Molecular cloning of murine pig-a, a gene for GPI-anchor biosynthesis, and demonstration of interspecies conservation of its structure, function, and genetic locus.

No abstract is available for this paper.

• Publication year

  1994

• Venue

  Genomics

• Publication date

  1994-10-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1006/GENO.1994.1544PMID 7851884
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Paroxysmal nocturnal haemoglobinuria (PNH) is caused by somatic mutations in the PIG‐A gene.

  1994cited by this paper
• Abnormalities of PIG-A transcripts in granulocytes from patients with paroxysmal nocturnal hemoglobinuria.

  1994cited by this paper
• Characterization of genomic PIG-A gene: a gene for glycosylphosphatidylinositol-anchor biosynthesis and paroxysmal nocturnal hemoglobinuria.

  1994cited by this paper
• Deficiency of the GPI anchor caused by a somatic mutation of the PIG-A gene in paroxysmal nocturnal hemoglobinuria.

  1993cited by this paper
• The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis.

  1993cited by this paper
• Early lipid intermediates in glycosyl-phosphatidylinositol anchor assembly are synthesized in the ER and located in the cytoplasmic leaflet of the ER membrane bilayer

  1993cited by this paper
• The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes.

  1993cited by this paper
• Sucrose synthase genes in barley

  1993cited by this paper
• Deficient biosynthesis of N-acetylglucosaminyl-phosphatidylinositol, the first intermediate of glycosyl phosphatidylinositol anchor biosynthesis, in cell lines established from patients with paroxysmal nocturnal hemoglobinuria

  1993cited by this paper
• Post-translational modifications of the Dictyostelium discoideum glycoprotein PsA. Glycosylphosphatidylinositol membrane anchor and composition of O-linked oligosaccharides.

  1993cited by this paper
• Specific defect in N-acetylglucosamine incorporation in the biosynthesis of the glycosylphosphatidylinositol anchor in cloned cell lines from patients with paroxysmal nocturnal hemoglobinuria.

  1993cited by this paper
• The structure and biosynthesis of glycosyl phosphatidylinositol protein anchors.

  1993cited by this paper
• Affected paroxysmal nocturnal hemoglobinuria T lymphocytes harbor a common defect in assembly of N-acetyl-D-glucosamine inositol phospholipid corresponding to that in class A Thy-1- murine lymphoma mutants.

  1992cited by this paper
• Defective glycosylphosphatidylinositol anchor synthesis in paroxysmal nocturnal hemoglobinuria granulocytes.

  1992cited by this paper
• How to make a glycoinositol phospholipid anchor.

  1992cited by this paper
• Induction of irreversible glomerulosclerosis in the rat by repeated injections of a monoclonal anti-Thy-1.1 antibody.

  1992cited by this paper
• Deficient surface expression of glycosylphosphatidylinositol-anchored proteins in B cell lines established from patients with paroxysmal nocturnal hemoglobinuria.

  1992cited by this paper
• Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12

  1992cited by this paper
• Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core

  1992cited by this paper
• Synthesis of mannosylglucosaminylinositol phospholipids in normal but not paroxysmal nocturnal hemoglobinuria cells.

  1992cited by this paper
• Derivation and characterization of glycoinositol-phospholipid anchor-defective human K562 cell clones.

  1992cited by this paper
• Identification of defects in glycosylphosphatidylinositol anchor biosynthesis in the Thy-1 expression mutants.

  1991cited by this paper
• Defective glycosyl phosphatidylinositol biosynthesis in extracts of three Thy-1 negative lymphoma cell mutants.

  1991cited by this paper
• A gene coding for 3-deoxy-D-manno-octulosonic-acid transferase in Escherichia coli. Identification, mapping, cloning, and sequencing.

  1991cited by this paper
• The Saccharomyces cerevisiae SPT14 gene is essential for normal expression of the yeast transposon, Ty, as well as for expression of the HIS4 gene and several genes in the mating pathway

  1991cited by this paper
• Cloning, characterization, and DNA sequence of the rfaLK region for lipopolysaccharide synthesis in Salmonella typhimurium LT2

  1991cited by this paper
• Two glycogen synthase isoforms in Saccharomyces cerevisiae are coded by distinct genes that are differentially controlled.

  1991cited by this paper
• Emerging functional roles for the glycosyl-phosphatidylinositol membrane protein anchor

  1990cited by this paper
• Pyruvate dehydrogenase E1 alpha subunit genes in the mouse: mapping and comparison with human homologs.

  1990cited by this paper
• Structure, biosynthesis, and function of glycosylphosphatidylinositols.

  1990cited by this paper
• The glycosyl-phosphatidylinositol anchor of membrane proteins.

  1989cited by this paper
• Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1 alpha.

  1989cited by this paper
• Paroxysmal nocturnal hemoglobinuria: the biochemical defects and the clinical syndrome.

  1989cited by this paper
• X-chromosome localization of the functional gene for the E1 alpha subunit of the human pyruvate dehydrogenase complex.

  1989cited by this paper
• Glycosyl-phosphatidylinositol moiety that anchors Trypanosoma brucei variant surface glycoprotein to the membrane.

  1988cited by this paper
• Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

  1988cited by this paper
• Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells

  1988cited by this paper
• Complete structure of the glycosyl phosphatidylinositol membrane anchor of rat brain Thy-1 glycoprotein

  1988cited by this paper
• Biosynthesis and function of LFA-3 in human mutant cells deficient in phosphatidylinositol-anchored proteins.

  1988cited by this paper
• Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structures.

  1988cited by this paper
• Structural characterization of the glycoinositol phospholipid membrane anchor of human erythrocyte acetylcholinesterase by fast atom bombardment mass spectrometry.

  1988cited by this paper
• Somatic genetic analysis of the expression of cell surface molecules.

  1988cited by this paper
• An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.

  1987cited by this paper
• High-efficiency transformation of mammalian cells by plasmid DNA.

  1987cited by this paper
• Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

  1987cited by this paper
• A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.

  1983cited by this paper

• Erythrocyte-Based Pig-a Gene Mutation Assay.

  2026cites this paper
• Systems for Genetic Assessment of the Impact of Environmental Factors

  2024cites this paper
• PIG-A gene mutation as a mutagenicity biomarker among coke oven workers.

  2023cites this paper
• The PIG‐A gene mutation assay in human biomonitoring and disease

  2023cites this paper
• Insights Into the Emergence of Paroxysmal Nocturnal Hemoglobinuria

  2022cites this paper
• Toxicity Testing to Understand the Mutagenicity of Pharmaceutical Impurities

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• Dose-response genotoxicity of triclosan in mice: an estimate of acceptable daily intake based on organ toxicity.

  2021cites this paper
• PIG-A gene mutation as a genotoxicity biomaker in polycyclic aromatic hydrocarbon-exposed barbecue workers

  2021cites this paper
• Absence of hydroxyurea‐induced mutational effects supports higher utilisation for the treatment of sickle cell anaemia

  2021cites this paper
• Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency

  2021cites this paper
• Pig‐a gene mutations in bone marrow granulocytes of procarbazine‐treated F344 rats

  2021cites this paper
• Standard protocol for the PIGRET assay, a high-throughput reticulocyte Pig-a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society

  2021cites this paper
• PIG‐A gene mutation as a genotoxicity biomarker in human population studies: An investigation in lead‐exposed workers

  2020cites this paper
• Evaluation of PIG‐A‐mutated granulocytes and ex‐vivo binucleated micronucleated lymphocytes frequencies after breast cancer radiotherapy in humans

  2020cites this paper
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  2020cites this paper
• Pig-a gene mutation assay study design: critical assessment of 3- versus 28-day repeat-dose treatment schedules.

  2020cites this paper
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  2020cites this paper
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  2019cites this paper
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  2019cites this paper
• Erythrocyte-Based Pig-a Gene Mutation Assay.

  2019cites this paper
• Assessment of the Pig‐a, micronucleus, and comet assay endpoints in rats treated by acute or repeated dosing protocols with procarbazine hydrochloride and ethyl carbamate

  2018cites this paper
• Mutagenicity Testing: Regulatory Guidelines and Current Needs

  2018cites this paper
• The development of an in vitro Pig-a assay in L5178Y cells

  2018cites this paper
• [The PIG-A gene as a new biomarker of mutagenesis: proof of concept and technical specifications].

  2017cites this paper
• The Pig-a Gene Mutation Assay in Mice and Human Cells: A Review.

  2017cites this paper
• Salivary DNA Methylation Profiling: Aspects to Consider for Biomarker Identification

  2017cites this paper
• Development of an in vitro PIG-A gene mutation assay in human cells

  2017cites this paper
• Evaluation of red blood cell Pig-a assay and PIGRET assay in rats using chlorambucil.

  2016cites this paper
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  2016cites this paper
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  2016cites this paper
• The Pig-a Endogenous Gene Mutation Assay

  2016cites this paper
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  2016cites this paper
• Evaluation of single-dose RBC Pig-a and PIGRET assays in detecting the mutagenicity of thiotepa in rats.

  2016cites this paper
• Evaluation of the RBC Pig-a and PIGRET assays using single doses of hydroxyurea and melphalan in rats.

  2016cites this paper
• Differential genotoxicity of acrylamide in the micronucleus and Pig-a gene mutation assays in F344 rats and B6C3F1 mice.

  2016cites this paper
• Continued progress in developing the Pig-a gene mutation assay.

  2015cites this paper
• Early Frameshift Mutation in PIGA Identified in a Large XLID Family Without Neonatal Lethality

  2014cites this paper
• The In Vivo Pig-a Gene Mutation Assay

  2014cites this paper
• Persistence of red blood cells with Pig-a mutation in p53 knockout mice exposed to X-irradiation.

  2014cites this paper
• Pig-a Gene Mutation and Micronucleus Induction in Rat Peripheral Blood by Methyl Methanesulfonate

  2014cites this paper
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  2014cites this paper
• Erythrocyte-based Pig-a gene mutation assay.

  2013cites this paper
• Interlaboratory trial of the rat Pig-a mutation assay using an erythroid marker HIS49 antibody.

  2013cites this paper
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  2013cites this paper
• Effective use of the Pig-a gene mutation assay for mutagenicity screening: measuring CD59-deficient red blood cells in rats treated with genotoxic chemicals.

  2012cites this paper
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  2012cites this paper
• Genotoxicity of TiO(2) anatase nanoparticles in B6C3F1 male mice evaluated using Pig-a and flow cytometric micronucleus assays.

  2012cites this paper
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  2011cites this paper
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  2011cites this paper
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  2011cites this paper
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  2011cites this paper
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  2010cites this paper
• Hémoglobinurie paroxystique nocturne

  2010cites this paper
• [Paroxysmal nocturnal hemoglobinuria].

  2010cites this paper
• Genetic Toxicity: In Vitro Approaches for Medicinal Chemists: In Vitro Approaches for Medicinal Chemists

  2010cites this paper
• Accumulation and persistence of Pig-A mutant peripheral red blood cells following treatment of rats with single and split doses of N-ethyl-N-nitrosourea.

  2009cites this paper
• Convergent extension movements in growth plate chondrocytes require gpi-anchored cell surface proteins

  2009cites this paper
• Trypanosome glycosylphosphatidylinositol biosynthesis.

  2009cites this paper
• Unique motifs identify PIG-A proteins from glycosyltransferases of the GT4 family

  2008cites this paper
• In vivo mutation assay based on the endogenous Pig‐a locus

  2008cites this paper
• Hemoglobinuria paroxística nocturna

  2008cites this paper
• [Paroxysmal nocturnal hemoglobinuria].

  2008cites this paper
• Development of an in vivo gene mutation assay using the endogenous Pig‐A gene: II. Selection of Pig‐A mutant rat spleen T‐cells with proaerolysin and sequencing Pig‐A cDNA from the mutants

  2008cites this paper
• Development of an in vivo gene mutation assay using the endogenous Pig‐A gene: I. Flow cytometric detection of CD59‐negative peripheral red blood cells and CD48‐negative spleen T‐cells from the rat

  2008influential citation
• Erythrocyte-based Pig-a gene mutation assay: demonstration of cross-species potential.

  2008cites this paper
• [Paroxysmal nocturnal hemoglobinuria].

  2007cites this paper
• Gpi19, the Saccharomyces cerevisiae Homologue of Mammalian PIG-P, Is a Subunit of the Initial Enzyme for Glycosylphosphatidylinositol Anchor Biosynthesis

  2005cites this paper
• The Journal of Toxicological Sciences

  2005cites this paper
• Genome-wide phenotype analysis in ES cells by regulated disruption of Bloom's syndrome gene

  2004cites this paper
• Mice with Blood Cells Deficient in GPI-linked Proteins — The Generation of a Murine Model to Investigate the Pathogenesis and Pathophysiology of Paroxysmal Nocturnal Hemoglobinuria (PNH)

  2003cites this paper
• Glycosylphosphatidylinositol‐linked proteins are required for maintenance of a normal peripheral lymphoid compartment but not for lymphocyte development

  2002cites this paper
• GATA1-Cre mediatesPigagene inactivation in the erythroid/megakaryocytic lineage and leads to circulating red cells with a partial deficiency in glycosyl phosphatidylinositol–linked proteins (paroxysmal nocturnal hemoglobinuria type II cells)

  2001cites this paper
• Functional analysis of the yeast "Saccharomyces cerevisiae" Gpi8 protein and characterization of the purified GPI-transamidase complex

  2001cites this paper
• The human GPI1 gene is required for efficient glycosylphosphatidylinositol biosynthesis.

  2001cites this paper
• GATA 1-Cre mediates Piga gene inactivation in the erythroid / megakaryocytic lineage and leads to circulating red cells with a partial deficiency in glycosyl phosphatidylinositol – linked proteins ( paroxysmal nocturnal hemoglobinuria type II cells )

  2001cites this paper
• Efficient chromosomal transposition of a Tc1/mariner- like transposon Sleeping Beauty in mice

  2001cites this paper
• Ynl038wp (Gpi15p) is the Saccharomyces cerevisiae homologue of human Pig‐Hp and participates in the first step in glycosylphosphatidylinositol assembly

  2001cites this paper
• GATA1-Cre mediates Piga gene inactivation in the erythroid/megakaryocytic lineage and leads to circulating red cells with a partial deficiency in glycosyl phosphatidylinositol-linked proteins (paroxysmal nocturnal hemoglobinuria type II cells).

  2001cites this paper
• Glycosyl phosphatidylinositol (GPI)-anchored molecules and the pathogenesis of paroxysmal nocturnal hemoglobinuria.

  2000cites this paper
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  2000cites this paper
• Photoaffinity labelling with P3-(4-azidoanilido)uridine 5'-triphosphate identifies gpi3p as the UDP-GlcNAc-binding subunit of the enzyme that catalyses formation of GlcNAc-phosphatidylinositol, the first glycolipid intermediate in glycosylphosphatidylinositol synthesis.

  2000cites this paper
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  2000cites this paper
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  2000influential citation
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  2000cites this paper
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  1999cites this paper
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  1999cites this paper
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  1999cites this paper
• Biosynthesis of Glycosylphosphatidylinositols in Mammals and Unicellular Microbes

  1999cites this paper
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  1999cites this paper
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  1999cites this paper
• Paroxysmal nocturnal hemoglobinuria: molecular pathogenesis and molecular therapeutic approaches.

  1998cites this paper
• Functional competence of T cells in the absence of glycosylphosphatidylinositol‐anchored proteins caused by T cell‐specific disruption of the Pig‐agene

  1998cites this paper
• The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG‐A, PIG‐H, PIG‐C and GPI1

  1998cites this paper
• Somatic mutation and clonal selection in the pathogenesis and in the control of paroxysmal nocturnal hemoglobinuria.

  1998cites this paper
• PIG‐A gene mutations in Four Taiwanese Patients with Paroxysmal Nocturnal Haemoglobinuria Following Aplastic Anaemia

  1997cites this paper
• Recent advances in paroxysmal nocturnal hemoglobinuria. From the biology to the clinic.

  1997cites this paper
• A patient with paroxysmal nocturnal hemoglobinuria bearing four independent PIG-A mutant clones.

  1997cites this paper
• Structural and functional analysis of the Pig-a protein that is mutated in paroxysmal nocturnal hemoglobinuria.

  1997cites this paper
• Murine embryonic stem cells without pig-a gene activity are competent for hematopoiesis with the PNH phenotype but not for clonal expansion.

  1997cites this paper