Phospholipid‐assisted protein folding: phosphatidylethanolamine is required at a late step of the conformational maturation of the polytopic membrane protein lactose permease

No abstract is available for this paper.

• Publication year

  1998

• Venue

  EMBO Journal

• Publication date

  1998-09-15

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1093/emboj/17.18.5255PMID 9736605PMCID PMC1170853
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Steric chaperones.

  1998cited by this paper
• Rapid Transmembrane Movement of Newly Synthesized Phosphatidylethanolamine across the Inner Membrane of Escherichia coli *

  1998cited by this paper
• Binding of monoclonal antibody 4B1 to homologs of the lactose permease of Escherichia coli

  1997cited by this paper
• Helix packing in polytopic membrane proteins: the lactose permease of Escherichia coli.

  1997cited by this paper
• HtrA Heat Shock Protease Interacts with Phospholipid Membranes and Undergoes Conformational Changes*

  1997cited by this paper
• Do molecular chaperones have to be proteins?

  1997cited by this paper
• The last two cytoplasmic loops in the lactose permease of Escherichia coli comprise a discontinuous epitope for a monoclonal antibody.

  1997influential reference
• In vitro membrane integration of leader peptidase depends on the Sec machinery and anionic phospholipids and can occur post‐translationally

  1997cited by this paper
• Cotranslational Protein Folding*

  1997cited by this paper
• Do molecular chaperones have to be proteins

  1997cited by this paper
• Molecular basis for membrane phospholipid diversity: why are there so many lipids?

  1997cited by this paper
• Molecular biology of the lactose carrier of Escherichia coli.

  1996cited by this paper
• Monoclonal antibody 4B1 alters the pKa of a carboxylic acid at position 325 (helix X) of the lactose permease of Escherichia coli.

  1996cited by this paper
• Molecular chaperones in cellular protein folding

  1996cited by this paper
• Identification of the epitope for monoclonal antibody 4B1 which uncouples lactose and proton translocation in the lactose permease of Escherichia coli.

  1996cited by this paper
• Chaperonin-promoted Post-translational Membrane Insertion of a Multispanning Membrane Protein Lactose Permease*

  1996influential reference
• Protein folding in the cell: competing models of chaperonin function

  1996cited by this paper
• Lipopolysaccharides and divalent cations are involved in the formation of an assembly‐competent intermediate of outer‐membrane protein PhoE of E.coli.

  1996cited by this paper
• A Phospholipid Acts as a Chaperone in Assembly of a Membrane Transport Protein (*)

  1996influential reference
• The role of molecular chaperones in protein folding

  1995cited by this paper
• Phosphatidylethanolamine Is Required for in Vivo Function of the Membrane-associated Lactose Permease of Escherichia coli(*)

  1995influential reference
• Generation of a stable folding intermediate which can be rescued by the chaperonins GroEL and GroES

  1994cited by this paper
• Chaperonins as potential gene regulatory factors. In vitro interaction and solubilization of NifA, the nif transcriptional activator, with GroEL.

  1994cited by this paper
• Role of the charge pair aspartic acid-237-lysine-358 in the lactose permease of Escherichia coli.

  1993cited by this paper
• Bacterial solute transport proteins in their lipid environment.

  1993cited by this paper
• Molecular chaperone functions of heat-shock proteins.

  1993cited by this paper
• A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport.

  1993cited by this paper
• Role of the major heat shock proteins as molecular chaperones.

  1993cited by this paper
• Chapter 2 The nature of the lipid-protein interface and the influence of protein structure on protein-lipid interactions

  1993cited by this paper
• Micelle-assisted protein folding. Denatured rhodanese binding to cardiolipin-containing lauryl maltoside micelles results in slower refolding kinetics but greater enzyme reactivation.

  1992cited by this paper
• Folding on the ribosome of Escherichia coli tryptophan synthase beta subunit nascent chains probed with a conformation-dependent monoclonal antibody.

  1992cited by this paper
• Use of in vitro protein synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies.

  1991influential reference
• Investigating protein conformation, dynamics and folding with monoclonal antibodies.

  1991cited by this paper
• Sequence and inactivation of the pss gene of Escherichia coli. Phosphatidylethanolamine may not be essential for cell viability.

  1991cited by this paper
• Sequence and inactivation of the pss gene of Escherichia coli. Phosphatidylethanolamine may not be essential for cell viability.

  1991influential reference
• In vivo expression of the lacY gene in two segments leads to functional lac permease.

  1990cited by this paper
• Biosynthesis and function of phospholipids in Escherichia coli.

  1990cited by this paper
• In vitro membrane assembly of a polytopic, transmembrane protein results in an enzymatically active conformation

  1989influential reference
• Characterization and functional reconstitution of a soluble form of the hydrophobic membrane protein lac permease from Escherichia coli.

  1989influential reference
• A five-residue sequence near the carboxyl terminus of the polytopic membrane protein lac permease is required for stability within the membrane.

  1989cited by this paper
• In vitro insertion of leader peptidase into Escherichia coli membrane vesicles

  1988cited by this paper
• The effects of pH on proton sugar symport activity of the lactose permease purified from Escherichia coli.

  1988influential reference
• The N-terminal region of Escherichia coli lactose permease mediates membrane contact of the nascent polypeptide chain.

  1987cited by this paper
• Substrate-induced membrane association of phosphatidylserine synthase from Escherichia coli

  1986cited by this paper
• Active transport in Escherichia coli: passage to permease.

  1986cited by this paper
• Active transport in Escherichia coli: passage to permease.

  1986cited by this paper
• Alkaline phosphatase and OmpA protein can be translocated posttranslationally into membrane vesicles of Escherichia coli

  1985cited by this paper
• A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

  1985cited by this paper
• Functional and immunochemical characterization of a mutant of Escherichia coli energy uncoupled for lactose transport.

  1985cited by this paper
• Escherichia coli membrane vesicles with elevated phosphatidic acid levels. A detergent-free system for in vitro phospholipid synthesis.

  1984influential reference
• In vitro translocation of bacterial proteins across the plasma membrane of Escherichia coli.

  1984cited by this paper
• The phospholipid requirement for activity of the lactose carrier of Escherichia coli.

  1984cited by this paper
• Unidimensional thin-layer chromatography of phospholipids on boric acid-impregnated plates.

  1982cited by this paper
• Preparation, characterization, and properties of monoclonal antibodies against the lac carrier protein from Escherichia coli.

  1982cited by this paper
• Cloning of genes involved in membrane lipid synthesis. Effects of amplification of phosphatidylserine synthase in Escherichia coli.

  1981influential reference
• Purification and reconstitution of functional lactose carrier from Escherichia coli.

  1981cited by this paper
• Intracellular Distribution of Enzymes of Phospholipid Metabolism in Several Gram-Negative Bacteria

  1977cited by this paper
• Studies on the metabolism of ATP by isolated bacterial membranes: formation and metabolism of membrane-bound phosphatidic acid.

  1971influential reference
• Determination of lipid phosphorus in the nanomolar range.

  1970cited by this paper

• Micron-scale, liquid-liquid phase separation in ternary lipid membranes containing DPPE

  2025cites this paper
• Multi-omics identify hallmark protein and lipid features of small extracellular vesicles circulating in human plasma

  2025cites this paper
• Preparation of Uniformly Oriented Inverted Inner (Cytoplasmic) Membrane Vesicles from Gram-Negative Bacterial Cells.

  2024cites this paper
• The solute carrier SPNS2 recruits PI(4,5)P2 to synergistically regulate transport of sphingosine-1-phosphate

  2023cites this paper
• Molecular Dynamics Simulation of Antimicrobial Peptide CM15 in Staphylococcus Aureus and Escherichia coli Model Bilayer Lipid

  2023cites this paper
• Biology and roles in diseases of selenoprotein I (SELENOI) characterized by ethanolamine phosphotransferase activity and antioxidant potential.

  2023cites this paper
• Interface analysis of DnaK-GrpE-DnaJ chaperon system of Mycobacterium tuberculosis

  2022cites this paper
• Cell-Free Synthesis Strategies to Probe Co-translational Folding of Proteins Within Lipid Membranes.

  2022cites this paper
• Insights into the structure and function of the human organic anion transporter 1 in lipid bilayer membranes

  2022cites this paper
• Lipidomic and Membrane Mechanical Signatures in Triple-Negative Breast Cancer: Scope for Membrane-Based Theranostics

  2022cites this paper
• Methods to study folding of alpha-helical membrane proteins in lipids

  2022cites this paper
• Beyond Proteostasis: Lipid Metabolism as a New Player in ER Homeostasis

  2021cites this paper
• Phospholipid Asymmetry in Biological Membranes: Is the Role of Phosphatidylethanolamine Underappreciated?

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• Structures and General Transport Mechanisms by the Major Facilitator Superfamily (MFS)

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  2021cites this paper
• The Implications for Cells of the Lipid Switches Driven by Protein–Membrane Interactions and the Development of Membrane Lipid Therapy

  2020cites this paper
• Membranes as the third genetic code

  2020cites this paper
• Cell-free expression tools to study co-translational folding of alpha helical membrane transporters

  2020cites this paper
• Regulated Alternative Translocation: A Mechanism Regulating Transmembrane Proteins Through Topological Inversion.

  2020cites this paper
• Lipid composition of the cancer cell membrane

  2020cites this paper
• FgPsd2, a phosphatidylserine decarboxylase of Fusarium graminearum, regulates development and virulence.

  2020cites this paper
• The lipid-dependent structure and function of LacY can be recapitulated and analyzed in phospholipid-containing detergent micelles

  2019cites this paper
• The meningococcal vaccine antigen GNA2091 is an analogue of YraP and plays key roles in outer membrane stability and virulence

  2019cites this paper
• Membrane curvature affects the stability and folding kinetics of bacteriorhodopsin

  2019cites this paper
• Influence of cholesterol on human calcitonin channel formation. Possible role of sterol as molecular chaperone

  2019cites this paper
• Lipid-Assisted Membrane Protein Folding and Topogenesis

  2019cites this paper
• Structural and functional characterization of protein–lipid interactions of the Salmonella typhimurium melibiose transporter MelB

  2018cites this paper
• Biophysical Analysis of Sec-Mediated Protein Translocation in Nanodiscs

  2018cites this paper
• Amphipol-Assisted Cell-Free Expression of Membrane Proteins

  2018cites this paper
• Liposome-chaperoned cell-free synthesis for the design of proteoliposomes: Implications for therapeutic delivery.

  2018cites this paper
• Role of cardiolipin in stability of integral membrane proteins.

  2017cites this paper
• Interaction of phospholipase C with liposome: A conformation transition of the enzyme is critical and specific to liposome composition for burst hydrolysis and fusion in concert.

  2017cites this paper
• Phosphatidylserine synthase 2 and phosphatidylserine decarboxylase are essential for aminophospholipid synthesis in T rypanosoma brucei

  2017cites this paper
• Mammalian Glucose Transporter Activity Is Dependent upon Anionic and Conical Phospholipids*

  2016cites this paper
• The plasma membrane as a capacitor for energy and metabolism.

  2016cites this paper
• Highly Hydrophilic Segments Attached to Hydrophobic Peptides Translocate Rapidly across Membranes.

  2016cites this paper
• Modulation of Human and Malarial Glucose Transporter Activity by Lipids and Small Molecules

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• The Impacts of ProP Putative Interactors on the Localization and Activity of the Transporter ProP, and on Escherichia coli Phenotype

  2016cites this paper
• Lateral Diffusion and Molecular Interaction in a Bilayer Membrane Consisting of Partially Fluorinated Phospholipids.

  2016cites this paper
• Dynamic Lipid-dependent Modulation of Protein Topology by Post-translational Phosphorylation*

  2016cites this paper
• Phosphatidylethanolamine Metabolism in Health and Disease.

  2016cites this paper
• Protein–Lipid Interactions in the Brain

  2015cites this paper
• Anionic Lipids Modulate the Activity of the Aquaglyceroporin GlpF.

  2015cites this paper
• Transport of Phosphatidylserine from the Endoplasmic Reticulum to the Site of Phosphatidylserine Decarboxylase2 in Yeast

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• Unspecific membrane protein–lipid recognition: combination of AFM imaging, force spectroscopy, DSC and FRET measurements

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• Structural insights into functional lipid-protein interactions in secondary transporters.

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• The Sec translocon mediated protein transport in prokaryotes and eukaryotes

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• Folding membrane proteins in vitro: a table and some comments.

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• Application of novel methods using synthetic biology tools to investigate solvent toxicity in bacteria

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• Membrane protein synthesis in cell‐free systems: From bio‐mimetic systems to bio‐membranes

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• Rescue of easily shocked mutant seizure sensitivity in drosophila adults

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• Subcellular Localization and Logistics of Integral Membrane Protein Biogenesis in Escherichia coli

  2013cites this paper
• The ins and outs of phosphatidylethanolamine synthesis in Trypanosoma brucei.

  2013cites this paper
• Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers

  2013cites this paper
• A retrospective: use of Escherichia coli as a vehicle to study phospholipid synthesis and function.

  2013cites this paper
• Phospholipid-lactose permease interaction as reported by a head-labeled pyrene phosphatidylethanolamine: a FRET study.

  2013cites this paper
• Proper Fatty Acid Composition Rather than an Ionizable Lipid Amine Is Required for Full Transport Function of Lactose Permease from Escherichia coli*

  2013cites this paper
• Lipid dependencies, biogenesis and cytoplasmic micellar forms of integral membrane sugar transport proteins of the bacterial phosphotransferase system.

  2013cites this paper
• Lateral Membrane Heterogeneity Probed by FRET Spectroscopy and Microscopy

  2012cites this paper
• Molecular genetic and biochemical approaches for defining lipid-dependent membrane protein folding.

  2012cites this paper
• Progress in understanding the role of lipids in membrane protein folding.

  2012cites this paper
• The CpxAR Two-component System Regulates a Complex Envelope Stress Response in Gram-negative Bacteria

  2012cites this paper
• Lipid-dependent Generation of Dual Topology for a Membrane Protein*

  2012influential citation
• Phase Behavior Of Cardiolipin

  2012cites this paper
• Phosphatidylethanolamine and Cardiolipin Differentially Affect the Stability of Mitochondrial Respiratory Chain Supercomplexes

  2012cites this paper
• 3.13 The Membrane Factor: Biophysical Studies of Alpha Helical Transmembrane Protein Folding

  2012cites this paper
• Advanced FRET methodologies: protein-lipid selectivity detection and quantification.

  2012cites this paper
• The Cell-Free Integration of a Polytopic Mitochondrial Membrane Protein into Liposomes Occurs Cotranslationally and in a Lipid-Dependent Manner

  2012cites this paper
• Specificity of intramembrane protein-lipid interactions.

  2011cites this paper
• Schwille Lipid Polymorphisms and Membrane Shape Zimmerberg

  2011cites this paper
• Lipid-protein interactions as determinants of membrane protein structure and function.

  2011cites this paper
• Assembly of bacterial inner membrane proteins.

  2011cites this paper
• High isoelectric point sub-proteome analysis of Acinetobacter radioresistens S13 reveals envelope stress responses induced by aromatic compounds.

  2011cites this paper
• Cardiolipin deficiency in Rhodobacter sphaeroides alters the lipid profile of membranes and of crystallized cytochrome oxidase, but structure and function are maintained.

  2011cites this paper
• FMP30 is required for the maintenance of a normal cardiolipin level and mitochondrial morphology in the absence of mitochondrial phosphatidylethanolamine synthesis

  2011cites this paper
• Plasticity of lipid-protein interactions in the function and topogenesis of the membrane protein lactose permease from Escherichia coli

  2010cites this paper
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  2010cites this paper
• Cell-free synthesis and folding of transmembrane OmpA reveals higher order structures and premature truncations.

  2010cites this paper
• Identification of Specific Lipid-binding Sites in Integral Membrane Proteins*

  2010cites this paper
• Lactose permease lipid selectivity using Förster resonance energy transfer.

  2010cites this paper
• Mitochondrial membrane biogenesis: phospholipids and proteins go hand in hand

  2009cites this paper
• Calcium-induced formation of subdomains in phosphatidylethanolamine-phosphatidylglycerol bilayers: a combined DSC, 31P NMR, and AFM study.

  2009cites this paper
• Lipid-dependent membrane protein topogenesis.

  2009influential citation
• Enzymology and Molecular Genetics of Biosynthetic Enzymes for (1,3)-β-Glucans

  2009cites this paper
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  2007cites this paper
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  2007cites this paper
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  2007cites this paper
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