Antibody plaque-forming cells: kinetics of primary and secondary responses.

Numbers of Jerne plaque-forming cells per spleen, serum hemolysin concentrations, and the degree of resistance of the serum hemolysin to degradation by 2-mercaptoethanol have been determined in adult CBA and C3D2F1 mice as functions of time following primary or secondary intravenous sensitization with 4 × 108 sheep erythrocytes. The characteristic secondary response yields fewer PFC and lower serum antibody concentrations than the primary response. When an adequate time interval (9 weeks or more) elapses between primary and secondary antigen injections, the magnitude of the secondary response begins to approach that of the primary. Primary serum hemolysin is mercaptoethanol sensitive (19S immunoglobulin) while secondary serum hemolysin is largely mercaptoethanol resistant (7S immunoglobulin). Generation times of 7 and 9 hr are obtained for plaque-forming cells during the first 4 days of the primary response. A mathematical model is presented which relates numbers of antibody-forming cells to serum hemolysin concentrations. Using the model together with available experimental data, it is concluded that the great majority of the cells producing 7S hemolysin probably are not detected by the Jerne technique, as applied to the spleen.

• Publication year

  1966

• Venue

  Journal of Immunology

• Publication date

  1966-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.96.4.559PMID 5936285
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Generation times of 7 and 9 hours were obtained for plaque-forming cells during the first 4 days of the primary response.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• Primary serum hemolysin is 2-mercaptoethanol sensitive and corresponds to 19S immunoglobulin, whereas secondary serum hemolysin is largely 2-mercaptoethanol resistant and corresponds to 7S immunoglobulin.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• With an interval of 9 weeks or more between antigen injections, the secondary response begins to approach the magnitude of the primary response.

  Confidence 0.92

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• The secondary response produces fewer Jerne plaque-forming cells and lower serum hemolysin concentrations than the primary response.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review

• 19s immunoglobulin

  immunoglobulin

  The sedimentation class associated here with the mercaptoethanol-sensitive serum hemolysin fraction.

  Aliases: 19S antibody

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• 2-mercaptoethanol

  chemical

  A reducing reagent used to test whether serum hemolysin activity is sensitive to reduction.

  Aliases: mercaptoethanol, ME

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• 7s immunoglobulin

  immunoglobulin

  The sedimentation class associated here with the mercaptoethanol-resistant serum hemolysin fraction.

  Aliases: 7S antibody

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• generation time

  kinetic parameter

  The estimated doubling-time interval for plaque-forming cells during the early primary response.

  Aliases: cell generation time

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• jerne plaque-forming cells

  cell type

  Antibody-forming spleen cells counted by the Jerne plaque assay in the sensitized mice.

  Aliases: PFC, plaque-forming cells

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• primary response

  immune response

  The immune response measured after the first intravenous sensitization with sheep erythrocytes.

  Aliases: primary antibody response

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• secondary response

  immune response

  The immune response measured after a subsequent intravenous sensitization with sheep erythrocytes.

  Aliases: secondary antibody response

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• serum hemolysin

  antibody

  Hemolytic antibody activity measured in the serum after sensitization with sheep erythrocytes.

  Aliases: serum antibody, hemolysin

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review

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