The catalytic activity of carboxypeptidase-degraded aldolase.

One approach to the general problem of the structure and function of enzymes involves proteolytic hydrolysis with examination of the structure, composition, and catalytic activity of the hydrolysis products. Fragments retaining partial enzyme activity have been obtained from trypsin and pepsin by autolysis (2, 3) and from trypsinogen by peptic digestion (4). Over half of the amino acid residues of papain may be removed by aminopeptidase without activity loss (5). Ribonuclease loses activity when a peptide of 20 residues is removed from the NH&erminal end, but regains activity when recombined with the peptide (6). Removal of COOH-terminal residues by carboxypeptidase has little effect on the activity of chymotrypsin (7), lysozyme (S), or ribonuclease (9, 10). In contrast, studies in this laboratory, preliminary to the investigations reported herein, (II), showed that treatment of muscle aldolase by crude trypsin or carboxypeptidase readily caused marked activity loss. The purpose of this paper is to present observations on the striking decrease in aldolase catalytic activity and concomitant change in specificity accompanying release of terminal tyrosine residues by carboxypeptidase digestion.

• Publication year

  1959

• Venue

  Journal of Biological Chemistry

• Publication date

  1959-10-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(18)69749-2PMID 13818028
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Release of terminal tyrosine residues during carboxypeptidase digestion is accompanied by a change in aldolase specificity.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionAK (4715169a40) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• Carboxypeptidase digestion of muscle aldolase causes a marked loss of catalytic activity.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionAK (4715169a40) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review뀨 (7c402c1b98) reviewq (76h6bfydm6) review

• aldolase

  enzyme

  The glycolytic enzyme examined here for changes in activity and specificity after proteolytic degradation.

  Aliases: aldolase enzyme

  박진우 (dztg5apj7m) extractionAK (4715169a40) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• carboxypeptidase digestion

  process

  Proteolytic treatment with carboxypeptidase used to remove amino acids from the protein's carboxyl terminus.

  Aliases: carboxypeptidase-degradation

  박진우 (dztg5apj7m) extractionAK (4715169a40) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• catalytic activity

  biochemical property

  The enzymatic activity of aldolase measured as its ability to catalyze its reaction.

  Aliases: enzyme activity

  박진우 (dztg5apj7m) extractionAK (4715169a40) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• muscle aldolase

  enzyme preparation

  The aldolase preparation isolated from muscle and used as the substrate for carboxypeptidase treatment.

  Aliases: muscle enzyme aldolase

  박진우 (dztg5apj7m) extractionAK (4715169a40) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• substrate specificity

  biochemical property

  The pattern of substrates or reaction behavior recognized by aldolase in catalysis.

  Aliases: specificity

  박진우 (dztg5apj7m) extractionAK (4715169a40) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• terminal tyrosine residues

  amino acid residues

  Tyrosine amino acids located at the carboxyl terminus of the aldolase molecule and released by digestion.

  Aliases: COOH-terminal tyrosine residues, C-terminal tyrosine residues

  박진우 (dztg5apj7m) extractionAK (4715169a40) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review뀨 (7c402c1b98) reviewq (76h6bfydm6) review

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