Comparison of methods of high-pressure freezing and automated freeze-substitution of suspension cells combined with LR White embedding

No abstract is available for this paper.

• Publication year

  2010

• Venue

  Histochemistry and Cell Biology

• Publication date

  2010-11-10

• Fields of study

  Biology, Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1007/s00418-010-0757-zPMID 21063722
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Colloidal Gold: Principles, Methods, and Applications

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  2010influential reference
• High‐pressure freezing of spermiogenic nuclei supports a dynamic chromatin model for the histone‐to‐protamine transition

  2009cited by this paper
• [Sensitization to acrylates caused by artificial acrylic nails: review of 15 cases].

  2008cited by this paper
• Close-to-native ultrastructural preservation by high pressure freezing.

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• Freeze‐substitution: the addition of water to polar solvents enhances the retention of structure and acts at temperatures around –60°C

  2008influential reference
• Sensibilizacin a acrilatos por uas artificiales acrlicas. Revisin de 15 casos

  2008cited by this paper
• Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells

  2008influential reference
• Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution

  2008influential reference
• Contact allergy to acrylates/methacrylates in the acrylate and nail acrylics series in southern Sweden: simultaneous positive patch test reaction patterns and possible screening allergens

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• Rapid freeze‐substitution preserves membranes in high‐pressure frozen tissue culture cells

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• Cryopreparation methods for electron microscopy of selected model systems.

  2007influential reference
• Immunogold Labeling of Cryosections from High‐Pressure Frozen Cells

  2007cited by this paper
• Subcellular localization of fumarase in mammalian cells and tissues

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• Sensitization to acrylates is a common adverse reaction to artificial fingernails

  2007cited by this paper
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  2007cited by this paper
• Epoxy resin as fixative during freeze-substitution.

  2005cited by this paper
• New insights in thylakoid membrane organization.

  2005influential reference
• Comparison of infrastructure of Germinating Pea Leaves Prepared by High-Pressure Freezing-Freeze Substitution and Conventional Chemical Fixation

  2005cited by this paper
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  2004cited by this paper
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  2004influential reference
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  2004influential reference
• Influence of aldehyde fixation on the morphology of endosomes and lysosomes: quantitative analysis and electron tomography

  2003cited by this paper
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  2003cited by this paper
• Direct attachment of cell suspensions to high‐pressure freezing specimen planchettes

  2003cited by this paper
• Freeze‐substitution protocols for improved visualization of membranes in high‐pressure frozen samples

  2003influential reference
• The use of filter membranes for high‐pressure freezing of cell monolayers

  2003influential reference
• Of plants and other pets: practical aspects of freeze‐substitution and resin embedding

  2003cited by this paper
• Freeze substitution of high‐pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water

  2002influential reference
• Immunolocalization of long-chain acyl-CoAs in plant cells.

  2002cited by this paper
• Developing Seeds of Arabidopsis Store Different Minerals in Two Types of Vacuoles and in the Endoplasmic Reticulum Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010486.

  2002influential reference
• Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze‐substitution

  2001influential reference
• A new approach for cryofixation by high‐pressure freezing

  2001cited by this paper
• Cryosubstitution of frozen biological specimens in electron microscopy: use and application as an alternative to chemical fixation.

  2001cited by this paper
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  1999influential reference
• Ultrastructure of chloroplast protuberances in rice leaves preserved by high-pressure freezing

  1999cited by this paper
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  1999cited by this paper
• Tubules of the trans Golgi apparatus visualized by immunoelectron microscopy

  1998cited by this paper
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  1998cited by this paper
• Immuno-cross-reactivity of CUT-1 and cuticlin epitopes between ascaris lumbricoides, Caenorhabditis elegans, and Heterorhabditis.

  1998cited by this paper
• High‐pressure freezing and freeze‐substitution of native rat brain: Suitability for preservation and immunoelectron microscopic localization of myelin glycolipids

  1998cited by this paper
• High‐pressure freezing for immunocytochemistry

  1998influential reference
• 10 years of patch testing with the (meth)acrylate series

  1997cited by this paper
• Profilin revealed in pollen nuclei: immuno-electron microscopy of high-pressure frozen Ledebouria socialis Roth (Hyacinthaceae)

  1997cited by this paper
• Ultrastructural immunolocalization of polyamines in HeLa cells subjected to fast-freezing fixation and freeze substitution

  1997cited by this paper
• Improved Procedure for Immunogold Electron Microscopy: Rapid-Freeze Substitution with Absolute Acetone

  1997cited by this paper
• Light damage in rod outer segments: the effects of fixation on ultrastructural alterations.

  1996cited by this paper
• Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants

  1995cited by this paper
• High-pressure freeze fixation reveals novel features during ontogenesis of the vegetative cell in Ledebouria pollen: an ultrastructural and cytochemical study.

  1995influential reference
• Vitrification of articular cartilage by high‐pressure freezing

  1995cited by this paper
• Immunocytochemical localisation of actin and profilin in the generative cell of angiosperm pollen: TEM studies on high-pressure frozen and freeze-substitutedLedebouria socialis Roth (Hyacinthaceae)

  1995cited by this paper
• High-density morphologies of ice in high-pressure frozen biological specimens.

  1994cited by this paper
• Subunits of forming pollen exine and Ubisch bodies as seen in freeze substitutedLedebouria socialis Roth (Hyacinthaceae)

  1994cited by this paper
• Effect of monensin on plant Golgi: re-examination of the monensin-induced changes in cisternal architecture and functional activities of the Golgi apparatus of sycamore suspension-cultured cells.

  1993cited by this paper
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  1993cited by this paper
• Cryofixation and cryosubstitution: a useful alternative in the analyses of cellular fine structure.

  1993cited by this paper
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  1992cited by this paper
• Quantitative immuno-gold labelling and ultrastructural preservation after cryofixation (combined with different freeze-substitution and embedding protocols) and after chemical fixation and cryosectioning

  1992cited by this paper
• Localization of heparan sulfate proteoglycan in basement membrane by side chain staining with cuprolinic blue as compared with core protein labeling with immunogold.

  1992cited by this paper
• High-pressure freezing of soybean nodules leads to an improved preservation of ultrastructure

  1992cited by this paper
• Artefacts and morphological changes during chemical fixation

  1992cited by this paper
• Freeze Substitution and Low-Temperature Embedding

  1992cited by this paper
• Cryosectioning of plant material frozen at high pressure

  1991cited by this paper
• Advantages of fast-freeze fixation followed by freeze-substitution for the preservation of cell integrity.

  1991cited by this paper
• Freeze‐substitution and the preservation of diffusible ions

  1991cited by this paper
• Some applications of cryosubstitution in ultrastructural studies of the cell nucleus

  1991cited by this paper
• The potential of cryofixation and freeze substitution: observations and theoretical considerations

  1991cited by this paper
• Contact dermatitis from acrylate and methacrylate compounds in Lowicryl® embedding media for electron microscopy

  1990cited by this paper
• Principles and techniques of electron microscopy

  1990cited by this paper
• Occupational risks of (meth)acrylate compounds in embedding media for electron microscopy

  1990cited by this paper
• Postembedding labeling on Lowicryl K4M tissue sections: detection and modification of cellular components.

  1989cited by this paper
• Immunogold labelling of actin on sections of freeze-substituted plant cells

  1989influential reference
• In situ localization of a foreign protein in transgenic plants by immunoelectron microscopy following high pressure freezing: freeze substitution and low temperature embedding

  1989influential reference
• 3 – Role of Tissue Processing in Colloidal Gold Methods

  1989cited by this paper
• Immuno-Gold Labeling in Cell Biology

  1989cited by this paper
• High pressure freezing comes of age.

  1989cited by this paper
• The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions.

  1987cited by this paper
• Developments of new Lowicryl® resins for embedding biological specimens at even lower temperatures

  1986cited by this paper
• Low Temperature Methods in Biological Electron Microscopy

  1985cited by this paper
• Extraction of lipids during freeze‐substitution of Acholeplasma laidlawii‐cells for electron microscopy

  1984cited by this paper
• The control of freeze-drying with deuterium oxide (D2O).

  1982cited by this paper
• Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.

  1981cited by this paper
• Principles and Techniques of Electron Microscopy: Biological Applications

  1975cited by this paper
• Practical methods in electron microscopy

  1973cited by this paper

• Immunoelectron microscopy: a comprehensive guide from sample preparation to high-resolution imaging

  2025cites this paper
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• Electron Microscopy Analysis of Membraneless Condensates in Plant Cells.

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• Arabidopsis HOPS subunit VPS41 carries out plant-specific roles in vacuolar transport and vegetative growth.

  2022cites this paper
• Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription

  2018influential citation
• Colocalization coefficients evaluating the distribution of molecular targets in microscopy methods based on pointed patterns

  2016influential citation
• UNIVERZITA KARLOVA V PRAZE PŘÍRODOVĚDECKÁ FAKULTA

  2015cites this paper
• Transmission Electron Microscopy of Biological Samples

  2015cites this paper
• Closer to the native state. Critical evaluation of cryo-techniques for Transmission Electron Microscopy: preparation of biological samples.

  2014cites this paper
• UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity

  2013cites this paper
• The Impact of Blanching and High-Pressure Pretreatments on Oil Uptake of Fried Potato Slices

  2012cites this paper
• Transmission electron microscopy in cell biology: sample preparation techniques and image information

  2012cites this paper
• Quantitative evaluation of freeze-substitution effects on preservation of nuclear antigens during preparation of biological samples for immunoelectron microscopy

  2012influential citation
• Histochemistry and cell biology: the annual review 2010

  2011cites this paper
• Annexin-A5 is fundamental for VDAC1-dependent mitochondrial Ca2+ homeostasis and determines the susceptibility to apoptosis.

  year unknowncites this paper