Biochemical Characterization of a Human Band 4.1-related Protein-tyrosine Phosphatase, PTPH1 (*)

PTPH1 is a human protein-tyrosine phosphatase with homology to the band 4.1 superfamily of cytoskeleton-associated proteins. Here, we report the purification and biochemical characterization of this enzyme from baculovirus-infected insect cells. The purified protein exhibited an apparent Mr of 120,000 on SDS gels. The native enzyme dephosphorylated both myelin basic protein (MBP) and reduced, carboxamidomethylated, and maleylated lysozyme (RCML) but was over 5-fold more active on MBP. The K values for the two substrates were similar (1.45 μM for MBP and 1.6 μM for RCML). Phosphorylation of PTPH1 by protein kinase C in vitro resulted in a decrease in K but had no effect on Vmax. Removal of the NH2-terminal band 4.1 homology domain of PTPH1 by limited trypsin cleavage stimulated dephosphorylation of RCML but inhibited its activity toward MBP. The dephosphorylation of RCML by full-length PTPH1 was enhanced up to 6-fold by unphosphorylated MBP and increasing ionic strength up to 0.2 M NaCl, whereas trypsinized preparations of PTPH1 containing the isolated catalytic domain were unaffected. These results suggest that in addition to a potential role in controlling subcellular localization, the NH2-terminal band 4.1 homology domain of PTPH1 may exert a direct effect on catalytic function.

• Publication year

  1995

• Venue

  Journal of Biological Chemistry

• Publication date

  1995-08-25

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/JBC.270.34.20067PMID 7544351
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• DHR domains in syntrophins, neuronal NO synthases and other intracellular proteins.

  1995cited by this paper
• Enhanced expression of multiple protein tyrosine phosphatases in the regenerating mouse liver: isolation of PTP-RL10, a novel cytoplasmic-type phosphatase with sequence homology to cytoskeletal protein 4.1.

  1995cited by this paper
• ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons

  1994cited by this paper
• Purification and characterization of PTP2C, a widely distributed protein tyrosine phosphatase containing two SH2 domains.

  1994cited by this paper
• atpk1, a novel ribosomal protein kinase gene from Arabidopsis. II. Functional and biochemical analysis of the encoded protein.

  1994cited by this paper
• Identification of a novel protein tyrosine phosphatase with sequence homology to the cytoskeletal proteins of the band 4.1 family

  1994cited by this paper
• A novel protein-tyrosine phosphatase with homology to both the cytoskeletal proteins of the band 4.1 family and junction-associated guanylate kinases.

  1994cited by this paper
• Protein-tyrosine phosphatases.

  1994cited by this paper
• Molecular cloning of a novel protein‐tyrosine phosphatase containing a membrane‐binding domain and GLGF repeats

  1994cited by this paper
• Cloning and characterization of PTPL1, a protein tyrosine phosphatase with similarities to cytoskeletal-associated proteins.

  1994cited by this paper
• Cloning and characterization of hdlg: the human homologue of the Drosophila discs large tumor suppressor binds to protein 4.1.

  1994cited by this paper
• Src kinase associates with a member of a distinct subfamily of protein-tyrosine phosphatases containing an ezrin-like domain.

  1994cited by this paper
• Characterization of two structurally related Xenopus laevis protein tyrosine phosphatases with homology to lipid-binding proteins.

  1994cited by this paper
• Membrane-actin microfilament connections: an increasing diversity of players related to band 4.1.

  1994cited by this paper
• Ezrin has properties to self-associate at the plasma membrane.

  1994cited by this paper
• In vitro binding studies suggest a membrane-associated complex between erythroid p55, protein 4.1, and glycophorin C.

  1994cited by this paper
• Multi‐site phosphorylation of the protein tyrosine phosphatase, PTP1B: identification of cell cycle regulated and phorbol ester stimulated sites of phosphorylation.

  1993cited by this paper
• MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo.

  1993cited by this paper
• Introduction: protein tyrosine phosphatases.

  1993cited by this paper
• Protein tyrosine phosphatases.

  1993cited by this paper
• The ezrin-like family of tyrosine kinase substrates: receptor-specific pattern of tyrosine phosphorylation and relationship to malignant transformation.

  1993cited by this paper
• Focal adhesion kinase: an integrin-linked protein tyrosine kinase.

  1993cited by this paper
• Heterotypic and homotypic associations between ezrin and moesin, two putative membrane-cytoskeletal linking proteins.

  1993cited by this paper
• Purification and characterization of a protein tyrosine phosphatase containing SH2 domains.

  1993cited by this paper
• 1002 protein phosphatases?

  1992cited by this paper
• Purification and characterization of a human recombinant T-cell protein-tyrosine-phosphatase from a baculovirus expression system.

  1991cited by this paper
• Cytoskeletal targets for oncogenic tyrosine kinases.

  1991cited by this paper
• Identification, cloning, and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to cytoskeletal protein 4.1.

  1991cited by this paper
• Phosphorylation of protein 4.1 on tyrosine-418 modulates its function in vitro.

  1991cited by this paper
• The discs-large tumor suppressor gene of Drosophila encodes a guanylate kinase homolog localized at septate junctions.

  1991cited by this paper
• Protein kinases. Regulation by autoinhibitory domains.

  1990cited by this paper
• Identification of an autoinhibitory domain in calcineurin.

  1990cited by this paper
• cDNA cloning and sequencing of the protein‐tyrosine kinase substrate, ezrin, reveals homology to band 4.1.

  1989cited by this paper
• Rapid phosphorylation and reorganization of ezrin and spectrin accompany morphological changes induced in A-431 cells by epidermal growth factor

  1989cited by this paper

• Interactions of the protein tyrosine phosphatase PTPN3 with viral and cellular partners through its PDZ domain: insights into structural determinants and phosphatase activity

  2023influential citation
• Signaling mechanisms in red blood cells: A view through the protein phosphorylation and deformability

  2023influential citation
• Association of SNPs within PTPN3 gene with wool production and growth traits in a dual-purpose sheep population

  2022cites this paper
• The Protein Tyrosine Phosphatase H1 PTPH1 Supports Proliferation of Keratinocytes and is a Target of the Human Papillomavirus Type 8 E6 Oncogene

  2019cites this paper
• Characterization Of Pth Type-2 Receptor (pth2rb) And Its Endogenous Ligand Pth2 In Zebrafish Development

  2019cites this paper
• Etude structurale et fonctionnelle de la phosphatase humaine PTPN4

  2014cites this paper
• PTP-PEST.protein tyrosine phosphatase Identification of p130(cas) as a substrate for the

  2013cites this paper
• Altered phosphorylation of cytoskeleton proteins in sickle red blood cells: the role of protein kinase C, Rac GTPases, and reactive oxygen species.

  2010cites this paper
• Towards understanding the role of the ubiquitinligase E6AP in human disease

  2009cites this paper
• The protein tyrosine phosphatase PTPN4/PTP-MEG1, an enzyme capable of dephosphorylating the TCR ITAMs and regulating NF-kappaB, is dispensable for T cell development and/or T cell effector functions.

  2008cites this paper
• Suppression of hepatitis B viral gene expression by protein-tyrosine phosphatase PTPN3.

  2007cites this paper
• Protein tyrosine phosphatase H1 is a target of the E6 oncoprotein of high-risk genital human papillomaviruses.

  2007cites this paper
• PTPN3 and PTPN4 tyrosine phosphatase expression in human gastric adenocarcinoma.

  2006cites this paper
• PTPH1 Is a Predominant Protein-tyrosine Phosphatase Capable of Interacting with and Dephosphorylating the T Cell Receptor ζ Subunit*

  2004cites this paper
• Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1232 Protein Tyrosine Phosphatases as Regulators of Receptor Tyrosine Kinases

  2003cites this paper
• Protein tyrosine phosphatases.

  2002cites this paper
• Translocation of protein tyrosine phosphatase Pez/PTPD2/PTP36 to the nucleus is associated with induction of cell proliferation.

  2000cites this paper
• Tyrosine phosphorylation of p97 regulates transitional endoplasmic reticulum assembly in vitro.

  2000cites this paper
• N-(cyclohexanecarboxyl)-O-phospho-l-serine, a minimal substrate for the dual-specificity protein phosphatase IphP.

  2000cites this paper
• An Acth-Activated Protein Tyrosine Phosphatase (PTP) is Modulated by Pka-Mediated Phosphorylation

  2000cites this paper
• The Role of Protein Phosphatases in Cell Signaling by the High-Affinity Receptor for Immunoglobulin E

  1999cites this paper
• MIR Is a Novel ERM-like Protein That Interacts with Myosin Regulatory Light Chain and Inhibits Neurite Outgrowth*

  1999cites this paper
• Small Phosphomonoesters as Probes of Protein-Tyrosine Phosphatase Active Sites

  1999influential citation
• Identification of the Cell Cycle Regulator VCP (p97/CDC48) as a Substrate of the Band 4.1-related Protein-tyrosine Phosphatase PTPH1*

  1999cites this paper
• Regulation of Phosphorylation Level and Distribution of PTP36, a Putative Protein Tyrosine Phosphatase, by Cell-Substrate Adhesion*

  1999cites this paper
• Band 3-Mediated Flip-Flop and Phosphatase-Catalyzed Cleavage of a Long-Chain Alkyl Phosphate Anion in the Human Erythrocyte Membrane

  1998cites this paper
• Lymphocyte activation: the coming of the protein tyrosine phosphatases.

  1998cites this paper
• P-TEN, the tumor suppressor from human chromosome 10q23, is a dual-specificity phosphatase.

  1997cites this paper
• PTEN: sometimes taking it off can be better than putting it on.

  1997cites this paper
• INVITED EDITORIAL PTEN:Sometimes Taking ItOffCanBeBetter thanPutting ItOn

  1997cites this paper
• Serine Phosphorylation-dependent Association of the Band 4.1-related Protein-tyrosine Phosphatase PTPH1 with 14-3-3β Protein*

  1997cites this paper
• N K Tonks PTP-PEST . cytosolic protein tyrosine phosphatase Identification of p 130 ( cas ) as a substrate for the

  1996cites this paper
• Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST

  1996cites this paper
• Structure and function of the protein tyrosine phosphatases.

  1996cites this paper
• The Properties of the Protein Tyrosine Phosphatase PTPMEG*

  1996cites this paper
• Structure and function of theprotein tyrosine phosphatases

  1996cites this paper