Signal recognition particle: a ribonucleoprotein required for cotranslational translocation of proteins, isolation and properties.

No abstract is available for this paper.

• Publication year

  1983

• Venue

  Methods in Enzymology

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0076-6879(83)96057-3PMID 6197610
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Methods for the study of protein translocation across the RER membrane using the reticulocyte lysate translation system and canine pancreatic microsomal membranes.

  1983cited by this paper
• Isolation of small nuclear ribonucleoproteins containing U1, U2, U4, U5, and U6 RNAs.

  1983cited by this paper
• Preparation of microsomal membranes for cotranslational protein translocation.

  1983cited by this paper
• Cell-free translation of messenger RNA in a wheat germ system.

  1983cited by this paper
• Protein translocation across the endoplasmic reticulum. II. Isolation and characterization of the signal recognition particle receptor

  1982cited by this paper
• Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum

  1982cited by this paper
• Secretory protein translocation across membranes—the role of the ‘docking protein’

  1982cited by this paper
• Human 7SL RNA consists of a 140 nucleotide middle-repetitive sequence inserted in an alu sequence.

  1982cited by this paper
• Signal recognition protein is required for the integration of acetylcholine receptor delta subunit, a transmembrane glycoprotein, into the endoplasmic reticulum membrane

  1982cited by this paper
• Nucleotide sequence of 7 S RNA. Homology to Alu DNA and La 4.5 S RNA.

  1982cited by this paper
• Protein translocation across the endoplasmic reticulum. I. Detection in the microsomal membrane of a receptor for the signal recognition particle

  1982cited by this paper
• Translocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein

  1981cited by this paper
• Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site- specific arrest of chain elongation that is released by microsomal membranes

  1981cited by this paper
• Translocation of proteins across the endoplasmic reticulum. II. Signal recognition protein (SRP) mediates the selective binding to microsomal membranes of in-vitro-assembled polysomes synthesizing secretory protein

  1981cited by this paper
• Secretion requires a cytoplasmically disposed sulphydryl of the RER membrane

  1980cited by this paper
• Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.

  1980cited by this paper
• Ribonuclease inhibitor from human placenta: rapid purification and assay.

  1979cited by this paper
• Protein transfer across microsomal membranes reassembled from separated membrane components

  1978cited by this paper
• Omega-aminoalkyl agaroses in the resolution of enzymes involved in regulation of glutamine metabolism.

  1975cited by this paper
• Isolation of a 5S RNA-protein complex from mammalian ribosomes.

  1971cited by this paper

• Temporal Regulation of Signal Recognition Particle During Translation

  2025cites this paper
• Role of Hsp70 in Post-Translational Protein Targeting: Tail-Anchored Membrane Proteins and Beyond

  2023cites this paper
• Receptor compaction and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER

  2021cites this paper
• Antibiotic Drug screening and Image Characterization Toolbox (A.D.I.C.T.): a robust imaging workflow to monitor antibiotic stress response in bacterial cells in vivo

  2021cites this paper
• Share the details of your experimental methods

  2020cites this paper
• Receptor compaction and GTPase movements drive cotranslational protein translocation

  2020cites this paper
• Reconstitution of the human SRP system and quantitative and systematic analysis of its ribosome interactions

  2019cites this paper
• Pathogenic Signal Sequence Mutations in Progranulin Disrupt SRP Interactions Required for mRNA Stability

  2018cites this paper
• The genetics and molecular biology of fever-associated seizures or epilepsy

  2018cites this paper
• In vivo characterization of TMX1: TMX1 preferentially acts upon transmembrane polypeptides

  2016cites this paper
• The code for directing proteins for translocation across ER membrane: SRP cotranslationally recognizes specific features of a signal sequence.

  2015cites this paper
• Inefficient SRP interaction with a nascent chain triggers a mRNA quality control pathway.

  2014cites this paper
• Mff functions with Pex11pβ and DLP1 in peroxisomal fission

  2013influential citation
• Structural and Functional Analysis of the Molecular Chaperone Calnexin

  2010cites this paper
• Mammalian signal recognition particle: Involvement of both the SRP9 and the SRP14 proteins in the elongation arrest activity

  2009influential citation
• Mechanistic insights into the protein targeting mediated by signal recognition particle

  2008cites this paper
• Post-translational integration of tail-anchored proteins is facilitated by defined molecular chaperones

  2007influential citation
• Real-Time Fluorescence Detection of ERAD Substrate Retrotranslocation in a Mammalian In Vitro System

  2007cites this paper
• Structures of protein targeting complexes

  2006cites this paper
• Ribosome binding to and dissociation from translocation sites of the endoplasmic reticulum membrane.

  2006influential citation
• Human autoantibodies against the 54 kDa protein of the signal recognition particle block function at multiple stages

  2006cites this paper
• The origin and maintenance of mammalian peroxisomes involves a de novo PEX16-dependent pathway from the ER

  2006cites this paper
• N-myristoylation determines dual targeting of mammalian NADH-cytochrome b(5) reductase to ER and mitochondrial outer membranes by a mechanism of kinetic partitioning

  2005cites this paper
• Selective SecA Association with Signal Sequences in Ribosome-bound Nascent Chains

  2005cites this paper
• The mammalian signal recognition particle : new insights into mechanism and activation of its elongation arrest activity

  2005influential citation
• Signal recognition particle mediates post‐translational targeting in eukaryotes

  2004cites this paper
• Signal recognition particle Alu domain occupies a defined site at the ribosomal subunit interface upon signal sequence recognition.

  2004cites this paper
• Structure of the signal recognition particle interacting with the elongation-arrested ribosome

  2004cites this paper
• Signal Recognition Particle Mediates a Transient Elongation Arrest of Preprolactin in Reticulocyte Lysate

  2003cites this paper
• The fl Subunit of the Signal Recognition Particle Receptor Is a Transmembrane GTPase that Anchors the a Subunit, a Peripheral Membrane GTPase, to the Endoplasmic Reticulum Membrane

  2002cites this paper
• Membrane topogenesis of the three amino-terminal transmembrane segments of glucose-6-phosphatase on endoplasmic reticulum.

  2002cites this paper
• Characterization of the ft sYEX operon of Escherichia coli

  2002cites this paper
• Biochemical analysis of the yeast SRP receptor

  2002cites this paper
• The amino terminus of opsin translocates "posttranslationally" as efficiently as cotranslationally.

  2002cites this paper
• The tenth membrane region of band 3 is initially exposed to the luminal side of the endoplasmic reticulum and then integrated into a partially folded band 3 intermediate.

  2002cites this paper
• Targeting of the hepatitis B virus precore protein to the membrane: after signal

  2002cites this paper
• Characterization of the ftsYEX operon of Escherichia coli

  2002cites this paper
• In Vitro Translation of the Angiotensin AT(1) Receptor in Wheat-Germ Extracts.

  2001influential citation
• Membrane Topography and Topogenesis of Prenylated Rab Acceptor (PRA1)*

  2001cites this paper
• The Interaction of the Chaperonin Tailless Complex Polypeptide 1 (Tcp1) Ring Complex (Tric) with Ribosome-Bound Nascent Chains Examined Using Photo-Cross-Linking

  2000cites this paper
• Role of ubiquitin in proteasomal degradation of mutant alpha(1)-antitrypsin Z in the endoplasmic reticulum.

  2000cites this paper
• AGI January 41/1

  2000cites this paper
• Oligomeric complexes involved in translocation of proteins across the membrane of the endoplasmic reticulum

  1999cites this paper
• Membrane topology of NADPH-cytochrome P450 reductase on the endoplasmic reticulum.

  1998cites this paper
• The transient association of ERp57 with N-glycosylated proteins is regulated by glucose trimming.

  1998cites this paper
• Coexpression of G-CSF with an unglycosylated G-CSF receptor mutant results in secretion of a stable complex.

  1998cites this paper
• A general mechanism for regulation of access to the translocon: competition for a membrane attachment site on ribosomes.

  1998cites this paper
• Expression of dendritic BC200 RNA, component of a 11.4S ribonucleoprotein particle, is conserved in humans and simians.

  1997cites this paper
• The hydrophobic region of signal peptides is a determinant for SRP recognition and protein translocation across the ER membrane.

  1997cites this paper
• Sequence-specific alteration of the ribosome-membrane junction exposes nascent secretory proteins to the cytosol.

  1996cites this paper
• A Nascent Secretory Protein 5 Traverse the Ribosome/Endoplasmic Reticulum Translocase Complex as an Extended Chain (*)

  1996cites this paper
• A two‐step recognition of signal sequences determines the translocation efficiency of proteins.

  1996cites this paper
• Identification and characterization of BC1 RNP particles.

  1996cites this paper
• Reinitiation of Protein Translocation across the Endoplasmic Reticulum Membrane for the Topogenesis of Multispanning Membrane Proteins (*)

  1996cites this paper
• A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins.

  1995cites this paper
• Targeting and topology in the membrane of plant 3-hydroxy-3-methylglutaryl coenzyme A reductase.

  1995cites this paper
• four-jointed is required for intermediate growth in the proximal-distal axis in Drosophila.

  1995cites this paper
• The SRP9/14 subunit of the signal recognition particle (SRP) is present in more than 20-fold excess over SRP in primate cells and exists primarily free but also in complex with small cytoplasmic Alu RNAs.

  1995cites this paper
• Signal Sequence Processing in Rough Microsomes (*)

  1995cites this paper
• Interaction of Calreticulin with Protein Disulfide Isomerase (*)

  1995cites this paper
• Nascent-polypeptide-associated complex: a bridge between ribosome and cytosol.

  1995cites this paper
• The translocation, folding, assembly and redox-dependent degradation of secretory and membrane proteins in semi-permeabilized mammalian cells.

  1995cites this paper
• The intrinsic ability of ribosomes to bind to endoplasmic reticulum membranes is regulated by signal recognition particle and nascent-polypeptide-associated complex.

  1995cites this paper
• The Sec61 complex is essential for the insertion of proteins into the membrane of the endoplasmic reticulum

  1995cites this paper
• Nascent polypeptide-associated complex protein prevents mistargeting of nascent chains to the endoplasmic reticulum.

  1995cites this paper
• The beta subunit of the signal recognition particle receptor is a transmembrane GTPase that anchors the alpha subunit, a peripheral membrane GTPase, to the endoplasmic reticulum membrane

  1995cites this paper
• A homologous cell‐free system for studying protein translocation across the endoplasmic reticulum membrane in fission yeast

  1994cites this paper
• A protein complex required for signal-sequence-specific sorting and translocation

  1994cites this paper
• Genetic and biochemical analysis of the fission yeast ribonucleoprotein particle containing a homolog of Srp54p

  1994cites this paper
• The COOH-terminal ends of internal signal and signal-anchor sequences are positioned differently in the ER translocase

  1994cites this paper
• The prosomal RNA-binding protein p27K is a member of the α-type human prosomal gene family

  1993cites this paper
• Interactions of calreticulin with proteins of the endoplasmic and sarcoplasmic reticulum membranes

  1993cites this paper
• Discrete nascent chain lengths are required for the insertion of presecretory proteins into microsomal membranes

  1993cites this paper
• The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation.

  1993cites this paper
• Role of Lipids and Membrane Biogenesis in the Process of Mucus Secretion

  1993cites this paper
• Die Bedeutung der Lipide und der Membranbiogenese bei der Schleimsekretion

  1993cites this paper
• The prosomal RNA-binding protein p27K is a member of the alpha-type human prosomal gene family.

  1993cites this paper
• A novel short consensus repeat-containing molecule is related to human complement factor H.

  1993cites this paper
• Membrane topology and biogenesis of eukaryotic signal peptidase.

  1993cites this paper
• GTP binding and hydrolysis by the signal recognition particle during initiation of protein translocation

  1993cites this paper
• GTPase domain of the 54-kD subunit of the mammalian signal recognition particle is required for protein translocation but not for signal sequence binding

  1993cites this paper
• Sea urchin small RNA ribonucleoprotein particles: Identification, synthesis, and subcellular localization during early embryonic development

  1992cites this paper
• The signal sequence receptor, unlike the signal recognition particle receptor, is not essential for protein translocation

  1992cites this paper
• Apical secretion and association of the Drosophila yellow gene product with developing larval cuticle structures during embryogenesis

  1992cites this paper
• Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex

  1992cites this paper
• Fluorescence-detected assembly of the signal recognition particle: binding of the two SRP protein heterodimers to SRP RNA is noncooperative.

  1992cites this paper
• Translocation of preproinsulin across the endoplasmic reticulum membrane. The relationship between nascent polypeptide size and extent of signal recognition particle-mediated inhibition of protein synthesis.

  1992cites this paper
• An exported protein of Plasmodium falciparum is synthesized as an integral membrane protein.

  1991cites this paper
• The signal sequence interacts with the methionine-rich domain of the 54- kD protein of signal recognition particle

  1991cites this paper
• [Specific suppressor T-cells secrete RNA].

  1991cites this paper
• A nascent membrane protein is located adjacent to ER membrane proteins throughout its integration and translation

  1991cites this paper
• Biochemical fractionation and assembly of the membrane components that mediate nascent chain targeting and translocation.

  1991cites this paper
• Requirements for the membrane insertion of signal-anchor type proteins

  1991cites this paper
• Reconstitution of secretory protein translocation from detergent-solubilized rough microsomes.

  1991cites this paper
• Ribosome binding to the endoplasmic reticulum: a 180-kD protein identified by crosslinking to membrane-bound ribosomes is not required for ribosome binding activity

  1991cites this paper
• Probing the molecular environment of translocating polypeptide chains by cross-linking.

  1991cites this paper
• Vaccinia virus-mediated expression and identification of the human poliovirus receptor.

  1991cites this paper
• Changes in 7SL RNA conformation during the signal recognition particle cycle.

  1991cites this paper
• Characterization of the rough endoplasmic reticulum ribosome-binding activity

  1991cites this paper
• Assembly of the Alu domain of the signal recognition particle (SRP): dimerization of the two protein components is required for efficient binding to SRP RNA

  1990cites this paper