancreatic beta cells by pro-inflammatory cytokines and its dependence on the antioxidative defense status.

  2011cited by this paper
• miRNA-7 attenuation in Schwannoma tumors stimulates growth by upregulating three oncogenic signaling pathways.

  2011influential reference
• Functional analysis of microRNAs in human hepatocellular cancer stem cells

  2011cited by this paper
• Clinicopathological and Prognostic Value of MicroRNA-21 and MicroRNA-155 in Colorectal Cancer

  2011cited by this paper
• MicroRNA let-7a represses chemoresistance and tumourigenicity in head and neck cancer via stem-like properties ablation.

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• Mutator activity induced by microRNA-155 (miR-155) links inflammation and cancer

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• Micro(mi)RNA expression profile of breast cancer epithelial cells treated with the anti-diabetic drug metformin: Induction of the tumor suppressor miRNA let-7a and suppression of the TGFβ-induced oncomiR miRNA-181a

  2011cited by this paper
• miRNA: small molecules as potential novel biomarkers in cancer.

  2010cited by this paper
• High expression of BCL-2 predicts favorable outcome in non-small cell lung cancer patients with non squamous histology

  2010cited by this paper
• Gene regulation through RNA editing.

  2010cited by this paper
• MicroRNA in lung cancer

  2010cited by this paper
• Targeting Bcl-2-mediated cell death as a novel therapy in pancreatic cancer.

  2010cited by this paper
• MicroRNA-155 functions as an OncomiR in breast cancer by targeting the suppressor of cytokine signaling 1 gene.

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• Viral Oncogenes, Noncoding RNAs, and RNA Splicing in Human Tumor Viruses

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• [Effect of combined therapy with Chinese drug and narrow broad ultraviolet B on Bcl-2, caspase-3 and survivin in skin lesion with psoriasis vulgaris].

  2010cited by this paper
• MicroRNA-7 targets IGF1R (insulin-like growth factor 1 receptor) in tongue squamous cell carcinoma cells.

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• Targeting microRNAs in cancer: rationale, strategies and challenges

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  2008cited by this paper
• microRNA-7 inhibits the epidermal growth factor receptor and the Akt pathway and is down-regulated in glioblastoma.

  2008influential reference
• Apoptosis induction by Bcl-2 proteins independent of the BH3 domain.

  2008cited by this paper
• MicroRNA Detection and Target Prediction: Integration of Computational and Experimental Approaches

  2007cited by this paper
• MicroRNA in lung cancer.

  2006cited by this paper
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  2004cited by this paper
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  2001cited by this paper
• Bcl-2 family proteins as targets for anticancer drug design

  2000cited by this paper

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  2021cites this paper
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• MicroRNAs in non‐small cell lung cancer: Gene regulation, impact on cancer cellular processes, and therapeutic potential

  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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• OTT_A_221005 8737..8750

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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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  2018cites this paper
• Inhibitory effect of 11-carbonyl-beta-boswellic acid on non-small cell lung cancer H446 cells.

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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017influential citation
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  2017cites this paper
• Significance and Function of MicroRNA-7 in Oesophageal Squamous Cell Carcinoma.

  2017cites this paper