Serine Phosphorylation-dependent Association of the Band 4.1-related Protein-tyrosine Phosphatase PTPH1 with 14-3-3β Protein*

PTPH1 is a human protein-tyrosine phosphatase with homology to the band 4.1 superfamily of cytoskeletal-associated proteins. PTPH1 was found to associate with 14-3-3β using a yeast two-hybrid screen, and its interaction could be reconstituted in vitro using recombinant proteins. Examination of the interaction between 14-3-3β and various deletion mutants of PTPH1 by two-hybrid tests suggested that the integrity of the PTP is important for this binding. Although both PTPH1 and Raf-1 form complexes with 14-3-3β, they appear to do so independently. Binding of 14-3-3β to PTPH1in vitro was abolished by pretreating PTPH1 with potato acid phosphatase and was greatly enhanced by pretreating with Cdc25C-associated protein kinase. Thus the association between PTPH1 and 14-3-3β is phosphorylation-dependent. Two novel motifs RSLS359VE and RVDS853EP in PTPH1 were identified as major 14-3-3β-binding sites, both of which are distinct from the consensus binding motif RSXSXP recently found in Raf-1. Mutation of Ser359 and Ser853 to alanine significantly reduced the association between 14-3-3β and PTPH1. Furthermore, association of PTPH1 and 14-3-3β was detected in several cell lines and was regulated in response to extracellular signals. These results raise the possibility that 14-3-3β may function as an adaptor molecule in the regulation of PTPH1 and may provide a link between serine/threonine and tyrosine phosphorylation-dependent signaling pathways.

• Publication year

  1997

• Venue

  Journal of Biological Chemistry

• Publication date

  1997-10-24

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/jbc.272.43.27281PMID 9341175
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Serine Phosphorylation of Cbl Induced by Phorbol Ester Enhances Its Association with 14-3-3 Proteins in T Cells via a Novel Serine-rich 14-3-3-binding Motif*

  1997cited by this paper
• P-TEN, the tumor suppressor from human chromosome 10q23, is a dual-specificity phosphatase.

  1997cited by this paper
• 14-3-3 and its possible role in co-ordinating multiple signalling pathways.

  1996cited by this paper
• Serine phosphorylation of death agonist BAD in response to survival factor results in binding to 14-3-3 not BCL-X(L)

  1996cited by this paper
• Olfactory learning deficits in mutants for leonardo, a Drosophila gene encoding a 14-3-3 protein.

  1996cited by this paper
• From form to function: signaling by protein tyrosine phosphatases.

  1996cited by this paper
• 14-3-3 proteins associate with phosphorylated simple epithelial keratins during cell cycle progression and act as a solubility cofactor

  1996cited by this paper
• Molecular Glue: Kinase Anchoring Minireview

  1996cited by this paper
• Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine.

  1996cited by this paper
• Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family.

  1996cited by this paper
• Identification of a Binding Sequence for the 14-3-3 Protein within the Cytoplasmic Domain of the Adhesion Receptor, Platelet Glycoprotein Ib (*)

  1996cited by this paper
• Activation-modulated Association of 14–3–3 Proteins with Cbl in T Cells*

  1996cited by this paper
• Molecular glue: kinase anchoring and scaffold proteins.

  1996cited by this paper
• 14-3-3 is not essential for Raf-1 function: identification of Raf-1 proteins that are biologically activated in a 14-3-3- and Ras-independent manner

  1995cited by this paper
• 14-3-3 proteins associate with cdc25 phosphatases.

  1995cited by this paper
• Bcr and Raf form a complex in vivo via 14‐3‐3 proteins.

  1995cited by this paper
• 14-3-3 Proteins: Hot numbers in signal transduction

  1995cited by this paper
• Identification of the Site of Interaction of the 14-3-3 Protein with Phosphorylated Tryptophan Hydroxylase (*)

  1995cited by this paper
• Inhibition of phosphatidylinositol 3-kinase activity by association with 14-3-3 proteins in T cells.

  1995cited by this paper
• Enhanced expression of multiple protein tyrosine phosphatases in the regenerating mouse liver: isolation of PTP-RL10, a novel cytoplasmic-type phosphatase with sequence homology to cytoskeletal protein 4.1.

  1995cited by this paper
• Origin of PDZ (DHR, GLGF) domains.

  1995cited by this paper
• Regulation of Raf‐1 kinase activity by the 14‐3‐3 family of proteins.

  1995cited by this paper
• Pez: a novel human cDNA encoding protein tyrosine phosphatase- and ezrin-like domains.

  1995cited by this paper
• FAP-1: a protein tyrosine phosphatase that associates with Fas.

  1995cited by this paper
• Biochemical Characterization of a Human Band 4.1-related Protein-tyrosine Phosphatase, PTPH1 (*)

  1995cited by this paper
• Structure of a 14-3-3 protein and implications for coordination of multiple signalling pathways

  1995cited by this paper
• Crystal structure of the zeta isoform of the 14-3-3 protein

  1995cited by this paper
• Isoforms of 14‐3‐3 protein can form homo‐ and heterodimers in vivo and in vitro: implications for function as adapter proteins

  1995cited by this paper
• Expression of PTPH1, a rat protein tyrosine phosphatase, is restricted to the derivatives of a specific diencephalic segment.

  1995cited by this paper
• A novel protein-tyrosine phosphatase with homology to both the cytoskeletal proteins of the band 4.1 family and junction-associated guanylate kinases.

  1994cited by this paper
• Association of the protein kinases c-Bcr and Bcr-Abl with proteins of the 14-3-3 family.

  1994cited by this paper
• Activation of Raf-1 by 14-3-3 proteins

  1994cited by this paper
• Stimulatory effects of yeast and mammalian 14-3-3 proteins on the Raf protein kinase.

  1994cited by this paper
• atpk1, a novel ribosomal protein kinase gene from Arabidopsis. II. Functional and biochemical analysis of the encoded protein.

  1994cited by this paper
• Purification of a serine kinase that associates with and phosphorylates human Cdc25C on serine 216.

  1994cited by this paper
• Cloning and characterization of PTPL1, a protein tyrosine phosphatase with similarities to cytoskeletal-associated proteins.

  1994cited by this paper
• cDNA cloning of a novel protein tyrosine phosphatase with homology to cytoskeletal protein 4.1 and its expression in T-lineage cells.

  1994cited by this paper
• Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation.

  1994cited by this paper
• Cloning and characterization of hdlg: the human homologue of the Drosophila discs large tumor suppressor binds to protein 4.1.

  1994cited by this paper
• Association of polyomavirus middle tumor antigen with 14-3-3 proteins.

  1994cited by this paper
• 14-3-3: modulators of signaling proteins?

  1994cited by this paper
• Src kinase associates with a member of a distinct subfamily of protein-tyrosine phosphatases containing an ezrin-like domain.

  1994cited by this paper
• Molecular cloning of a novel protein‐tyrosine phosphatase containing a membrane‐binding domain and GLGF repeats

  1994cited by this paper
• Membrane-actin microfilament connections: an increasing diversity of players related to band 4.1.

  1994cited by this paper
• Identification of a novel protein tyrosine phosphatase with sequence homology to the cytoskeletal proteins of the band 4.1 family

  1994cited by this paper
• Interaction of the protein kinase Raf-1 with 14-3-3 proteins.

  1994cited by this paper
• Protein phosphorylation: a practical approach

  1993cited by this paper
• Casein kinase II phosphorylates p34cdc2 kinase in G1 phase of the HeLa cell division cycle.

  1992cited by this paper
• Identification, cloning, and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to cytoskeletal protein 4.1.

  1991cited by this paper
• Isolation of a cDNA clone encoding a human protein-tyrosine phosphatase with homology to the cytoskeletal-associated proteins band 4.1, ezrin, and talin.

  1991cited by this paper

• Protein tyrosine phosphatase PTPH1 potentiates receptor tyrosine kinase HER2 oncogenesis via a PDZ-coupled and phosphorylation-driven scaffold.

  2024cites this paper
• Cardiomyocyte-specific disruption of the circadian BMAL1–REV-ERBα/β regulatory network impacts distinct miRNA species in the murine heart

  2023cites this paper
• MARK3 kinase: Regulation and physiologic roles.

  2022cites this paper
• Comparative Phosphoproteomic Analysis Reveals the Response of Starch Metabolism to High-Temperature Stress in Rice Endosperm

  2021cites this paper
• Molecular basis of the interaction of the human tyrosine phosphatase PTPN3 with the hepatitis B virus core protein

  2021cites this paper
• Signature selection analysis reveals candidate genes associated with production traits in Iranian sheep breeds

  2021cites this paper
• Large-Scale Phosphoproteomic Study of Arabidopsis Membrane Proteins Reveals Early Signaling Events in Response to Cold

  2020cites this paper
• Global Phosphoproteomic Analysis Reveals the Defense and Response Mechanisms of Jatropha Curcas Seedling under Chilling Stress

  2019cites this paper
• The Protein Tyrosine Phosphatase H1 PTPH1 Supports Proliferation of Keratinocytes and is a Target of the Human Papillomavirus Type 8 E6 Oncogene

  2019influential citation
• 14-3-3 proteins from Vitis vinifera bind to and modulate the anthocyanidin synthase activity in vitro

  2018cites this paper
• Microtubule affinity-regulating kinases are potential druggable targets for Alzheimer’s disease

  2017cites this paper
• Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.

  2017cites this paper
• Knockout of 4.1B triggers malignant transformation in SV40T‐immortalized mouse embryo fibroblast cells

  2017cites this paper
• Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco.

  2017influential citation
• Non-transmembrane PTPs in Cancer

  2016cites this paper
• GIRK Channels: A Potential Link Between Learning and Addiction.

  2015cites this paper
• Chaperone-mediated specificity in Ras and Rap signaling

  2015cites this paper
• Genome-Wide Association Study of Staphylococcus aureus Carriage in a Community-Based Sample of Mexican-Americans in Starr County, Texas

  2015cites this paper
• PAR-1 Kinase and Cell Polarity

  2015cites this paper
• Etude structurale et fonctionnelle de la phosphatase humaine PTPN4

  2014cites this paper
• Cytosolic Protein Phosphatases

  2013cites this paper
• Cytoplasmic Protein Tyrosine Kinases

  2013cites this paper
• Cytoplasmic Protein Serine/Threonine Kinases

  2013cites this paper
• Preamble to Cytoplasmic Protein Kinases

  2013cites this paper
• Guanosine Triphosphatases and Their Regulators

  2013cites this paper
• Other Major Types of Signaling Mediators

  2013cites this paper
• Dual-Specificity Protein Kinases

  2013cites this paper
• Mitogen-Activated Protein Kinase Module

  2013cites this paper
• Biochemical and Phosphoproteomic Analysis of the Helix-Loop-Helix Protein E47

  2012cites this paper
• A characterisation and functional analysis of the role of the 14-3-3-like proteins in neuronal ageing in the pond snail, Lymnaea stagnalis

  2012cites this paper
• SLiMPrints: conservation-based discovery of functional motif fingerprints in intrinsically disordered protein regions

  2012cites this paper
• Microtubule affinity-regulating kinase 4 (MARK4) is a component of the ectoplasmic specialization in the rat testis

  2012cites this paper
• 3 The Cardiac Ion Channels

  2012cites this paper
• The interactome of a PTB domain-containing adapter protein, Odin, revealed by SILAC.

  2011cites this paper
• Regulation and physiological function of Nav1.5 and KCNQ1 channels

  2011cites this paper
• Involvement of 14-3-3 protein post-translational modifications in Giardia duodenalis encystation.

  2010cites this paper
• Loss of Par-1a/MARK3/C-TAK1 Kinase Leads to Reduced Adiposity, Resistance to Hepatic Steatosis, and Defective Gluconeogenesis

  2010cites this paper
• Identification and characterization of PKD2 substrates - CIB1A as a substrate for PKD2

  2010cites this paper
• Protein Tyrosine Phosphatase H1 (PTPH1) Inhibits LPS-induced TNF-α Production

  2010cites this paper
• Structure and function of polarity‐inducing kinase family MARK/Par‐1 within the branch of AMPK/Snf1‐related kinases

  2010cites this paper
• Differential 14-3-3 Affinity Capture Reveals New Downstream Targets of Phosphatidylinositol 3-Kinase Signaling*

  2009cites this paper
• TTP at Ser245 phosphorylation by AKT is required for binding to 14-3-3.

  2009cites this paper
• Role of the Protein 14-3-3 in Spermatogenesis and Sperm Motility

  2009cites this paper
• The tau of MARK: a polarized view of the cytoskeleton.

  2009cites this paper
• Towards understanding the role of the ubiquitinligase E6AP in human disease

  2009cites this paper
• Behavioral and Brain Functions

  2008cites this paper
• Phosphorylation-Dependent Interaction of Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein (YWHA) with PADI6 Following Oocyte Maturation in Mice1

  2008cites this paper
• Modulation of 14-3-3 interaction with phosphorylated histone H3 by combinatorial modification patterns

  2008cites this paper
• Checkpoint Regulation of S-Phase Transcription: A Dissertation

  2008cites this paper
• The SERK1 protein complexes

  2008cites this paper
• Suppression of hepatitis B viral gene expression by protein-tyrosine phosphatase PTPN3.

  2007cites this paper
• Regulatory mechanisms of the plant G2 M transition

  2007cites this paper
• Protein tyrosine phosphatase H1 is a target of the E6 oncoprotein of high-risk genital human papillomaviruses.

  2007influential citation
• Extending from PARs in Caenorhabditis elegans to homologues in Haemonchus contortus and other parasitic nematodes

  2006cites this paper
• Crystal structure of the catalytic and ubiquitin-associated domains of the protein kinase MARK2 / PAR-1 from Rattus norvegicus (Berkenhout, 1769)

  2006cites this paper
• Cardiac sodium channel Na(v)1.5 interacts with and is regulated by the protein tyrosine phosphatase PTPH1.

  2006cites this paper
• Activity of Class IIa Histone Deacetylases C-TAK 1 in Regulating Localization and New Role for hPar-1 Kinases EMK and

  2006cites this paper
• New Role for hPar-1 Kinases EMK and C-TAK1 in Regulating Localization and Activity of Class IIa Histone Deacetylases

  2006cites this paper
• Phosphorylation of transglutaminase 2 by PKA at Ser216 creates 14‐3‐3 binding sites

  2006cites this paper
• Regulation of the polarity kinases PAR-1/MARK by 14-3-3 interaction and phosphorylation

  2006cites this paper
• Microphthalmia-associated transcription factor interactions with 14-3-3 modulate differentiation of committed myeloid precursors.

  2006cites this paper
• The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 Protein Complex Includes BRASSINOSTEROID-INSENSITIVE1[W]

  2006cites this paper
• The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14λ and the PP2C phosphatase KAPP

  2005cites this paper
• Disruption of 14-3-3 binding does not impair Protein 4.1B growth suppression

  2004cites this paper
• Isoform-specific differences in rapid nucleocytoplasmic shuttling cause distinct subcellular distributions of 14-3-3 sigma and 14-3-3 zeta.

  2004cites this paper
• 14-3-3 Binds to and Mediates Phosphorylation of Microtubule-associated Tau Protein by Ser9-phosphorylated Glycogen Synthase Kinase 3β in the Brain*

  2004cites this paper
• Isoform-specific differences in rapid nucleocytoplasmic shuttling cause distinct subcellular distributions of 14-3-3σ and 14-3-3ζ

  2004cites this paper
• MARKing tau for tangles and toxicity.

  2004cites this paper
• Mild impairment of motor nerve repair in mice lacking PTP-BL tyrosine phosphatase activity.

  2004cites this paper
• 14-3-3 binds to and mediates phosphorylation of microtubule-associated tau protein by Ser9-phosphorylated glycogen synthase kinase 3beta in the brain.

  2004cites this paper
• Anchoring of both PKA and 14‐3‐3 inhibits the Rho‐GEF activity of the AKAP‐Lbc signaling complex

  2004cites this paper
• Threonine 391 Phosphorylation of the Human Prolactin Receptor Mediates a Novel Interaction with 14-3-3 Proteins*

  2003cites this paper
• Self-association of the Spindle Pole Body-related Intermediate Filament Protein Fin1p and Its Phosphorylation-dependent Interaction with 14-3-3 Proteins in Yeast*

  2003cites this paper
• Functional analysis of C‐TAK1 substrate binding and identification of PKP2 as a new C‐TAK1 substrate

  2003cites this paper
• Negative Regulation of Mitotic Promoting Factor by the Checkpoint Kinase Chk1 in Simian Virus 40 Lytic Infection

  2003cites this paper
• The zebrafish band 4.1 member Mir is involved in cell movements associated with gastrulation.

  2003influential citation
• CHAPTER 108 – Overview of Protein Tyrosine Phosphatases

  2003cites this paper
• Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants.

  2002cites this paper
• Localization of PTP-FERM in nerve processes through its FERM domain.

  2002cites this paper
• Protein tyrosine phosphatases.

  2002cites this paper
• The interaction between ADAM22 and 14-3-3β

  2002cites this paper
• How do 14‐3‐3 proteins work? – Gatekeeper phosphorylation and the molecular anvil hypothesis

  2002cites this paper
• Evidence for Regulation of the Tumor Necrosis Factor α-Convertase (TACE) by Protein-tyrosine Phosphatase PTPH1*

  2002cites this paper
• The 4.1/ezrin/radixin/moesin domain of the DAL-1/Protein 4.1B tumour suppressor interacts with 14-3-3 proteins.

  2002cites this paper
• The 14-3-3s

  2002cites this paper
• 14-3-3 interacts with the tumor suppressor tuberin at Akt phosphorylation site(s).

  2002cites this paper
• 14-3-3tau associates with and activates the MEF2D transcription factor during muscle cell differentiation.

  2001cites this paper
• C-TAK1 regulates Ras signaling by phosphorylating the MAPK scaffold, KSR1.

  2001cites this paper
• Identification of a novel interaction between integrin β1 and 14-3-3β

  2001cites this paper
• 14-3-3 associates with and activates the MEF2D transcription factor during muscle cell differentiation

  2001cites this paper
• 14‐3‐3 proteins: key regulators of cell division, signalling and apoptosis

  2001cites this paper
• Association of GABA(B) receptors and members of the 14-3-3 family of signaling proteins.

  2001cites this paper
• Identification of a novel interaction between integrin beta1 and 14-3-3beta.

  2001cites this paper
• Specificity of 14-3-3 isoform dimer interactions and phosphorylation.

  2001cites this paper
• Functional conservation of 14-3-3 isoforms in inhibiting bad-induced apoptosis.

  2001cites this paper
• Regulation of glucocorticoid receptor activity by 14--3-3-dependent intracellular relocalization of the corepressor RIP140.

  2001cites this paper
• Regulation of BAD by cAMP-dependent protein kinase is mediated via phosphorylation of a novel site, Ser155.

  2000cites this paper
• 14-3-3zeta interacts with the alpha-chain of human interleukin 9 receptor.

  2000cites this paper
• Integration of Calcium and Cyclic AMP Signaling Pathways by 14-3-3

  2000cites this paper
• 14-3-3 proteins: structure, function, and regulation.

  2000cites this paper