CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE

Measurements of actinomycin-3H binding in synchronized HeLa cells reveal that the binding capacity of chromatin decreases progressively during the S phase despite a doubling of nuclear DNA content, reaches a minimal level during G2 and mitosis, and then increases gradually throughout the subsequent G1 interval. Since this pattern was evident in experiments with living cells, ethanol-fixed cells, and isolated nuclei, but not with purified DNA, the actinomycin binding profile may reflect changes in the degree of association between DNA and chromosomal proteins at different stages of the cell cycle.

• Publication year

  1972

• Venue

  Journal of Cell Biology

• Publication date

  1972-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.55.2.322PMID 5076779PMCID 2108799
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Chromatin structure and the cell cycle.

  1972cited by this paper
• EFFECTS OF ACTINOMYCIN D AND PUROMYCIN ON THE CELL PROGRESS FROM M TO G1 AND S STAGES IN CULTURED MOUSE LEUKEMIA L5178Y CELLS

  1972cited by this paper
• A METHOD FOR IMPROVING SYNCHRONY IN THE G2 PHASE OF THE CELL CYCLE

  1971cited by this paper
• ON THE DIFFERENTIAL CYTOTOXICITY OF ACTINOMYCIN D

  1971cited by this paper
• Actinomycin D binding to isolated deoxyribonucleoprotein and intact cells.

  1970cited by this paper
• Chromatin and Histones: Binding of Tritiated Actinomycin D to Heterochromatin in Mealy Bugs

  1969cited by this paper
• Binding of Tritiated Actinomycin and Cell Differentiation

  1969cited by this paper
• Actinomycin binding of normal and phytohaemagglutinin stimulated lymphocytes.

  1969cited by this paper
• Changes in deoxyribonucleo-protein during spermiogenesis in the bull. 3 H-Actinomycin D binding capacity.

  1969cited by this paper
• Cyclic x-ray responses in mammalian cells in vitro.

  1968cited by this paper
• Chromosome aberrations and the cell cycle.

  1968cited by this paper
• Electron microscopic studies of detergent-treated HeLa cell nuclei.

  1966cited by this paper
• X-ray sensitivity during the cell generation cycle of cultured Chinese hamster cells.

  1966cited by this paper
• ACTINOMYCIN BINDING TO DNA: MECHANISM AND SPECIFICITY.

  1965cited by this paper
• Phasing, Mitotic Delay, and Chromosomal Aberrations in Mammalian Cells.

  1964cited by this paper
• COMMENTS ON THE CELL LIFE CYCLE.

  1964cited by this paper
• LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION.

  1963cited by this paper
• Action of radiation on mammalian cells. IV. Reversible mitotic lag in the S3 HeLa cell produced by low doses of x-rays.

  1961cited by this paper
• Amino acid metabolism in mammalian cell cultures.

  1959cited by this paper

• An Intermittent Cytochemist

  2023cites this paper
• Epigenetics and Beyond: Targeting Histone Methylation to Treat Type 2 Diabetes Mellitus

  2022cites this paper
• Cell cycle– and genomic distance–dependent dynamics of a discrete chromosomal region

  2019cites this paper
• CRISPR-Based DNA Imaging in Living Cells Reveals Cell Cycle-Dependent Chromosome Dynamics

  2017cites this paper
• Chick-Erythrocyte Nucleus Reactivation ii by Inhibitors of Proteolytic Enzymes

  2016cites this paper
• Chromatin condensation dynamics and implications of induced premature chromosome condensation.

  2013cites this paper
• UNSCHEDULED DNA SYNTHESIS INDUCED IN BARLEY SEEDS BY GAMMA RAYS AND 4-NITROQUINOLINE 1-OXIDEI)

  2007cites this paper
• The phenomenon of premature chromosome condensation: its relevance to basic and applied research

  2004cites this paper
• The spatial organization of the genome in mammalian cells.

  2004cites this paper
• Induction of DNA double-strand breaks at various stages of the cell cycle using the comet assay

  2002cites this paper
• Nuclear morphology during the S phase

  1998cites this paper
• DNA changes involved in the formation of metaphase chromosomes, as observed in mouse duodenal crypt cells stained by osmium‐ammine I. New structures arise during the S phase and condense at prophase into “chromomeres,” which fuse at prometaphase into mitotic chromosomes

  1995cites this paper
• [Structure of chromatin and cell cycle. 3].

  1995cites this paper
• Subdivision of the mitotic cycle into eleven stages, on the basis of the chromosomal changes observed in mouse duodenal crypt cells stained by the DNA‐specific feulgen reaction

  1994cites this paper
• Cytological and immunocytochemical approaches to the study of corneal endothelial wound repair.

  1994cites this paper
• Quality Control Issues in DNA Content Flow Cytometry

  1993cites this paper
• Detection of cell-cycle stage by fluorescence in situ hybridization: its application in human interphase cytogenetics.

  1992cites this paper
• Influence of pituitary grafts or prolactin administrations on the hormone sensitivity of ovarian hormone-independent mouse mammary MXT tumors.

  1989cites this paper
• An electron microscopic study of mitosis in mouse duodenal crypt cells confirms that the prophasic condensation of chromatin begins during the DNA-synthesizing (S) stage of the cycle.

  1989cites this paper
• Simple and complex cell cycles.

  1989cites this paper
• A model of chromatin-dependent DNA replication sequences based on the decondensation units hypothesis.

  1989cites this paper
• The bound water fraction and the HeLa cell cycle.

  1988cites this paper
• Visualization of chromosome assembly during the S and G2 stages of the cycle and chromosome disassembly during the G1 stage in semithin sections of mouse duodenal crypt cells and other cells.

  1988cites this paper
• The chromosome cycle and the centrosome cycle in the mitotic cycle.

  1987cites this paper
• 7 – Regulation of Mitosis by Nonhistone Protein Factors in Mammalian Cells

  1987cites this paper
• Relationship between nuclear DNA synthesis and centrosome reproduction in sea urchin eggs.

  1987cites this paper
• Influence of DNA-binding drugs on chromatin condensation.

  1986cites this paper
• Mutational extinction induced by sequential X irradiation and actinomycin D treatments in Chinese hamster ovary cells.

  1986cites this paper
• Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-amino-actinomycin D and single laser excitation: applications to study of cell activation and the cell cycle of murine Ly-1 B cells.

  1986cites this paper
• The technique of premature chromosome condensation to study the leukemic process: review and speculations.

  1986cites this paper
• Changes in DNA capacity for actinomycin-D binding in nuclei of interphase cells.

  1985cites this paper
• Cell cycle variations in chromatin structure detected by DNase I.

  1985cites this paper
• Histological staining by tritiated actinomycin D: a useful tool to study neoplastic cell heterogeneity and estrogen sensitivity of mammary tumors.

  1985cites this paper
• Inactivation of mitotic factors by ultraviolet irradiation of HeLa cells in mitosis.

  1984cites this paper
• Changes in density of chromatin in the meristematic cells ofSinapis alba during transition to flowering

  1984cites this paper
• The Ultrastructural Organization of Prematurely Condensed Chromosomes

  1984cites this paper
• Induction and repair of DNA strand breaks in CHO-cells irradiated in various phases of the cycle.

  1983cites this paper
• Evidence for the presence of inhibitors of mitotic factors during G1 period in mammalian cells

  1983cites this paper
• Polyoma-induced stimulation of nucleoplasmic transcription is paralleled by development of resistance against actinomycin D.

  1983cites this paper
• Studies on corneal endothelial growth and repair. IV. Changes in the surface during cell division as revealed by scanning electron microscopy.

  1983cites this paper
• Cell cycle-specific changes in the ultrastructural organization of prematurely condensed chromosomes

  1983cites this paper
• Replicative activity and actinomycin binding in mouse diploid fibroblasts (in vitro ageing).

  1983cites this paper
• 13 – Premature Chromosome Condensation In the Diagnosis of Malignancies

  1982cites this paper
• Nuclear 3,5,3'-triiodothyronine receptor concentration increases during deoxyribonucleic acid synthesis in partially synchronized GC cell cultures.

  1982cites this paper
• Cell cycle-dependent regulation of excision repair of UV damage.

  1982cites this paper
• Regulation of the cell cycle by somatomedins.

  1982cites this paper
• Relation of cell cycle position and chromatin decondensation to ectopic hormone synthesis in HeLa cells

  1982cites this paper
• 8 – Cell Cycle Analysis of Temperature-Sensitive Mutants by Premature Chromosome Condensation

  1982cites this paper
• 2 – Cell Cycle and Chromosome Cycle: Morphological and Functional Aspects

  1982cites this paper
• 1 – The Phenomenon of Premature Chromosome Condensation

  1982cites this paper
• 5 - Nuclear Fluorescence and Chromatin Condensation of Mammalian Cells during the Cell Cycle with Special Reference to the G 1 Phase

  1982cites this paper
• Premature Chromosome Condensation

  1982cites this paper
• Nuclear size and nuclear binding of tritiated actinomycin D into epithelial cells of colon cancer patients with apparently normal colorectal mucosa.

  1981cites this paper
• Fluorimetric measurements and chromatin condensation patterns of nuclei from 3T3 cells throughout G1

  1981cites this paper
• Length of human prematurely condensed chromosomes during G0 and G1 phase.

  1981cites this paper
• Regulation of Glycopeptide Hormone Synthesis in Cell Culture

  1981cites this paper
• Differential response of cycling and noncycling cells to inducers of DNA synthesis and mitosis

  1981cites this paper
• Observations on the Structure of Mechanically Stretched Chromatin Fibrils

  1981cites this paper
• Changes in non-histone proteins during mouse liver regeneration.

  1981cites this paper
• New cell cycle compartments identified by multiparameter flow cytometry.

  1980cites this paper
• Studies on corneal endothelial growth and repair. II. increased transcription as detected by incorporation of 3H-uridine and 3H-actinomycin D.

  1980cites this paper
• 8 – Water-Macromolecular Interactions during the Cell Cycle

  1980cites this paper
• Initiation of DNA synthesis in the prematurely condensed chromosomes of G1 cells

  1980cites this paper
• Role of nuclear proteins on [3H]actinomycin D binding during lymphocyte mitogenesis.

  1980cites this paper
• The quinternary chromatin-DNA structure

  1980cites this paper
• [Dynamics of incorporation of 3H-actinomycin D into synchronized Chinese hamster cells].

  1980cites this paper
• 3 – Events Associated with the Mitosis to G1 Transition in Mammalian Cells

  1980cites this paper
• Zellzyklus und Chromosomenzyklus

  1980cites this paper
• The quinternary chromatin-DNA structure. Three-dimensional reconstruction and functional significance.

  1980cites this paper
• Cell cycle dependent changes of chromosomes in mouse fibroblasts.

  1979cites this paper
• A probe into nuclear events during the cell cycle of Saccharomyces cerevisiae: studies of folded chromosomes in cdc mutants which arrest in G1

  1979cites this paper
• Role of spindle microtubules in the control of cell cycle timing

  1979cites this paper
• Cell differentiation in human gastric gland as revealed by nuclear binding of tritiated actinomycin.

  1979cites this paper
• Pattern of condensation of mouse and Chinese hamster chromosomes in G2 and mitosis of 33258-Hoechst-treated cells.

  1979cites this paper
• DNA damage in synchronized HeLa cells irradiated with ultraviolet.

  1979cites this paper
• Cell cycle analysis of sodium butyrate and hydroxyurea, inducers of ectopic hormone production in HeLa cells

  1979cites this paper
• Correlation between nuclear morphology and rate of deoxyribonucleic acid synthesis in a normal cell line.

  1979cites this paper
• Erythroid differentiation of Friend leukemia cells as studied by acridine orange staining and flow cytometry.

  1979cites this paper
• [Cell aging in vitro].

  1979cites this paper
• Mapping G1 phase by the structural morphology of the prematurely condensed chromosomes

  1978cites this paper
• Structure of interphase nuclei in relation to the cell cycle. Chromatin organization in mouse L cells temperature-sensitive for DNA replication

  1978cites this paper
• Cytochemical study of human lymphocytes stimulated by PHA in function of donor age.

  1978cites this paper
• STRUCTURAL CHANGES IN CHROMATIN DURING THE HELA CELL CYCLE: EFFECT ON WATER NMR RELAXATION TIMES

  1978cites this paper
• Supercoiling of DNA and nuclear conformation during the cell-cycle.

  1978cites this paper
• Nuclear binding of tritiated actinomycin in surface epithelial cells from normal stomach and atrophic gastritis.

  1978cites this paper
• Cell cycle-dependent changes in non-membranous nuclear ghosts from HeLa cells.

  1978cites this paper
• Inducers of DNA synthesis present during mitosis of mammalian cells lacking G1 and G2 phases.

  1978cites this paper
• Premature chromosome condensation and cell cycle analysis

  1977cites this paper
• DNA injuries, their repair, and carcinogenesis.

  1977cites this paper
• Interphase and metaphase chromatin. Different stainability of DNA with acridine orange after treatment at low pH.

  1977cites this paper
• 6 – Premature Chromosome Condensation and the Fine Structure of Chromosomes

  1977cites this paper
• Biochemical events associated with healing of a chemical injury in the rabbit lens.

  1977cites this paper
• DNA injuries, their repair, and carcinogenesis.

  1977cites this paper
• SEQUENTIAL BIOCHEMICAL EVENTS IN THE CELL CYCLE

  1977cites this paper
• Stem Cells, Nonproliferating Cells, and Their Kinetics in Normal and Neoplastic Tissues

  1977cites this paper
• Antibiotics: Nucleic Acids As Targets in Chemotherapy

  1977cites this paper
• 7 – CONTROL OF ANIMAL CELL PROLIFERATION

  1977cites this paper
• Objective identification of cell cycle phases and subphases by automated image analysis.

  1977cites this paper
• Effect of low doses of actinomycin D on RNA synthesis in chick embryo fibroblasts transformed by Rous sarcoma virus.

  1977cites this paper
• Different sensitivity of DNA in situ in interphase and metaphase chromatin to heat denaturation

  1977cites this paper