An actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides

We examined the actin-nucleating activity in polymorphonuclear leukocyte lysates prepared at various times after chemotactic peptide addition. The actin nucleation increases two- to threefold within 15 s after peptide addition, decays to basal levels within 90 s, and is largely independent of cytoplasmic calcium fluxes. The peptide-induced nucleation sites behave as free barbed ends and therefore may increase the level of polymerized actin in vivo. The new nucleation sites may also determine the cellular sites of actin polymerization. This localization of actin polymerization could be important for the directional extension of lamellipodia during chemotaxis.

• Publication year

  1986

• Venue

  Journal of Cell Biology

• Publication date

  1986-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.103.6.2707PMID 3793753PMCID 2114615
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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