Use of intein-mediated protein ligation strategies for the fabrication of functional protein arrays.

No abstract is available for this paper.

• Publication year

  2009

• Venue

  Methods in Enzymology

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0076-6879(09)62010-3PMID 19632476
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Comparative properties of insulin-like growth factor 1 (IGF-1) and [Gly7D-Ala]IGF-1 prepared by total chemical synthesis.

  2008cited by this paper
• Linking chemistry and biology for the study of protein function.

  2007cited by this paper
• Applications of protein microarray technology.

  2007cited by this paper
• Post-translational enzyme activation in an animal via optimized conditional protein splicing.

  2007cited by this paper
• Development of efficient methods for accomplishing cysteine-free peptide and glycopeptide coupling.

  2007cited by this paper
• Building complex glycopeptides: Development of a cysteine-free native chemical ligation protocol.

  2006cited by this paper
• Protein and small molecule microarrays: powerful tools for high-throughput proteomics.

  2006cited by this paper
• Protein chip fabrication by capture of nascent polypeptides

  2006cited by this paper
• C‐Terminal Modifications of a Protein by UAG‐Encoded Incorporation of Puromycin during in vitro Protein Synthesis in the Absence of Release Factor 1

  2006cited by this paper
• Evolving strategies for protein synthesis converge on native chemical ligation.

  2006cited by this paper
• Protein ligation: an enabling technology for the biophysical analysis of proteins

  2006cited by this paper
• Selective immobilization of proteins onto solid supports through split-intein-mediated protein trans-splicing.

  2006cited by this paper
• Site-specific protein modification through Cu(I)-catalyzed 1,2,3-triazole formation and its implementation in protein microarray fabrication.

  2006cited by this paper
• Large-scale identification of protein-protein interaction of Escherichia coli K-12.

  2006cited by this paper
• Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays

  2006cited by this paper
• Protein microarray technology

  2006cited by this paper
• Kinetically controlled ligation for the convergent chemical synthesis of proteins.

  2006cited by this paper
• Site-selective protein immobilization by Staudinger ligation.

  2006cited by this paper
• Artificial polypeptide scaffold for protein immobilization.

  2005cited by this paper
• Protein microarrays as tools for functional proteomics.

  2005cited by this paper
• Towards a proteome-scale map of the human protein–protein interaction network

  2005cited by this paper
• Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.

  2005cited by this paper
• A ligation and photorelease strategy for the temporal and spatial control of protein function in living cells.

  2005cited by this paper
• Diels-Alder ligation and surface immobilization of proteins.

  2005cited by this paper
• Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing.

  2005cited by this paper
• Proteomics and genomics

  2005cited by this paper
• Labeling proteins with small molecules by site-specific posttranslational modification.

  2004cited by this paper
• Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo.

  2004cited by this paper
• A one-pot total synthesis of crambin.

  2004cited by this paper
• Photocontrol of Smad2, a multiphosphorylated cell-signaling protein, through caging of activating phosphoserines.

  2004cited by this paper
• Chemoselective attachment of biologically active proteins to surfaces by expressed protein ligation and its application for "protein chip" fabrication.

  2004cited by this paper
• Enzyme assays for high-throughput screening.

  2004cited by this paper
• Domain-specific incorporation of noninvasive optical probes into recombinant proteins.

  2004cited by this paper
• Expanding the scope of site-specific protein biotinylation strategies using small molecules.

  2004cited by this paper
• Versatile protein biotinylation strategies for potential high-throughput proteomics.

  2004cited by this paper
• Regulation of Gene Expression by a Metabolic Enzyme

  2004cited by this paper
• Self-Assembling Protein Microarrays

  2004cited by this paper
• Covalent and selective immobilization of fusion proteins.

  2003cited by this paper
• Synthesis of protein-nucleic acid conjugates by expressed protein ligation.

  2003cited by this paper
• Semisynthesis of proteins by expressed protein ligation.

  2003cited by this paper
• Cell-permeable small molecule probes for site-specific labeling of proteins.

  2003cited by this paper
• Site-specific protein immobilization by Staudinger ligation.

  2003cited by this paper
• Protein microarray technology.

  2002cited by this paper
• Intein-mediated biotinylation of proteins and its application in a protein microarray.

  2002cited by this paper
• New methods for proteomic research: preparation of proteins with N-terminal cysteines for labeling and conjugation.

  2002cited by this paper
• Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands

  2002cited by this paper
• A quantitative assessment of heterogeneity for surface-immobilized proteins.

  2001cited by this paper
• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• Global Analysis of Protein Activities Using Proteome Chips

  2001cited by this paper
• Intein-Mediated Synthesis of Proteins Containing Carbohydrates and Other Molecular Probes

  2000cited by this paper
• Fusions to self-splicing inteins for protein purification.

  2000cited by this paper
• Biotinylation of proteins in vivo: a useful posttranslational modification for protein analysis.

  2000cited by this paper
• Controlled intracellular processing of fusion proteins by TEV protease.

  2000cited by this paper
• Specific bonding of puromycin to full-length protein at the C-terminus.

  2000cited by this paper
• Analysis of yeast protein kinases using protein chips

  2000cited by this paper
• Printing proteins as microarrays for high-throughput function determination.

  2000cited by this paper
• Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step.

  1998cited by this paper
• Manual manufacturing of oligonucleotide, DNA, and protein microchips.

  1997cited by this paper
• A nickel chelate microtiter plate assay for six histidine-containing proteins.

  1996cited by this paper
• Synthesis of proteins by native chemical ligation.

  1994cited by this paper
• Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins.

  1990cited by this paper

• G Protein-Coupled Receptors: Immobilization and Applications in Drug Discovery

  2023cites this paper
• Protein engineering to capture and kill cancer cells

  2015cites this paper
• Chemical synthesis of proteins using N-sulfanylethylanilide peptides, based on N-S acyl transfer chemistry.

  2015cites this paper
• Semi-Synthesis of Labeled Proteins for Spectroscopic Applications

  2013cites this paper
• Diverse organo-peptide macrocycles via a fast and catalyst-free oxime/intein-mediated dual ligation.

  2012cites this paper
• Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance

  2012cites this paper
• Biologically-Inspired Responsive Materials: Integrating Biological Function into Synthetic Materials

  2012cites this paper
• Selective and direct immobilization of cysteinyl biomolecules by electrochemical cleavage of azo linkage.

  2010cites this paper