miR-296 inhibits the metastasis and epithelial-mesenchymal transition of colorectal cancer by targeting S100A4

BackgroundDysregulation of microRNAs (miRNAs) is actively involved in the pathogenesis and tumorigenicity of colorectal cancer (CRC). miR-296 was found to play either oncogenic or tumor suppressive role in human cancers. However, the status of miR-296 and its function in CRC remain unknown.MethodsThe expression of miR-296 was confirmed by qRT-PCR in CRC tissues and cells, and its level was altered by corresponding miRNA vectors. Wound healing and Transwall assays were performed to detect the migration and invasion of CRC cells. The levels of proteins were measured using immunoblotting, immunohistochemistry and immunofluorescence.ResultsUnderexpression of miR-296 was disclosed in CRC tissues and cells. Its decreased level was evidently correlated with adverse clinical parameters and poor prognosis of CRC patients. In vitro experiments indicated that miR-296 inhibited CRC cell migration and invasion. Mechanically, miR-296 inhibited the epithelial-mesenchymal transition (EMT) of CRC cells. A negative correlation between miR-296 and S100A4 expression was observed in CRC tissues. Luciferase reporter assays indicated that miR-296 inversely regulated the luciferase activity of 3’-UTR of S100A4. Herein, S100A4 was found to be a downstream molecule of miR-296 in CRC. Furthermore, S100A4 mediated the anti-metastatic effects of miR-296 on EMT, migration and invasion of CRC cells.ConclusionsmiR-296 functions as an anti-metastatic factor mainly by suppressing S100A4 in CRC. It potentially acts as a prognostic predictor and a drug-target for CRC patients.

• Publication year

  2017

• Venue

  BMC Cancer

• Publication date

  2017-02-16

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1186/s12885-017-3121-zPMID 28209128PMCID 5311719
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Integrative microRNA and gene profiling data analysis reveals novel biomarkers and mechanisms for lung cancer

  2016cited by this paper
• miRNA-296-3p modulates chemosensitivity of lung cancer cells by targeting CX3CR1.

  2016cited by this paper
• Epigenetic modulation of a miR-296-5p:HMGA1 axis regulates Sox2 expression and glioblastoma stem cells

  2016cited by this paper
• Myostatin and IGF-I signaling in end-stage human heart failure: a qRT-PCR study

  2015cited by this paper
• Expression of miR-296-5p as predictive marker for radiotherapy resistance in early-stage laryngeal carcinoma

  2015cited by this paper
• Nivolumab improved survival vs dacarbazine in patients with untreated advanced melanoma

  2015cited by this paper
• Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma

  2014cited by this paper
• Decrease in blood miR-296 predicts chemotherapy resistance and poor clinical outcome in patients receiving systemic chemotherapy for metastatic colon cancer

  2013cited by this paper
• Regulatory networks defining EMT during cancer initiation and progression

  2013cited by this paper
• MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis

  2012cited by this paper
• Dysregulation of MicroRNAs in Colonic Field Carcinogenesis: Implications for Screening

  2012cited by this paper
• Cancer Statistics, 2010

  2010cited by this paper
• S100A4 and metastasis: a small actor playing many roles.

  2010cited by this paper
• Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer

  2010cited by this paper
• Colorectal cancer epidemiology: incidence, mortality, survival, and risk factors.

  2009cited by this paper
• EMT, the cytoskeleton, and cancer cell invasion

  2009cited by this paper
• MicroRNAs: Target Recognition and Regulatory Functions

  2009cited by this paper
• MicroRNAs: novel biomarkers for human cancer.

  2009cited by this paper
• Colon cancer metastasis: MACC1 and Met as metastatic pacemakers.

  2009cited by this paper
• 1070 Differential Expression of MicroRNAs in Plasma of Colorectal Cancer Patients: A Potential Marker for Colorectal Cancer Screening

  2009cited by this paper
• Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening

  2009cited by this paper
• Diagnostic and prognostic microRNAs in stage II colon cancer.

  2008cited by this paper
• microRNAs as oncogenes and tumor suppressors.

  2007cited by this paper
• MicroRNAs in tumorigenesis: a primer.

  2007cited by this paper
• S100A4, a Mediator of Metastasis*

  2006cited by this paper
• Prognostic Values of microRNAs in Colorectal Cancer

  2006cited by this paper
• Oncomirs — microRNAs with a role in cancer

  2006cited by this paper
• Epidemiology and Management of Liver Metastases From Colorectal Cancer

  2006cited by this paper
• The diverse functions of microRNAs in animal development and disease.

  2006cited by this paper
• MicroRNAs as oncogenes and tumor suppressors.

  2005cited by this paper
• The functions of animal microRNAs

  2004cited by this paper
• Prognostic significance of calcium-binding protein S100A4 in colorectal cancer.

  2002cited by this paper
• Cancer statistics

  1989cited by this paper

• MicroRNAs in Esophageal Cancer: Implications for Diagnosis, Progression, Prognosis and Chemoresistance

  2026cites this paper
• Role of S100 family proteins in colorectal cancer (CRC): an overview of their potential function as new biomarkers and therapeutic agents

  2025cites this paper
• Molecular role of miR-152-3p, miR-296-5p and miR-185-5p in urinary bladder cancer etiology

  2024cites this paper
• Silencing of KIAA1429, a N6‐methyladenine methyltransferase, inhibits the progression of colon adenocarcinoma via blocking the hypoxia‐inducible factor 1 signalling pathway

  2024cites this paper
• Hsa-miR-22-3p inhibits liver cancer cell EMT and cell migration/ invasion by indirectly regulating SPRY2

  2023cites this paper
• Unraveling the function of epithelial-mesenchymal transition (EMT) in colorectal cancer: Metastasis, therapy response, and revisiting molecular pathways.

  2023cites this paper
• MicroRNAs and colorectal cancer: clinical potential and regulatory networks

  2023cites this paper
• Novel insights on perils and promises of miRNA in understanding colon cancer metastasis and progression

  2023cites this paper
• STAT2 act a prognostic biomarker and associated with immune infiltration in kidney renal clear cell carcinoma

  2023cites this paper
• Long Noncoding XLOC_006390 Regulates the Proliferation and Metastasis of Human Colorectal Cancer via miR-296/ONECUT2 Axis

  2022cites this paper
• PYY modulates the tumorigenesis and progression of colorectal cancer unveiled by proteomics.

  2022cites this paper
• Polyploid giant cancer cells and cancer progression

  2022cites this paper
• Molecular mechanism, regulation, and therapeutic targeting of the STAT3 signaling pathway in esophageal cancer (Review)

  2022cites this paper
• miR-325-3p, a novel regulator of osteoclastogenesis in osteolysis of colorectal cancer through targeting S100A4

  2021cites this paper
• In Hepatocellular Carcinoma, miRNA-296-3p Targets MSL2 and Suppresses Cell Proliferation and Invasion

  2021cites this paper
• MicroRNA as an Important Target for Anticancer Drug Development

  2021cites this paper
• Glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1 promotes colorectal cancer cell proliferation and metastasis, and is negatively regulated by miR-296-3p

  2021cites this paper
• Exosomal circ_IFT80 Enhances Tumorigenesis and Suppresses Radiosensitivity in Colorectal Cancer by Regulating miR-296-5p/MSI1 Axis

  2021cites this paper
• HDAC3 deteriorates colorectal cancer progression via microRNA-296-3p/TGIF1/TGFβ axis

  2020cites this paper
• Molecular Mechanisms by Which S100A4 Regulates the Migration and Invasion of PGCCs With Their Daughter Cells in Human Colorectal Cancer

  2020cites this paper
• The potential of circulating exosomal RNA biomarkers in cancer

  2020cites this paper
• Involvement of MicroRNA-296 in the Inhibitory Effect of Epigallocatechin Gallate against the Migratory Properties of Anoikis-Resistant Nasopharyngeal Carcinoma Cells

  2020cites this paper
• Understanding Glioblastoma Biomarkers: Knocking a Mountain with a Hammer

  2020cites this paper
• Long Noncoding RNA CCAT1 Functions as a Competing Endogenous RNA to Upregulate ITGA9 by Sponging MiR-296-3p in Melanoma

  2020cites this paper
• MSC-derived exosomal miR-34a/c-5p and miR-29b-3p improve intestinal barrier function by targeting the Snail/Claudins signaling pathway.

  2020cites this paper
• Glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1, a potential target of miR-296, facilitates proliferation and migration of rectal cancer cells

  2020cites this paper
• Knockdown of circ_0000512 Inhibits Cell Proliferation and Promotes Apoptosis in Colorectal Cancer by Regulating miR-296-5p/RUNX1 Axis

  2020cites this paper
• Clinical significance of microRNA-125b and its contribution to ovarian carcinogenesis

  2020cites this paper
• SP1‐induced lncRNA FOXD3‐AS1 contributes to tumorigenesis of cervical cancer by modulating the miR‐296‐5p/HMGA1 pathway

  2020cites this paper
• LncRNA testis-specific transcript, Y-linked 15 (TTTY15) promotes proliferation, migration and invasion of colorectal cancer cells via regulating miR-29a-3p/DVL3 axis

  2020cites this paper
• MicroRNA-296-5p inhibits cell metastasis and invasion in nasopharyngeal carcinoma by reversing transforming growth factor-β-induced epithelial–mesenchymal transition

  2020cites this paper
• Epithelial–Mesenchymal Transition: Role in Cancer Progression and the Perspectives of Antitumor Treatment

  2020cites this paper
• Biological Implications and Clinical Potential of Metastasis-Related miRNA in Colorectal Cancer

  2020cites this paper
• Identi�cation of Key Genes and Pathways in Colorectal Cancer by Integrated Bioinformatics Analysis

  2020cites this paper
• CMAR_A_252635 4699..4714

  2020cites this paper
• OTT_A_250495 7357..7368

  2020cites this paper
• HDAC3 deteriorates colorectal cancer progression via microRNA-296-3p/TGIF1/TGFβ axis

  2020cites this paper
• MiR-199 inhibits EMT and invasion of hepatoma cells through inhibition of Snail expression.

  2019cites this paper
• MicroRNA-124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer

  2019cites this paper
• MiR-296-5p inhibits cell invasion and migration of esophageal squamous cell carcinoma by downregulating STAT3 signaling.

  2019cites this paper
• miR-296-3p targets APEX1 to suppress cell migration and invasion of non-small-cell lung cancer.

  2019cites this paper
• Tissue micro-RNAs associated with colorectal cancer prognosis: a systematic review

  2019cites this paper
• HANR promotes lymphangiogenesis of hepatocellular carcinoma via secreting miR‐296 exosome and regulating EAG1/VEGFA signaling in HDLEC cells

  2019cites this paper
• The identification of a common different gene expression signature in patients with colorectal cancer.

  2019cites this paper
• MiR-296-3p may affect the proliferation and migration of non-small cell lung cancer cells via regulating RABL3.

  2019cites this paper
• MicroRNA-296 functions as a tumor suppressor in breast cancer by targeting FGFR1 and regulating the Wnt/β-catenin signaling pathway.

  2019cites this paper
• MicroRNA-296 Targets Specificity Protein 1 to Suppress Cell Proliferation and Invasion in Cervical Cancer

  2018cites this paper
• Exosomal microRNAs as potential biomarkers for cancer cell migration and prognosis in hepatocellular carcinoma patient-derived cell models

  2018cites this paper
• MicroRNA‐296‐5p promotes healing of diabetic wound by targeting sodium‐glucose transporter 2 (SGLT2)

  2018cites this paper
• Let-7g affects cell proliferation, migration and invasion in cervical squamous cell carcinomas via targeting collagen I.

  2018cites this paper
• MicroRNA-155 acts as a tumor suppressor in colorectal cancer by targeting CTHRC1 in vitro

  2018cites this paper
• The promising role of miR-296 in human cancer.

  2018cites this paper
• miR-296-3p Negatively Regulated by Nicotine Stimulates Cytoplasmic Translocation of c-Myc via MK2 to Suppress Chemotherapy Resistance.

  2018cites this paper
• Prognostic role of snail in lung cancer

  2018cites this paper
• Depletion of S100A4+ stromal cells does not prevent HCC development but reduces the stem cell-like phenotype of the tumors

  2018cites this paper
• Prognostic value of microRNAs in colorectal cancer: a meta-analysis

  2018cites this paper
• Oncogenic and Tumor-Suppressive Roles of MicroRNAs with Special Reference to Apoptosis: Molecular Mechanisms and Therapeutic Potential

  2018cites this paper
• S100A4 Is a Biomarker and Regulator of Glioma Stem Cells That Is Critical for Mesenchymal Transition in Glioblastoma.

  2017cites this paper
• Multi-omics Approach Reveals Distinct Differences in Left- and Right-Sided Colon Cancer

  2017cites this paper
• Silencing CCT 6 A suppresses cell migration and invasion in glioblastoma in vitro

  2017cites this paper
• Molecular mechanisms and clinical implications of miRNAs in drug resistance of esophageal cancer

  2017cites this paper
• Overexpression of miR-599 is associated with metastasis in colorectal cancer via inhibition of SATB 2

  2017cites this paper
• MiR-22 inhibits lung cancer cell EMT and invasion through targeting Snail.

  2017cites this paper
• miR-155-5p modulates malignant behaviors of hepatocellular carcinoma by directly targeting CTHRC1 and indirectly regulating GSK-3β-involved Wnt/β-catenin signaling

  2017cites this paper
• Targeting epithelial-mesenchymal plasticity in cancer: clinical and preclinical advances in therapy and monitoring.

  2017cites this paper