Specific glucocorticoid receptors in thymus cells. Localization in the nucleus and extraction of the cortisol-receptor complex.

Abstract Specific glucocorticoid receptors previously identified in rat thymus cells by measurements of steroid binding to whole cells are found to be located largely, if not entirely, in the nucleus. Localization is accomplished by showing that cortisol-3H, that is specifically bound to whole cells, remains with the nuclei (identified as such by electron microscopy) after cell membranes and cytoplasmic materials are removed by hypotonic shock in 1.5 mm MgCl2 at 3°. The cortisol-receptor complex has been extracted from nuclei and separated from free cortisol by gel chromatography. It is rapidly degraded by Pronase. The rate at which cortisol dissociated at 37° from the isolated complex, and from nuclei, is indistinguishable from the characteristic slow rate at which specifically bound cortisol dissociates from whole cells, showing that this latter rate is an intrinsic property of the cortisol-receptor complex. The rate at which cortisol associates specifically with whole cells at 37° is indistinguishable from the rate at which it appears in the nuclear receptor complex.

• Publication year

  1970

• Venue

  Journal of Biological Chemistry

• Publication date

  1970-07-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S0021-9258(18)63015-7PMID 5459644
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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