Macrophage migration from an agarose droplet: development of a micromethod for assay of delayed hypersensitivity.

Inhibition of macrophage migration from a glass capillary is the most widely accepted and best characterized in vitro correlate of delayed hypersensitivity. Significant technical limitations of this method have been eliminated by a modified migration inhibitory factor (MIF) method in which macrophages are stabilized in an agarose droplet instead of a capillary tube. This agarose method is technically simple, permitting the rapid assay of large numbers of samples and requiring relatively small volumes of culture supernatant. Experiments comparing the capillary and agarose methods have established the validity and specificity of the agarose method for assay of MIF.

• Publication year

  1973

• Venue

  Journal of Immunology

• Publication date

  1973-03-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.110.3.752PMID 4120226
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• An in vitro assay for cellular hypersensitivity in man.

  1970cited by this paper
• Communications. "In vitro" cell reactions in delayed hypersensitivity.

  1969cited by this paper
• Cell Migration Inhibition Factor released by Antigen from Human Peripheral Lymphocytes

  1968cited by this paper
• Mechanism of a Reaction in Vitro Associated with Delayed-Type Hypersensitivity

  1966cited by this paper
• DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS.

  1964cited by this paper
• IN VITRO STUDIES OF CELLULAR HYPERSENSITIVITY. I. SPECIFIC INHIBITION OF MIGRATION OF CELLS FROM ADJUVANT-IMMUNIZED ANIMALS BY PURIFIED PROTEIN DERIVATIVE AND OTHER PROTEIN ANTIGENS.

  1963cited by this paper
• In vitro Cell Migration as a Model for Delayed Hypersensitivity.∗

  1962cited by this paper

• Concentration-Dependent Pleiotropic Effects of Thymosin Beta4 and Cofilin on the Migratory Activity of Carcinoma Cells

  2025cites this paper
• Macrophage migration inhibitory factor facilitates astrocytic production of the CCL2 chemokine following spinal cord injury

  2022cites this paper
• In Vivo and in Vitro Cell-Mediated Responses in Coccidioidomycosis

  2015cites this paper
• MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells.

  2014cites this paper
• Collagen-like polypeptide poly(Pro-Hyp-Gly) conjugated with Gly-Arg-Gly-Asp-Ser and Pro-His-Ser-Arg-Asn peptides enchances cell adhesion, migration, and stratification.

  2011cites this paper
• Fibrin matrix-supported three-dimensional organ culture of adipose tissue for selective outgrowth, expansion, and isolation of adipose-derived stem cells.

  2011cites this paper
• A macrophage migration inhibitory factor‐like tautomerase from Teladorsagia circumcincta (Nematoda: Strongylida)

  2010cites this paper
• Immunological studies on the drug-induced allergic hepatitis

  2008cites this paper
• Immunological studies on drug-induced allergic hepatitis

  2007cites this paper
• ErbB‐4 may control behavior of prostate cancer cells and serve as a target for molecular therapy

  2007cites this paper
• Immunological studies on drug-induced allergic hepatitis

  2007cites this paper
• Structure of three isomeric host-specific toxins fromHelminthosporium sacchari

  2005cites this paper
• MURINE MACROPHAGES AND PANCREATIC 0 CELLS Chemotactic Properties of Insulin and , 8-Cytostatic Action of Interleukin

  2003cites this paper
• Hypersensitivity to metallic biomaterials: a review of leukocyte migration inhibition assays.

  2000cites this paper
• Erythrogenic toxin type B and its precursor isolated from nephritogenic streptococci induce leukocyte infiltration in normal rat kidneys.

  1999cites this paper
• Biological behavior and cell properties of new AKR lymphoma malignancy variants.

  1998cites this paper
• High-yield expression and purification of recombinant human macrophage migration inhibitory factor.

  1997cites this paper
• Metastasis-related cell functions in primary and metastatic tumor cells of AKR lymphoma.

  1996cites this paper
• Lymphocyte stimulation test with tetrazolium-based colorimetric assay for diagnosis of drug-induced allergic hepatitis.

  1994cites this paper
• Alpha-naphthyl acetate esterase-1 (ANAE-1) secreted by epithelioid cells from induced rabbit lung granuloma showed MIF activity.

  1992cites this paper
• Rat liver protein linking chemical and immunological detoxification systems

  1992cites this paper
• FGF Promotes Corneal Stromal Fibroblast Motility

  1992cites this paper
• Killing of Paracoccidioides brasiliensis conidia by pulmonary macrophages and the effect of cytokines.

  1992cites this paper
• Effects of dialyzable lymph node extracts on lymphoblast proliferative capacity of blood mononuclear cells in cattle with chronic paratuberculosis.

  1992cites this paper
• Differential expression of migration inhibitory and migration stimulatory factors in two lines of mice genetically selected for high or low responsiveness to phytohemagglutinin. 1. Migration stimulatory factor(s) from T and B cells of immune spleen.

  1991cites this paper
• Differential expression of migration inhibitory and migration stimulatory factors in two lines of mice genetically selected for high or low responsiveness to phytohemagglutinin. 2. Effects of mitogenic or allogeneic stimulation.

  1991cites this paper
• Macrophage Migration Inhibitory Factor (MIF) Produced by a Human T Cell Hybridoma Clone

  1991cites this paper
• Macrophage migration inhibitory factor (MIF) produced by a human T cell hybridoma clone.

  1991cites this paper
• Migration inhibition factor in acute serum sickness nephritis.

  1990cites this paper
• Effects of 5-fluorouracil and mitomycin C on cultured rabbit subconjunctival fibroblasts.

  1990cites this paper
• Leukozytenmigrationshemmung nach experimenteller Trypanosomeninfektion beim Rind

  1989cites this paper
• Regulation of lymphokine response during reinfection by influenza virus. Production of a factor that inhibits lymphokine activity.

  1989cites this paper
• Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor.

  1989cites this paper
• EGF does not enhance corneal epithelial cell motility.

  1989cites this paper
• Monocyte‐modulating Activities in the Sera of Patients with Granuloma Annulare

  1988cites this paper
• Generation, Biology, and Assay of Efferent Lymphokines

  1988cites this paper
• Migratory behavior of cells on embryonic retina basal lamina.

  1988cites this paper
• Proceedings of the Task Force on Guidelines for Standardizing Old and New Technologies Used for the Diagnosis and Treatment of Allergic Diseases. Washington, DC. June 18-19, 1987.

  1988cites this paper
• The Pharmacology of Lymphocytes

  1988cites this paper
• A rapid computerized method for quantitation of cellular mediated immunity in biological systems.

  1988cites this paper
• A rapid photoelectric method for reading human leukocyte migration

  1987cites this paper
• A new assay to measure monoclonal antibody-dependent complement or macrophage-mediated tumor growth inhibition.

  1987cites this paper
• Reactivation of herpes simplex virus is associated with production of a low molecular weight factor that inhibits lymphokine activity in vitro.

  1987cites this paper
• Stress-related immune suppression: health implications.

  1987cites this paper
• Murine macrophages and pancreatic beta cells. Chemotactic properties of insulin and beta-cytostatic action of interleukin 1

  1987cites this paper
• Monocyte activating factor in sarcoidosis. I. Existence of the factor in sarcoidosis sera.

  1986cites this paper
• Leukocyte migration inhibition in bacillus Calmette-Guérin vaccinated healthy adults and in tuberculosis assayed by a rapid photoelectric procedure.

  1986cites this paper
• Lymphokine activity in coelenterate envenomation.

  1986cites this paper
• A Monoclonal Antibody Against Migration Inhibitory Factor (MIF) Obtained by Immunization With MIF From the Human Lymphoblast Cell Line Mo

  1986cites this paper
• Characterization of three different rat T-cell clones with specificity to Listeria monocytogenes: phenotype, specific proliferation, lymphokine production, and protective capacity in vivo.

  1986cites this paper
• Monocyte procoagulant inducing factor: a lymphokine involved in the T cell-instructed monocyte procoagulant response to antigen.

  1986cites this paper
• Parameters of T cell mediated immunity to commensal micro-organisms in patients with chronic purulent rhinosinusitis: a comparison between delayed type hypersensitivity skin test, lymphocyte transformation test and macrophage migration inhibition factor assay.

  1986cites this paper
• Cell‐mediated immune responses to artificial food additives in chronic urticaria

  1986cites this paper
• Immunopotentiating activities of cell walls, peptidoglycans, and teichoic acids from two strains of Listeria monocytogenes

  1986cites this paper
• Monocyte activating factor in sarcoidosis. II. Secretion of the factor from monocytes.

  1986cites this paper
• Cellular mediators of anti-histoplasma immunity: II. Protective immunity and delayed hypersensitivity in mice immunized by sublethal infection with yeast cells of histoplasma capsulatum.

  1986cites this paper
• Simple methods for the assessment of migration areas in lymphocyte migration under agarose.

  1986cites this paper
• T cell-derived migration-inhibitory factor and colony-stimulating factor share common structural elements.

  1986cites this paper
• [28] Migration inhibitory factor

  1985cites this paper
• Cytomegalovirus (CMV): specific lymphokine production in congenital CMV infection.

  1985cites this paper
• Application of migration inhibition techniques in tumor immunology.

  1985cites this paper
• Generation of human hybridomas producing migration inhibitory factor (MIF) and of murine hybridomas secreting monoclonal antibodies to human MIF.

  1985cites this paper
• Selection of Human T-Cell Hybridomas That Produce Inflammatory Lymphokines by the Emetine-Actinomycin D Method

  1985cites this paper
• The Generation of Stable Human T-Cell Hybridomas Which Constitutively Produce Interleukin-2 and Chemotactic Factor

  1985cites this paper
• A reappraisal of the macrophage electrophoretic mobility (MEM) test for the measurement of lymphokine activity.

  1985cites this paper
• Immune responses during human Schistosomiasis mansoni. XI. Immunologic status of patients with acute infections and after treatment.

  1985cites this paper
• MIF-like activity of natural and recombinant human interferon-gamma and their neutralization by monoclonal antibody.

  1985cites this paper
• Migration inhibitory factor.

  1985cites this paper
• Immunogenicity and Protective Effect of Hemolysis Mutants of Mycoplasma pneumoniae

  1985cites this paper
• Role of interleukin 1 in experimental pulmonary granuloma in mice.

  1985cites this paper
• Human Hybridomas and Monoclonal Antibodies

  1985cites this paper
• Correlation of macrophage migration-inhibition factor and protection from challenge exposure in calves vaccinated with Salmonella typhimurium.

  1985cites this paper
• Macrophage migration inhibition test of peritoneal exudate cells of Litomosoides carinii-infected cotton rats by direct method in agar plate.

  1985cites this paper
• A CASE OF GRANULOMA ANNULARE AND SARCOIDOSIS

  1985cites this paper
• Strain variation of bacillus Calmette-Guerin-induced pulmonary granuloma formation is correlated with anergy and the local production of migration inhibition factor and interleukin 1.

  1985cites this paper
• Cellular immune responses in mice challenged with an amyocarditic variant of coxsackievirus B3

  1985cites this paper
• Demonstration of leukocyte inhibitory factor in human Schistosomiasis mansoni and its evaluation from patients in an endemic setting.

  1985cites this paper
• Functional characteristics of murine macrophages responding to migration inhibitory factors

  1984cites this paper
• Restorative effects of thymosin polypeptides on purified protein derivative--dependent migration inhibition factor production by the peripheral blood lymphocytes of adult thymectomized guinea pigs.

  1984cites this paper
• Dissociation of human interferon-gamma-like activity from migration-inhibition factor.

  1984cites this paper
• Properties of TAS‐1D3, a Tuberculin‐Active Substance from BCG, in Regard to Delayed Hypersensitivity

  1984cites this paper
• A method using [3H]leucine-labeled granulocytes or macrophages for migration inhibition assay.

  1984cites this paper
• Gamma interferon production by peripheral blood lymphocytes in patients with gastric cancer.

  1984cites this paper
• Macrophage-activating factor for cytotoxicity produced by a human T-cell hybridoma.

  1984cites this paper
• Cell-mediated immune responsiveness to cardiac extracts by peripheral blood leukocytes from patients after myocardial infarction or open-heart surgery.

  1984cites this paper
• Production of macrophage-activating and migration-inhibition factors in vitro by serologically selected and cloned Listeria monocytogenes-specific T cells of the Lyt 1+2- phenotype

  1984cites this paper
• Fetal gastric and colonic implants in syngeneic and allogeneic mice developing typical inflammatory changes

  1983cites this paper
• Delayed hypersensitivity and migration inhibition in two lines of mice genetically selected for high or low responsiveness to phytohemagglutinin.

  1983cites this paper
• An ELISA method for quantitation of macrophage migration from agarose microdroplets.

  1983cites this paper
• Human T-cell hybridomas producing migration inhibitory factor and macrophage activating factors.

  1983cites this paper
• Leukocyte migration inhibition by Mu-MTV-enriched extracts in breast cancer patients

  1983cites this paper
• Lymphokine-stimulated macrophage phagocytosis of fluorescent microspheres: a rapid new assay.

  1983cites this paper
• Lymphokine-producing human T-cell hybridomas.

  1983cites this paper
• A system for obtaining large numbers of cryopreserved human monocytes purified by leukapheresis and counter-current centrifugation elutriation (CCE).

  1983cites this paper
• Utilization of purified human monocytes in the agarose droplet assay for measuring migration inhibitory factors.

  1983cites this paper
• Cell-mediated immune injury to the heart.

  1983cites this paper
• Characterization of a human blood monocyte subset with low peroxidase activity.

  1983cites this paper
• Regulatory mechanisms of cutaneous delayed-type hypersensitivity. I. Suppression of cutaneous delayed-type hypersensitivity by migration inhibitory factor.

  1983cites this paper
• Acrylamide gel analysis of sucrose gradient-derived RNA B fractions transferring delayed sensitivity in vitro.

  1983cites this paper
• Human T-cell hybridomas producing lymphokines. II. Enhancement of lymphotoxin secretion from human T-cell hybridomas by phorbol myristate acetate.

  1983cites this paper