Rapid freeze‐substitution preserves membranes in high‐pressure frozen tissue culture cells

No abstract is available for this paper.

• Publication year

  2007

• Venue

  Journal of Microscopy

• Publication date

  2007-05-01

• Fields of study

  Biology, Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1111/j.1365-2818.2007.01767.xPMID 17444947
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Aclar discs: a versatile substrate for routine high‐pressure freezing of mammalian cell monolayers

  2006cited by this paper
• Impairment of Nuclear Pores in Bovine Herpesvirus 1-Infected MDBK Cells

  2005cited by this paper
• Epoxy resin as fixative during freeze-substitution.

  2005cited by this paper
• Transport of African Swine Fever Virus from Assembly Sites to the Plasma Membrane Is Dependent on Microtubules and Conventional Kinesin

  2004cited by this paper
• High‐pressure freezing of epithelial cells on sapphire coverslips

  2004cited by this paper
• Freeze‐substitution protocols for improved visualization of membranes in high‐pressure frozen samples

  2003cited by this paper
• Freeze substitution of high‐pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water

  2002cited by this paper
• Inaugural Article: Organellar relationships in the Golgi region of the pancreatic beta cell line, HIT-T15, visualized by high resolution electron tomography

  2001cited by this paper
• The Epithelial Integrin αvβ6 Is a Receptor for Foot-and-Mouth Disease Virus

  2000cited by this paper
• Cryopreparation provides new insight into the effects of brefeldin A on the structure of the HepG2 Golgi apparatus.

  2000cited by this paper
• The epithelial integrin alphavbeta6 is a receptor for foot-and-mouth disease virus.

  2000cited by this paper
• Ethane-freezing/methanol-fixation of cell monolayers: a procedure for improved preservation of structure and antigenicity for light and electron microscopies.

  1998cited by this paper
• High‐pressure freezing for immunocytochemistry

  1998cited by this paper
• African swine fever virus gene j13L encodes a 25-27 kDa virion protein with variable numbers of amino acid repeats.

  1995cited by this paper
• High‐pressure freezing of cell suspensions in cellulose capillary tubes

  1994cited by this paper
• Freeze Substitution and Low-Temperature Embedding

  1992cited by this paper
• Principles and Techniques of Electron Microscopy: Biological Applications

  1992cited by this paper
• Low-Temperature Microscopy and Analysis

  1992cited by this paper
• An appraisal of low‐temperature embedding by progressive lowering of temperature into Lowicryl HM20 for immunocytochemical studies

  1992cited by this paper
• Freeze‐substitution and the preservation of diffusible ions

  1991cited by this paper
• High pressure freezing comes of age.

  1989cited by this paper
• Titration of African swine fever (ASF) virus.

  1976cited by this paper
• Principles and Techniques of Electron Microscopy

  1975cited by this paper

• Molecular basis for cellular compartmentalization by an ancient membrane fission mechanism

  2025cites this paper
• Electron microscopy reveals saturated fatty acid-induced membrane defects in AdipoR2-depleted cells

  2025cites this paper
• Optimisation of freeze substitution protocols for the examination of malaria parasite structure by volumetric electron microscopy

  2025cites this paper
• Combining the CryoCapsule, HPM live µ and R221 conductive resin to track endosome from live imaging to electron microscopy using high-pressure freezing

  2025cites this paper
• LDO proteins and Vac8 form a vacuole-lipid droplet contact site to enable starvation-induced lipophagy in yeast.

  2024cites this paper
• A systematic approach of vitrification by high pressure freezing

  2024cites this paper
• Red light mediates the exocytosis of vasodilatory vesicles from cultured endothelial cells: a cellular, and ex vivo murine model

  2024cites this paper
• Electron Microscopy Techniques for 3D Plant ER Imaging.

  2024cites this paper
• Electron tomography unravels new insights into fiber cell wall nanostructure; exploring 3D macromolecular biopolymeric nano-architecture of spruce fiber secondary walls

  2023cites this paper
• LDO proteins and Vac8 form a vacuole-lipid droplet contact site required for lipophagy in response to starvation

  2023cites this paper
• Genetically controlled mtDNA deletions prevent ROS damage by arresting oxidative phosphorylation

  2022cites this paper
• Using reporters of different misfolded proteins reveals differential strategies in processing protein aggregates

  2022cites this paper
• One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM

  2022cites this paper
• Large organellar changes occur during mild heat shock in yeast.

  2021cites this paper
• Microbe-Mediated Mn Oxidation—A Proposed Model of Mineral Formation

  2021cites this paper
• Sphingosine 1-phosphate mediates adiponectin receptor signaling essential for lipid homeostasis and embryogenesis

  2021cites this paper
• Large organellar changes occur during mild heat shock in yeast

  2021cites this paper
• Nuclear envelope budding is a response to cellular stress

  2021cites this paper
• Unpicking the Secrets of African Swine Fever Viral Replication Sites

  2021cites this paper
• Snd3 controls nucleus-vacuole junctions in response to glucose signaling.

  2021cites this paper
• Analysis of Viruses in the Cellular Context by Electron Tomography

  2021cites this paper
• Coronaviruses: Methods and Protocols

  2020cites this paper
• High subzero cryofixation: A technique for observing ice within tissues.

  2020cites this paper
• Preparation of Cultured Cells Using High-Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope

  2020cites this paper
• Control of mitochondrial superoxide production includes programmed mtDNA deletion and restoration

  2020cites this paper
• Supplementary Figure S1

  2019cites this paper
• The Importance of Sample Processing for Correlative Imaging (or, Rubbish In, Rubbish Out)

  2019cites this paper
• Mitochondrial protein enriched extracellular vesicles discovered in human melanoma tissues can be detected in patient plasma

  2019cites this paper
• Indirect visualization of endogenous nuclear actin by correlative light and electron microscopy (CLEM) using an actin-directed chromobody

  2019cites this paper
• CryoAPEX – an electron tomography tool for subcellular localization of membrane proteins

  2019cites this paper
• Mitophagy-Related Cell Death Mediated by Vacquinol-1 and TRPM7 Blockade in Glioblastoma IV

  2019cites this paper
• Nuclear envelope budding is an evolutionary conserved phenomenon

  2018cites this paper
• Ommochromes in invertebrates: biochemistry and cell biology

  2018cites this paper
• Electron Microscopic Analysis of the Plasma Membrane and Cell Surface Molecules

  2018cites this paper
• Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution

  2018cites this paper
• Microtome-integrated microscope system for high sensitivity tracking of in-resin fluorescence in blocks and ultrathin sections for correlative microscopy

  2017cites this paper
• Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells.

  2017cites this paper
• Extracellular vesicles in motion

  2017cites this paper
• Analyzing Lysosome-Related Organelles by Electron Microscopy.

  2017cites this paper
• A subgroup of mitochondrial extracellular vesicles discovered in human melanoma tissues are detectable in patient blood

  2017cites this paper
• Immunolocalization of Proteins in Fission Yeast by Electron Microscopy.

  2017influential citation
• Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

  2017cites this paper
• Preserving the photoswitching ability of standard fluorescent proteins for correlative in-resin super-resolution and electron microscopy.

  2017cites this paper
• PROBING SECRETORY VESICLES AND LIPOSOME MODEL SYSTEMS USING NANOSCALE ELECTROCHEMISTRY AND MASS SPECTROMETRY

  2016cites this paper
• Exosomes purified from a single cell type have diverse morphology

  2016cites this paper
• Cryo-electron Microscopy Structure of the Native Prototype Foamy Virus Glycoprotein and Virus Architecture

  2016cites this paper
• Methods for monitoring lysosomal morphology.

  2015cites this paper
• Quantitative analysis of cytoskeletal reorganization during epithelial tissue sealing by large-volume electron tomography

  2015cites this paper
• Prion aggregate structure in yeast cells is determined by the Hsp104-Hsp110 disaggregase machinery

  2015cites this paper
• Herpes Simplex Virus 1 Us3 Deletion Mutant is Infective Despite Impaired Capsid Translocation to the Cytoplasm

  2015cites this paper
• Correlative in-resin super-resolution and electron microscopy using standard fluorescent proteins

  2015cites this paper
• A Cholesterol‐Dependent Endocytic Mechanism Generates Midbody Tubules During Cytokinesis

  2015cites this paper
• Coronaviruses

  2015cites this paper
• Step by step manipulation of the CryoCapsule with HPM high pressure freezers.

  2014cites this paper
• High Pressure Freezing/Freeze Substitution Fixation Improves the Ultrastructural Assessment of Wolbachia Endosymbiont – Filarial Nematode Host Interaction

  2014cites this paper
• Resolution of the three dimensional structure of components of the glomerular filtration barrier

  2014cites this paper
• Comparative ultrastructural characterization of African horse sickness virus-infected mammalian and insect cells reveals a novel potential virus release mechanism from insect cells.

  2014cites this paper
• Pathogen-host reorganisation during Chlamydia invasion revealed by cryo-electron tomography 1

  2014influential citation
• The complex ultrastructure of the endolysosomal system.

  2014cites this paper
• Correlative light and electron microscopy for a free-floating spindle in Xenopus laevis egg extracts.

  2014cites this paper
• Generation of Unique Poliovirus RNA Replication Organelles

  2014cites this paper
• Closer to the native state. Critical evaluation of cryo-techniques for Transmission Electron Microscopy: preparation of biological samples.

  2014influential citation
• Correlative light- and electron microscopy with chemical tags.

  2014cites this paper
• Three-dimensional imaging of adherent cells using FIB/SEM and STEM.

  2014cites this paper
• The CryoCapsule: Simplifying Correlative Light to Electron Microscopy

  2014cites this paper
• Preparation of Cultured Cells Using High-Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope

  2014cites this paper
• Pathogen–host reorganization during Chlamydia invasion revealed by cryo-electron tomography

  2014influential citation
• Infectious Bronchitis Virus Generates Spherules from Zippered Endoplasmic Reticulum Membranes

  2013cites this paper
• High-pressure freezing for scanning transmission electron tomography analysis of cellular organelles.

  2013cites this paper
• Endoplasmic reticulum–endosome contact increases as endosomes traffic and mature

  2013cites this paper
• Out with the old and in with the new: rapid specimen preparation procedures for electron microscopy of sectioned biological material

  2013cites this paper
• Rapid Embedding Methods into Epoxy and LR White Resins for Morphological and Immunological Analysis of Cryofixed Biological Specimens

  2013cites this paper
• Immunohistochemical localisation of caffeine in young tea ( Camellia sinensis (L.) O. Kuntze) leaves

  2012cites this paper
• Cygnet River Virus, a Novel Orthomyxovirus from Ducks, Australia

  2012cites this paper
• Bridging microscopes: 3D correlative light and scanning electron microscopy of complex biological structures.

  2012cites this paper
• An improved procedure for low‐temperature embedding of high‐pressure frozen and freeze‐substituted plant tissues resulting in excellent structural preservation and contrast

  2012cites this paper
• Chlamydiae Assemble a Pathogen Synapse to Hijack the Host Endoplasmic Reticulum

  2012cites this paper
• Heritable yeast prions have a highly organized three-dimensional architecture with interfiber structures

  2012cites this paper
• Quantitative evaluation of freeze-substitution effects on preservation of nuclear antigens during preparation of biological samples for immunoelectron microscopy

  2012influential citation
• Mapping the Distribution of the EPS Matrix Within Mixed Microbial Flocs

  2012cites this paper
• Preparation of plant cells for transmission electron microscopy to optimize immunogold labeling of carbohydrate and protein epitopes

  2012cites this paper
• The origins and evolution of freeze-etch electron microscopy.

  2011cites this paper
• Transcriptional and cellular responses to defective mitochondrial proteolysis in fission yeast.

  2011cites this paper
• Cyclin-Dependent Kinase 16/PCTAIRE Kinase 1 Is Activated by Cyclin Y and Is Essential for Spermatogenesis

  2011cites this paper
• Structural Analysis of Macromolecular Assemblies by Electron Microscopy

  2011cites this paper
• Freeze substitution in 3 hours or less

  2011cites this paper
• Intracellular membrane traffic at high resolution.

  2010cites this paper
• Saccharomyces cerevisiae nuclear and nucleolar antigen preservation for immunoelectron microscopy.

  2010cites this paper
• "Tips and tricks" for high-pressure freezing of model systems.

  2010cites this paper
• Preparation techniques for transmission electron microscopy of Hydra.

  2010cites this paper
• Ultrastructural investigation methods for Trypanosoma brucei.

  2010cites this paper
• High-pressure freezing combined with in vivo-DAB-cytochemistry: a novel approach for studies of endocytic compartments.

  2010cites this paper
• Strategies to improve the antigenicity, ultrastructure preservation and visibility of trafficking compartments in Arabidopsis tissue.

  2010cites this paper
• Toward sub-second correlative light and electron microscopy of Saccharomyces cerevisiae.

  2010cites this paper
• Comparison of methods of high-pressure freezing and automated freeze-substitution of suspension cells combined with LR White embedding

  2010cites this paper
• Three-dimensional structure of Rubella virus factories

  2010cites this paper
• Electron microscopy of flatworms standard and cryo-preparation methods.

  2010cites this paper
• Preparation of cryofixed cells for improved 3D ultrastructure with scanning transmission electron tomography

  2010influential citation
• A Single Method for Cryofixation and Correlative Light, Electron Microscopy and Tomography of Zebrafish Embryos

  2009influential citation
• Self‐pressurized rapid freezing (SPRF): a novel cryofixation method for specimen preparation in electron microscopy

  2009cites this paper