CRISPR Knockouts in Ciona Embryos.

No abstract is available for this paper.

• Publication year

  2018

• Venue

  Advances in Experimental Medicine and Biology

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1007/978-981-10-7545-2_13PMID 29542087PMCID PMC6061950
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Efficient CRISPR/Cas9-based gene knockout in watermelon

  2017cited by this paper
• CRISPR/Cas9 genome editing of rubber producing dandelion Taraxacum kok-saghyz using Agrobacterium rhizogenes without selection

  2016cited by this paper
• A CRISPR/Cas9 system adapted for gene editing in marine algae

  2016cited by this paper
• Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

  2016cited by this paper
• Rewiring of an ancestral Tbx1/10-Ebf-Mrf network for pharyngeal muscle specification in distinct embryonic lineages

  2016cited by this paper
• Fibronectin contributes to notochord intercalation in the invertebrate chordate, Ciona intestinalis

  2016cited by this paper
• Engineered CRISPR-Cas9 nucleases with altered PAM specificity

  2016cited by this paper
• Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona

  2016influential reference
• Efficient and Heritable Targeted Mutagenesis in Mosses Using the CRISPR/Cas9 System.

  2016cited by this paper
• Analysis of Noncanonical Calcium-Dependent Protein Kinases in Toxoplasma gondii by Targeted Gene Deletion Using CRISPR/Cas9

  2016cited by this paper
• Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

  2015cited by this paper
• Engineered CRISPR-Cas9 nucleases with altered PAM specificities

  2015cited by this paper
• Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.

  2015cited by this paper
• In Silico Predictive Modeling of CRISPR/Cas9 guide efficiency

  2015cited by this paper
• Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers

  2015cited by this paper
• Mitotic Membrane Turnover Coordinates Differential Induction of the Heart Progenitor Lineage.

  2015cited by this paper
• CRISPRscan: designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo

  2015cited by this paper
• CRISPR/Cas9‐mediated genome modification in the mollusc, Crepidula fornicata

  2015cited by this paper
• T-type Calcium Channel Regulation of Neural Tube Closure and EphrinA/EPHA Expression.

  2015cited by this paper
• Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

  2014cited by this paper
• Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9

  2014influential reference
• Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases

  2014cited by this paper
• Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona

  2014cited by this paper
• Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

  2014cited by this paper
• Detecting and Quantifying Low Level Gene Variants in Sanger Sequencing Traces Using the ab1 Peak Reporter Tool.

  2014cited by this paper
• CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis

  2014cited by this paper
• CRISPR RNA-guided activation of endogenous human genes

  2013cited by this paper
• One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.

  2013cited by this paper
• RNA-Guided Human Genome Engineering via Cas9

  2013cited by this paper
• RNA-programmed genome editing in human cells

  2013cited by this paper
• Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system.

  2013cited by this paper
• Multiplex Genome Engineering Using CRISPR/Cas Systems

  2013cited by this paper
• Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression.

  2013cited by this paper
• RNA-guided gene activation by CRISPR-Cas9-based transcription factors

  2013cited by this paper
• A genomic overview of short genetic variations in a basal chordate, Ciona intestinalis

  2012cited by this paper
• A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

  2012cited by this paper
• Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

  2012cited by this paper
• Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc‐finger nucleases

  2012cited by this paper
• CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

  2011cited by this paper
• Widespread occurrence of non-canonical transcription termination by human RNA polymerase III

  2011cited by this paper
• A TALE nuclease architecture for efficient genome editing

  2011cited by this paper
• The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

  2010cited by this paper
• Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

  2010cited by this paper
• Electroporation of transgenic DNAs in the sea squirt Ciona.

  2009cited by this paper
• Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.

  2008cited by this paper
• RNA interference by expressing short hairpin RNA in the Ciona intestinalis embryo

  2008cited by this paper
• CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

  2007cited by this paper
• Enhancing Gene Targeting with Designed Zinc Finger Nucleases

  2003cited by this paper
• Engineering polydactyl zinc-finger transcription factors

  2002cited by this paper
• A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR.

  1997influential reference

• Neuron-specific Agrin splicing by Nova RNA-binding proteins regulates conserved neuromuscular junction development in chordates

  2025cites this paper
• A protocadherin mediates cell-cell adhesion and integrity of the oral placode in the tunicate Ciona

  2025cites this paper
• A conserved RNA switch for acetylcholine receptor clustering at neuromuscular junctions in chordates

  2024cites this paper
• Specification and survival of post-metamorphic branchiomeric neurons in a non-vertebrate chordate

  2024cites this paper
• Distribution of cionin, a cholecystokinin/gastrin family peptide, and its receptor in the central nervous system of Ciona intestinalis type A

  2024cites this paper
• Optimizing CRISPR/Cas9 approaches in the polymorphic tunicate Ciona intestinalis.

  2024cites this paper
• A gene regulatory network for specification and morphogenesis of a Mauthner Cell homolog in non-vertebrate chordates

  2024cites this paper
• Reaping the benefits of liquid handlers for high-throughput gene expression profiling in a marine model invertebrate

  2024cites this paper
• CRISPR/Cas9 protocols for disrupting gene function in the non-vertebrate chordate Ciona.

  2024cites this paper
• Ring Finger 149-Related Is an FGF/MAPK-Independent Regulator of Pharyngeal Muscle Fate Specification

  2023cites this paper
• Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function

  2023influential citation
• Specification and survival of post-metamorphic branchiomeric neurons in the hindbrain of a non-vertebrate chordate

  2023cites this paper
• Loss of collagen gene expression in the notochord of the tailless tunicate Molgula occulta.

  2023cites this paper
• Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function

  2023cites this paper
• Pax3/7 regulates neural tube closure and patterning in a non-vertebrate chordate

  2022cites this paper
• Marine animal evolutionary developmental biology—Advances through technology development

  2022cites this paper
• Rnf149-related is an FGF/MAPK-independent regulator of pharyngeal muscle fate specification

  2022cites this paper
• CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin.

  2021cites this paper
• A preliminary investigation into the role of Sipa1l in bipolar tail neurons of Ciona robusta

  2021cites this paper
• Evolution of a chordate-specific mechanism for myoblast fusion

  2021cites this paper
• Regulation of Neurogenesis by FGF Signaling and Neurogenin in the Invertebrate Chordate Ciona

  2020cites this paper
• Effector gene expression underlying neuron subtype-specific traits in the Motor Ganglion of Ciona.

  2020cites this paper
• The Cis-Regulatory Code for Kelch-like 21/30 Specific Expression in Ciona robusta Sensory Organs

  2020cites this paper
• Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A

  2020cites this paper
• Osmoregulation in Barnacles: An Evolutionary Perspective of Potential Mechanisms and Future Research Directions

  2019cites this paper