Relationship of germinal centers in lymphoid tissue to immunologic memory. VI. Transfer of B cell memory with lymph node cells fractionated according to their receptors for peanut agglutinin.

We isolated germinal center B cells by exploiting their high affinity for peanut agglutinin (PNA). The PNA+ and PNA- B cells, fractionated by panning on PNA-coated petri dishes, were examined for their ability to transfer memory responses to irradiated recipients at various times after priming. With such fractionated B cells from lymph nodes taken at the peak of germinal center formation, the largest response was obtained in recipients of the PNA+ B cell population. At 4 to 5 wk after priming, and 10 days after challenge with an unrelated antigen, memory responses were approximately equal in recipients of PNA+ or PNA- B cells. At 14 wk after priming, memory responses were found only in recipients of the PNA- B cell population. Memory B cells from the spleen, taken from mice primed in the footpad 8 wk earlier, were also PNA-. Finally, we show that boosting with a TNP-conjugate in the footpad, 6 mo after priming in the same footpad, induced the reappearance of marked memory responsiveness in the PNA+ B cell fraction of the draining node.

• Publication year

  1983

• Venue

  Journal of Immunology

• Publication date

  1983-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.131.5.2254PMID 6605381
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Boosting with a TNP-conjugate in the footpad six months after priming induced a marked memory response in the PNA+ B cell fraction of the draining node.

  Confidence 0.94

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review
• At 14 weeks after priming, memory responses were detected only in recipients of the PNA- B cell population.

  Confidence 0.96

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review
• At 4 to 5 weeks after priming, and 10 days after challenge with an unrelated antigen, memory responses were approximately equal in recipients of PNA+ or PNA- B cells.

  Confidence 0.92

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review
• At the peak of germinal center formation, the largest transferred memory response came from the PNA+ B cell fraction isolated from lymph nodes.

  Confidence 0.97

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review

• germinal center b cells

  cell population

  B lymphocytes located in germinal centers and isolated from lymphoid tissue in this work.

  Aliases: GC B cells

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review
• irradiated recipients

  recipient, animal model

  Recipient animals given irradiation so that transferred lymph node cells could be tested for memory transfer.

  Aliases: irradiated mice

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review
• memory b cells

  cell population, immune component

  B cells responsible for transferring immunologic memory responses to recipient animals in the assay.

  Aliases: B cell memory

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review
• peanut agglutinin (pna)

  reagent, lectin

  A lectin used here as the basis for separating B-cell fractions by receptor binding.

  Aliases: PNA

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review
• pna+ b cells

  cell population

  The B-cell fraction that binds peanut agglutinin and was separated by panning on PNA-coated dishes.

  Aliases: PNA positive B cells, PNA-positive B cells

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review
• pna- b cells

  cell population

  The B-cell fraction that does not bind peanut agglutinin and was analyzed alongside the PNA+ fraction.

  Aliases: PNA negative B cells, PNA-negative B cells

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review
• tnp-conjugate

  antigen conjugate, reagent

  A trinitrophenyl-containing antigen conjugate used for boosting primed mice in the footpad.

  Aliases: TNP conjugate

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) review

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