Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device

Abstract. High-throughput optical imaging is critical to obtain large-scale neural connectivity information of brain in neuroscience. Using a digital mirror device and a scientific complementary metal-oxide semiconductor camera, we report a significant speed improvement of structured illumination microscopy (SIM), which produces a maximum SIM net frame rate of 133 Hz. We perform three-dimensional (3-D) imaging of mouse brain slices at diffraction-limited resolution and demonstrate the fast 3-D imaging capability to a large sample with an imaging rate of 6.9×107  pixel/s of our system, an order of magnitude faster than previously reported.

• Publication year

  2013

• Venue

  Journal of Biomedical Optics

• Publication date

  2013-06-01

• Fields of study

  Materials Science, Medicine, Physics, Engineering

• Identifiers
  DOI 10.1117/1.JBO.18.6.060503PMID 23757041
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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