A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

Proximity-dependent biotin identification (BioID) is a new approach making use of biotin ligase fusion proteins for the identification of both interacting and neighboring proteins in their native cellular environment.

• Publication year

  2012

• Venue

  Journal of Cell Biology

• Publication date

  2012-03-19

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1083/jcb.201112098PMID 22412018PMCID 3308701
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Nuclear lamins and laminopathies

  2012cited by this paper
• Nuclear lamina at the crossroads of the cytoplasm and nucleus.

  2012influential reference
• Biotinylation is a natural, albeit rare, modification of human histones.

  2011cited by this paper
• Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.

  2011cited by this paper
• Samp1 is functionally associated with the LINC complex and A-type lamina networks

  2011cited by this paper
• The nucleoskeleton as a genome-associated dynamic 'network of networks'

  2011cited by this paper
• Distinct association of the nuclear pore protein Nup153 with A- and B-type lamins

  2011cited by this paper
• Identification of differential protein interactors of lamin A and progerin

  2010cited by this paper
• Actin complexes in the cell nucleus: new stones in an old field

  2010cited by this paper
• Nuclei take a position: managing nuclear location.

  2009cited by this paper
• Laminopathies and the long strange trip from basic cell biology to therapy.

  2009cited by this paper
• Mutations of the FHL1 gene cause Emery-Dreifuss muscular dystrophy.

  2009cited by this paper
• Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization

  2009influential reference
• Barrier-to-Autointegration Factor Proteome Reveals Chromatin-Regulatory Partners

  2009cited by this paper
• Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.

  2008cited by this paper
• The A- and B-type nuclear lamin networks: microdomains involved in chromatin organization and transcription.

  2008cited by this paper
• A minimal peptide substrate in biotin holoenzyme synthetase‐catalyzed biotinylation

  2008cited by this paper
• Functional association of Sun1 with nuclear pore complexes

  2007influential reference
• Nesprin-1 and -2 are involved in the pathogenesis of Emery Dreifuss muscular dystrophy and are critical for nuclear envelope integrity.

  2007cited by this paper
• A versatile system for site-specific enzymatic biotinylation and regulated expression of proteins in cultured mammalian cells.

  2007influential reference
• Metabolic biotinylation of lentiviral pseudotypes for scalable paramagnetic microparticle-dependent manipulation.

  2006influential reference
• Mutant nuclear lamin A leads to progressive alterations of epigenetic control in premature aging.

  2006cited by this paper
• Nonenzymatic biotinylation of a biotin carboxyl carrier protein: Unusual reactivity of the physiological target lysine

  2006cited by this paper
• Multiple roles for emerin: implications for Emery-Dreifuss muscular dystrophy.

  2006cited by this paper
• Direct Binding of Nuclear Membrane Protein MAN1 to Emerin in Vitro and Two Modes of Binding to Barrier-to-Autointegration Factor*

  2005cited by this paper
• Codon optimization of the BirA enzyme gene leads to higher expression and an improved efficiency of biotinylation of target proteins in mammalian cells.

  2005cited by this paper
• Targeted and proximity-dependent promiscuous protein biotinylation by a mutant Escherichia coli biotin protein ligase.

  2005cited by this paper
• Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket.

  2004influential reference
• Promiscuous protein biotinylation by Escherichia coli biotin protein ligase

  2004cited by this paper
• Nuclear Membrane Proteins with Potential Disease Links Found by Subtractive Proteomics

  2003cited by this paper
• Transcriptional Repressor Germ Cell-less (GCL) and Barrier to Autointegration Factor (BAF) Compete for Binding to Emerin in Vitro *

  2003cited by this paper
• Direct interaction with nup153 mediates binding of Tpr to the periphery of the nuclear pore complex.

  2003influential reference
• Barrier-to-autointegration factor

  2002cited by this paper
• Nuclear lamins: building blocks of nuclear architecture.

  2002cited by this paper
• The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins

  2001cited by this paper
• Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells

  2001cited by this paper
• Distinct functional domains in emerin bind lamin A and DNA-bridging protein BAF.

  2001cited by this paper
• Identification of in vivo DNA targets of chromatin proteins using tethered Dam methyltransferase

  2000cited by this paper
• Clinical and molecular genetic spectrum of autosomal dominant Emery‐Dreifuss muscular dystrophy due to mutations of the lamin A/C gene

  2000cited by this paper
• Nuclear Lamins a and B1

  2000cited by this paper
• Multiple disordered loops function in corepressor-induced dimerization of the biotin repressor.

  2000cited by this paper
• Lamina-associated polypeptide 2alpha binds intranuclear A-type lamins.

  2000cited by this paper
• Nup50, a Nucleoplasmically Oriented Nucleoporin with a Role in Nuclear Protein Export

  2000cited by this paper
• Function of a conserved sequence motif in biotin holoenzyme synthetases

  2000cited by this paper
• Different mutations in the LMNA gene cause autosomal dominant and autosomal recessive Emery-Dreifuss muscular dystrophy.

  2000cited by this paper
• Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis.

  1999cited by this paper
• Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins.

  1999cited by this paper
• Loss of a-Type Lamin Expression Compromises Nuclear Envelope Integrity Leading to Muscular Dystrophy

  1999cited by this paper
• Mutations in the gene encoding lamin A/C cause autosomal dominant Emery-Dreifuss muscular dystrophy

  1999cited by this paper
• Molecular biology of biotin attachment to proteins.

  1999cited by this paper
• Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy

  1994cited by this paper
• A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm.

  1993cited by this paper
• Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation.

  1993cited by this paper
• Independent expression and assembly properties of heterologous lamins A and C in murine embryonal carcinomas.

  1992cited by this paper
• Free diffusion to and from the inner nuclear membrane of newly synthesized plasma membrane glycoproteins

  1987cited by this paper
• Immunocytochemical study of the partition and distribution of Sindbis virus glycoproteins in freeze-fractured membranes of infected baby hamster kidney cells

  1985cited by this paper
• A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei

  1976cited by this paper
• Isolation of nuclear pore complexes in association with a lamina.

  1975cited by this paper
• THE ENZYMATIC SYNTHESIS OF HOLOTRANSCARBOXYLASE FROM APOTRANSCARBOXYLASE AND (+)-BIOTIN. II. INVESTIGATION OF THE REACTION MECHANISM.

  1964cited by this paper
• AVIDIN. 1. THE USE OF (14-C)BIOTIN FOR KINETIC STUDIES AND FOR ASSAY.

  1963cited by this paper

• Bacterial ubiquitin ligase engineered for small molecule and protein target identification

  2026cites this paper
• Identifying regulators of associative learning using a protein-labelling approach in Caenorhabditis elegans

  2026cites this paper
• Subcellular localization as a driver of protein function.

  2026cites this paper
• Library‐on‐Library Intercellular Labeling for Selection of Biotin Ligase and Acceptor Peptides

  2026cites this paper
• Steric confinement-induced emission probe for monitoring protein conformations in live cells

  2026cites this paper
• Genetic Encoding of a Trifunctional Photo‐Cross‐Linker with a Cleavable Alkyl Ester Moiety

  2026cites this paper
• Deeper neuronal and glial proteomic insights using an optimized pipeline for proximity labeling proteomics

  2026cites this paper
• Attenuated lamin A-Prohibitin2 interaction leads to mitochondrial dysfunction in LMNA 289 A>G mediated Dilated Cardiomyopathy.

  2026cites this paper
• Mapping Trypanosoma Protein Interactome by Proximity-Dependent Biotinylation.

  2026cites this paper
• DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID

  2026cites this paper
• Native-state and cell type-specific proteomics using TurboID proximity labeling in mouse models.

  2026cites this paper
• Proximity labeling in neuroscience: decoding molecular landscapes for precision neurology

  2026cites this paper
• Deciphering molecular spatiotemporal information of brain cells by chemical labeling

  2026cites this paper
• A split biotin ligase approach reveals proteins associated with oligomeric alpha-synuclein during aggregation

  2026cites this paper
• MYST acetyltransferases are a targetable therapeutic vulnerability in SETBP1-mutant leukemia

  2026cites this paper
• A predicted structural interactome reveals binding interference from intrinsically disordered regions

  2026cites this paper
• Identifying Centriole-associated Factors That Induce Differentiation

  2026cites this paper
• Negative regulation of the NF-κB pathway by the ubiquitin ligase Nedd4-1(NE).

  2026cites this paper
• Refined affinity purification protocol for CAMs using minimal mouse brain material.

  2026cites this paper
• ChIP happens: from biochemical origins to the modern omics toolbox for understanding steroid hormone receptors

  2026cites this paper
• A Replication-Competent Flavivirus Genome with a Stable GFP Insertion at the NS1-NS2A Junction

  2026cites this paper
• Advances and Applications of Spatial Proteomics: From Organellar Maps to Clinical Translation

  2026cites this paper
• Establishing TurboID Proximity Labeling and mAID Conditional Knockdown System in Cryptosporidium parvum.

  2026cites this paper
• Future Research Directions on Mycobacterium tuberculosis Proteins.

  2026cites this paper
• Dexter Energy Transfer Photocatalytic Microdissection of Amyloid Plaques in Alzheimer's Disease.

  2026cites this paper
• Opportunities and challenges of proximity labeling for microbe-host cell interactions in tumor microenvironment

  2026cites this paper
• Mass spectrometry-based human spatial omics: fundamentals, innovations, and applications

  2026cites this paper
• Strawberry Notch 1 Acts as a Transcriptional Regulator Driving Oncogenic Programs in Liver Carcinogenesis.

  2026cites this paper
• Microcephaly-associated protein WDR62 supports purine metabolism by interacting with co-chaperone BAG2.

  2026cites this paper
• Photosensitizer proximity labeling captures the lipid and protein interactomes.

  2026cites this paper
• Assays for surface antigens in extracellular vesicles using proximity labeling strategy.

  2026cites this paper
• A lysine-free REC tag system for proximity-biotinylation applications

  2026cites this paper
• The mitochondrial cohibitin complex facilitates the biogenesis of inner membrane proteins

  2026cites this paper
• Decoding 'Wntch': the intertwined Wnt and Notch pathways in development and disease.

  2026cites this paper
• A brief guide to analyzing TurboID-based proximity labeling-mass spectrometry in plants

  2025cites this paper
• Multiplexed proximity labelling proteomics identifies a non-canonical Bcl-2 family interactome associated with apoptotic priming

  2025cites this paper
• Loss of UFMylation supports prostate cancer metastasis and rewires cell metabolism towards hexosamine biosynthesis

  2025cites this paper
• Sphingosine-1-phosphate signalling activates E-Syt1 to facilitate HDL-derived cholesterol transport

  2025cites this paper
• Decoding the Liver-Heart Axis in Cardiometabolic Diseases

  2025cites this paper
• Multiple FGFR1 mutations modulate tumorigenic mechanisms in glioneuronal tumors

  2025cites this paper
• Decreasing protein biotinylation background in a diatom facilitates proximity labeling of the periplastidial compartment proteome.

  2025cites this paper
• Decoding RNA–Protein Interactions: Methodological Advances and Emerging Challenges

  2025cites this paper
• The developmental factor TBX3 engages with the Wnt/β-catenin transcriptional complex in colorectal cancer to regulate metastasis genes

  2025cites this paper
• 3’UTR RNA editing driven by ADAR1 modulates MDM2 expression in breast cancer cells

  2025cites this paper
• Integrative proximal-ubiquitomics profiling for deubiquitinase substrate discovery applied to USP30.

  2025cites this paper
• Protocol for identifying components of subcellular compartments by antibody-based in situ biotinylation

  2025cites this paper
• Bacterial pathogens hijack host cell peroxisomes for replication vacuole expansion and integrity

  2025cites this paper
• Screening and identification of protein 29 of Echinococcus granulosus interacting molecules

  2025cites this paper
• In-cell proximity target validation methods for heterobifunctional molecules with CRBN- or VHL-binder using AirID

  2025cites this paper
• A series of reviews in familial cancer: genetic cancer risk in context variants of uncertain significance in MMR genes: which procedures should be followed?

  2025cites this paper
• VGLL2 and TEAD1 fusion proteins identified in human sarcoma drive YAP/TAZ-independent tumorigenesis by engaging EP300

  2025cites this paper
• In vivo proximity-labeling with miniTurboID to screen for protein-protein interactions in the filamentous ascomycete Sordaria macrospora

  2025cites this paper
• The solute carrier superfamily interactome

  2025cites this paper
• Proteomics Analysis of the TDP‐43 Interactome in Cellular Models of ALS Pathogenesis

  2025cites this paper
• Bioluminescence-activated proximity labeling for spatial multi-omics

  2025cites this paper
• A conserved role for centriolar satellites in translation of centrosomal and ciliary proteins

  2025cites this paper
• Importin-alpha transports Norrin to the nucleus to promote proliferation and Notch signaling in glioblastoma stem cells

  2025cites this paper
• Employing Expression-Matched Controls Enables High-Confidence Proximity-Based Interactome Classification

  2025influential citation
• Construction of a proximity labeling vector to identify protein-protein interactions in human stem cells

  2025cites this paper
• A Catalytically Inactive Protein Kinase C alpha Mutation Drives Chordoid Glioma by Pathway Rewiring

  2025cites this paper
• Predictable modulation of a spontaneous post-translational modification in living cells

  2025cites this paper
• Chemical biology approaches to resolve the subcellular GPCR signaling landscape

  2025cites this paper
• Proximity Labeling and SILAC-Based Proteomic Approach Identifies Proteins at the Interface of Homotypic and Heterotypic Cancer Cell Interactions

  2025cites this paper
• Chemical proteomics for a comprehensive understanding of functional activity and the interactome.

  2025cites this paper
• UBE3A ubikitina ligasaren funtzioa indargabetzen duten deubikitinasak identifikatzea eta inhibitzea Angelman sindromea tratatzeko estrategia terapeutiko gisa

  2025cites this paper
• Selective identification of epigenetic regulators at methylated genomic sites by SelectID

  2025cites this paper
• Actin cytoskeletal remodeling requires the interaction between Solo and LARG in response to substrate stiffness

  2025cites this paper
• Identification of MEF2A, MEF2C, and MEF2D interactomes in basal and Fsk-stimulated mouse MA-10 Leydig cells.

  2025cites this paper
• Overcoming Analytical Challenges in Proximity Labeling Proteomics

  2025cites this paper
• The Genetic Architecture of Congenital Diarrhea and Enteropathy

  2025cites this paper
• GPCR-targeted imaging and manipulation of homeostatic microglia in living systems

  2025cites this paper
• The mechanism of YAP/TAZ transactivation and dual targeting for cancer therapy

  2025cites this paper
• Application of mass spectrometry for the advancement of PROTACs.

  2025cites this paper
• The primate-specific Nedd4-1(NE) localizes to late endosomes in response to amino acids to suppress autophagy

  2025cites this paper
• A split ALFA tag-nanobody system for protein localization and proximity proteomics in mycobacteria

  2025cites this paper
• NEMO is essential for directing the kinases IKKα and ATM to the sites of DNA damage.

  2025cites this paper
• 1000 fold Ultra‐Photosensitized Fluorescent Protein Mimics Toward Photocatalytic Proximity Labeling and Proteomic Profiling Functions

  2025cites this paper
• Proximal partners of the organellar N-terminal acetyltransferase NAA60: insights into Golgi structure and transmembrane protein topology

  2025cites this paper
• Proximity proteomics reveals a mechanism of fatty acid transfer at lipid droplet-mitochondria- endoplasmic reticulum contact sites

  2025cites this paper
• A dual-purification system to isolate mitochondrial subpopulations

  2025cites this paper
• Expression of transgenic biotin ligases in inducible neuronal murine cell lines by integration into the mHipp11 gene locus

  2025cites this paper
• Mitochondrial genetics, signalling and stress responses

  2025cites this paper
• YibN, a bona fide interactor of the bacterial YidC insertase with effects on membrane protein insertion and membrane lipid production

  2025influential citation
• Spatiotemporally resolved mapping of extracellular proteomes via in vivo-compatible TyroID

  2025cites this paper
• Biased Signaling in G Protein-Coupled Receptors: Understanding the Biological Relevance and Tools for Probing Functionally Selective Ligands.

  2025cites this paper
• Emerging Approaches for Studying Lipid Dynamics, Metabolism, and Interactions in Cells.

  2025cites this paper
• Visible light-mediated photocatalytic coupling between tetrazoles and carboxylic acids for biomolecule labelling.

  2025cites this paper
• Armed with PRICKLE(3)s: Stabilizing WNT/PCP complexes against RNF43-mediated ubiquitination

  2025cites this paper
• Identification of E3 Ubiquitin Ligase Substrates Using Biotin Ligase-Based Proximity Labeling Approaches

  2025influential citation
• Engineered Proteins and Chemical Tools to Probe the Cell Surface Proteome

  2025cites this paper
• TMEM65 regulates and is required for NCLX-dependent mitochondrial calcium efflux

  2025cites this paper
• Characterizing host-microbe interactions with bacterial effector proteins using proximity-dependent biotin identification (BioID)

  2025cites this paper
• Investigating the Molecular Composition of Neuronal Subcompartments Using Proximity Labeling.

  2025cites this paper
• Mapping RNA-Protein Interactions via Proximity Labeling-Based Approaches.

  2025cites this paper
• Proximity labeling identification of plasma membrane eisosome proteins in Candida albicans

  2025influential citation
• BioID-Based Proximity Mapping of Transmembrane Proteins in Human Airway Cell Models.

  2025cites this paper
• PLAMseq enables the proteo-genomic characterization of chromatin-associated proteins and protein interactions in a single experimental workflow

  2025cites this paper
• USO1 Coordinates Centriolar Satellites to Regulate Male Germ Cell Proliferation and Cell Cycle Progression

  2025cites this paper
• A system for functional studies of the major virulence factor of malaria parasites

  2025cites this paper
• Bacterial ubiquitin ligase engineered for small molecule and protein target identification

  2025cites this paper