Neutrophil aggregation and swelling induced by chemotactic agents.

An assay is described for measuring neutrophil aggregation and cell size with a Coulter Counter equipped with a mean cell volume computer and volume channalyzer. With this assay, it was found that several chemotactically active agents rapidly induce rabbit peritoneal polymorphonuclear neutrophils in suspension to aggregate reversibly. The aggregating activities of these chemotactic agents paralleled their in vitro chemotactic- and in vivo neutropenia-inducing potencies. Neutrophil aggregation may underlie the neutropenia which follows the infusion of these agents into rabbits. Chemotactic agents may also induce polymorphonuclear neutrophils to increase in volume; this effect appears to be independent of aggregation. The volume change develops more slowly and does not appear reversible under the conditions employed. The volume increase may be an expression of pseudopodia formation and membrane ruffling known to develop in neutrophils exposed to chemotactic agents.

• Publication year

  1977

• Venue

  Journal of Immunology

• Publication date

  1977-07-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.4049/jimmunol.119.1.232PMID 874320
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• The observed volume increase may reflect pseudopodia formation and membrane ruffling in neutrophils exposed to chemotactic agents.

  Confidence 0.78

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• Chemotactic agents also cause a slower increase in polymorphonuclear neutrophil volume that appears independent of aggregation and does not reverse under the conditions used.

  Confidence 0.93

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• Several chemotactically active agents rapidly induce rabbit peritoneal polymorphonuclear neutrophils to aggregate reversibly, and their aggregating activity parallels both chemotactic potency and neutropenia-inducing potency.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• A Coulter Counter equipped with a mean cell volume computer and volume channalyzer can be used to measure neutrophil aggregation and cell size in suspension.

  Confidence 0.96

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review

• cell size

  measurement attribute

  The physical volume of neutrophils as measured in this assay.

  Aliases: cell volume, volume

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• chemotactic agents

  compound

  Compounds that attract neutrophils and are tested here for effects on aggregation and volume.

  Aliases: chemotactically active agents

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• coulter counter

  instrument

  An electronic cell-counting instrument used here to measure neutrophil aggregation and size-related changes.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• mean cell volume computer

  device

  A Coulter Counter accessory that calculates mean cell volume from the measured cell population.

  Aliases: MCV computer

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• membrane ruffling

  cell process

  Surface membrane folding or ruffling seen in activated neutrophils.

  Aliases: ruffling

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• neutropenia

  condition

  A reduction in circulating neutrophils, considered here as an in vivo response associated with the tested agents.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• neutrophil aggregation

  cell behavior

  The clustering of neutrophils in suspension into reversible aggregates.

  Aliases: aggregation

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• pseudopodia formation

  cell process

  The extension of protrusive cell projections associated with activated neutrophils.

  Aliases: pseudopod formation

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• rabbit peritoneal polymorphonuclear neutrophils

  cell type

  Rabbit neutrophils collected from the peritoneal cavity and used as the cell suspension in the assay.

  Aliases: polymorphonuclear neutrophils, rabbit neutrophils, PMNs

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review
• volume channalyzer

  device

  A Coulter Counter attachment that sorts cells into volume channels for size analysis.

  Aliases: volumetric channalyzer

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewmexicorea (qjvnbu8xg3) reviewAnonymous (b2adb6bfad) review

• Mechanism of Anti-Complementary Activity of Corticosteroids in Vivo: Possible Relevance in Endotoxin Shock

  1977cited by this paper
• The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal secretion for neutrophils

  1976cited by this paper
• Role of Ca2+ and Mg2+ in some human neutrophil functions as indicated by ionophore A23187

  1976cited by this paper
• Purification and partial characterization of human and porcine C3a anaphylatoxin.

  1975cited by this paper
• Volume changes induced in rabbit polymorphonuclear leukocytes by chemotactic factor and cytochalasin B.

  1975cited by this paper
• Lysosomal Enzyme Secretion and Volume Contraction Induced in Neutrophils by Cytochalasin B, Chemotactic Factor and A23187 1 2

  1975cited by this paper
• The aggregation of rabbit polymorphonuclear leucocytes: effects of antimitotic agents, cyclic nucleotides and methyl xanthines.

  1974cited by this paper
• THE REGULATORY ROLE OF DIVALENT CATIONS IN HUMAN GRANULOCYTE CHEMOTAXIS

  1974cited by this paper
• New biological activity following intravascular activation of the complement cascade

  1974cited by this paper
• Colchicine and Vinblastine Inhibit Fibroblast Aggregation

  1974cited by this paper
• The ability of chemotactic factors to induce lysosomal enzyme release. I. The characteristics of the release, the importance of surfaces and the relation of enzyme release to chemotactic responsiveness.

  1974cited by this paper
• LEUKOCYTE LOCOMOTION AND CHEMOTAXIS

  1973cited by this paper
• FORMATION OF C3a AND C5a ANAPHYLATOXINS IN WHOLE HUMAN SERUM AFTER INHIBITION OF THE ANAPHYLATOXIN INACTIVATOR

  1973cited by this paper
• The effect of Ca2+ and Mg2+ on the chemotactic responsiveness and spontaneous motility of rabbit polymorphonuclear leukocytes.

  1972cited by this paper
• The effect of neuraminidase on the aggregation of BHK21 cells and BHK21 cells transformed by polyoma virus.

  1972cited by this paper
• The aggregation of trypsinized BHK21 cells.

  1971cited by this paper
• Intercellular adhesion

  1969cited by this paper
• THE ROLE OF SERUM COMPLEMENT IN CHEMOTAXIS OF LEUKOCYTES IN VITRO

  1965cited by this paper

• Mechano-induced homotypic patterned domain formation by monocytes

  2023cites this paper
• Mechano-induced homotypic patterned domain formation by monocytes

  2023cites this paper
• Interleukin 8 Elicits Rapid Physiological Changes in Neutrophils That Are Altered by Inflammatory Conditions

  2021cites this paper
• What Is the Evolutionary Fingerprint in Neutrophil Granulocytes?

  2020cites this paper
• The role of formyl peptide receptors in the regulation of platelet function

  2019cites this paper
• Leukocytes: Gradient Fractionation and Relationship to Chemotactic Stimulation

  2016cites this paper
• Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

  2016cites this paper
• Human whole-blood granulocyte aggregation in vitro 183

  2012cites this paper
• ----------- u 50-- I • • •---------- -2 S . D . Figure 2 . Chemotactic Responses of 24 Adults and 24 Neonates

  2012cites this paper
• WAKE FOREST UNIVERSITY SCHOOL OF MEDICINE CURRICULUM VITAE

  2011cites this paper
• Neutrophil aggregation--factors modulating stimulus-specific responses.

  2009cites this paper
• 1-0-A lkyl-sn-glyceyl-3-phosphoycholines A Novel Class of Neutrophil Stimulants

  2007cites this paper
• Mechanism of Action

  2007cites this paper
• Pulmonary Injury Induced by Phorbol Myristate Acetate Following Intravenous Administration in Rabbits Acute Respiratory Distress Followed by Pulmonary Interstitial Pneumonitis and Pulmonary Fibrosis

  2007cites this paper
• Gravity sedimentation of leukocytes is partially independent from erythrocyte sedimentation

  2006cites this paper
• Complement activation and HLA-B 27

  2004cites this paper
• Themotaxis of metastatic tumor cells

  2004cites this paper
• Anaplasma phagocytophilum Utilizes Multiple Host Evasion Mechanisms To Thwart NADPH Oxidase-Mediated Killing during Neutrophil Infection

  2004cites this paper
• Circulating neutrophil concentration and respiratory distress in premature infants.

  2000cites this paper
• The determination of the cellular volume of avian, porcine and bovine phagocytes and bovine mammary epithelial cells and its relationship to uptake of tilmicosin.

  1999cites this paper
• Expression of soluble endothelial adhesion molecules in clinical cardiopulmonary bypass

  1998cites this paper
• N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP) inhibits tumour necrosis factor‐alpha (TNF‐α) production on lipopolysaccharide (LPS)‐stimulated human neutrophils

  1998cites this paper
• Cell volume regulatory ion transport in cell migration.

  1998cites this paper
• Human recombinant interleukin-1β induces thromboxane A2 release in polymorphonuclear leukocytes, macrophages and platelets: Effect of IL-1 receptor antagonist

  1996cites this paper
• Enhanced aggregation of human neutrophils by MnCl2 or DTT differentiates the roles of L‐selectin and β2‐integrins

  1996cites this paper
• Protective effect of methionine in the ischemia-reperfusion cardiac injury in the canine model

  1996cites this paper
• Investigation of the signal transduction pathways involved in the induction of T-lymphocyte motility.

  1996cites this paper
• DTT differentiates the roles of L-selectin and 32-integrins

  1996cites this paper
• Effect of pyridoxal 5′-phosphate on human neutrophil aggregationin vitro

  1995cites this paper
• Characterization of eosinophil homotypic aggregation

  1995cites this paper
• Oxygenators for Extracorporeal Circulation

  1995cites this paper
• Complement and neutrophil activation during cardiopulmonary bypass: a study in the complement-deficient dog.

  1994cites this paper
• Migration-associated volume changes in neutrophils facilitate the migratory process in vitro.

  1994cites this paper
• The regulation of chemotaxis and chemokinesis in Dictyostelium amoebae by temporal signals and spatial gradients of cyclic AMP.

  1994cites this paper
• Regulation of human neutrophil aggregation: comparable latent times, activator sensitivities, and exponential decay in aggregability for FMLP, platelet-activating factor, and leukotriene B4.

  1993cites this paper
• Complement inhibition with soluble complement receptor type 1 in cardiopulmonary bypass.

  1993cites this paper
• Increased oxygen free radical activity in patients on cardiopulmonary bypass undergoing aortocoronary bypass surgery.

  1992cites this paper
• Assays of random motility of polymorphonuclear leukocytes in vitro.

  1992cites this paper
• Peri‐operative changes in plasma C3a and C5a concentrations in infants

  1992cites this paper
• Perioperative complement activation in neonates under halothane or fentanyl anaesthesia

  1992cites this paper
• Dynamics of Human Neutrophil Aggregation Evaluated by Flow Cytometry

  1991cites this paper
• Influence of endothelin on cardiovascular function, oxygen free radicals, and blood chemistry.

  1991cites this paper
• Studies on the adhesion and locomotion of neutrophil leucocytes

  1990cites this paper
• Comparative studies of microscopically determined aggregation, degranulation, and light transmission after chemotactic activation of adult and newborn neutrophils

  1990cites this paper
• Stimulation of human polymorphonuclear leukocytes potentiates the uptake of diclofenac and the inhibition of chemotaxis.

  1990cites this paper
• Effect of polymorphonuclear leukocyte-derived oxygen free radicals and hypochlorous acid on cardiac function and some biochemical parameters.

  1990cites this paper
• Relationship of Chemotactic Receptors for Formyl Peptide and C5a to CR1, CR3, and Fc Receptors on Human Neutrophils

  1990cites this paper
• Perioperative effect of methylprednisolone given during lung surgery on plasma concentrations of C3a and C5a.

  1990cites this paper
• Comparative studies of microscopically determined aggregation, degranulation, and light transmission after chemotactic activation of adult and newborn neutrophils.

  1990cites this paper
• Inhibition of neutrophil functions by scrapie prion protein: description of some inhibitory properties.

  1990cites this paper
• Alterations in arachidonic acid metabolism and chemotactic response in polymorphonuclear leukocytes from patients with rheumatoid arthritis.

  1989cites this paper
• Human whole-blood granulocyte aggregation in vitro.

  1989cites this paper
• Pitfalls in testing drug uptake by leukocytes using radiolabeled drugs and an explanation of conflicting results with clindamycin.

  1989cites this paper
• Functional and metabolic heterogeneity among normal neutrophil subpopulations

  1989cites this paper
• [Neutrophil-dependent inflammatory reactions in systemic lupus erythematosus].

  1989cites this paper
• Leucocyte Adhesion to Cells

  1989cites this paper
• Complement activation and HLA-B27.

  1988cites this paper
• Adherence, spreading, and locomotion of bovine polymorphs: effect of proteins and metabolic inhibitors.

  1988cites this paper
• Release of granulocyte proteins during cardiopulmonary bypass: effect of different pharmacological interventions.

  1988cites this paper
• [Effect of corticosteroids, quinacrine and L-652, 731 on experimental aggregation of polymorphonuclear leukocytes induced by complement and platelet activating factor (PAF)].

  1988cites this paper
• Chemotaxis: an overview.

  1988cites this paper
• Deposition of terminal C5b-9 complement complexes on erythrocytes and leukocytes during cardiopulmonary bypass.

  1988cites this paper
• Adherence of fresh and stored granulocytes to endothelial cells. Effect of storage temperature

  1988cites this paper
• A new simplified assay for evaluation of motile activity of human polymorphonuclear leucocytes.

  1988cites this paper
• Complement activation during cardiopulmonary bypass: comparison between the use of large volumes of plasma and dextran 70.

  1988cites this paper
• Assay for the leukotriene B4 receptor.

  1987cites this paper
• Biological activities of complement-derived peptides.

  1987cites this paper
• Intestinal absorption, metabolism and effects of bacterial chemotactic peptides in rat intestine

  1987cites this paper
• Destruction of cultured vascular endothelial cells and red blood cells by immune-activated polymorphonuclear leukocytes

  1987cites this paper
• The mechanisms and management of noncardiogenic pulmonary edema following cardiopulmonary bypass.

  1987cites this paper
• Hepatobiliary excretion and enterohepatic circulation of bacterial chemotactic peptide (FMLP) in the rat

  1987cites this paper
• Neutrophil emigration and microvascular injury. Role of chemotaxins, endotoxin, interleukin-1 and tumor necrosis factor alpha.

  1987cites this paper
• Differences and similarities between human and rabbit neutrophil granulocyte responses in vitro: the effects of zymosan‐activated plasma, phorbol myristate acetate and n‐formyl‐methionyl‐leucyl‐phenylalanine

  1987cites this paper
• Characterization of f-Met-Leu-Phe-stimulated fluid pinocytosis in human polymorphonuclear leukocytes by flow cytometry.

  1986cites this paper
• Neutrophil leucocyte chemotaxis is not induced by a spatial gradient of chemoattractant.

  1986cites this paper
• Production of peptides inducing chemotaxis and lysosomal enzyme release in human neutrophils by intestinal bacteria in vitro and in vivo.

  1986cites this paper
• Low density neutrophils in patients with systemic lupus erythematosus, rheumatoid arthritis, and acute rheumatic fever.

  1986cites this paper
• Volume changes in activated human neutrophils: The role of Na+/H+ exchange

  1986cites this paper
• A monoclonal antibody that induces T cell aggregation reacts with vascular endothelial cells and placental trophoblasts.

  1986cites this paper
• Density changes in leukocytes following hemodialysis or exposure to chemotactic factors.

  1986cites this paper
• Non-phagocytic beta-glucuronidase release in Behçet's disease.

  1986cites this paper
• The effect of body temperature on leukocyte kinetics during cardiopulmonary bypass.

  1985cites this paper
• Comparison of the effects of some compounds on human neutrophil degranulation and leukotriene B4 and thromboxane B2 synthesis.

  1985cites this paper
• Leukocyte-endothelial interactions.

  1985cites this paper
• Leukocyte Counts and Complement Activation During Arteriovenous and Pump-Driven Hemofiltration

  1985cites this paper
• Effects of bacterial endotoxins on neutrophil function.

  1985cites this paper
• Extracellular cytotoxicity by phagocytosing polymorphonuclear neutrophilic leukocytes: enhancement by a chemotactic stimulus.

  1985cites this paper
• The role of the polymorphonuclear leukocyte in the pathogenesis of the adult respiratory distress syndrome.

  1985cites this paper
• Adhesion of neutrophil leucocytes under conditions of flow.

  1984cites this paper
• Localization of chemotactic peptide receptors on rabbit neutrophils.

  1984cites this paper
• Chemoattraction and chemotaxis in Dictyostelium discoideum: myxamoeba cannot read spatial gradients of cyclic adenosine monophosphate

  1984cites this paper
• Leukotrienes: mediators of inflammation and immediate hypersensitivity reactions.

  1984cites this paper
• Renal cortical necrosis and Purtscher's retinopathy in hemorrhagic pancreatitis.

  1984cites this paper
• Chemotaxis of neutrophil granulocytes. Measurement, cell biology and clinical significance.

  1984cites this paper
• Inhibition of human polymorphonuclear leukocyte cell responses by ibuprofen.

  1984cites this paper
• Mechanisms of leukocyte regulation by complement-derived factors.

  1984cites this paper
• Pneumococcal-induced pulmonary leukostasis and hemodynamic changes: role of complement and granulocytes.

  1984cites this paper
• Physiological and pathological effects of activated complement.

  1984cites this paper
• Plasma lactoferrin reflects granulocyte activation via complement in burn patients.

  1984cites this paper
• Clinical disorders of leukocyte functions.

  1984cites this paper