A monoclonal antibody specific for immature human hemopoietic cells and T lineage cells.

An anti-human monoclonal antibody (RFB-1) has been produced that reacts with a group of hemopoietic precursor cells in human bone marrow. These include terminal deoxynucleotidyl transferase-positive (TdT+) cells and myeloid colony-forming unit cells, functionally identifiable progenitor cells of the granulocytic-monocytic series. The expression of RFB-1 antigen on myeloid cells decreases as the cells become more mature; myeloblasts are weakly RFB-1+ but most promyelocytes are RFB-1-. RFB-1 also reacts with TdT+ thymic blast cells and cortical thymocytes but is unreactive with TdT- medullary thymocytes, although the majority of peripheral T cells are weakly RFB-1+. RFB-1 is the first anti-precursor cell reagent that labels human TdT+ cells in both the thymus and bone marrow as well as hemopoietic precursor cells but is unreactive with pre-B blasts and B lymphocytes.

• Publication year

  1981

• Venue

  Journal of Immunology

• Publication date

  1981-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.127.6.2269PMID 6975315
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Delineation of human thymocyte differentiation pathways utilizing double-staining techniques with monoclonal antibodies.

  1981cited by this paper
• Diversity of immunoglobulin expression in leukaemic cells resembling B-lymphocyte precursors

  1981cited by this paper
• Induction of terminal deoxynucleotidyl transferase and Lyt antigens with thymosin: identification of multiple subsets of prothymocytes in mouse bone marrow and spleen.

  1981cited by this paper
• Expression of cell-surface HLA-DR, HLA-ABC and glycophorin during erythroid differentiation

  1981cited by this paper
• Distribution of t lymphocyte subsets in the human bone marrow and thymus: an analysis with monoclonal antibodies.

  1981cited by this paper
• Analysis of rat hemopoietic cells on the fluorescence-activated cell sorter. I. Isolation of pluripotent hemopoietic stem cells and granulocyte-macrophage progenitor cells

  1980cited by this paper
• Reappearance of terminal deoxynucleotidyl transferase containing cells in rat bone marrow following corticosteroid administration.

  1980cited by this paper
• Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.

  1980cited by this paper
• A bioassay system for two types of colony stimulating factor in human serum.

  1980cited by this paper
• Strategies for generating monoclonal antibodies defining human t-lymphocyte differentiation antigens.

  1980cited by this paper
• The differentiation and function of human T lymphocytes.

  1980cited by this paper
• A monoclonal antibody to human acute lymphoblastic leukaemia antigen

  1980cited by this paper
• Continuous human bone marrow culture: Ia antigen characterization of probable pluripotential stem cells.

  1980cited by this paper
• Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison

  1980cited by this paper
• Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage.

  1980cited by this paper
• The human thymic microenvironment: an immunohistologic study.

  1980cited by this paper
• A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody

  1979cited by this paper
• Antigenic and enzymatic phenotypes of the pre-B subclass of acute lymphoblastic leukaemia.

  1979cited by this paper
• Terminal transferase-positive human bone marrow cells exhibit the antigenic phenotype of common acute lymphoblastic leukemia.

  1979cited by this paper
• Monoclonal antibodies defining distinctive human T cell surface antigens.

  1979cited by this paper
• Granuloerythropoietic colonies in human bone marrow, peripheral blood, and cord blood.

  1978cited by this paper
• Cell sorter analysis of leukaemia-associated antigens on human myeloid precursors

  1978cited by this paper
• Patterns of gene expression and the cellular origins of human leukaemias.

  1978cited by this paper
• Lymphocytes as Models for the Study of Mammalian Cellular Differentiation

  1977cited by this paper
• Murine pluripotential stem cells lack Ia antigen

  1977cited by this paper
• Antigens on mouse and rat lymphocytes recognized by rabbit antiserum against rat brain: The quantitative analysis of a xenogeneic antiserum

  1975cited by this paper
• Antibody to terminal deoxynucleotidyl transferase.

  1975cited by this paper
• Continuous cultures of fused cells secreting antibody of predefined specificity

  1975cited by this paper
• Human bone marrow colony growth in agar‐gel

  1970cited by this paper

• Population-based targeted RNA sequencing reveals novel disease-related gene fusions in pediatric and adult T-ALL.

  2022cites this paper
• Immunohistochemistry for PAX7 is a useful confirmatory marker for Ewing sarcoma in decalcified bone marrow core biopsy specimens

  2018cites this paper
• Diagnosis of Acute Lymphoblastic Leukemia Originating From T-Lineage Precursors and Approaches to Minimal Residual Disease Monitoring

  2018cites this paper
• surface antigen on human marrow cells and leukemic cell lines Antigenic analysis of hematopoiesis. I. Expression of the My-1 granulocyte

  2011cites this paper
• vitro culture translocation expresses T lymphoid and myeloid surface antigens after in Human acute unclassified leukemia with a unique t(4;17) chromosomal

  2011cites this paper
• Immaturity associated antigens are lost during induction for T cell lymphoblastic leukemia: Implications for minimal residual disease detection

  2010cites this paper
• Reactivity of the monoclonal antibody RFB1 in chronic B-cell leukaemias.

  2009cites this paper
• The Human Thymic Microenvironment

  2008cites this paper
• Distribution of a Hematopoietic-speciflc Differentiation Antigen of K562 Cells in the Human Myeloid and Lymphoid Cell Lineages1

  2006cites this paper
• Immunoreactivity of MIC2 (CD99) and terminal deoxynucleotidyl transferase in bone marrow clot and core specimens of acute myeloid leukemias and myelodysplastic syndromes.

  2006cites this paper
• CD99 expression in T-lineage ALL: implications for flow cytometric detection of minimal residual disease

  2004cites this paper
• Expression cloning of cDNA clones encoding the human cell surface proteins Huly-m6 and FMC29

  2004cites this paper
• CD99 (MIC2) expression in paediatric B‐lineage leukaemia/lymphoma reflects maturation‐associated patterns of normal B‐lymphopoiesis

  1999cites this paper
• Anti‐MIC2 as a tool in examination of testicular biopsies

  1999cites this paper
• Diagnostic utility of MIC‐2 immunocytochemical staining in the differential diagnosis of small blue cell tumors

  1998cites this paper
• The Xg blood group system: a review.

  1998cites this paper
• 013 (CD99) positivity in hematologic proliferations correlates with TdT positivity.

  1997cites this paper
• Anticorps mg1 reconnaissant un petit sous-ensemble de cellules hematopoietiques humaines

  1997cites this paper
• Multiparameter phenotype mapping of normal and post-chemotherapy B lymphopoiesis in pediatric bone marrow

  1997cites this paper
• Selection and transplantation of hematopoietic stem and progenitor cells.

  1994cites this paper
• Immunohistochemical Profile of Monoclonal Antibody 013: Antibody That Recognizes Glycoprotein p30/32MIC2 and is Useful in Diagnosing Ewing's Sarcoma and Peripheral Neuroepithelioma

  1994cites this paper
• Ontogeny of hematopoietic stem cell development: reciprocal expression of CD33 and a novel molecule by maturing myeloid and erythroid progenitors.

  1993cites this paper
• Three neuroblastoma cell lines established from consecutive samples of one patient which show distinct morphologic features, MYCN amplification, and surface marker expression.

  1992cites this paper
• MIC2 is a specific marker for ewing's sarcoma and peripheral primitive neuroectodermal tumors. Evidence for a common histogenesis of ewing's sarcoma and peripheral primitive neuroectodermal tumors from MIC2 expression and specific chromosome aberration

  1991cites this paper
• Immune markers in hematologic malignancies.

  1991cites this paper
• Isolation of an early T-cell precursor (CFU-TL) from human bone marrow.

  1990cites this paper
• The thymus—what's new?

  1989cites this paper
• Laboratory and clinical applications of monoclonal antibodies for leukemias and non-Hodkin's lymphoma

  1989cites this paper
• The MIC2 gene product: epitope mapping and structural prediction analysis define an integral membrane protein.

  1989cites this paper
• Laboratory and clinical applications of monoclonal antibodies for leukemias and non-Hodgkin's lymphomas.

  1989cites this paper
• Cell to cell contact enhances the proliferation of acute myeloid leukemia (AML) cells in vitro without an apparent role of adhesion glycoproteins LFA1, MAC1 and P150-95.

  1989cites this paper
• A monoclonal antibody with reaction spectrum covering acute leukemia and T-lineage cells.

  1988cites this paper
• Effectofthe Antibody on the C3bi-mediated Adherence by Neutrophils and Expressionofthe Antigen During Myelopoiesis

  1988cites this paper
• Expression of NK-cell associated antigen on human fetal liver cells.

  1988cites this paper
• Tamm‐horsfall protein is a marker of renal and extra‐renal rhabdoid tumours

  1988cites this paper
• Immunophenotype of clonogenic cells in myeloid leukaemia.

  1988cites this paper
• A monoclonal antibody to a human neutrophil-specific plasma membrane antigen. Effect of the antibody on the C3bi-mediated adherence by neutrophils and expression of the antigen during myelopoiesis

  1988cites this paper
• Pseudoautosomal genes in man.

  1987cites this paper
• Induction of B-lymphocyte antigens on the chronic myeloid leukemic cell line K562 using sodium butyrate.

  1987cites this paper
• Infrequent Phenotypes of Clonogenic Cells in AML: Evidence for Abnormal Differentiation and Possibilities for Detecting Minimal Disease?

  1987cites this paper
• Interleukin 2 responsiveness of immature T-cell colony-forming cells (T-CFC) from patients with acute T-cell lymphoblastic leukemias.

  1987cites this paper
• Isolation of hemopoietic progenitor cells from human umbilical cord blood.

  1987cites this paper
• A study of cell surface glycoproteins in chronic lymphocytic leukaemia

  1987cites this paper
• Tseudo‐lymphoid‘ leukaemia with unusual features: Ultrastructural, immunological, cytogenetic and molecular studies

  1987cites this paper
• Characterization of interleukin-2 receptors expressed on acute leukemic B cells.

  1987cites this paper
• The purification of human basophils: their immunophenotype and cytochemistry

  1987cites this paper
• Enrichment of human marrow T-cell precursors by complement-dependent cytotoxicity with monoclonal antibodies and Percoll Gradient centrifugation.

  1987cites this paper
• PER-117: a new human ALL cell line with an immature thymic phenotype.

  1987cites this paper
• Distribution of a hematopoietic-specific differentiation antigen of K562 cells in the human myeloid and lymphoid cell lineages.

  1987cites this paper
• In vitro differentiation of a null-acute leukemia: T-lymphoid surface antigen expression associated with rearrangement in T-cell receptor beta chain variable genes.

  1987cites this paper
• Autologous Bone Marrow Transplantation in Acute Myeloblastic Leukemia (AML): In Vitro Studies to Detect Minimal Disease in Remission Marrow

  1987cites this paper
• The quantitation of HLA-DR expression on human cells using immunocytochemistry.

  1987cites this paper
• Human acute unclassified leukemia with a unique t(4;17) chromosomal translocation expresses T lymphoid and myeloid surface antigens after in vitro culture.

  1987cites this paper
• A monoclonal antibody reacting with human basophils.

  1987cites this paper
• The kinetics of terminal deoxynucleotidyl transferase-positive cells in the mixed colony assay.

  1986cites this paper
• Immunologic classification of leukemia and lymphoma.

  1986cites this paper
• Establishment in long term culture of megakaryocytic leukemia cells (EST-IU) from the marrow of a patient with leukemia and a mediastinal germ cell neoplasm.

  1986cites this paper
• Cloning an expressed gene shared by the human sex chromosomes.

  1986cites this paper
• Molecular genetics of MIC2: a gene shared by the human X and Y chromosomes.

  1986cites this paper
• A sensitive and specific ELISA using a monoclonal capture antibody for detection of Tamm-Horsfall urinary glycoprotein in serum.

  1986cites this paper
• Recent advances in the immunologic classification of leukemia.

  1986cites this paper
• Antigenic analysis of hematopoiesis. V. Characterization of My-10 antigen expression by normal lymphohematopoietic progenitor cells.

  1986cites this paper
• An attempt to produce “pre‐T” cell hybridomas and to identify their antigens

  1986cites this paper
• Diversity of cell surface hematopoietic antigens on K-562 sublines identified with monoclonal antibodies.

  1986cites this paper
• Biochemistry and expression of myelomonocytic antigens.

  1986cites this paper
• Cellular differentiation of lymphoid subpopulations and their microenvironments in the human thymus.

  1986cites this paper
• T-cell colony formation in patients with T-cell malignancies: growth factor requirements for in-vitro proliferation of peripheral blood T-cell colony-forming cells.

  1986cites this paper
• Towards Detection of Minimal Disease: Discrimination of AML Precursors from Normal Myeloid Precursors using a Combination of Surface Markers

  1986cites this paper
• Inhibition of CFU-GM, BFU-E, and CFU-GEMM Colony Formation by Monoclonal Antibodies Selected from the Myeloid Panel

  1986cites this paper
• Expression of Myeloid and B Cell-Associated Antigens on T Lineage Cells

  1986cites this paper
• Expression of T Cell-Related Antigens on Cells from the Myelo-Monocytic Lineage

  1986cites this paper
• Reactivity of a Monoclonal Antibody Defining Human Hematopoietic Multipotential Progenitors

  1986cites this paper
• Purification of fetal hematopoietic progenitors and demonstration of recombinant multipotential colony-stimulating activity.

  1985cites this paper
• Biological Markers in Lymphoproliferative Disorders

  1985cites this paper
• Blast crisis of chronic granulocytic leukemia with mast cell and basophilic precursors.

  1985cites this paper
• Monoclonal antibodies against human myelomonocytic cells: detection of certain lineage-specific antigens on CFU-GM progenitor cells.

  1985cites this paper
• Surface markers in chronic lymphoid leukemias of B cell type.

  1985cites this paper
• Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics.

  1985cites this paper
• T-lymphocyte progenitors in man: phenotypic characterization of blood and bone marrow T-colony forming cells.

  1985cites this paper
• Accessory role of autologous T lymphocytes and adherent cells for the in vitro proliferation of T-cell colony-forming cells from patients with T-cell acute lymphoblastic leukemia.

  1985cites this paper
• Monoclonal antibodies against human hemopoietic cells and the separation of progenitor cells from bone marrow.

  1985cites this paper
• Production and analysis of HH 10 monoclonal antibodies reactive to immature hematopoietic cells and their use for monitoring acute leukemia cells.

  1985cites this paper
• Nonlymphoblastic Leukemia-Associated Antigens Identified by Monoclonal Antibodies

  1985cites this paper
• Differentiation capacity of null-AL(L) cells in culture.

  1985cites this paper
• The human Y chromosome.

  1985cites this paper
• Immunodiagnosis and immunotherapy of childhood malignancies.

  1985cites this paper
• A system for accurate immunolocalization of Tamm-Horsfall protein in renal biopsies

  1985cites this paper
• T cell lineage involvement in lymphoid blast crisis of chronic myeloid leukemia.

  1985cites this paper
• Thy‐1 is a differentiation antigen that characterizes immature murine erythroid and myeloid hematopoietic progenitors

  1985cites this paper
• Characterization of blast cells in chronic granulocytic leukaemia in transformation, acute myelofibrosis and undifferentiated leukaemia II. STUDIES WITH MONOCLONAL ANTIBODIES AND TERMINAL TRANSFERASE

  1985cites this paper
• Identification of a membrane glycoprotein associated with haemopoietic progenitor cells.

  1985cites this paper
• Characterization of a murine monoclonal antibody specific for human early lymphohemopoietic cells.

  1984cites this paper
• Monoclonal antibody specific for granulocytic‐lineage cells and reactive with human pluripotent and committed haematopoietic progenitor cells

  1984cites this paper
• Phorbol ester-induced changes in human leukemic pre-B lines.

  1984cites this paper
• Self-renewal and maturation of immature T-cell progenitors within PHA-induced agar T-cell colonies.

  1984cites this paper
• Double fluorochrome analysis of human bone marrow lymphoid cells: studies with terminal transferase and anti-T-cell monoclonal antibodies.

  1984cites this paper
• GM 58/8: a monoclonal antibody that identifies a new lineage‐specific determinant expressed by myeloid progenitors (CFU‐GM) and their progeny

  1984cites this paper
• Preparation and characterization of monoclonal antibodies recognizing two distinct differentiation antigens (Pro-Im1, Pro-Im2) on early hematopoietic cells.

  1984cites this paper
• Cell-surface structures involved in haemopoietic cell differentiation and proliferation.

  1984cites this paper
• Enrichment of pluripotent hemopoietic progenitor cells from human bone marrow.

  1984cites this paper