Role of MAP kinase cascades in inducing arginine transporters and nitric oxide synthetase in RAW264 macrophages

Bacterial lipopolysaccharide (LPS) in the presence of interferon gamma (IFNγ) stimulates the synthesis of the cationic amino acid transporter 2B (CAT‐2B) and inducible nitric oxide synthetase (iNOS) in RAW264 macrophages, which are thought to underlie the increased rate of arginine uptake into these cells and its conversion to nitric oxide, respectively. Here I demonstrate that the LPS‐ and IFNγ‐induced increase in arginine uptake into RAW264 cells is partially suppressed in the presence of PD 98059, partially suppressed in the presence of SB 203580, and completely inhibited by both drugs. In contrast, the LPS‐ and IFNγ‐induced synthesis of CAT‐2B mRNA and iNOS protein is unaffected by PD 98059 and SB 203580. The results indicate that the MAPK/ERK and SAPK2/p38 cascades are both rate‐limiting for LPS‐ and IFNγ‐stimulated arginine uptake, but not for iNOS synthesis. They also suggest that PD 98059 and SB 203580 suppress CAT‐2B synthesis at a post‐transcriptional level.

• Publication year

  1998

• Venue

  FEBS Letters

• Publication date

  1998-06-16

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S0014-5793(98)00578-XPMID 9662426
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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