Cellular immunoadsorbents: a simplified technique for separation of lymphoid cell populations.

A simplified and efficient technique for separating T lymphoid cells from non-T cells of mouse, rat and man has been developed. The fractionation procedure is based on the capacity of non-T lymphoid cells to bind antigen-antibody complexes through an Fc receptor. Lymphoid cells are adsorbed on antibody-coated sheep erythrocyte (EA) monolayers prepared in polystyrene Petri dishes previously treated with poly-l-lysine. Adsorption of spleen cells on these monolayers, assisted by a 5-min centrifugation, resulted in 90 to 98% depletion of the Fc receptor-bearing cells (mostly B lymphocytes, but also macrophages and polymorphonuclear leukocytes) in the non-adherent cell population. Eighty to 90% of the adherent cells were capable of forming EA rosettes and could be recovered after lysis of the erythrocytes. The non-adherent fraction comprised at least 90% T-cells. This was determined for mouse and rat spleen cells by lysis with anti-thymocyte serum and complement, and by the absence of immunoglobulin-bearing cells. The specificity of the adsorption was further substantiated by the following observations: 1) lymphoid cells lacking an Fc receptor were not adsorbed; 2) the adsorption to EA monolayers was inhibited by antigen-antibody complexes or by aggregated γ-globulin; and 3) only 10 to 15% of the Fc receptor-bearing cells were attached to non-sensitized erythrocyte (E) monolayers. Fractionation of spleen cells obtained from rats immunized against a syngeneic tumor yielded two separate fractions of cytotoxic lymphoid cells: one (non-adherent, T cells) mediating direct target cell destruction, and the other (adherent, non-T cells) mediating lysis of antibody-coated target cells.

• Publication year

  1974

• Venue

  Journal of Immunology

• Publication date

  1974-03-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.112.3.1231PMID 4590702
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Enzymatic enhancement of cell-mediated cytotoxicity and antibody-dependent cell cytotoxicity.

  1974cited by this paper
• Characterization of the antibody-dependent cytotoxic cell. A non-phagocytic monocyte?

  1973cited by this paper
• NATURE OF LYMPHOCYTE-TUMOR INTERACTION

  1973cited by this paper
• Antibody-dependent cell-mediated cytotoxicity due to a "null" lymphoid cell.

  1973cited by this paper
• SHEEP RED CELL BINDING TO HUMAN LYMPHOCYTES TREATED WITH NEURAMINIDASE; ENHANCEMENT OF T CELL BINDING AND IDENTIFICATION OF A SUBPOPULATION OF B CELLS

  1973cited by this paper
• Receptor for antibody-antigen complexes used to separate T cells from B cells.

  1972cited by this paper
• In vivo studies of the role of cytotoxic T cells in tumor allograft immunity.

  1972cited by this paper
• Specificiy of lymphocyte-mediated cytotoxicity induced by in vitro antibody-coated target cells.

  1972cited by this paper
• A RECEPTOR FOR ANTIBODY ON B LYMPHOCYTES

  1972cited by this paper
• Study of Fab and F(ab') 2 from rabbit IgG for capacity to induce lymphocyte-mediated target cell destruction in vitro.

  1972cited by this paper
• Specific adsorption of cytotoxic thymus‐processed lymphocytes (T cells) on glutaraldehyde‐fixed fibroblast monolayers

  1972cited by this paper
• A RECEPTOR FOR ANTIBODY ON B LYMPHOCYTES

  1972cited by this paper
• Autonomy of thymus‐processed lymphocytes (T cells) for their education into cytotoxic cells

  1972cited by this paper
• Interaction between lymphocytes in immune responses.

  1971cited by this paper
• An improved assay for haemolytic plaque-forming cells.

  1971cited by this paper
• A POPULATION OF LYMPHOCYTES BEARING A MEMBRANE RECEPTOR FOR ANTIGEN-ANTIBODY-COMPLEMENT COMPLEXES

  1970cited by this paper
• The IgG receptor: an immunological marker for the characterization of mononuclear cells.

  1969cited by this paper
• AN EXTENSION OF THE51Cr-RELEASE ASSAY FOR THE ESTIMATION OF MOUSE CYTOTOXINS

  1968cited by this paper

• Past, Present, and Future of Affinity-based Cell Separation Technologies.

  2020cites this paper
• TUBE LEUKOCYTE ADHERENCE INHIBITION ASSAY FOR THE THE REACTIVE CELL DETECTION OF ANTI-TUMOUR TMMUNITY. I. MONOCYTE IS

  2006cites this paper
• Effector cells in natural cytotoxicity against human bladder cancer cell lines

  2004cites this paper
• ACTIVATION OF Fc RECEPTOR-BEARING LYMPHOCYTES BY IMMUNE COMPLEXES II. Killer Lymphocytes Mediate Fc Ligand-induced Lymphokine Production*

  2003influential citation
• The effect of serotonin interaction with cells

  1993cites this paper
• Suppressive effect of a mouse testicular extract on lymphocyte activation.

  1991cites this paper
• Target and effector cell fusion accounts for B lymphocyte-mediated lysis of mouse hepatitis virus-infected cells.

  1989cites this paper
• Cytotoxic T lymphocytes in reticuloendotheliosis virus‐infected chickens

  1989cites this paper
• B cells from chronic lymphocytic leukemia (CLL) patients are strong inducers of proliferation and major histocompatibility complex (MHC)-unrestricted [natural killer (NK)-like] cytotoxicity in normal T-lymphocytes

  1989cites this paper
• Prolactin receptors on large granular lymphocytes: dual regulation by cyclosporin A.

  1988cites this paper
• Participation of serotoninergic system in neuroimmunomodulation: intraimmune mechanisms and the pathways providing an inhibitory effect.

  1988cites this paper
• Dopaminergic stimulation of the immune reaction: interaction of serotoninergic and dopaminergic systems in neuroimmunomodulation.

  1988cites this paper
• Effect of cortisol on the native and in vitro induced non-MHC restricted cytotoxicity of large granular lymphocytes.

  1988cites this paper
• PROLONGATION OF GRAFT SURVIVAL IN ALS‐TREATED MICE BY DONOR‐SPECIFIC BONE MARROW: DENSITY GRADIENT FRACTIONATION OF THE ACTIVE BONE MARROW CELLS

  1987cites this paper
• Generation of non MHC restricted killing in non‐cytotoxic T‐lymphocytes from leukaemia patients

  1987cites this paper
• Distribution of Immunocompetent Cells Underlying Psychoneuroimmunomodulation. Brain Neuromediator Control Mechanisms

  1987cites this paper
• Modulation of in vitro myelopoiesis by LGL: different effects on early and late progenitor cells.

  1986cites this paper
• Natural killer cells in the blood and bone marrow of the rhesus monkey.

  1986cites this paper
• Monoamines as immunomodulators: importance of suppressors and helpers of the bone marrow.

  1986cites this paper
• Monoclonal antibodies to human renal cell carcinoma: recognition of shared and restricted tissue antigens.

  1985cites this paper
• Cytotoxic antibody dependent cells in mice experimentally infected with Brucella abortus.

  1985cites this paper
• Migration of natural suppressor cells from bone marrow to peritoneal cavity by live BCG.

  1985cites this paper
• Suppression of human NK cell cytotoxicity by an MLC-Generated cell population.

  1985cites this paper
• Cytotoxic lymphocytes generated in vivo with acute measles virus infection.

  1985cites this paper
• Mouse skin graft prolongation with donor strain bone marrow and anti-lymphocyte serum: surface markers of the active bone marrow cells.

  1985cites this paper
• IgA-dependent cell-mediated activity against enteropathogenic bacteria: distribution, specificity, and characterization of the effector cells.

  1984cites this paper
• Immune response against vaccinia virus in rhesus monkeys: no evidence for primary MHC-restricted cytolytic T cells.

  1984cites this paper
• [Serotonin binding by various populations of peritoneal cells and blood leukocytes from intact and thymectomized mice].

  1984cites this paper
• [12] Cell separation on cellular monolayers

  1984cites this paper
• Cell separation on cellular monolayers.

  1984cites this paper
• Suppression of autoantibodies is specific for the foreign antigens inducing the autoimmunity.

  1983cites this paper
• Natural and antibody-dependent cell-mediated activity against Salmonella typhimurium by peripheral and intestinal lymphoid cells in mice.

  1983cites this paper
• Characterization of Lymphocytes Responsible for Protective Immunity to Histoplasmosis in Mice * /Charakterisierung von Lymphozyten der protektiven Immunität gegen Histoplasmose bei Mäusen

  1983cites this paper
• Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species.

  1983cites this paper
• Involvement of macrophage-like cells in growth of tumors induced by avian sarcoma virus.

  1983cites this paper
• A human NK and K cell subset shares with cytotoxic T cells expression of the antigen recognized by antibody OKT8.

  1983cites this paper
• Characterization of Marek's disease virus-infected lymphocytes: discrimination between cytolytically and latently infected cells.

  1983cites this paper
• Involvement of Macrophage-like Cellsin Growthof TumorsInducedby AvianSarcomaVirus1

  1983cites this paper
• Immune destruction of rabbit corneal cells infected with herpes simplex virus: lymphocyte reactivity by antibody-dependent cellular cytotoxicity.

  1982cites this paper
• Studies on cytotoxicity generated in human mixed lymphocyte cultures. III. Natural killer-like cytotoxicity mediated by human lymphocytes with receptors for IgM.

  1982cites this paper
• MORPHOLOGICAL CHARACTERISTICS OF INTERFERON-PRODUCING CELLS IN HUMAN PERIPHERAL BLOOD

  1982cites this paper
• Tumor enhancing T lymphocytes in mice: Further studies on characteristics and mechanism of activity

  1982cites this paper
• Demonstration of primary cytotoxic T cells in venous blood and cerebrospinal fluid of children with mumps meningitis.

  1982cites this paper
• Surface antigens on Marek's disease lymphoblastoid tumor cell lines.

  1982cites this paper
• Mechanisms of killing of measles virus-infected cells by human lymphocytes: interferon associated and unassociated cell-mediated cytotoxicity.

  1982cites this paper
• Modulation of antitumoral antibody-dependent cellular cytotoxicity and natural killer activity by Adriamycin and daunorubicin

  1982cites this paper
• Activation of Fc receptor-bearing lymphocytes by immune complexes. I. Stimulation of lymphokine production by nonadherent human peripheral blood lymphocytes.

  1982cites this paper
• Systemic administration of human leukocyte interferon to melanoma patients. II. Cellular events associated with changes in natural killer cytotoxicity.

  1982cites this paper
• Rat major histocompatibility RT1.C antigens of restricted tissue distribution consist of two polypeptide chains with molecular weights of about 42 000 and 12 500

  1982cites this paper
• Modulation of antltumoral antibody-dependent cellular eytotoxicity and natural Miler activity by Adriamyeln and daunorubicin j

  1982cites this paper
• Serologic and cellular characterization of products of a new major histocompatibility gene region, RT1.C, of the rat; possible homology to mouse H-2 Qa.

  1982cites this paper
• Detection of double target binders at the single cell level: an evaluation of the specificity of NK and MLC-induced NK-like cells.

  1982cites this paper
• Characterization of non-oncogenic Marek's disease virus-infected and turkey herpesvirus-infected lymphocytes.

  1982cites this paper
• HLA restriction of secondary mumps-specific cytotoxic T lymphocytes.

  1982cites this paper
• A rapid technique for isolation of viable tumor cells from solid tumors: use of the tumor cells for induction and measurement of cell-mediated cytotoxic responses.

  1982cites this paper
• Failure to detect killer cell activity in rabbits

  1982cites this paper
• INDUCTION OF SUPPRESSOR CELLS BY DONOR‐SPECIFIC BLOOD TRANSFUSIONS AND HEART TRANSPLANTATION IN RATS

  1981cites this paper
• Binding of C-reactive protein to human lymphocytes. II. Interaction with a subset of cells bearing the Fc receptor.

  1981cites this paper
• Spontaneous autoimmune thyroiditis in the rat accelerated by thymectomy and low doses of irradiation: mechanisms implicated in the pathogenesis.

  1981cites this paper
• Activation of Fc receptor-bearing lymphocytes by immune complexes. II. Killer lymphocytes mediate Fc ligand-induced lymphokine production

  1981influential citation
• Enzyme-linked immunosorbent assay (ELISA) to detect antibodies in gonorrhea using whole cells.

  1981cites this paper
• Phenotypic and functional characterization of allospecific and nonspecific (NK- and K-like) cytotoxic T lymphocytes generated in human mixed-lymphocyte cultures from noncytotoxic precursors

  1981cites this paper
• T cell-mediated cytotoxicity induced by Listeria monocytogenes. III. Phenotypic characteristics of mediator T cells.

  1981cites this paper
• Antitumor immunity in B-lymphocyte-deprived mice. II. In vitro studies.

  1981cites this paper
• A sensitive solid phase microradioimmunoassay for anti-double stranded DNA antibodies.

  1981cites this paper
• Early precursors of B lymphocytes II. Exploitation of the singular properties of rabbit pre‐B cells in their purification

  1981cites this paper
• [Nonspecific suppressor cells in the inhibiting action of serotonin on immunogenesis].

  1981cites this paper
• Natural killer (NK) cells in hamsters and their modulation in tumorigenesis.

  1981cites this paper
• Cell-mediated immunity in interstitial nephritis. IV. Anti-tubular basement membrane antibodies can function in antibody-dependent cellular cytotoxicity reactions: observations on a nephritogenic effector mechanism acting as an informational bridge between the humoral and cellular immune response.

  1981cites this paper
• Plaque-forming, antibody-dependent, cytotoxic cells with monocytic properties are present in rheumatoid synovial tissue.

  1981cites this paper
• Natural killing of cytomegalovirus-infected fibroblasts by human mononuclear leucocytes.

  1981cites this paper
• Epstein Barr virus selective T cells in infectious mononucleosis are not restricted to HLA-A and B antigens.

  1981cites this paper
• Enzyme Immunoassays in Bacteriology

  1981cites this paper
• K cell activity of murine-cultured lymphocytic leukemic cell line independent of other Fc receptor functions.

  1981cites this paper
• Murine mammary tumor virus deficient in the major glycoprotein: biochemical and biological studies on virions produced by a lymphoma cell line.

  1980cites this paper
• CHARACTERISTICS OF NK AND RELATED CELLS

  1980cites this paper
• Methods of Cell Separation

  1980cites this paper
• Augmentation of human natural killer cell activity by polyinosinic acid-polycytidylic acid and its nontoxic mismatched analogues.

  1980cites this paper
• A comparative analysis of the cell surface properties of activated vs endogenous mouse natural killer cells.

  1980cites this paper
• Human monocyte spreading induced by factor B of the alternative pathway of complement activation.

  1980cites this paper
• Inhibition of an in vitro antibody response by a suppressor cell in normal bone marrow.

  1980cites this paper
• Inhibition by autologous lymphoid cells of antibody-dependent cellular cytotoxicity (ADCC) to a tumor target.

  1980cites this paper
• Quantitative studies of natural immunity to solid tumours in rats. The nature of the killer cell depends on the type of assay.

  1980cites this paper
• The bacteria(B)--antibody(A)--complement(C) (BAC) rosette method for detecting C3 receptors (R): binding specificity and capping of human peripheral blood lymphocyte C3r.

  1980cites this paper
• A unique antigen (sigma) on sézary cells

  1980cites this paper
• ARE SPONTANEOUS AND ANTIBODY-DEPENDENT LYSIS TWO DIFFERENT MECHANISMS OF CYTOTOXICITY MEDIATED BY THE SAME CELL?*

  1980cites this paper
• Adjuvant-induced nonspecific supressor cells: in vitro and in vivo studies.

  1980cites this paper
• Interferon production by human and murine lymphocytes in response to alloantigens.

  1980cites this paper
• Transfer of natural resistance to Marek's disease (JMV) with nonimmune spleen cells. II. Further characterization of protecting cell population.

  1980cites this paper
• Breast milk lymphocyte response to K1 antigen of Escherichia coli

  1980cites this paper
• The participation of human peripheral blood lymphocytes with Fc receptors for IgG or IgM in the fetal bovine serum induction of amplifier cell activity.

  1980cites this paper
• Enhancement of spontaneous killer cytotoxicity by soluble factor.

  1980cites this paper
• Biological Methods for the Separation of Lymphoid Cells

  1980cites this paper
• AUGMENTATION OF HUMAN NATURAL KILLER CELL ACTIVITY BY PURIFIED INTERFERON AND POLYRIBONUCLEOTIDES1

  1980cites this paper
• In vitro induction of cell-mediated immunity to murine leukemia cells. VII. Methods for augmenting the induction and expression of cytotoxic response in vitro to syngeneic tumors.

  1979cites this paper
• Activation of human natural killer cells cytotoxic for human leukemia cells by purified interferon.

  1979cites this paper
• Antigen and mitogen induced production of macrophage migration inhibitory factor in the mouse.

  1979cites this paper
• Mechanism of cell-mediated cytotoxicity at the single cell level. I. Estimation of cytotoxic T lymphocyte frequency and relative lytic efficiency.

  1979cites this paper
• The effect of adjuvant induced suppressor cells on in vitro and in vivo lymphocyte responses.

  1979cites this paper
• Lipid synthesis: an indicator of antigen-induced signal transduction in antigen-binding cells.

  1979cites this paper