Neutral amino acid transport in mouse peritoneal macrophages.

Neutral amino acid transport has been characterized in the mouse peritoneal macrophages elicited by thioglycolate broth. Serine, alanine, cysteine, and leucine were transported mainly in a Na+-independent manner and, within the concentration ranges tested, the transport of each amino acid was mediated by a single, saturable system. The transport of these amino acids was strongly inhibited by a wide variety of neutral amino acids, 2-aminobicyclo(2,2,1)heptane-2-carboxylic acid, a putative System L substrate, but not by glycine, proline, or 2-methylaminoisobutyrate. In this study the transport of serine has been characterized in detail. Inhibition of the transport of serine by 2-aminobicyclo(2,2,1)heptane-2-carboxylic acid or by leucine was competitive. The transport of serine which exhibited trans-stimulation was slightly stimulated by lowering the pH and was little affected by N-ethylmaleimide. It was also shown that most of the neutral amino acids were concentrated only slightly (less than 2-fold) in the macrophages, unlike in the lymphocytes and granulocytes. From these results we have concluded that in the macrophages serine, cysteine, alanine, leucine, and presumably many other neutral amino acids are transported almost exclusively through a common transport system analogous to System L.

• Publication year

  1987

• Venue

  Journal of Biological Chemistry

• Publication date

  1987-09-25

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)45159-9PMID 3115975
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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