Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus

BackgroundReceptor-like kinases are well-known to play key roles in disease resistance. Among them, the Wall-associated kinases (WAKs) have been shown to be positive regulators of fungal disease resistance in several plant species. WAK genes are often transcriptionally regulated during infection but the pathways involved in this regulation are not known. In rice, the OsWAK gene family is significantly amplified compared to Arabidopsis. The possibility that several WAKs participate in different ways to basal defense has not been addressed. Moreover, the direct requirement of rice OSWAK genes in regulating defense has not been explored.ResultsHere we show using rice (Oryza sativa) loss-of-function mutants of four selected OsWAK genes, that individual OsWAKs are required for quantitative resistance to the rice blast fungus, Magnaporthe oryzae. While OsWAK14, OsWAK91 and OsWAK92 positively regulate quantitative resistance, OsWAK112d is a negative regulator of blast resistance. In addition, we show that the very early transcriptional regulation of the rice OsWAK genes is triggered by chitin and is partially under the control of the chitin receptor CEBiP. Finally, we show that OsWAK91 is required for H2O2 production and sufficient to enhance defense gene expression during infection.ConclusionsWe conclude that the rice OsWAK genes studied are part of basal defense response, potentially mediated by chitin from fungal cell walls. This work also shows that some OsWAKs, like OsWAK112d, may act as negative regulators of disease resistance.

• Publication year

  2016

• Venue

  BMC Plant Biology

• Publication date

  2016-01-16

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s12870-016-0711-xPMID 26772971PMCID 4715279
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Plant Pattern Recognition Receptors

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• A maize wall-associated kinase confers quantitative resistance to head smut

  2014influential reference
• Plant PRRs and the activation of innate immune signaling.

  2014cited by this paper
• Requirement for Pectin Methyl Esterase and Preference for Fragmented over Native Pectins for Wall-associated Kinase-activated, EDS1/PAD4-dependent Stress Response in Arabidopsis*

  2014cited by this paper
• Immune receptor complexes at the plant cell surface.

  2014cited by this paper
• Plant pattern-recognition receptors.

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• CEBiP is the major chitin oligomer-binding protein in rice and plays a main role in the perception of chitin oligomers

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• Transcriptomics-based screen for genes induced by flagellin and repressed by pathogen effectors identifies a cell wall-associated kinase involved in plant immunity

  2013influential reference
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  2013influential reference
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  2012cited by this paper
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  2012cited by this paper
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• RNAi mediated silencing of a wall associated kinase, OsiWAK1 in Oryza sativa results in impaired root development and sterility due to anther indehiscence

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• Perception of the chitin oligosaccharides contributes to disease resistance to blast fungus Magnaporthe oryzae in rice.

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• Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice

  2010influential reference
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  2009influential reference
• ErbB receptors and signaling pathways in cancer.

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• A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors.

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• Pectin activation of MAP kinase and gene expression is WAK2 dependent.

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• Activation of defense response pathways by OGs and Flg22 elicitors in Arabidopsis seedlings.

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• Bacterial disease resistance in Arabidopsis through flagellin perception

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• High throughput T-DNA insertion mutagenesis in rice: a first step towards in silico reverse genetics.

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• Comparative Analysis of the Receptor-Like Kinase Family in Arabidopsis and Rice

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• Identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1

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  2002cited by this paper
• Ligand-induced, receptor-mediated dimerization and activation of EGF receptor.

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• Interaction of the Arabidopsis Receptor Protein Kinase Wak1 with a Glycine-rich Protein, AtGRP-3*

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  1984cited by this paper

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