TimeLapse-seq: Adding a temporal dimension to RNA sequencing through nucleoside recoding

RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.

• Publication year

  2018

• Venue

  Nature Methods

• Publication date

  2018-01-22

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1038/nmeth.4582PMID 29355846PMCID 5831505
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Protocol for TimeLapse-seq

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