Keratinase in starter diets improves growth of broiler chicks.

The objective of this study was to determine the efficacy of a broad-spectrum protease enzyme, PWD-1 keratinase, upon supplementation to corn-soy starter diets on growth performance of broiler chickens. Three experiments were conducted. In each experiment, 1-d-old broiler chicks were randomly assigned to 24 cage pens of eight birds per pen in a completely randomized design of five experimental treatments and grown to 21 or 26 d of age. Treatments in experiments 1 and 2 were control (C, 21.39% CP), low protein (LP, 18% CP), and LP supplemented with 0.05, 0.1, or 0.15% enzyme preparation (wt/wt). Treatments in experiment 3 were control (C), C+ 0.1% enzyme preparation (C+E) fed starting at either 1 or 5 d of age, LP and LP+ 0.1% enzyme preparation (LP+E). Feeding the LP+E diet produced numerically higher BW at 21 d of age (experiments 1 and 3) and a significantly higher BW at 26 d of age (experiment 2; 1,025 and 1,032 g vs. 965 g for 0.1 and 0.15% vs. LP, respectively, P < 0.05). Feed conversion ratio (FCR) was also improved when chicks were fed the LP+E diet both at 21 (experiment 3) and 26 d of age (experiment 2). In experiment 3, supplementing the C diets with 0.10% enzyme resulted in improvements (P < 0.05) in BW whether the enzyme was supplemented starting at 1 d (767 vs. 695 g for C+E vs. C, respectively) or 5 d of age (764 vs. 695 g for C+E vs. C, respectively). FCR was numerically improved. Furthermore, diets supplemented with the enzyme at any level resulted in reduction of jejunal viscosity at 22 and 27 d of age (P < 0.05). Results of these experiments indicate that the growth of broiler chickens can be significantly improved by dietary supplementation with PWD-1 keratinase.

• Publication year

  2003

• Venue

  Poultry Science

• Publication date

  2003-04-01

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology

• Identifiers
  DOI 10.1093/PS/82.4.664PMID 12710489
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Dietary Supplementation of Endoxylanases and Phospholipase for Turkeys Fed Wheat-based Rations

  2004cited by this paper
• Effect of wheat enzyme preparation on the performance characteristics of Tom turkeys fed wheat-based rations.

  2002cited by this paper
• Identification of ovotransferrin as an acute phase protein in chickens.

  2002cited by this paper
• Effect of mannan-endo-1,4-beta-mannosidase on the growth performance of turkeys fed diets containing 44 and 48% crude protein soybean meal.

  2002cited by this paper
• Fermentation production of keratinase from Bacillus licheniformisPWD-1 and a recombinant B. subtilis FDB-29

  1999cited by this paper
• Posthatch development of small intestinal function in the poult.

  1999cited by this paper
• Effects of diet formulation and enzyme inclusion on apparent metabolisable energy (AME) concentration in wheat-based diets and on broiler performance.

  1999cited by this paper
• Nutrient requirements of poultry

  1995cited by this paper
• Enzymes in the animal-feed industry.

  1993cited by this paper
• Purification and Characterization of a Keratinase from a Feather-Degrading Bacillus licheniformis Strain

  1992cited by this paper
• influence of dietary xylanase on intestinal viscosity and molecular weight distribution of carbohydrates in rye-fed broiler chicks

  1991cited by this paper
• Evaluation of three enzymic methods as predictors of in-vivo response to enzyme supplementation of barley-based diets when fed to young chicks

  1990cited by this paper
• Isolation, Identification, and Characterization of a Feather-Degrading Bacterium

  1990cited by this paper
• Enzyme supplementation of a poultry diet containing rye and wheat

  1989cited by this paper
• The effect of heat treatment and enzyme supplementation on the nutritive value of barley for broiler chicks

  1975cited by this paper
• Improvement in the Nutritional Value of Barley for Chicks by Enzyme Supplementation

  1957cited by this paper

• Nutritional and Microbiome Effects of a Partial Substitution of Poultry Meat with Hydrolyzed Feather Meal in Dog Diets

  2025cites this paper
• Keratinous bioresources: their generation, microbial degradation, and value enhancement for biotechnological applications

  2025cites this paper
• A Highly Active Keratinase from Bacillus sp. FJ-3-16 for Sustainable Feather Waste Valorization and Eco-Friendly Industrial Applications

  2025cites this paper
• Transforming Feather Meal Into a High‐Performance Feed for Broilers

  2025cites this paper
• Structural and enzymatic characterization of a novel metallo-serine keratinase KerJY-23.

  2024cites this paper
• Molecular strategies to enhance the keratinase gene expression and its potential implications in poultry feed industry

  2024cites this paper
• Extraction of Keratin from Chicken Feathers and its Application in the Treatment of Contaminated Water: an Eco-Friendly Approach

  2024cites this paper
• UJI KATALITIK ENZIM KERATINASE, LIPASE DAN SELULASE ISOLAT ACTINOMYECTES SD-5 DARI PERAIRAN PANTAI MANGROVE OESAPA, KUPANG YANG BERPOTENSI DIKEMBANGKAN SEBAGAI IMBUHAN PAKAN MULTIENZIM

  2024cites this paper
• In vitro and in vivo evaluation of the nutritional value of various hydrolyzed feather meals and comparison of their effect on performance and carcass characteristics of broilers

  2024cites this paper
• Harnessing the potential of microbial keratinases for bioconversion of keratin waste

  2024cites this paper
• Interactions of wheat cultivars and supplemental enzyme in diet of broiler chickens on growth performance, carcass characteristics, intestinal morphometric features, and blood parameters

  2024cites this paper
• Isolation, identification, and molecular characterization of potential keratinolytic fungus sp. from Southern Assam: relevance to poultry wastes and its biological management

  2024cites this paper
• Current Progress and Biotechnological Applications of Microbial Keratinases

  2023cites this paper
• Sustainable utilization of feather waste to produce alkaline serine protease by genetically engineered Bacillus amyloliquefaciens

  2023cites this paper
• Valorization of chicken feather into organic liquid fertilizer through two species of Bacillus bacteria fermentation

  2023cites this paper
• Histomorphometric traits, microbiota, nutrient digestibility, growth performance, carcass traits and meat quality parameters of chickens fed diets supplemented with different levels of Bacillus protease

  2023cites this paper
• Biochemical and molecular characterization of novel keratinolytic protease from Bacillus licheniformis (KRLr1)

  2023cites this paper
• Insights into the keratin efficient degradation mechanism mediated by Bacillus sp. CN2 based on integrating functional degradomics

  2023cites this paper
• Effects of protease supplementation on growth performance, organ development, gut morphology, and microbial profile of broiler chicken

  2022cites this paper
• Interactive effect of amino acids balanced at ideal lysine ratio and exogenous protease supplemented to low CP diet on growth performance, carcass traits, gut morphology, and serum metabolites in broiler chicken

  2022cites this paper
• Keratinase improves the growth performance, meat quality and redox status of broiler chickens fed a diet containing feather meal

  2022cites this paper
• ISOLATION AND SCREENING OF KERATINSE PRODUCING BACTERIA FROM CHICKEN FARM BED

  2022cites this paper
• Kinetics and thermodynamics of keratin degradation by partially purified and encapsulated keratinase from Bacillus vallismortis DSM11031

  2021cites this paper
• Isolation and Identification of Feather Degrading Bacteria from Poultry Waste Dump site along Enugu-Port Harcourt Express way, Gariki, Awkunanaw, Enugu.

  2021cites this paper
• Proteases in the diet of monogastric animals

  2021cites this paper
• Feather Biodegradation For Feather Protein Lysate/ Feather Meal Mutual Formations: Optimization Using Crude YNDH Protease/Keratinase; Enzyme Partial Purification and Characterization

  2021cites this paper
• Significance of keratinophiles in biofertilizer development from keratinous waste: Upcoming perspective

  2021cites this paper
• Microbial Hydrolysed Feather Protein as a Source of Amino Acids and Protein in the Diets of Animals Including Poultry

  2021cites this paper
• Feather protein lysate optimization and feather meal formation using YNDH protease with keratinolytic activity afterward enzyme partial purification and characterization

  2021cites this paper
• The effect of a heat-stable xylanase on digesta viscosity, apparent metabolizable energy and growth performance of broiler chicks fed a wheat-based diet

  2021cites this paper
• Dietary supplementation of a microencapsulated essential oil-organic acid blend with and without a protease affects performance, nutrient accretion, cecal microbiota, and circulatory biomarker concentrations in male broiler chickens

  2021cites this paper
• Sustainable production, biochemical and molecular characterization of thermo-and-solvent stable alkaline serine keratinase from novel Bacillus pumilus ar57 for promising poultry solid waste management.

  2020cites this paper
• Effect of dietary supplementation of microbial protease on performance, egg quality and nitrogen retention in white leghorn layers

  2020cites this paper
• Enhancing Plant Growth by Chicken Feather Compost Obtained from Feather Degradation  by Streptomyces enissocaesili

  2020cites this paper
• Effective biodegradation of chicken feather waste by co-cultivation of keratinase producing strains

  2019cites this paper
• Enzymes in the Animal Feed Industry

  2019cites this paper
• Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease

  2019cites this paper
• Evaluating the Effect of Feed Mixing and Sampling Methodologies on Nutrient Analysis and the Inclusion of a Novel Protease in Broiler Diets

  2019influential citation
• Isolation, Molecular Identification and Verification of Gene Encoding Bacterial Keratinase from Crocodile (Crocodylus porosus) Feces

  2019cites this paper
• Using Protease and Xylanase Enzymes as Single or Combined Supplementation to Corn-Soybean Meal Broiler Diets

  2019influential citation
• Efficacy of a Mono-Component Exogenous Protease in the Presence of a High Concentration of Exogenous Phytase on Growth Performance of Broiler Chickens

  2019cites this paper
• Enhanced stability of immobilized keratinolytic protease on electrospun nanofibers

  2019cites this paper
• Evaluation of novel protease enzymes on growth performance and nutrient digestibility of poultry: enzyme dose response.

  2019cites this paper
• Isolation and characterization of keratinolytic bacteria from soil samples of poultry waste dumping sites.

  2018cites this paper
• Effect of keratinase on ileal amino acid digestibility in five feedstuffs fed to growing pigs

  2018cites this paper
• Versatility and commercial status of microbial keratinases: a review

  2018cites this paper
• A mono-component microbial protease improves performance, net energy, and digestibility of amino acids and starch, and upregulates jejunal expression of genes responsible for peptide transport in broilers fed corn/wheat-based diets supplemented with xylanase and phytase

  2018cites this paper
• Meta‐analysis: explicit value of mono‐component proteases in monogastric diets

  2018cites this paper
• Avaliação da digestibilidade do concentrado proteico de soja associado a enzima protease em rações para frangos de corte nas fases pré-inicial e inicial

  2017cites this paper
• The Effect of Different Enzyme Substrates on the Performance of Broilers Fed Peeled Cassava Root Meal (Pcrm) Based Diet

  2017cites this paper
• Interactive effects of dietary protein concentration, a mono-component exogenous protease and ascorbic acid on broiler performance, nutritional status and gut health

  2017cites this paper
• Effect of Combination between Phytase , Xylanase and Protease Enzymes in Different Growing Phases on Growth Performance and Carcass Characteristics of Broiler Chicks.

  2017cites this paper
• Feather Degradation and Keratinase Production by Bacillus Sp. and Lactobacillus Sp.

  2017cites this paper
• Microbial keratinases: industrial enzymes with waste management potential

  2017cites this paper
• Valorization of keratin based waste

  2017cites this paper
• Purification and characterization of a keratinolytic protease produced by probiotic Bacillus subtilis

  2016cites this paper
• SHG10 keratinolytic alkaline protease from Bacillus licheniformis SHG10 DSM 28096: Robust stability and unusual non‐cumbersome purification

  2016cites this paper
• Effect of carbohydrase and protease on growth performance and gut health of young broilers fed diets containing rye, wheat, and feather meal

  2016cites this paper
• Isolation and characterization of feather degrading bacteria from poultry waste

  2016cites this paper
• Isolation and identification of keratinolytic bacteria from poultry waste and assessment of its keratinase activity on chicken feathers

  2016cites this paper
• Isolation and characterization of feather degrading bacteria from poultry waste

  2016cites this paper
• Toward optimal value creation through the application of exogenous mono-component protease in the diets of non-ruminants

  2016cites this paper
• oward optimal value creation through the application of xogenous mono-component protease in the diets of on-ruminants

  2016cites this paper
• Isolation and Identification of Keratinolytic Bacteria that Exhibit Feather-degrading Potentials

  2016cites this paper
• Exploration of the Potent Bacterial Strain for the Application of Feather Degradation

  2016cites this paper
• Microbial enzymes for bioconversion of poultry waste into added-value products

  2015cites this paper
• Production and Characterization of Keratinolytic Proteases Produced by Onygena corvina

  2015cites this paper
• Effects of a Monocomponent Protease on Performance Parameters and Protein Digestibility in Broiler Chickens

  2015cites this paper
• Synthesis and swelling behaviors of semi-IPNs superabsorbent resin based on chicken feather protein

  2014cites this paper
• Exogenous enzymes in nutrient digestion and health of non-ruminant : Carbohydrases enzyme

  2014cites this paper
• Substitusi Konsentrat Protein Menggunakan Tepung Bulu Ayam yang Diolah Secara Fisiko-Kimia dan Fermentasi Menggunakan Bacillus sp. Mts

  2014cites this paper
• 羽毛分解微生物之角蛋白 ? 〞滲S性研究 = Study on characterization of keratinases from feather-degrading microorganisms

  2014cites this paper
• Effect of common feed enzymes on nutrient utilization of monogastric animals

  2014cites this paper
• Effects of Novel Feed Ingredients and Additives on Feed Quality and Broiler Performance

  2014cites this paper
• Growth performance, blood parameters, immune response and carcass traits of broiler chicks fed on graded levels of wheat instead of corn without or with enzyme supplementation.

  2014cites this paper
• The nutritional value for poultry and pigs of biofuel co-products

  2014cites this paper
• Practical Strategies to Maximize Cockatiel Health and Broiler Chicken Performance

  2014cites this paper
• Quality of Chicken Feather Processed in Different Conditions

  2014cites this paper
• Complete replacement of soybean meal in pig diets with hydrolyzed feather meal with blood by amino acid supplementation based on standardized lleal amino acid digestibility

  2014cites this paper
• A current assessment on the production of bacterial keratinases

  2014cites this paper
• Purification and characterization of a keratinolytic serine protease from Purpureocillium lilacinum LPS # 876

  2013cites this paper
• KECERNAAN LEMAK DAN ENERGI KONSENTRAT MONOGASTRIK BERBASIS HIDROLISAT TEPUNG BULU AYAM SECARA IN VITRO

  2013cites this paper
• EFFECT OF SUPPLEMENTING SOME GROWTH POROMOTERS TO DUCKLING DIETS ON GROWTH PERFORMANCE AND CARCASS TRAITS UNDER SUMMER CONDITION

  2013cites this paper
• Microbial Keratinases: Diversity and Applications

  2013cites this paper
• - 1-Identification and characterisation of a Bacillus licheniformis strain with profound keratinase activity for degradation of melanised feather

  2013cites this paper
• Isolation of a feather-degrading Bacillus subtilis strain from the alimentary tract of grebes

  2013cites this paper
• Complete Replacement of Soybean Meal in Pig Diets with Hydrolyzed Feather Meal with Blood by Amino Acid Supplementation Based on Standardized Ileal Digestibility

  2013influential citation
• Disulfide Bridging the Gap between Src and Cortactin: A New Paradigm in SH2 Domain-mediated Signaling

  2013cites this paper
• Keratinolytic proteases in biodegradation of pretreated feathers

  2013cites this paper
• Biotechnological applications and prospective market of microbial keratinases

  2013cites this paper
• Associação de enzimas em rações para frangos de corte

  2013cites this paper
• Steam-pelleting temperatures, grain variety, feed form and protease supplementation of mediumly ground, sorghum-based broiler diets: influences on growth performance, relative gizzard weights, nutrient utilisation, starch and nitrogen digestibility

  2013cites this paper
• Isolation and characterization of feather degrading bacteria from Mauritian soil

  2012cites this paper
• Decomposition of Insoluble and Hard-to-Degrade Animal Proteins by Enzyme E77 and Its Potential Applications

  2012cites this paper
• Anaerobic digestion of chicken feather with swine manure or slaughterhouse sludge for biogas production.

  2012cites this paper
• Productive Performance, Intestinal Morphology and Carcass Yield of Broilers Fed Conventional and Alternative Diets Containing Commercial Enzymatic Complex

  2012cites this paper
• Effects of dietary supplementation of multi-enzyme complex on the energy utilization in rooster and performance of broiler chicks

  2012cites this paper
• Production and Optimization of Feather Protein Hydrolysate from Bacillus Sp. MPTK6 and Its Antioxidant Potential

  2012cites this paper
• Enzymatic degradation of prion protein by keratinase producing proteolytic micro-organisms

  2012cites this paper
• From biogas energy, biotechnology to new agriculture

  2012cites this paper