A nickel chelate microtiter plate assay for six histidine-containing proteins.

No abstract is available for this paper.

• Publication year

  1996

• Venue

  Analytical Biochemistry

• Publication date

  1996-02-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1006/ABIO.1996.0050PMID 8742083
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Detection and quantitation of hexa-histidine-tagged recombinant proteins on western blots and by a surface plasmon resonance biosensor technique.

  1995cited by this paper
• Identification of the factor VIIa binding site on tissue factor by homologous loop swap and alanine scanning mutagenesis.

  1994cited by this paper
• Recursive PCR: a novel technique for total gene synthesis.

  1992cited by this paper
• Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.

  1991cited by this paper
• Secretion in yeast. Purification and in vitro translocation of chemical amounts of prepro-alpha-factor.

  1991cited by this paper
• Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.

  1991cited by this paper
• Expression and purification of the leucine zipper and DNA-binding domains of Fos and Jun: both Fos and Jun contact DNA directly.

  1990cited by this paper
• Rapid purification of homodimer and heterodimer HIV-1 reverse transcriptase by metal chelate affinity chromatography.

  1990cited by this paper
• Bioassay for trans-activation using purified human immunodeficiency virus tat-encoded protein: trans-activation requires mRNA synthesis.

  1989cited by this paper
• Human tissue factor: cDNA sequence and chromosome localization of the gene.

  1987cited by this paper
• New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.

  1987cited by this paper
• Metal chelate affinity chromatography, a new approach to protein fractionation

  1975cited by this paper

• Immobilization of aldoxime dehydratases on metal affinity resins and use of the immobilized catalysts for the synthesis of nitriles important in fragrance industry.

  2024cites this paper
• Convenient site-selective protein coupling from bacterial raw lysates to coenzyme A-modified tobacco mosaic virus (TMV) by Bacillus subtilis Sfp phosphopantetheinyl transferase.

  2023cites this paper
• Co-Assembly of Liposomes, Dendrimersomes, and Polymersomes with Amphiphilic Janus Dendrimers Conjugated to Mono- and Tris-Nitrilotriacetic Acid (NTA, TrisNTA) Enhances Protein Recruitment

  2021cites this paper
• Advancements of Mass Spectrometry in Biomedical Research

  2019influential citation
• Encapsulation of hydrophobic components in dendrimersomes and decoration of their surface with proteins and nucleic acids

  2019cites this paper
• Selective Derivatization of Hexahistidine-Tagged Recombinant Proteins.

  2019cites this paper
• Development of a high-throughput assay to detect antibody inhibition of low pH induced conformational changes of influenza virus hemagglutinin

  2018cites this paper
• A Flexible Method for the Stable, Covalent Immobilization of Enzymes at Electrode Surfaces

  2017cites this paper
• Controlled immobilization of His-tagged proteins for protein-ligand interaction experiments using Ni2+-NTA layer on glass surfaces

  2016cites this paper
• Controlled immobilization of His-tagged proteins for protein-ligand interaction

  2015cites this paper
• Eukaryotic Expression System Pichia pastoris Affects the Lipase Catalytic Properties: A Monolayer Study

  2014cites this paper
• Equilibrium adsorption of hexahistidine on pH-responsive hydrogel nanofilms.

  2014cites this paper
• New assay for quantification of PEGylated proteins during in vitro permeability studies.

  2014cites this paper
• DNA-binding site recognition by bHLH and mads-domain transcription factors

  2013cites this paper
• Think modular: a simple apoferritin-based platform for the multifaceted detection of pancreatic cancer.

  2013cites this paper
• Molecular beacon aptamers for direct and universal quantitation of recombinant proteins from cell lysates.

  2012cites this paper
• Continuous administration of recombinant human endostatin (Endostar): A pre-clinical safety study.

  2012cites this paper
• Microplate assay for aptamer-based thrombin detection using a DNA-enzyme conjugate based on histidine-tag chemistry.

  2012cites this paper
• A drug discovery platform: a simplified immunoassay for analyzing HIV protease activity.

  2012cites this paper
• Encapsulation of Nanoparticles Using Nitrilotriacetic Acid End‐Functionalized Polystyrenes and Their Application for the Separation of Proteins

  2012cites this paper
• Expression of recombinant human interleukin-8 and its purification using a single buffer system.

  2011cites this paper
• Recombinant antigen microarrays for serum/plasma antibody detection.

  2011cites this paper
• DNA-enzyme conjugate with a weak inhibitor that can specifically detect thrombin in a homogeneous medium.

  2011cites this paper
• Peptide-protein microarrays and surface plasmon resonance detection: biosensors for versatile biomolecular interaction analysis.

  2010influential citation
• Protein immobilization on Ni(II) ion patterns prepared by microcontact printing and dip-pen nanolithography.

  2010cites this paper
• One-step purification and immobilization of His-tagged rhamnosidase for naringin hydrolysis.

  2010cites this paper
• Miniaturized parallel screens to identify chromatographic steps required for recombinant protein purification

  2010cites this paper
• Hexahistidine-tag-specific optical probes for analyses of proteins and their interactions.

  2010cites this paper
• Specific and reversible immobilization of histidine-tagged proteins on functionalized silicon nanowires

  2010cites this paper
• Regeneration of IMAC-Cu Proteinchip by Borate Eluent

  2010cites this paper
• Use of intein-mediated protein ligation strategies for the fabrication of functional protein arrays.

  2009cites this paper
• Plate capture assay of fluorescent oligonucleotide duplex reporter-transcription factor complexes.

  2009cites this paper
• Reusable linking chemistry for His‐6 tagged proteins in an affinity‐based porous silicon biosensor

  2009cites this paper
• Interactions between EB1 and Microtubules

  2009cites this paper
• Protein immobilization without purification via dip-pen nanolithography.

  2008cites this paper
• Chitosan fibers: versatile platform for nickel-mediated protein assembly.

  2008cites this paper
• Recent Advances in Controlled Immobilization of Proteins onto the Surface of the Solid Substrate and Its Possible Application to Proteomics

  2008cites this paper
• Arg-Gly-Asp-containing domains of fibrillins-1 and -2 distinctly regulate lung fibroblast migration.

  2008cites this paper
• Ultrasensitive optical biodiagnostic methods using metallic nanoparticles.

  2008cites this paper
• Chicken single-chain antibody fragments directed against recombinant VP7 of bluetongue virus

  2008cites this paper
• Simplified approaches for the development of an ELISA to detect circulating autoantibodies to p53 in cancer patients

  2008cites this paper
• Study of the sensitization of tetradecyl benzyl dimethyl ammonium chloride for the color development reaction between lysine and sodium 1,2-naphthoquinone-4-sulfonate and the determination of lysine in pharmaceutical and biological samples.

  2007cites this paper
• General method for site-specific protein immobilization by Staudinger ligation.

  2007cites this paper
• Two methods for glass surface modification and their application in protein immobilization.

  2007cites this paper
• One-step purification and immobilization of his-tagged GL-7-ACA acylase

  2007cites this paper
• Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry

  2007cites this paper
• Oriented immobilization of epidermal growth factor onto culture substrates for the selective expansion of neural stem cells.

  2007cites this paper
• Exploring enzymatic catalysis at a solid surface: a case study with transglutaminase-mediated protein immobilization.

  2007cites this paper
• Enzyme-linked immunosorbent assays for insulin-like growth factor-I using six-histidine tag fused proteins.

  2007cites this paper
• Affinity Immobilization of Tagged Enzymes

  2006cites this paper
• Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays

  2006cites this paper
• Reversible coordinative bonds in molecular recognition.

  2006cites this paper
• High‐throughput process development for recombinant protein purification

  2006cites this paper
• Generation of High Density Protein Microarrays by Cell-free in Situ Expression of Unpurified PCR Products *

  2006cites this paper
• Expression and characterization of a housefly cecropin gene in the methylotrophic yeast, Pichia pastoris.

  2006cites this paper
• Application of an enzyme chip to the microquantification of l-phenylalanine.

  2006cites this paper
• Molecular Imaging with Quantum Dots Probing EMT and Prostate Cancer Metastasis in Live Animals

  2006cites this paper
• The effect of the hexahistidine-tag in the oligomerization of HSC70 constructs.

  2006cites this paper
• Physical controls on directed virus assembly at nanoscale chemical templates.

  2006cites this paper
• Surface modifications for enhanced immobilization of biomolecules: applications in biocatalysts and immuno-biosensor

  2006influential citation
• Artificial polypeptide scaffold for protein immobilization.

  2005cites this paper
• Intein-mediated, in vitro and in vivo protein modifications with small molecules.

  2005cites this paper
• Do metals inhibit acetylcholinesterase (AChE)? Implementation of assay conditions for the use of AChE activity as a biomarker of metal toxicity

  2005cites this paper
• Site-Specific Immobilization of Proteins in a Microarray

  2005cites this paper
• Application of microarrays in high-throughput enzymatic profiling

  2004cites this paper
• A polymer scaffold for protein oligomerization.

  2004cites this paper
• Strategies for immobilization of biomolecules in a microarray.

  2004cites this paper
• Site-specific immobilization of biotinylated proteins for protein microarray analysis.

  2004cites this paper
• A Metal‐Chelating Piezoelectric Sensor Chip for Direct Detection and Oriented Immobilization of PolyHis‐Tagged Proteins

  2004cites this paper
• Affinity Methods to Immobilize Acetylcholinesterases for Manufacturing Biosensors

  2004cites this paper
• Development of Highly Sensitive Sensor Based on Bioengineered Acetylcholinesterase Immobilized by Affinity Method

  2003cites this paper
• Array-based technologies and their applications in proteomics.

  2003cites this paper
• Supramolecular Self-Assembly of Lipid Derivatives on Carbon Nanotubes

  2003cites this paper
• Selection of novel structural zinc sites from a random peptide library

  2003cites this paper
• Biocombinatorial selection of metal ion-chelating peptides

  2003cites this paper
• Solid supports for microarray immunoassays

  2003cites this paper
• Intein-mediated biotinylation of proteins and its application in a protein microarray.

  2002cites this paper
• Antibody microarrays: promises and problems.

  2002cites this paper
• SwellGel: an affinity chromatography technology for high-capacity and high-throughput purification of recombinant-tagged proteins.

  2001cites this paper
• IMMOBILIZATION OF ENZYMES ON SCREEN-PRINTED SENSORS VIA AN HISTIDINE TAIL. APPLICATION TO THE DETECTION OF PESTICIDES USING MODIFIED CHOLINESTERASE

  2001cites this paper
• Relative position of the hexahistidine tag effects binding properties of a tumor-associated single-chain Fv construct.

  2000cites this paper
• Chelated mercury as a ligand in immobilized metal ion affinity chromatography of proteins.

  2000cites this paper
• Ni-NTA-gold clusters target His-tagged proteins.

  1999cites this paper
• Methods of screening combinatorial libraries using immobilized or restrained receptors.

  1999cites this paper
• Nickel-induced oligomerization of proteins containing 10-histidine tags.

  1998cites this paper
• The development of new biotechnologies using metalloprotein design.

  1998cites this paper
• The use of Ni-nitrilotriacetic acid agarose for estimation of affinities of hexahistidine-tagged Fab to single-stranded DNA.

  1997cites this paper
• High-throughput screening: advances in assay technologies.

  1997cites this paper