Population heterogeneity in microbial bioprocesses: origin, analysis, mechanisms, and future perspectives

No abstract is available for this paper.

• Publication year

  2018

• Venue

  Bioprocess and biosystems engineering (Print)

• Publication date

  2018-03-14

• Fields of study

  Biology, Medicine, Engineering, Environmental Science

• Identifiers
  DOI 10.1007/s00449-018-1922-3PMID 29541890
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Metabolic profile of 1,5‐diaminopentane producing Corynebacterium glutamicum under scale‐down conditions: Blueprint for robustness to bioreactor inhomogeneities

  2017cited by this paper
• Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity.

  2017influential reference
• Heterogeneity in Pure Microbial Systems: Experimental Measurements and Modeling

  2017cited by this paper
• Increased lignocellulosic inhibitor tolerance of Saccharomyces cerevisiae cell populations in early stationary phase

  2017cited by this paper
• Lactic acid production from submerged fermentation of broken rice using undefined mixed culture

  2017cited by this paper
• Tools for the determination of population heterogeneity caused by inhomogeneous cultivation conditions.

  2017influential reference
• A novel model‐based control strategy for aerobic filamentous fungal fed‐batch fermentation processes

  2017cited by this paper
• Microbial amensalism in Lactobacillus casei and Pseudomonas taetrolens mixed culture

  2017cited by this paper
• Mass Cytometry for Detection of Silver at the Bacterial Single Cell Level

  2017cited by this paper
• Application of flow cytometry for monitoring the production of poly(3‐hydroxybutyrate) by Halomonas boliviensis

  2017cited by this paper
• Flow Cytometric Single-Cell Identification of Populations in Synthetic Bacterial Communities

  2017cited by this paper
• History of adaptation determines short‐term shifts in performance and community structure of hydrogen‐producing microbial communities degrading wheat straw

  2017cited by this paper
• Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775

  2017cited by this paper
• Taking control over microbial populations: Current approaches for exploiting biological noise in bioprocesses

  2017cited by this paper
• A 9‐pool metabolic structured kinetic model describing days to seconds dynamics of growth and product formation by Penicillium chrysogenum

  2017cited by this paper
• Establishment of a fully automated microtiter plate‐based system for suspension cell culture and its application for enhanced process optimization

  2017cited by this paper
• Self-organization of bacterial communities against environmental pH variation: Controlled chemotactic motility arranges cell population structures in biofilms

  2017cited by this paper
• Transcriptional response of Escherichia coli to ammonia and glucose fluctuations

  2017cited by this paper
• Metastable coexistence of multiple genotypes in a constant environment with a single resource through fixed settings of a multiplication-survival trade-off.

  2016cited by this paper
• Characterizing bubble column bioreactor performance using computational fluid dynamics

  2016cited by this paper
• Mass Cytometry: Single Cells, Many Features.

  2016cited by this paper
• Noise-driven growth rate gain in clonal cellular populations

  2016cited by this paper
• Intracellular pH Response to Weak Acid Stress in Individual Vegetative Bacillus subtilis Cells

  2016cited by this paper
• Use of noise in gene expression as an experimental parameter to test phenotypic effects

  2016cited by this paper
• Identifying Metabolic Subpopulations from Population Level Mass Spectrometry

  2016cited by this paper
• Genetically encoded sensors enable real-time observation of metabolite production

  2016cited by this paper
• Online flow cytometry reveals microbial dynamics influenced by concurrent natural and operational events in groundwater used for drinking water treatment

  2016cited by this paper
• Growth resumption from stationary phase reveals memory in Escherichia coli cultures

  2016influential reference
• A Toolbox of Genetically Encoded FRET-Based Biosensors for Rapid l-Lysine Analysis

  2016cited by this paper
• Mixed consortia in bioprocesses: role of microbial interactions

  2016influential reference
• Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation

  2016influential reference
• CFD of mixing of multi‐phase flow in a bioreactor using population balance model

  2016cited by this paper
• Cellular Heterogeneity: Benefits Besides Bet-Hedging.

  2016influential reference
• Reevaluating multicolor flow cytometry to assess microbial viability

  2016cited by this paper
• Cell‐to‐cell heterogeneity emerges as consequence of metabolic cooperation in a synthetic yeast community

  2016cited by this paper
• Evaluation of viability, metabolic activity and spore quantity in clostridial cultures during ABE fermentation.

  2016cited by this paper
• Engineering E. coli for large-scale production - Strategies considering ATP expenses and transcriptional responses.

  2016influential reference
• Light-Controlled Cell Factories: Employing Photocaged Isopropyl-β-d-Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum

  2016cited by this paper
• Screening of an Escherichia coli promoter library for a phenylalanine biosensor

  2016cited by this paper
• Modulation of heterologous expression from PBAD promoter in Escherichia coli production strains.

  2016cited by this paper
• A flexible well-mixed milliliter-scale reactor with high oxygen transfer rate for microbial cultivations

  2016influential reference
• Defining heterogeneity within bacterial populations via single cell approaches

  2016cited by this paper
• Noise and Epigenetic Inheritance of Single-Cell Division Times Influence Population Fitness.

  2016cited by this paper
• Frequency‐dependent selection: a diversifying force in microbial populations

  2016cited by this paper
• Experimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids.

  2016cited by this paper
• Application of flow cytometry analysis to elucidate the impact of scale-down conditions in Escherichia coli cultivations

  2016cited by this paper
• Phenotypic Variability in Synthetic Biology Applications: Dealing with Noise in Microbial Gene Expression

  2016cited by this paper
• Fluorescent Reporter Libraries as Useful Tools for Optimizing Microbial Cell Factories: A Review of the Current Methods and Applications

  2015influential reference
• Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide

  2015cited by this paper
• Transcription factor-based biosensors in biotechnology: current state and future prospects

  2015cited by this paper
• Rate control in yeast protein synthesis at the population and single-cell levels.

  2015cited by this paper
• Response of Corynebacterium glutamicum exposed to oscillating cultivation conditions in a two‐ and a novel three‐compartment scale‐down bioreactor

  2015cited by this paper
• Mutational heterogeneity: A key ingredient of bet‐hedging and evolutionary divergence?

  2015cited by this paper
• Surviving the heat: heterogeneity of response in Saccharomyces cerevisiae provides insight into thermal damage to the membrane

  2015cited by this paper
• Technical bias of microcultivation environments on single-cell physiology.

  2015cited by this paper
• Phenotypic variability in bioprocessing conditions can be tracked on the basis of on-line flow cytometry and fits to a scaling law.

  2015influential reference
• Non-Invasive Microbial Metabolic Activity Sensing at Single Cell Level by Perfusion of Calcein Acetoxymethyl Ester

  2015cited by this paper
• Experimental and in silico investigation of population heterogeneity in continuous Sachharomyces cerevisiae scale‐down fermentation in a two‐compartment setup

  2015cited by this paper
• Connecting growth with gene expression: of noise and numbers.

  2015influential reference
• Noise Expands the Response Range of the Bacillus subtilis Competence Circuit

  2015cited by this paper
• FRET-based genetically-encoded sensors for quantitative monitoring of metabolites

  2015cited by this paper
• Classification of phenotypic subpopulations in isogenic bacterial cultures by triple promoter probing at single cell level.

  2015cited by this paper
• Assessment of morphological changes of Clostridium acetobutylicum by flow cytometry during acetone/butanol/ethanol extractive fermentation

  2015cited by this paper
• A functional perspective on phenotypic heterogeneity in microorganisms

  2015influential reference
• Novel screening methods--biosensors.

  2015cited by this paper
• Integration of microbial kinetics and fluid dynamics toward model‐driven scale‐up of industrial bioprocesses

  2015influential reference
• Genetically-encoded biosensors for monitoring cellular stress in bioprocessing.

  2015cited by this paper
• Non-random distribution of macromolecules as driving forces for phenotypic variation.

  2015cited by this paper
• Spatiotemporal microbial single‐cell analysis using a high‐throughput microfluidics cultivation platform

  2015influential reference
• Better together: engineering and application of microbial symbioses.

  2015influential reference
• Microbial individuality: how single-cell heterogeneity enables population level strategies.

  2015influential reference
• Persister Heterogeneity Arising from a Single Metabolic Stress.

  2015cited by this paper
• The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress

  2015cited by this paper
• Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains

  2015cited by this paper
• Single Cell Protein Analysis

  2015cited by this paper
• Exploring the potential of the glycerol-3-phosphate dehydrogenase 2 (GPD2) promoter for recombinant gene expression in Saccharomyces cerevisiae

  2015cited by this paper
• Adaptation to sustained nitrogen starvation by Escherichia coli requires the eukaryote-like serine/threonine kinase YeaG

  2015cited by this paper
• Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes.

  2015influential reference
• Microbial bet-hedging: the power of being different.

  2015influential reference
• Measuring gene expression in single bacterial cells: recent advances in methods and micro-devices

  2015influential reference
• Construction of malate-sensing Escherichia coli by introduction of a novel chimeric two-component system

  2015cited by this paper
• Individuality, phenotypic differentiation, dormancy and ‘persistence’ in culturable bacterial systems: commonalities shared by environmental, laboratory, and clinical microbiology

  2015cited by this paper
• Noise in gene expression is coupled to growth rate

  2015influential reference
• Methodologies for Scale-down of Microbial Bioprocesses

  2015cited by this paper
• Co-culture systems and technologies: taking synthetic biology to the next level

  2014cited by this paper
• Metabolic variability in bioprocessing: implications of microbial phenotypic heterogeneity.

  2014influential reference
• Subpopulation-proteomics reveal growth rate, but not cell cycling, as a major impact on protein composition in Pseudomonas putida KT2440

  2014cited by this paper
• Population heterogeneity and dynamics in starter culture and lag phase adaptation of the spoilage yeast Zygosaccharomyces bailii to weak acid preservatives

  2014cited by this paper
• Age-correlated stress resistance improves fitness of yeast: support from agent-based simulations

  2014influential reference
• Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level.

  2014influential reference
• NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level

  2014cited by this paper
• Massive Diversification in Aging Colonies of Escherichia coli

  2014cited by this paper
• Modeling Bacterial Population Growth from Stochastic Single-Cell Dynamics

  2014cited by this paper
• Scale-down effect on the extracellular proteome of Escherichia coli: correlation with membrane permeability and modulation according to substrate heterogeneities

  2014cited by this paper
• Recent advancements in optofluidics-based single-cell analysis: optical on-chip cellular manipulation, treatment, and property detection.

  2014cited by this paper
• Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry

  2014cited by this paper
• Flow cytometric analysis of E. coli on agar plates: implications for recombinant protein production

  2014cited by this paper
• Stochasticity of metabolism and growth at the single-cell level

  2014cited by this paper
• Process inhomogeneity leads to rapid side product turnover in cultivation of Corynebacterium glutamicum

  2014cited by this paper
• Single Cell Kinetics of Phenotypic Switching in the Arabinose Utilization System of E. coli

  2014cited by this paper

• Spatially-resolved optical monitoring of bioreactor cell growth

  2026cites this paper
• Analysis of autofluorescence using full-spectrum cytometry to discriminate and monitor microalgae and bacteria

  2026cites this paper
• Biosurfactant-Producing Bacteria Isolated from a Microbial Consortium Previously Subjected to Adaptive Laboratory Evolution in Oily Sludge.

  2026cites this paper
• Considerations of Bacterial Robustness and Stability to Improve Bioprocess Design

  2026cites this paper
• The development of Burkholderia bacteria as heterologous hosts

  2025cites this paper
• Monitoring of the Single-Cell Behavior of an Escherichia coli Reporter Strain Producing L-phenylalanine in a Scale-Down Bioreactor by Automated Real-Time Flow Cytometry

  2025cites this paper
• Exchange of the l-cysteine exporter after in-vivo metabolic control analysis improved the l-cysteine production process with engineered Escherichia coli

  2025cites this paper
• Lagrangian Sensor Particles for detecting hydrodynamic heterogeneities in industrial bioreactors: Experimental analysis and Lattice-Boltzmann simulations

  2025cites this paper
• Enhancing Vitamin B12 Production in Engineered Escherichia coli through Cofactor Engineering and Fermentation Media Optimization.

  2025cites this paper
• A double-staining automated flow cytometry method for real-time monitoring of bacteria in continuous bioreactors

  2025cites this paper
• Investigation on Quality Discrepancies of Soy Sauce Aroma Baijiu: Flavor Chemical Characteristics in Single-Source and Cross-Source Samples

  2025cites this paper
• A co-feeding strategy of formate and H2 for methanogens – Enhancing growth parameters and methane production

  2025cites this paper
• Wireless and passive pressure detection using magneto-mechanical resonances in process engineering

  2025cites this paper
• Cellular variability as a driver for bioprocess innovation and optimization.

  2025cites this paper
• Pilot-Scale Oxygen-Balanced Mixotrophic Cultivation of Galdieria sulphuraria

  2025cites this paper
• Identification and monitoring of cell heterogeneity from plasmid recombination during limonene production

  2025cites this paper
• Kinetic modeling of terpenoid production in E. coli: insights into subpopulation emergence and process optimization

  2025cites this paper
• Mathematical Modeling of Escherichia coli and Lactobacillus acidophilus Growth Based on Experimental Mixed Batch Cultivation

  2025cites this paper
• Resolving misconceptions and constraints in growth-coupled bioproduction.

  2025cites this paper
• Holistic Bioprocess Development Across Scales Using Multi-Fidelity Batch Bayesian Optimization

  2025cites this paper
• A Fitness–Entropy Compensation effect set the trade-off between growth and gene expression in cell populations

  2025cites this paper
• Single cell technologies for monitoring protein secretion heterogeneity.

  2024cites this paper
• Mixed Culture Cultivation in Microbial Bioprocesses.

  2024cites this paper
• Quantifying microbial robustness in dynamic environments using microfluidic single-cell cultivation

  2024cites this paper
• Real Case Study of 600 m3 Bubble Column Fermentations: Spatially Resolved Simulations Unveil Optimization Potentials for l‐Phenylalanine Production With Escherichia coli

  2024cites this paper
• Giving the cells what they need when they need it: Biosensor‐based feeding control

  2024cites this paper
• Exploiting phenotypic heterogeneity to improve production of glutathione by yeast

  2024cites this paper
• Influence of Varying Pre-Culture Conditions on the Level of Population Heterogeneity in Batch Cultures with an Escherichia coli Triple Reporter Strain

  2023cites this paper
• Four ways of implementing robustness quantification in strain characterisation

  2023cites this paper
• Deep in situ microscopy for real-time analysis of mammalian cell populations in bioreactors

  2023cites this paper
• Monitoring Phenotype Heterogeneity at the Single-Cell Level within Bacillus Populations Producing Poly-3-hydroxybutyrate by Label-Free Super-resolution Infrared Imaging

  2023cites this paper
• Impact of mixing insufficiencies on L-phenylalanine production with an Escherichia coli reporter strain in a novel two-compartment bioreactor

  2023cites this paper
• PHB production from food waste hydrolysates by Halomonas bluephagenesis Harboring PHB operon linked with an essential gene.

  2023cites this paper
• Comparison of plasmid stabilization systems during heterologous isopropanol production in fed-batch bioreactor.

  2023cites this paper
• Harnessing microfluidic technology for bacterial single-cell analysis in mammals

  2023cites this paper
• Dynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica

  2023cites this paper
• Analyzing and understanding the robustness of bioprocesses.

  2023cites this paper
• Modeling free surface gas transfer in agitated lab-scale bioreactors

  2023cites this paper
• Versteckte Vielfalt: Diversität von Zellen im industriellen Bioprozess

  2023influential citation
• Modelling of industrial-scale bioreactors using the particle lifeline approach

  2023cites this paper
• Longitudinal Single‐Cell Imaging of Engineered Strains with Stimulated Raman Scattering to Characterize Heterogeneity in Fatty Acid Production

  2023cites this paper
• Modeling multiphase fluid flow, mass transfer, and chemical reactions in bioreactors using large‐eddy simulation

  2022cites this paper
• Real-Time Monitoring of the Yeast Intracellular State During Bioprocesses With a Toolbox of Biosensors

  2022cites this paper
• Study of plasmid-based expression level heterogeneity under plasmid-curing like conditions in Cupriavidus necator.

  2022cites this paper
• The SiLA 2 Manager for rapid device integration and workflow automation

  2022cites this paper
• Robustness: linking strain design to viable bioprocesses.

  2022cites this paper
• Application of an Escherichia coli triple reporter strain for at‐line monitoring of single‐cell physiology during L‐phenylalanine production

  2022cites this paper
• Monitoring Intracellular Metabolite Dynamics in Saccharomyces cerevisiae during Industrially Relevant Famine Stimuli

  2022cites this paper
• Quantifying heterologous gene expression during ectopic MazF production in Escherichia coli

  2022cites this paper
• Digital models in biotechnology: Towards multi-scale integration and implementation.

  2022cites this paper
• Control of phenotypic diversification based on serial cultivations on different carbon sources leads to improved bacterial xylanase production

  2022cites this paper
• Co-expression of an isopropanol synthetic operon and eGFP to monitor the robustness of Cupriavidus necator during isopropanol production.

  2022cites this paper
• Optimization and Scale-Up of Fermentation Processes Driven by Models

  2022cites this paper
• Control of parallelized bioreactors I: dynamic scheduling software for efficient bioprocess management in high-throughput systems

  2022cites this paper
• Bioprocess Automation with Robotics: Streamlining Microbiology for Biotech Industry

  2022cites this paper
• Advances in automated real‐time flow cytometry for monitoring of bioreactor processes

  2021influential citation
• Гетерогенность в изогенных популяциях бактерий и современные технологии клеточного фенотипирования

  2021cites this paper
• The ecology of wine fermentation: a model for the study of complex microbial ecosystems

  2021cites this paper
• Biodegradation of waste cooking oil and simultaneous production of rhamnolipid biosurfactant by Pseudomonas aeruginosa P7815 in batch and fed-batch bioreactor

  2021cites this paper
• Heterogeneity in isogenic bacteria populations and modern technologies of cell phenotyping

  2021cites this paper
• Ein Wechselbad für Mikroben: Wachsen unter dynamischen Umweltbedingungen

  2021cites this paper
• Comparing cell population balance model simulation through Gaussian processes and discretisation

  2021cites this paper
• Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope

  2021cites this paper
• Characterization of cell cycle and apoptosis in Chinese hamster ovary cell culture using flow cytometry for bioprocess monitoring

  2021cites this paper
• Recent advances in fed-batch microscale bioreactor design.

  2021cites this paper
• Longitudinal single-cell chemical imaging of engineered strains reveals heterogeneity in fatty acid production

  2021cites this paper
• Fluctuations in glucose availability prevent global proteome changes and physiological transition during prolonged chemostat cultivations of Saccharomyces cerevisiae

  2020cites this paper
• Biotechnology and Bioengineering

  2020cites this paper
• Fluctuations in glucose availability prevent global proteome changes and physiological transition during prolonged chemostat cultivations of S. cerevisiae

  2020cites this paper
• Use of qPCR for the analysis of population heterogeneity and dynamics during Lactobacillus delbrueckii spp. bulgaricus batch culture

  2020cites this paper
• Understanding gradients in industrial bioreactors.

  2020cites this paper
• Advanced metabolic engineering strategies for the development of sustainable microbial processes

  2020cites this paper
• Developing a Computational Framework To Advance Bioprocess Scale-Up.

  2020cites this paper
• Development of Novel Bioreactor Control Systems Based on Smart Sensors and Actuators

  2020cites this paper
• Development and characterization of Escherichia coli triple reporter strains for investigation of population heterogeneity in bioprocesses

  2020cites this paper
• Effect of the oxygen transfer rate on oxygen-limited production of plasmid DNA by Escherichia coli

  2019cites this paper
• Controlling and exploiting cell-to-cell variation in metabolic engineering.

  2019cites this paper
• Linear scanning ATR-FTIR for mapping and high throughput studies of bacterial biofilms in microfluidic channels

  2019cites this paper
• Environmental drivers of metabolic heterogeneity in clonal microbial populations.

  2019cites this paper
• Coupled metabolic‐hydrodynamic modeling enabling rational scale‐up of industrial bioprocesses

  2019cites this paper
• Investigating the Relationship and Potential Interactions of CD108131 and SGCE

  2019cites this paper
• A Physiologically Structured Equation to Consider Quota Heterogeneity in the Droop Model

  2019cites this paper
• The effect of acetate on population heterogeneity in different cellular characteristics of Escherichia coli in aerobic batch cultures

  2019influential citation
• Biphasic whole-cell production of geranyl glucoside by recombinant Escherichia coli

  2019cites this paper
• Quantitative Flow Cytometry to Understand Population Heterogeneity in Response to Changes in Substrate Availability in Escherichia coli and Saccharomyces cerevisiae Chemostats

  2019influential citation
• Biotechnology and Bioengineering

  2019cites this paper
• Engineering E. coli for improved microaerobic pDNA production

  2019cites this paper
• Linear Scanning ATR-FTIR for Chemical Mapping and High-Throughput Studies of Pseudomonas sp. Biofilms in Microfluidic Channels.

  2018cites this paper
• Compatibility engineering of synthetic metabolic pathways in microbial cell factories

  year unknowncites this paper