Cryocooling and radiation damage in macromolecular crystallography.

Advances in cryocrystallographic techniques for macromolecular crystallography have been intimately intertwined with efforts to reduce the deleterious effects of X-ray damage inflicted during the collection of diffraction data. A brief overview of cryomethods and their rationale is given. This is followed by a summary of our current limited understanding of radiation damage in cryocooled crystals, investigations aimed at minimizing its effects and finally some developments which actually utilize it both for phasing and to extend structural knowledge.

• Publication year

  2006

• Venue

  Acta Crystallographica Section D: Biological Crystallography

• Publication date

  Unknown publication date

• Fields of study

  Medicine, Materials Science, Physics

• Identifiers
  DOI 10.1107/S0907444905034207PMID 16369092
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The structure of cyclin E1/CDK2: implications for CDK2 activation and CDK2‐independent roles

  2005influential reference
• Phasing in the presence of radiation damage

  2005cited by this paper
• Three-dimensional numerical analysis of convection and conduction cooling of spherical biocrystals with localized heating from synchrotron X-ray beams.

  2005cited by this paper
• On the influence of the incident photon energy on the radiation damage in crystalline biological samples.

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• Pros and cons of cryocrystallography: should we also collect a room-temperature data set?

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  2005cited by this paper
• Will reduced radiation damage occur with very small crystals?

  2005cited by this paper
• Towards an understanding of radiation damage in cryocooled macromolecular crystals.

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  2004cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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• Cryocooling of macromolecular crystals: optimization methods.

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• Radiation damage of protein crystals at cryogenic temperatures between 40 K and 150 K.

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• Phasing in the presence of radiation damage.

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  2002cited by this paper
• Flash-cooling and annealing of protein crystals.

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• Unit-cell volume change as a metric of radiation damage in crystals of macromolecules.

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  2001cited by this paper
• Specific protein dynamics near the solvent glass transition assayed by radiation‐induced structural changes

  2001cited by this paper
• Solvent behaviour in flash-cooled protein crystals at cryogenic temperatures.

  2001cited by this paper
• X-ray beam/biomaterial thermal interactions in third-generation synchrotron sources.

  2001cited by this paper
• Potential for biomolecular imaging with femtosecond X-ray pulses

  2000cited by this paper
• Specific chemical and structural damage to proteins produced by synchrotron radiation.

  2000cited by this paper
• Characterization of conditions required for X-Ray diffraction experiments with protein microcrystals.

  2000cited by this paper
• The 'fingerprint' that X-rays can leave on structures.

  2000cited by this paper
• Structural changes in a cryo-cooled protein crystal owing to radiation damage.

  2000cited by this paper
• Primary radiation damage of protein crystals by an intense synchrotron X-ray beam.

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• Cryocrystallography with oil – an old idea revived

  1999influential reference
• Crystallization of hepatitis B virus core protein shells: determination of cryoprotectant conditions and preliminary X-ray characterization.

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• Cool data: quantity AND quality.

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• Use of cryoprotectants in combination with immiscible oils for flash cooling macromolecular crystals

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• A flash-annealing technique to improve diffraction limits and lower mosaicity in crystals of glycerol kinase.

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• Macromolecular Cryocrystallography: Cooling, Mounting, Storage and Transportation of Crystals

  1998cited by this paper
• Macromolecular crystal annealing: overcoming increased mosaicity associated with cryocrystallography.

  1998cited by this paper
• [13] Practical cryocrystallography.

  1997cited by this paper
• Raster3D: photorealistic molecular graphics.

  1997cited by this paper
• [28] Phase determination from multiwavelength anomalous diffraction measurements.

  1997cited by this paper
• [27] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods.

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• Flash-freezing causes a stress-induced modulation in a crystal structure of soybean lipoxygenase l3.

  1996cited by this paper
• Glycerol concentrations required for cryoprotection of 50 typical protein crystallization solutions

  1996cited by this paper
• Flash freezing of protein crystals: investigation of mosaic spread and diffraction limit with variation of cryoprotectant concentration

  1994cited by this paper
• Radiation damage in protein crystals at low temperature.

  1994cited by this paper
• Cryo-protection of protein crystals in intense x-ray beams

  1992cited by this paper
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  1992cited by this paper
• A program to produce both detailed and schematic plots of protein structures

  1991cited by this paper
• Mounting of crystals for macromolecular crystallography in a free-standing thin film

  1990cited by this paper
• Protein crystal perfection and the nature of radiation damage

  1988cited by this paper
• Evaluation of Single-Crystal X-ray Diffraction Data from a Position-Sensitive Detector

  1988cited by this paper
• Cryocrystallography of biological macromolecules: a generally applicable method.

  1988cited by this paper
• Structure and mobility of electron gain and loss centres in proteins

  1987cited by this paper
• The glass–liquid transition of hyperquenched water

  1987cited by this paper
• A nitrogen‐gas‐stream cryostat for general X‐ray diffraction studies

  1986cited by this paper
• Optimum X‐ray wavelength for protein crystallography

  1984cited by this paper
• REACTION MECHANISMS IN THE RADIOLYSIS OF PEPTIDES, POLYPEPTIDES AND PROTEINS II REACTIONS AT SIDE-CHAIN LOCI IN MODEL SYSTEMS

  1982cited by this paper
• Protein crystallography at sub-zero temperatures: cryo-protective mother liquors for protein crystals.

  1975cited by this paper
• Affinity chromatography of chymotrypsin(s).

  1974cited by this paper
• New method for extending the diffraction pattern from protein crystals and preventing their radiation damage

  1974cited by this paper
• Crystallographic studies on lactate dehydrogenase at-75 degrees C.

  1970cited by this paper
• Radiation Damage Synchrotron Radiation Parameters Affecting the X-ray Dose Absorbed by Macromolecular Crystals 2. Introduction

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• Evaluating the impact of X-ray damage on conformational heterogeneity in room-temperature (277 K) and cryo-cooled protein crystals.

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• Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source “Turkish DeLight”

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