Methotrexate inhibits integrin adhesion molecules in the mouse model of pleurisy induced by carrageenan

The aim of this work was to analyze the effect of methotrexate (MTX) upon leukocyte migration and expression of adhesion molecules CD11a/CD18 in the lung, 4 and 48 h after inflammation induction by carrageenan in mice. The results showed that MTX significantly decreased leukocyte influx and CD11a expression in the lung at 4 and 48 h of pleurisy (P < 0.01). MTX also inhibited CD18 expression at 4 h but not 48 h of pleurisy (P < 0.01). These results proved that MTX at the studied doses had important anti-inflammatory properties, acting primarily on leukocyte migration from the pleural cavity to the lung via inhibition of CD11a/CD18 expression in the mouse model of inflammation.

• Publication year

  2007

• Venue

  Brazilian Archives of Biology and Technology

• Publication date

  2007-09-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1590/S1516-89132007000500005
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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  2004cited by this paper
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  2004cited by this paper
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  2004cited by this paper
• [Inhibition of joint destruction by methotrexate].

  2003cited by this paper
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  2003cited by this paper
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  2002cited by this paper
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  2002cited by this paper
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  2002cited by this paper
• Effects of methotrexate on nucleotide pools in normal human T cells and the CEM T cell line.

  2000cited by this paper
• Circulating levels of soluble E‐selectin, ICAM‐1 and VCAM‐1 in bullous pemphigoid during low‐dose methotrexate therapy

  2000cited by this paper
• Mechanisms of action of methotrexate.

  2000influential reference
• Inhibition of neutrophil migration soon after initiation of treatment with leflunomide or methotrexate in patients with rheumatoid arthritis: findings in a prospective, randomized, double-blind clinical trial in fifteen patients.

  2000cited by this paper
• The effect of suramin on bleomycin-induced lung injury.

  2000cited by this paper
• Reversal of the antiinflammatory effects of methotrexate by the nonselective adenosine receptor antagonists theophylline and caffeine: evidence that the antiinflammatory effects of methotrexate are mediated via multiple adenosine receptors in rat adjuvant arthritis.

  2000cited by this paper
• Methotrexate suppresses the interleukin-6 induced generation of reactive oxygen species in the synoviocytes of rheumatoid arthritis.

  2000cited by this paper
• Enhanced production of tissue inhibitor of metalloproteinases by peripheral blood mononuclear cells of rheumatoid arthritis patients responding to methotrexate treatment.

  2000cited by this paper
• Molecular and cellular effects of methotrexate.

  1999cited by this paper
• Methotrexate inhibits the first committed step of purine biosynthesis in mitogen-stimulated human T-lymphocytes: a metabolic basis for efficacy in rheumatoid arthritis?

  1999cited by this paper
• Effects of cyclosporine A and methotrexate on CD18 expression in recipients of rat cardiac allografts.

  1998cited by this paper
• Longterm prospective study of methotrexate in rheumatoid arthritis: conclusion after 132 months of therapy.

  1998cited by this paper
• Methotrexate and sulfasalazine promote adenosine release by a mechanism that requires ecto-5'-nucleotidase-mediated conversion of adenine nucleotides.

  1998cited by this paper
• Antiinflammatory and immunoregulatory action of methotrexate in the treatment of rheumatoid arthritis: evidence of increased interleukin-4 and interleukin-10 gene expression demonstrated in vitro by competitive reverse transcriptase-polymerase chain reaction.

  1998cited by this paper
• FK506 in combination with methotrexate for the prevention of graft-versus-host disease after marrow transplantation from matched unrelated donors.

  1996cited by this paper
• Changes in T‐cell phenotype and adhesion molecules expression in psoriatic lesions after low‐dose cyclosporin therapy

  1996cited by this paper
• Anti‐inflammatory effects of theophylline, cromolyn and salbutamol in a murine model of pleurisy

  1996influential reference
• Suppression of collagen-induced arthritis by an angiogenesis inhibitor, AGM-1470, in combination with cyclosporin: reduction of vascular endothelial growth factor (VEGF).

  1995cited by this paper
• Cyclosporine, methotrexate, and prednisone compared with cyclosporine and prednisone for prophylaxis of acute graft-versus-host disease.

  1993cited by this paper
• Acute graft-versus-host disease: analysis of risk factors after allogeneic marrow transplantation and prophylaxis with cyclosporine and methotrexate.

  1992cited by this paper
• Methotrexate as an adjunct in the treatment of persistent mild cardiac allograft rejection.

  1990cited by this paper
• Graft-versus-host disease as adoptive immunotherapy in patients with advanced hematologic neoplasms.

  1989cited by this paper
• Methotrexate and cyclosporine compared with cyclosporine alone for prophylaxis of acute graft versus host disease after marrow transplantation for leukemia.

  1986cited by this paper
• Mechanisms involved in acute lung edema induced in dogs by oleic acid.

  1986cited by this paper
• Recent concepts on the mechanism of action of methotrexate.

  1981cited by this paper
• Recent concepts on the mechanism of action of methotrexate.

  1981cited by this paper
• Effects of cytotoxic immunosuppressants on tuberculin-sensitive lymphocytes in guinea pigs.

  1975cited by this paper
• Advances in chemotherapy of cancer in man.

  1956cited by this paper

• Polygala molluginifolia A. St.-Hil. and Moq. prevent inflammation in the mouse pleurisy model by inhibiting NF-κB activation.

  2014cites this paper
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  2010cites this paper