Assignment of the three methionyl carbonyl carbon resonances in Streptomyces subtilisin inhibitor by a carbon-13 and nitrogen-15 double-labeling technique. A new strategy for structural studies of proteins in solution.

No abstract is available for this paper.

• Publication year

  1982

• Venue

  Biochemistry

• Publication date

  1982-11-23

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/BI00267A036PMID 7150557
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Thermal denaturation of streptomyces subtilisin inhibitor, subtilisin BPN', and the inhibitor-subtilisin complex.

  1981cited by this paper
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  1980cited by this paper
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  1980cited by this paper
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  1979cited by this paper
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  1979cited by this paper
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  1979cited by this paper
• Carbon-13 nuclear magnetic resonance: new techniques.

  1979cited by this paper
• 13C NMR studies of the binding of soybean trypsin inhibitor to trypsin.

  1979cited by this paper
• Nuclear magnetic resonance of labile protons in the basic pancreatic trypsin inhibitor.

  1979cited by this paper
• Kinetics of the exchange of individual amide protons in the basic pancreatic trypsin inhibitor.

  1979cited by this paper
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  1979cited by this paper
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  1979cited by this paper
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  1978cited by this paper
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  1977cited by this paper
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  1977cited by this paper
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  1977cited by this paper
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  1977cited by this paper
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  1976cited by this paper
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  1976cited by this paper
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  1975cited by this paper
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