Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging

Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.

• Publication year

  2011

• Venue

  Nature Methods

• Publication date

  2011-12-27

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1038/nmeth.1854PMID 22245809PMCID 3297424
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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